Genetic and pathogenic characterization of Akabane viruses isolated from cattle with encephalomyelitis in Korea     

Oem JK  Yoon HJ  Kim HR  Roh IS  Lee KH  Lee OS  Bae YC 《Veterinary microbiology》2012,158(3-4):259-266

A large-scale outbreak of Akabane viral encephalomyelitis in cattle was reported in the southern part of Korea in 2010. Fifteen Akabane virus (AKAV) strains were isolated from the brain and spinal cord samples by using BHK-21 and/or HmLu-1 cells. To examine the genetic relationships and characteristics of the isolates, nucleotide sequences of the S, M, and L segments of the 15 isolates were determined and analyzed. Complete sequence analysis of the 15 AKAV isolates showed 99.9-100% amino acid identities, indicating that the 15 isolates originated from a single strain. The S and M RNA segments of a representative isolate (AKAV-7/SKR/2010) were also compared with the segments of representative reference sequences. This AKAV-7/SKR/2010 strain showed the highest identity with the Iriki and KM-1/Br/06 strains. Neighbor-joining phylogenetic trees of S and M RNA segments were constructed. Four representative AKAV isolates were classified into subgroup Ia, which contains the Iriki and KM-1/Br/06 strains recognized to cause encephalomyelitis in calves and adult cattle in Japan. Moreover, experimental intraperitoneal infection was performed using the AKAV-7/SKR/2010 and AKAV-17/SKR/2010 strains to assess pathogenesis in suckling mice. The 2 isolates, genetically related to the Iriki strain, were neurovirulent and caused neurological signs in suckling mice. In contrast, the 93FMX strain and the K0505 strain, related to the OBE-1 strain, were avirulent in mice. The present results indicate that these isolates most likely had originated from the Iriki strain and are closely related to the Iriki strain both genetically and pathogenically.  相似文献   

Genotypic prevalence of the fimbrial adhesins (F4, F5, F6, F41 and F18) and toxins (LT, STa, STb and STx2e) in Escherichia coli isolated from postweaning pigs with diarrhoea or oedema disease in Korea     

Kwon D  Choi C  Jung T  Chung HK  Kim JP  Bae SS  Cho WS  Kim J  Chae C 《The Veterinary record》2002,150(2):35-37

A PCR was used to determine the genotypic prevalence of five fimbrial adhesins (F4, F5, F6, F41 and F18), two heat-stable enterotoxins (STa and STb), the heat-labile enterotoxin (LT), and the shiga toxin 2e (Stx2e) in 230 isolates of Escherichia coli from postweaning pigs with diarrhoea or oedema disease. Ninety-four (40.9 per cent) of the isolates carried genes for at least one of the fimbrial adhesins or toxins. Genes for the F18 fimbrial adhesin were detected in 18.3 per cent, and genes for F4, F6, F5 and F41 were detected in 10.0 per cent, 4.3 per cent, 1.7 per cent and 0.8 per cent of the isolates, respectively. Genes for STa, STb and LT were detected in 25.7 per cent, 15.2 per cent and 8.7 per cent of the isolates, respectively. Genes for Stx2e were detected in 36 (15.6 per cent) of the isolates, and among them 24 also contained the gene for F18ab and four also contained the gene for F18ac.  相似文献   

Therapeutic monitoring of rivaroxaban in dogs using thromboelastography and prothrombin time     

Junwoo Bae  Hyunwoo Kim  Woosun Kim  Suhee Kim  Jinho Park  Dong‐In Jung  Dohyeon Yu 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(3):1322-1330

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Feed contaminated with classical swine fever vaccine virus (LOM strain) can induce antibodies to the virus in pigs     

Kim B  Song JY  Tark DS  Lim SI  Choi EJ  Kim J  Park CK  Lee BY  Wee SH  Bae YC  Lee OS  Kwon JH  Kang WC  Kim TY  Kim JH  Lee JH  Kang MI 《The Veterinary record》2008,162(1):12-17

