Molecular characterization of lipoxygenase genes and their expression analysis against biotic and abiotic stresses in Panax ginseng     

Kwi-Sik Bae  Shadi Rahimi  Yu-Jin Kim  Balusamy Sri Renuka Devi  Altanzul Khorolragchaa  Johan Sukweenadhi  Jeniffer Silva  Davaajargal Myagmarjav  Deok-Chun Yang 《European journal of plant pathology / European Foundation for Plant Pathology》2016,145(2):331-343

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Mass production of rubusoside using a novel stevioside-specific β-glucosidase from Aspergillus aculeatus     

Ko JA  Kim YM  Ryu YB  Jeong HJ  Park TS  Park SJ  Wee YJ  Kim JS  Kim D  Lee WS 《Journal of agricultural and food chemistry》2012,60(24):6210-6216

Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific β-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50-60 °C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by (1)H and (13)C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other β-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 μL of SSGase at pH 5.1 and 63 °C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries.  相似文献   

Influenza virus neuraminidase inhibitory activity of phlorotannins from the edible brown alga Ecklonia cava     

Ryu YB  Jeong HJ  Yoon SY  Park JY  Kim YM  Park SJ  Rho MC  Kim SJ  Lee WS 《Journal of agricultural and food chemistry》2011,59(12):6467-6473

Influenza A virus infections continue to pose a major threat to humans and several animal species. Neuraminidase (NA) is one of the most promising targets for the development of drugs against influenza viruses because of its critical role in the viral life cycle. During the course of a search for NA inhibitors from edible natural sources, we found that the ethyl acetate layer of ethanol extracts of Ecklonia cava showed extremely high NA-inhibitory activity (72.1% inhibition at 30 μg/mL). Bioactivity-guided fractionation of the ethyl acetate layer yielded five phlorotannins, identified as phloroglucinol (1), eckol (2), 7-phloroeckol (3), phlorofucofuroeckol (4), and dieckol (5). The inhibitory activities of these compounds (1-5) against NAs from group-1 (A/Bervig_Mission/1/18 [H1N1], A/PR/8/34 [H1N1]) and group-2 (A/Hong Kong/8/68 [H3N2], A/Chicken/Korea/MS96/96 [H9N2]) influenza A were evaluated to determine potencies and kinetic behavior. Analyses using various in vitro influenza A virus NA assays showed that all five phlorotannin derivatives were selective NA inhibitors. Of the phlorotannins, phlorofucofuroeckol (4) exhibited the most potent inhibitory activities toward group-1 NAs (IC?? values, 4.5 and 14.7 μM), whereas dieckol (5) potently inhibited group-2 NAs. Kinetic analyses indicated that compounds 1-5 were all noncompetitive. Notably, these noncompetitive inhibitors synergized with oseltamivir to enhance the NA-inhibitory effects of oseltamivir.  相似文献   

Detection and characterization of bovine-like coronaviruses from four species of zoo ruminants     

Chung JY  Kim HR  Bae YC  Lee OS  Oem JK 《Veterinary microbiology》2011,148(2-4):396-401

Five coronaviruses (CoVs) were detected in diarrheal feces from four zoo ruminant species: one wisent (Bison bonasus), two Himalayan tahr (Hemitragus jemlahicus), one sitatunga (Tragelaphus spekii), and one nyala (Tragelaphus angasii). We sequenced and analyzed the spike (S) and hemagglutinin/esterase (HE) genes of these viruses and compared the nucleotide (nt) and deduced amino acid (aa) sequences with those of other bovine CoV (BcoV) strains. Comparison of the entire deduced aa sequences of the S and HE glycoproteins revealed no specific differences that would account for discrimination between bovine-like CoV strains from zoo ruminants and BcoVs strains. In addition, the 99.9% aa identity among the five CoV strains revealed that the ruminants were infected by the same strain. Phylogenetically, bovine-like CoVs belong to group 2a CoVs, which are related most closely to the BcoV strains recently isolated in Korea. These data suggest that cattle are potential reservoirs for CoVs that are capable of infecting zoo ruminants.  相似文献   

Dyeing of cotton and polyester/cotton blend with disperse dyes using sodium 2-(2,3-dibromopropionylamino)-5-(4,6-dichloro-1,3,5-triazinylamino)-benzenesulfonate     

Mikyung Kim  Seokhan Yoon  Taekyeong Kim  Jin-seok Bae  Namsik Yoon 《Fibers and Polymers》2006,7(4):352-357

The cotton fabrics were pretreated by sodium 2-(2,3-dibromopropionylamino)-5-(4,6-dichloro-1,3,5-triazinylamino)-benzenesulfonate (DBDCBS) at alkaline condition of room temperature and then dyed with four disperse dyes having amino groups (C.I. Disperse Yellow 9, C.I. Disperse Red 11, C.I. Disperse Blue 56 and C.I. Disperse Violet 1) at acidic condition of high temperature. A novel hetero-bifunctional bridge compound,DBDCBS, has two reactive groups such as dichloro-s-triazinyl group andα, β-dibromopropionylamido group. The first has reactivity towards hydroxy group of cellulosic fiber and the second shows reactivity towards amino groups of disperse dye containing amino groups. The results indicate that it is possible to dye polyester/cotton blend at one-bath dyeing using one kind of disperse dye containing amino groups. Therefore, two kinds of dyeing methods such as two-bath process one-bath dyeing and one-bath process one-bath dyeing were investigated and their dyeabilities were compared. The differences between these two methods were negligibly small so that perfect one-bath one-step dyeing of polyester/cotton blend by one kind of disperse dye was achieved.  相似文献   

