Feline interleukin-8 expression in peripheral blood mononuclear cells induced by egg white derivatives     

Yang MP  Lee KJ  Yun SM  Kim JH  Ko IK  Jeung EB 《Veterinary immunology and immunopathology》2002,86(1-2):43-53

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Retrospective study of canine cutaneous tumors in Korea     

Bidur Pakhrin  Min-Soo Kang  Il-Hong Bae  Mi-Sun Park  Hyang Jee  Mi-Hyeon You  Jae-Hoon Kim  Byung-Il Yoon  Yang-Kyu Choi  Dae-Yong Kim 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(3):229-236

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Hepatocellular adenoma in a Eurasian otter (Lutra lutra)     

Il-Hong Bae  Bidur Pakhrin  Hyang Jee  Nam-Shik Shin  Dae-Yong Kim 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):103-105

A 7-year-old female Eurasian otter (Lutra lutra) at the Seoul Grand Park, Korea, died after displaying depression, anorexia, weight loss and rough skin for several days. At necropsy, a solitary friable round mass, which was approximately 12 × 9 × 5 cm and mottled dark red and yellow, was found bulging from the right hepatic lobe. Microscopically, the nonencapsulated, poorly circumscribed mass was composed of solid sheets of neoplastic hepatocytes. In addition, numerous small tan foci, ranging from 0.5 to 1.0 cm in diameter, were evenly scattered throughout the pancreatic tissue. These foci were found to be nonencapsulated, well-demarcated hyperplastic nodules of the exocrine pancreatic gland. We observed neither intrahepatic nor extrahepatic metastases. Based on the gross and microscopic changes, we diagnosed the animal as having a hepatocellular adenoma accompanied by exocrine pancreatic nodular hyperplasia.  相似文献   

Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island     

Song-Hak Lee  Sang-Chul Kang  Dae-Yong Kim  Jong-Hee Bae  Jae-Hoon Kim 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):51-55

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Fecal microbiome in dogs with lymphoid and nonlymphoid tumors     

Hyeona Bae  Seul Ki Lim  Hee Eun Jo  Yeonsu Oh  Jinho Park  Hak-Jong Choi  DoHyeon Yu 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):648-659

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Genitourinary rhabdomyosarcoma with systemic metastasis in a young dog     

Bae IH  Kim Y  Pakhrin B  You MH  Hwang CY  Kim JH  Kim DY 《Veterinary pathology》2007,44(4):518-520

A 2-year-old intact female Golden Retriever presented due to rapidly progressing depression, ascites, dysuria, abdominal pain, and severe vaginal bleeding. At necropsy, the retroperitoneal space was expanded by multiple coalescing neoplastic nodules and the uterine wall was thickened with poorly defined neoplastic infiltrates. The urinary bladder was markedly thickened due to botryoid nodules exhibiting exophytic growth into the lumen. Metastases to lung, liver, kidney, and abdominal and thoracic lymph nodes were also noted. Microscopically, the genital tract and retroperitoneal masses were consistent with the alveolar subtype of rhabdomysarcoma, while the urinary bladder mass had characteristics of the embryonal subtype. Immunohistochemically, the neoplastic cells in all these tissue sites were intensely positive for desmin, sacromeric actin, and vimentin, while they were uniformly negative for cytokeratin and smooth muscle actin. Phosphotungstic acid hematoxylin stain revealed cross-striations in the cytoplasm of scattered neoplastic cells. Based on the gross findings, histopathology, and immunohistochemistry, genitourinary rhabdomyosarcoma with multisystemic metastases was made.  相似文献   

In vitro maturation of oocytes derived from the brown bear (Ursus arctos)     

Yin XJ  Lee HS  Choi EG  Yu XF  Park GY  Bae I  Yang CJ  Oh DH  Kim NH  Kong IK 《The Journal of reproduction and development》2007,53(3):685-690

This study was conducted to determine whether meiotic maturation could be induced in ovarian oocytes from the American brown bear (Ursus arctos), a model for gamete "rescue" techniques for endangered ursids. The bears were euthanized, and their ovaries were transported to the laboratory within 4 h. The mean ovarian size was 2.4 x 1.8 cm (range: 2.0-3.3 x 1.5-2.2 cm). The ovaries obtained from the 2 brown bears yielded 97 oocytes (48.5/female), and 88 (90.7%) of them were morphologically classified as normal quality. Oocytes were in vitro matured at 38.5 C in 5% CO2 for 24 or 48 h in TCM-199 supplemented with 10% FBS, 1 microg/ml estradiol-17beta, and 10 microg/ml FSH. In Exp. 1, morphologic evaluation of matured oocytes was conducted by measuring the diameters of oocytes with a zona pellucida (ZP) or cytoplasm without a ZP. In Exp. 2, activation was induced by applying two 20 microsec DC pulses of 2.0 kV/cm delivered by an Electro Cell Fusion Generator. The activated oocytes were cultured in TCM-199 containing 2 mM of 6-dimethylaminopurine for 4 h, in Charles Rosenkrans (CR) 1 for 3 days and the in CR2 for another 4 days. The diameters of the matured bear oocytes with a ZP and with cytoplasm without a ZP (161.8 +/- 6.0 and 135.3 +/- 7.5 microm, respectively) were significantly (P<0.05) larger than those of bovine oocytes (150.7 +/- 4.9 and 118.7 +/- 7.5 microm). The maturation rates of the bear oocytes were 17.6 and 59.4% at 24 and 48 h of in vitro maturation, the percentage of activated oocytes that developed to the 2 or 4-cell stage was 31.6%; however, no blastocysts were observed. These results indicate that bear oocytes can develop to metaphase II in an in vitro culture system and that activated oocytes can develop to the 2 or 4-cell stages.  相似文献   