In November 2004, antibodies to classical swine fever virus (csfv) were detected in finishing pigs during the annual serological surveillance in Jeju Province, Korea. In addition, csf vaccine viruses (lom strain) had recently been isolated from pigs raised on farms known to have csfv antibody-positive pigs. In contrast with mainland Korea, Jeju Province had been csf free and its pigs had not been vaccinated against csf for more than five years. An epidemiological investigation team from the National Veterinary Research and Quarantine Service investigated the current status of csf prevention on the Korean mainland and in Jeju Province to determine possible routes of introduction of the virus into the province. It was concluded that improperly processed blood meals, manufactured on mainland Korea, had been contaminated with the csf vaccine lom strain, and that the lom strain had been transmitted to pigs fed feed or feedstuffs containing the contaminated meal.  相似文献   

Pyelonephritis associated with Staphylococcus intermedius in a Siberian tiger (Panthera tigris altaica)     

Jee H  Pakhrin B  Bae IH  Shin NS  Lee SI  Yoo HS  Kim DY 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(8):851-852

Pyelonephritis, in which Staphylococcus intermedius was isolated, was diagnosed in a 4-year-old female Siberian tiger (Panthera tigris altaica). At necropsy, the renal pelvis was dilated with a large amount of purulent exudates. Microscopically, the lesions of renal pelvis and medulla consisted of necrotic foci intermingled primarily with numerous degenerative neutrophils and a few lymphocytes, plasma cells and macrophages. Bacteriology showed the presence of S. intermedius. This is the first report on pyelonephritis associated with S. intermedius in wild felidae.  相似文献   

Hypercalcemia and high parathyroid hormone-related peptide concentration in a dog with a complex mammary carcinoma     

Bae BK  Kim CW  Choi US  Choi EW  Jee H  Kim DY  Lee CW 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(4):376-378

A 10-year-old female Dachshund was presented with a history of mammary masses, slight lethargy, polyuria, and polydipsia. Physical examination findings included masses involving the first, second, and fourth mammary glands of the left side. The mandibular, axillary, and right popliteal lymph nodes were mildly enlarged. Serum chemistry results included hypercalcemia (13.9 mg/dL, reference interval 8.0-11.5 mg/dL). Although intact parathyroid hormone (PTH) concentration (1.05 pmol/L) was below the reference interval (2-13 pmol/L), PTH-related protein (PTHrP) concentration was markedly increased (9.40 pmol/L, reference value < 2 pmol/L). The masses were surgically removed, and the histopathologic diagnosis was complex mammary carcinoma. Three weeks after surgery, serum total calcium concentration had decreased to 10.5 mg/dL. Resolution of the hypercalcemia and clinical signs supported a diagnosis of humoral hypercalcemia of malignancy associated with mammary gland carcinoma.  相似文献   

Overexpression of OCT4A ortholog elevates endogenous XIST in porcine parthenogenic blastocysts     

Jae Yeon HWANG  Kwang-Hwan CHOI  Dong-Kyung LEE  Seung-Hun KIM  Eun Bae KIM  Sang-Hwan HYUN  Chang-Kyu LEE 《The Journal of reproduction and development》2015,61(6):533-540

X-chromosome inactivation (XCI) is an epigenetic process that equalizes expression of X-borne genes between male and female eutherians. This process is observed in early eutherian embryo development in a species-specific manner. Until recently, various pluripotent factors have been suggested to regulate the process of XCI by repressing XIST expression, which is the master inducer for XCI. Recent insights into the process and its regulation have been restricted in mouse species despite the evolutionary diversity of the process and molecular mechanism among the species. OCT4A is one of the represented pluripotent factors, the gate-keeper for maintaining pluripotency, and an XIST repressor. Therefore, in here, we examined the relation between OCT4A and X-linked genes in porcine preimplantation embryos. Three X-linked genes, XIST, LOC102165544, and RLIM, were selected in present study because their orthologues have been known to regulate XCI in mice. Expression levels of OCT4A were positively correlated with XIST and LOC102165544 in female blastocysts. Furthermore, overexpression of exogenous human OCT4A in cleaved parthenotes generated blastocysts with increased XIST expression levels. However, increased XIST expression was not observed when exogenous OCT4A was obtained from early blastocysts. These results suggest the possibility that OCT4A would be directly or indirectly involved in XIST expression in earlier stage porcine embryos rather than blastocysts.  相似文献   