Evaluation of new metallized direct dyes for mutagenicity using the Salmonella mammalian mutagenicity assay     

Jin-Seok Bae  Harold S. Freeman 《Fibers and Polymers》2005,6(3):235-243

A series of new metallized direct dyes based on benzidine congeners, 2,2′-dimethyl-5,5′-dipropoxybenzidine and 5,5′-dipropoxybenzidine, were evaluated for mutagenicity in Salmonella typhimurium strains TA98 and TA100. All of the dyes examined were judged to be non-mutagenic with and without metabolic activation while toxicity was seen in some dyes at high doses. The study also suggested that the standard Salmonella mutagenicity plate-incorporated assay was an excellent method for evaluation of dyes for mutagenicity.  相似文献   

Antiproliferative Activity of Korean Wild Vegetables on Different Human Tumor Cell Lines     

Buk-Gu Heo  Sang-Uk Chon  Yun-Jum Park  Jong-Hyang Bae  Su-Min Park  Yong-Seo Park  Hong-Gi Jang  Shela Gorinstein 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(4):257-263

This study was conducted to determine the antiproliferative activity of 24 Korean wild vegetables. The methanol extracts of these wild vegetables were used against lung, breast, colon and gastric cancer cells, and the results were assessed by MTT assay. It was found that at the extract concentration of 400 mgL⁻¹ 14 plants exercised antiproliferative activity over 80% against the lung cancer cells, one plant among six—against breast cancer cells, and two plants among six—against colon cancer cells, respectively. Eighteen wild vegetables had the hyperplasia inhibition activity against gastric cancer cells over 23.6% at all extract concentrations, however, only six plants had the antiproliferative activity over 80% in 600 mgL⁻¹. It was found that the extracts from Youngia sonchifolia, Synurus deltoides, Syneilesis palmata, and Cephalonoplos segetum, in concentration of 400 mgL⁻¹ inhibited the hyperplasia of lung cancer cells over 95% and Angelica gigas—both lung and colon cancer cells over 95%. In conclusion, the studied wild vegetables’ methanol extracts possess dose dependent antiproliferative properties, based on their bioactive compounds, mainly polyphenols, but some of them as Hypericum ascyron against lung cancer are not effective and even course harm.  相似文献   

Application of the rpoS gene for the detection of Vibrio anguillarum in flounder and prawn by polymerase chain reaction     

Kim DG  Bae JY  Hong GE  Min MK  Kim JK  Kong IS 《Journal of fish diseases》2008,31(9):639-647

Vibrio anguillarum , an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum . PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum . Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA , or from six colony-forming units (CFU) mL⁻¹ of cultured V. anguillarum . However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g⁻¹ tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.  相似文献   

Time-dependent low-field MRI characteristics of canine blood: an in vitro study     

Jimo Jeong  Sangjun Park  Eunseok Jeong  Namsoo Kim  Minsu Kim  Yechan Jung  Youngkwon Cho  Kichang Lee 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(1):103-109

This study was conducted to assess time-sensitive magnetic resonance (MR) changes in canine blood using low-field MR. Arterial and venous blood samples were collected from eight healthy beagle dogs. Samples were placed in 5-mL tubes and imaged within 3 hours of collection at 1 day intervals from day 1 to day 30. The following sequences were used: T1-weighted (T1W), T2-weighted (T2W), fluid-attenuated inversion recovery (FLAIR), short tau inversion recovery (STIR), and T2-star gradient-echo (T2^*-GRE). Visual comparison of the images revealed that four relatively homogenous blood clots and twelve heterogeneous blood clots developed. The margination of the clot and plasma changed significantly on day 2 and day 13. On day 2, heterogeneous blood clots were differentiated into 2 to 3 signal layers in the T2W, T1W, and especially the STIR images. Hypointense signal layers were also detected in the blood clots in STIR images, which have T2 hypo, FLAIR hypo, and T1 hyper intense signals. In all images, these signal layers remained relatively unchanged until day 13. Overall, the results suggest that hematomas are complex on low-field MRI. Accordingly, it may not be feasible to accurately characterize hemorrhages and predict clot age based on low-field MRI.  相似文献   

Transgenic rice plants carrying RNA interference constructs of AOS (allene oxide synthase) genes show severe male sterility     

H. K. Bae  H.‐G. Kang  G.‐J. Kim  H.‐J. Eu  S.‐A. Oh  J. T. Song  I.‐K. Chung  M.‐Y. Eun  S. K. Park 《Plant Breeding》2010,129(6):647-651

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