Carrier rate of the c.235G>C mutation in the bovine isoleucyl-tRNA synthetase (IARS) gene of Japanese Black cows at Kagoshima prefecture, Japan,and analysis of the metabolic profiling and reproductive performance of heterozygous cows     

Md Shafiqul ISLAM  Urara SHINYA  Mitsuhiro TAKAGI  Takao AKAHOSHI  Akira YABUKI  Shahnaj PERVIN  Tofazzal Md RAKIB  Mohammad Mahbubur RAHMAN  Martia Rani TACHARINA  Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):254

Bovine isoleucyl-tRNA synthetase (IARS) disorder, a major cause of weak calf syndrome, is caused by a homozygous missense (c.235G>C) mutation in the bovine IARS gene of Japanese Black (JB) cattle, which was identified in 2013. However, the extent to which the carrier rate has changed at Kagoshima prefecture, Japan, and whether the carrier status is associated with any clinical or reproductive problems, have yet to be ascertained. In this study, using a real-time polymerase chain reaction-based genotyping assay, we determined the carrier rate in a regional JB cow population at Kagoshima prefecture. Comparative analyses were performed on the metabolic profile test (MPT) results and reproductive performance data obtained for heterozygous carrier and homozygous wild-type cows. In 2009 and 2018, DNA samples were collected from 130 and 462 clinically healthy JB cows, respectively, in Kagoshima prefecture. MPT results and reproductive performance data were evaluated for 62 cows, comprising four heterozygous carriers and 58 wild-type cows. Genotyping revealed that the carrier rate was 6.9% in 2009 and 1.5% in 2018, the difference of which was statistically significant (P<0.005). There were no statistically significant differences between the carrier and wild-type cows with respect to either MPT results or reproductive performance, indicating that the carrier cows have necessary IARS activity to maintain minimal health and reproductive potential.  相似文献   

New technique of sex preselection for increasing female ratio in boar sperm model     

Yoo-Jin Park  Ki-Jin Kwon  Won-Hee Song  Won-Ki Pang  Do-Yeal Ryu  Md Saidur Rahman  Myung-Geol Pang 《Reproduction in domestic animals》2021,56(2):333-341

In this study, we tried to optimize the porcine semen extender conditions to maximize the differences between live X chromosome-bearing (X) spermatozoa and to Y chromosome-bearing (Y) spermatozoa without a decline in the fertility rate at different pH conditions during storage. We observed the viability of X and Y boar spermatozoa in acidic (pH 6.2), original (pH 7.2), and alkaline condition (pH 8.2) for 5 days to investigate the effect of storage conditions on the X to Y spermatozoa ratio. The functional parameters of spermatozoa were also examined to evaluate sperm quality. Sperm motility was preserved at pH 7.2 and pH 6.2 for 3 days, while sperm motility at pH 8.2 decreased significantly after 2 days. Non-capacitated spermatozoa increased while capacitated spermatozoa decreased during storage. Sperm viability decreased significantly duration-dependent under all pH conditions, but there was no significant difference during storage at pH 6.2 and 7.2. The X: Y ratio of live spermatozoa in acidic condition was maximized (1.2:1) without affecting the sperm function and fertility-related protein expression after 2 days compared to original conditions. Moreover, insemination of sows using acidic extender increased the number of female pups on days 1 and 2 of preservation. These results indicate that the production of female offspring may increase when acidic BTS is used for 2 days without affecting the success rate of AI. Above all, this method is simple and economical compared to other methods.  相似文献   

Molecular characterisation of Theileria equi and risk factors associated with the occurrence of theileriosis in horses of Punjab (Pakistan)     

S. Ali  M. Ijaz  S. H. Farooqi  A. Z. Durrani  M. I. Rashid  A. Ghaffar  A. Ali  A. Rehman  S. Aslam  I. Khan  A. Masud  K. Mehmood 《Equine Veterinary Education》2021,33(2):75-83

Theileria equi (T. equi) is an obligate intra- and extra-erythrocytic parasite that causes equine theileriosis (ET) in equids. Equine theileriosis is considered a notifiable disease of global significance, a major constraint to the international movement of horses, and endemic in many countries. This disease may be difficult to diagnose, as it can produce variable and nonspecific clinical signs. A cross-sectional study was designed for the molecular characterisation of T. equi and to investigate the associated risk factors of ET accompanied by its consequences on haematological and sero-biochemical parameters. A convenience sampling of 500 blood samples were collected from ET suspect horses from January to December 2017. PCR was performed on all blood samples targeting the 18S rRNA gene of T. equi followed by sequencing; 9% animals tested positive with confirmed sequences. The isolates of this study showed high homology with Cuban, Russian and Brazilian isolates of T. equi (accession numbers KY111762.2 , MG551915.1 and KY952237.1 , respectively). Based on multivariate analysis, the principal risk factors consisted of absence of dogs on the premises and presence of tick infestation. The haemato-biochemical parameters showed a decrease in granulocytes and erythrocytes, and an increase in lymphocytes, monocytes, mean corpuscular volume, mean corpuscular haemoglobin, mean platelet volume, glucose, phosphorus and aspartate aminotransferase in positive horses. This is the first study which identified ET in Punjab (Pakistan) using molecular techniques and risk factors together with the haemato-biochemical variations in horses.  相似文献   

MiPDCD6 effector suppresses host PAMP-triggered immunity to facilitate Meloidogyne incognita parasitism in tomato     