Employment of hordein subunit polymorphisms in establishing selection criteria for high quality malting barley (Hordeum vulgare L.)     

Yong Jin Lee  Tong Geon Lee  Woong Bae Jeon  Dae Yeon Kim  Min Jeong Hong  Man Bo Lee  Jong Nae Hyun  Mi Jung Kim  Mi Ja Lee  Cheol Su Park  Yong Weon Seo 《Journal of Crop Science and Biotechnology》2010,13(2):91-97

Cereal seed storage proteins are encoded by complex multigene families and their subunit profiles are highly related to end-use qualities. Each fraction of albumin and hordein was extracted and its subunit profile was evaluated in related to malt and grain quality parameters. The purpose of this study was to provide selection criteria for high quality malting barley using grain and malt quality parameters and biochemical-genetic information. Grain and malt quality of 13 local adaptability test (LAT) lines were evaluated for malting process. A total of 16 germplasm accessions of high or low seed storage protein content were also evaluated for biochemicalgenetic analysis. The correlation coefficients among quality parameters were analyzed. Several important quality parameters in brewing process showed significant positive or negative correlations. Seed storage protein subunits of albumin and hordein of all tested lines and accessions were evaluated using 12% 1D SDS-PAGE. Scored data of protein subunit’s presence or absence was applied to Agglomerative Hierarchical Clustering (AHC) for statistical analysis and showed specific grouping patterns among tested lines. Clustered lines with subunit information were highly related with agricultural performance and grain and malt qualities. Based on the profiles of seed storage protein subunits, association of hordein subunit of 38, 43, and 65 kDa with high malt scored lines was found. The obtained results would provide improved selection criteria for high quality malting barley in the malting barley breeding program.  相似文献   

浅谈南昌市种植业结构调整发展方向     

曹闽苏 《江西农业大学学报》1999,21(1):141-143

认为南昌市种植业结构调整发展方向应以市场为导向,继续抓好粮食生产,采取调整早稻面积,进行改种改制,调整早稻品种结构,进行收购办法改革。应采取４项配套措施：（１）增加市场观念,加快市场体系建设;（２）大力推广农业先进实用技术;（３）健全 完善社会化服务体系;（４）加强宏观调控,推出配套政策。  相似文献   

Microscopic observation of wood-based composites exposed to fungal deterioration     

Woo-Yang Chung  Seung-Gon Wi  Hyeun-Jong Bae  Byung-Dae Park 《Journal of Wood Science》1999,45(1):64-68

This study was conducted to investigate the susceptibility of various wood composite panels exposed to wood-deteriorating fungi. Five wood-attacking fungi (three mold fungi, one brown rot fungus, one white rot fungus) were inoculated into four types of commercial wood composite panels (plywood, oriented strand board, particleboard, and medium-density fiberboard). One solid wood sample was included as a control. The attacking patterns of the fungi in each panel was observed by scanning electron microscopy. The weight losses due to the exposure were compared. All wood composites were more or less susceptible to all fungi inoculated. The attacking mode of the fungi was highly dependent on the types of wood composite, which had inherently different shapes of voids owing to different shapes and characteristics of the raw furnish materials used. Plywood and medium-density fiberboard showed a large weight loss after an 8-week exposure to decay fungi. Plywood is the most susceptible to white and brown rot fungi. This study indicates that all wood composite panels should undergo careful consideration to prevent fungal deterioration when they are used for exterior and humid interior applications.  相似文献