Physical Properties of Extruded Strands of Hydroxypropylated Normal and High-Amylose Corn Starch     

Sang Ouk Bae  Seung-Taik Lim 《Cereal Chemistry》1998,75(4):449-454

Normal (25% amylose) and high-amylose (70% amylose) corn starches (CS and HA) were hydroxypropylated to 0.1 degree of molar substitution (MS) with propylene oxide in an alkaline-ethanol medium (70% ethanol). CS and hydroxypropylated corn starch (HPCS) were mixed on dry basis with water and glycerol at a weight ratio of 7:2:1, and HA and hydroxypropylated high amylose corn starch (HPHA) were mixed at 7:3:1. Stearic acid, glycerol monostearate, or lecithin (3%, based on starch) was added to each mixture to examine the effects on the physical properties of the extrudate. The starch mixtures were extruded at high shear (100 rpm) to nonexpanded strands using a corotating twin-screw extruder in a temperature range of 75–90°C. HA, alone and with all additives, showed lower die swelling in extrusion than did CS, whereas HPCS and HPHA showed higher die swelling than the corresponding unmodified starches. Water absorption of all HA extrudates was lower than those of all CS extrudates (22–35% and 68–97%, respectively, at 25°C). Hydroxypropylation increased the absorption for both starches. All extruded starches, regardless of additives, showed low solubility in water (0.1–1.0% for 2 hr at 25°C). Differential scanning calorimetry indicated that during extrusion, the lipid additives formed a helical complex with amylose in CS and HA, but weakly with HPCS and HPHA. The extruded strands of HA, alone and with additives, exhibited higher tensile and bending strengths (37.1–58.4 and 2.16–5.07 MPa, respectively), compared to the CS strands (12.5–59.3 and 1.06–4.10 MPa, respectively) at the same moisture content (7.5–8.5%). Both tensile strength and percent of elongation of the starch strands were reduced by the presence of a lipid additive. Hydroxypropylation increased elongation and flexibility of the extrudates. HPHA exhibited the greatest mechanical strength and flexibility among the tested starches.  相似文献   

Nutrient concentrations in roots, leaves and wood of seedling and mature sugar maple and American beech at two contrasting sites     

Byung Bae Park  Ruth D. Yanai   《Forest Ecology and Management》2009,258(7):1153-1160

Differences in sensitivity to soil conditions across tree species and developmental stage are important to predicting forest response to environmental change. This study was conducted to compare elemental concentrations in leaves, stems, and roots of (1) sugar maple (Acer saccharum Marsh.) seedlings vs. mature trees and (2) mature sugar maple vs. mature American beech (Fagus grandifolia Ehrh.) in two sites that differ in soil base saturation and pH. Both sites are located in Huntington Forest, NY, USA; one site (hereafter ‘H’) has higher soil pH and Ca, Mg, and Mn concentrations than the other site (hereafter ‘L’). Sugar maple growth at H (14.8 cm² year⁻¹ per tree) was much greater than at L (8.6 cm² year⁻¹ per tree), but the growth of beech was not different between the two sites. Leaves, roots, and stem wood of mature beech trees and sugar maple seedlings and mature trees were sampled for nutrient analysis. Foliar Ca, K, and Al concentrations were positively correlated with soil elements, but Mn concentrations were negatively correlated. Sugar maple differed more than beech between sites in foliar K and Mn concentrations. Root Mg and P concentrations reflected soil chemistry differences, in contrast to foliar concentrations of Mg and P, which were indistinguishable between the sites. In sugar maple, seedlings differed more than in mature trees in nutrient concentrations in roots, especially for Mg and Mn. Although beech was not as responsive to nutrient availability as sugar maple in foliar and root nutrient concentrations, Ca and Mg concentrations in beech wood were higher in H (52% higher for Ca and 68% higher for Mg), while sugar maple did not differ between sites. Sugar maple regeneration failure on acidic soils in the same region is consistent with our finding that sugar maple seedlings were very sensitive to nutrient availability. This sensitivity could ultimately contribute to the replacement of sugar maple by American beech in regions of low pH and base cations if base cation leaching by anthropogenic deposition and tree harvesting continues.  相似文献   

An anion sensor based on calix[4]arene-Reichardt’s dye     

Jin-Seok Bae  Seon-Yeong Gwon  Shinya Matsumoto  Young-A Son  Sung-Hoon Kim 《Fibers and Polymers》2009,10(6):858-860

A highly selective colorimetric displacement assay for anions, F⁻ and H₂PO₄ ⁻, based on calix[4]arene-Reichardt’s dye was studied in DMSO. When calix[4]arene forms a complex with Reichardt’s, the absorption peak at 386, 419 and 632 nm disappeared. Addition of anions to solution of calix[4]arene-Reichardt’s dye causes the blue color of Reichardt’s dye to return as it is displaced from the calix[4]arene. These results demonstrate that this displacement assay can be favorably utilized for the development of potential sensors for the detection of anion such as F⁻ and H₂PO₄ ⁻ in relation to the other anions.  相似文献