Growth performance and color enhancement of goldfish,Carassius auratus,fed diets containing natural dyes extracted from annatto (Bixa orellana) seeds     

S.H.S Dananjaya  Prabuddha Manjula  A. S. Dissanayake  M. Edussuriya  K. Radampola  Bae Keun Park 《Journal Of Applied Aquaculture》2020,32(1):53-69

ABSTRACT

The present study was conducted to evaluate growth performance and color enhancement of goldfish, Carassius auratus, fed diets containing 0, 50, 100, 200, and 250 mg kg^?1 diet of annatto dye (AD) for 60 days. The survival rate was significantly higher in fish fed 100, 200, and 250 mg AD kg^?1 diet over than these fed control and 50 mg AD kg^?1 diet (p < 0.05). AD significantly (p <0 .05) increased the pigmentation in the skin and caudal fin of goldfish in a concentration dependent manner (R² = 0.995, 0.997). The highest amount of total carotenoid deposition in fish skin and fins were given by diets containing 200–250 mg AD kg^?1 diet. The highest redness (a*) of 43.21 and yellowness (b*) of 12.53 were obtained by 250 and 50 mg AD kg^?1, respectively. The present results show that AD can be successfully used as an alternative natural carotenoid source in goldfish diets at levels of 200–250 mg AD kg^?1 diet.  相似文献   

Bacterial shoot blight of sweet crab apple caused by Pseudomonas viridiflava     

Okhee Choi  Yeyeong Lee  Byeongsam Kang  Seunghoe Kim  Juyoung Bae  Jinwoo Kim 《Forest Pathology》2020,50(4)

Sweet crab apple (Malus coronaria) is a popular ornamental tree planted in gardens and urban forests in South Korea. In 2018, severe symptoms were observed on street plantings of sweet crab apple in Jinju, South Korea including shoot blight: shoot tips, twigs and foliage of infected trees turned brown, and dried completely. The causal agent isolated from the disease lesions was identified as Pseudomonas viridiflava based on biochemical tests, pathogenicity, and 16S rRNA and rpoD gene sequencing. On artificial inoculation, this pathogen not only caused shoot blight on sweet crab apple, but also bulb rot and leaf blight on onion. Here, we report the occurrence of bacterial shoot blight caused by P. viridiflava in sweet crab apple for the first time in the world.  相似文献   

Ligiamycins A and B,Decalin-Amino-Maleimides from the Co-Culture of Streptomyces sp. and Achromobacter sp. Isolated from the Marine Wharf Roach,Ligia exotica     

Hyung-Ju Lim  Joon Soo An  Eun Seo Bae  Eunji Cho  Sunghoon Hwang  Sang-Jip Nam  Ki-Bong Oh  Sang Kook Lee  Dong-Chan Oh 《Marine drugs》2022,20(2)

Streptomyces sp. GET02.ST and Achromobacter sp. GET02.AC were isolated together from the gut of the wharf roach, Ligia exotica, inhabiting the intertidal zone of the west coast of Korea. The co-cultivation of these two strains significantly induced the production of two new metabolites, ligiamycins A (1) and B (2), which were barely detected in the single culture of Streptomyces sp. GET02.ST. The planar structures of ligiamycins A (1) and B (2) were elucidated as new decalins coupled with amino-maleimides by the analysis of various spectroscopic data, including nuclear magnetic resonance (NMR), ultraviolet (UV), and mass (MS) data. The assignment of two nitrogen atoms in amino-maleimide in 1 was accomplished based on ¹H-¹⁵N heteroatom single quantum coherence spectroscopy (HSQC) NMR experiments. The relative configurations of the ligiamycins were determined using rotating frame Overhauser effect spectroscopy (ROESY) NMR data, and their absolute configurations were deduced by comparing their experimental and calculated optical rotations. Ligiamycin A (1) displayed antibacterial effects against Staphylococcus aureus and Salmonella enterica, while ligiamycin B (2) exhibited mild cell cytotoxicity against human colorectal cancer cells.  相似文献