Md Kamaruzzaman  Li-Feng Zhao  Jin-Ai Zhang  Le-Tian Zhu  Yuan Li  Xiao-Da Deng  Shu-Jing Cai  Xiao-Cong Lian  Fang-Ni Chen  Ning Jia  Xinrong Wang 《Plant pathology》2023,72(1):195-206

  相似文献   

Towards developing resistance to chickpea chlorotic dwarf virus through CRISPR/Cas9-mediated gene editing using multiplexed gRNAs     

Malik  Muniba Abid Munir  Haider  Muhammad Saleem  Zhai  Ying  Khan  Muhammad Azmat Ullah  Pappu  Hanu R. 《植物病害和植物保护杂志》2023,130(1):23-33

Journal of Plant Diseases and Protection - Mastreviruses are an emerging group of viruses transmitted by leafhoppers and infect both monocot and dicot plants. Chickpea chlorotic dwarf virus...  相似文献   

Current status and prospects of farming the giant river prawn Macrobrachium rosenbergii (De Man) in Bangladesh     

Md Abdul Wahab  Sk Ahmad‐Al‐Nahid  Nesar Ahmed  Mohammad M Haque  Mahmudul Karim 《Aquaculture Research》2012,43(7):970-983

Giant river prawn (Macrobrachium rosenbergii) farming plays an important role in the economy of Bangladesh. Presently, it is cultured in around 50 000 ha area with total annual production of 23 240 t. Traditional extensive prawn farming has been expanding over the last three decades through the introduction and adoption of improved culture systems, such as culture of prawn‐carps, prawn‐shrimp‐fish and prawn‐fish‐rice as concurrent and rotational systems. Efforts for the development of improved techniques on broodstock management, seed production and rearing and grow‐out of prawn have been made over the last decade. The outcomes are low‐cost feed for broodstock, production of post‐larvae in net cages (hapa), all‐male prawn culture, periphyton based prawn‐tilapia culture, C/N based prawn culture, organic prawn farming, prawn‐mola culture and prawn‐carp‐mola polyculture. Despite the development of culture technologies, a number of challenges for sustainable development of prawn farming need to be overcome to realize the potentials of this promising sector. Good aquaculture practises at all levels and application of measures for quality control and food safety would ensure sustainable development of prawn farming in Bangladesh.  相似文献   

Effects of salinity on the growth and proximate composition of selected tropical marine periphytic diatoms and cyanobacteria     

Helena Khatoon  Sanjoy Banerjee  Fatimah Md Yusoff  Mohamed Shariff 《Aquaculture Research》2010,41(9):1348-1355

Marine periphytic cyanobacteria and diatoms have been examined as a potential source of feed supplement for rearing aquatic larvae in the aquaculture industry. Culture of the periphytic diatom Amphora sp., Navicula sp., Cymbella sp. and the cyanobacteria Oscillatoria sp. at different salinities showed significant changes in biomass and specific growth rates. Diatoms growth was significantly higher at 35 g L⁻¹, while for cyanobacteria growth was better at 25 g L⁻¹. Significantly higher levels of protein and lipid were found in diatoms at low salinities (15–25 g L⁻¹) and an increase in carbohydrate at high salinities (30–35 g L⁻¹). Conversely, cyanobacteria showed a significantly higher lipid content at 30–35 g L⁻¹ compared with other salinity levels but no significant changes were observed in the protein and carbohydrate contents at different salinity levels. The present findings can be taken into consideration when culturing marine periphytic Amphora sp., Navicula sp., Cymbella sp. and Oscillatoria sp. to provide appropriate levels of protein, lipid and carbohydrate as feed supplement as well as for bioremediation in aquaculture.  相似文献   

Boron Induced Oxidative Stress,Antioxidant Defence Response and Changes in Artemisinin Content in Artemisia annua L.     

T. Aftab  M. M. A. Khan  M. Idrees  M. Naeem  M. Ram 《Journal of Agronomy and Crop Science》2010,196(6):423-430

Boron is an essential plant micronutrient and the range between deficient and toxic levels of boron is narrow for most of the plants. Like other elements, boron becomes toxic to growth at high concentrations. High boron concentrations in soil reduce crop productivity in many areas of the world. The effect of increasing levels of boron (0, 0.50, 1.00, 1.50, 2.00 mm ) on oxidative stress, antioxidant defence response and changes in artemisinin content in Artemisia annua were investigated in the present study. Boron toxicity reduced the growth parameters viz. stem height, fresh weight and dry weight. Treatments induced oxidative stress resulting in lower net photosynthetic rate, stomatal conductance, internal CO₂ and total chlorophyll content. The increased activities of antioxidant enzymes like CAT, POX and SOD were also noted in response to increasing levels of boron stress. However, H₂O₂ and artemisinin content were found to be high up to 1.00 mm concentration of boron compared to control, and on applying higher doses, further reduced contents were obtained. Thus, the results suggest that a mild stress of boron can be utilized for enhanced artemisinin production.  相似文献   

Renal failure associated with ingestion of grapes or raisins in dogs     

Gwaltney-Brant S  Holding JK  Donaldson CW  Eubig PA  Khan SA 《Journal of the American Veterinary Medical Association》2001,218(10):1555-1556

  相似文献   

Serological status for Chlamydophila psittaci, Newcastle disease virus, avian polyoma virus, and Pacheco disease virus in scarlet macaws (Ara macao) kept in captivity in Costa Rica     

Herrera I  Khan SR  Kaleta EF  Müller H  Dolz G  Neumann U 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2001,48(10):721-726

  相似文献