Evidence of Genetic Exchange by Parasexual Recombination and Genetic Analysis of Pathogenicity and Mating Type of Parasexual Recombinants in Rice Blast Fungus, Magnaporthe oryzae     

Noguchi MT  Yasuda N  Fujita Y 《Phytopathology》2006,96(7):746-750

ABSTRACT A selectable marker gene conferring resistance to bialaphos (BI) was introduced into rice blast isolate Y90-71BI and another conferring resistance to blasticidin S (BS) into isolate 3514-R-2BS of Magnaporthe oryzae to demonstrate exchange of DNA. Colonies obtained from co-cultures of these two isolates were resistant to both BI and BS and had both resistance genes as shown by Southern blot analysis of their genomic DNA. Conidia from these BI-BS-resistant isolates had only one nucleus per cell after staining with 4',6-diamidino-2-phenylindole (DAPI). Using flow cytometry, however, these BI-BS-resistant isolates were found to be haploid. Segregation of BI-BS-resistant isolates for pathogenicity (avirulence to virulence) on rice line K59-1 was consistent with a 1:1 ratio, as was segregation for mating type. These BI-BS-resistant isolates were thus apparently derived from parasexual exchange of DNA and the segregation of pathogenicity and of mating type of the parasexual recombinants might correspond to that of the progeny of the offspring of the sexual cross.  相似文献   

Preparation of strongly acidic cation-exchange resins from gymnosperm acid hydrolysis lignin   总被引：1，自引：0，他引：1  

Seiichi Yasuda  Kyoko Asano 《Journal of Wood Science》2000,46(6):477-479

The chemical preparation of strongly acidic cation-exchange resin from sulfuric acid lignin (Klason lignin) (SAL), a typical acid hydrolysis lignin, was investigated. Sulfonation of resinified SAL itself gave a resin with an ion-exchange capacity of 2.3 mEq/g. After resinification with formaldehyde, the phenolized SAL with a reactivep-hydroxyphenyl group yielded a resin with an ion-exchange capacity of 3.2 mEq/g. The latter capacity is superior to that of the corresponding commercial phenol-type resins (2–3 mEq/g), but did not reach the level of the corresponding commercial styrene-type resins (4-5 mEq/g).This paper was presented at the 43th Lignin Symposium, Fuchu, October 1998  相似文献   

Improvement in recruitment of Japanese sardine with delays of the spring phytoplankton bloom in the Sea of Japan          下载免费PDF全文

Taketoshi Kodama  Taku Wagawa  Seiji Ohshimo  Haruyuki Morimoto  Naoki Iguchi  Ken‐Ichi Fukudome  Tsuneo Goto  Motomitsu Takahashi  Tohya Yasuda 《Fisheries Oceanography》2018,27(4):289-301

To evaluate the impact of temporal variation of primary productivity on the recruitment of Japanese sardine (Sardinops melanostictus) in the Sea of Japan, the phenology of sea surface phytoplankton abundance was estimated from 8 day multiple satellite (SeaWiFS, MODIS‐Aqua, MERIS, and VIIRS) derived sea surface chlorophyll (SSChl) a concentrations from January 1998 to December 2015. Because relationships between SSChl a and in situ chlorophyll a concentrations were significantly different among periods based on the satellite combinations used, maximum and minimum SSChl a concentrations of 1 year were relativized as 1 and 0, respectively. Spatio‐temporal variation of relativized SSChl a concentrations was determined by using empirical orthogonal function (EOF) analysis. Scores in the first EOF mode denoted the basin‐scale variations of SSChl a concentrations in the Sea of Japan, and the major peak from the end of February to the end of May displayed the spring bloom. The logarithm of recruitment per spawner (LNRPS) for sardine was positively affected by delays in the start and end dates of the spring phytoplankton bloom. The delay of the date of the lowest sea surface temperature contributed to the delay of the end of the spring bloom during the period 1998–2015 and elevated the LNRPS during the period 1982–2015. Sardine spawns in the southern Sea of Japan from April to May, hence, delays of the spring bloom prolonged its overlap with sardine larval periods, and thus improved the recruitment of Japanese sardine in the Sea of Japan.  相似文献   

Inhibition of turkey herpesvirus replication by anti-cellular serum     

T Mikami  T Higashihara  M Yasuda  K Kunihiro  H Kodama  H Izawa  I Okada 《Avian diseases》1985,29(1):250-255

Quail embryo fibroblasts were used to investigate how rabbit and chicken antisera against chicken erythrocytes carrying different B alleles of the major histocompatibility antigens affect the neutralization of herpesvirus of turkeys (HVT). Although the neutralizing activities of these antisera were rather low, the HVT propagated in chicken embryo fibroblasts (CEF) from certain genotypic embryos was neutralized more by the antisera to the corresponding erythrocytes. After absorption of these antisera with homologous erythrocytes, the neutralizing activity of the absorbed sera was reduced slightly. These results reveal that the virion antigens of HVT might be partially associated with the host cell antigens of the fibroblast infected with the virus. The virus grown in these cells might incorporate the host cell antigens, including histocompatibility antigens, into the virion envelope.  相似文献   

Analysis of tissue creatine phosphokinase isoenzymes by histoelectrophoresis     

J Yasuda  B Syuto  K Too 《The Japanese journal of veterinary research》1985,33(1-2):19-25

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Scanning electron microscopy of Theileria sergenti   总被引：1，自引：0，他引：1  

S Higuchi  S Kawamura  Y Yasuda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1985,47(1):133-137

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Development of Babesia gibsoni in the gut epithelium of the tick, Haemaphysalis longicornis.     

S Higuchi  S Simomura  H Yoshida  F Hoshi  S Kawamura  Y Yasuda 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(1):129-131

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A case of feline large granular lymphoma     

R Goitsuka  M Tsuji  Y Matsumoto  C Onda  K Matsuoka  K Yokomori  K Yasuda  Y Hirota  K Ono  T Hayashi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(2):593-595

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Pathology of genital amoebiasis in a female dog     

A Yasuda  K Midoro  H Nakayama  R Takahashi  N Goto  K Fujiwara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(2):549-551

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Serum total amylase activity and its isoenzyme fractions in newborn pigs with relation to growth and iron dextran-treatment.     

M Akuzawa  M Morizono  K Sudo  N Yasuda  K Okamoto  E Deguchi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(6):1151-1155

Serum amylase activity was analyzed chromogenically (blue starch method) and its isoenzyme was separated by cellulose acetate membrane electrophoresis in newborn pigs comparing with those in adult pigs. Serum amylase activity in newborn pigs was about one half of adults' and increased with age. Serum amylase isoenzymes were separated into 4 fractions from the cathode. The isoenzymes were also classified in 5 types on the basis of the combination of these 4 fractions. Incidences of the fractions and the types were not significantly different between adults and newborns. After the iron dextran treatment, serum amylase activity as well as levels of erythrocytes, hemoglobin and packed cell volume increased. The rates of increase in hemoglobin and packed cell volume were more significant in the double treatment group than in the single treatment group, while serum amylase activity increased similarly in these 2 groups. The increase in serum amylase activity seemed to indicate that the development of amylase-producing organs was reinforced by the iron compound treatment. It is thought that serum amylase activity may be an indicator of healthy growth in newborn pigs.  相似文献