Porcine salmonellosis. 3. Production of fibrinous colitis by intravenous injections of a mixture of viable cells of salmonella cholerae-suis and disintegrated cells of the same agent, or hemolytic Escherichia coli     

Knut Nordstoga  Morten Fjlstad 《Acta veterinaria Scandinavica》1970,11(3):380-389

Hemorrhagic necrosis of the gastric and colonic mucosa with capillary thrombosis were induced in pigs experimentally infected with Salmonella cholerae-suis when an additional intravenous supply of disintegrated cells of Salmonella cholerae suis or hemolytic Escherichia coli was administered. Except for the gastrointestinal lesions, the necropsy findings were almost identical with the picture found in acute septicemic salmonellosis. The gastrointestinal changes were interpreted as a special gastroenteric effect of endotoxins of Gramnegative bacteria during the development of septicemic salmonellosis.  相似文献   

Streptococcus equi subsp. zooepidemicus isolates from equine infectious endometritis belong to a distinct genetic group     

Camilla Dooleweerdt Rasmussen  Maria Mathilde Haugaard  Morten Roenn Petersen  Jesper M?ller Nielsen  Hanne Gervi Pedersen  Anders Miki Bojesen 《Veterinary research》2013,44(1):26

Streptococcus equi subsp. zooepidemicus is the pathogen most commonly isolated from the uterus of mares. S. zooepidemicus is an opportunistic pathogen and part of the resident flora in the caudal reproductive tract. The aim of this study was to investigate whether a genotypically distinct subpopulation of S. zooepidemicus is associated with endometritis in the mare, by genotyping and comparing uterine S. zooepidemicus strains with isolates from the vagina and clitoral fossa. Mares with (n = 18) or without (n = 11) clinical symptoms of endometritis were included. Uterine samples were obtained using a guarded endometrial biopsy punch, whereas a swab was used to recover samples from the cranial vagina and the clitoral fossa. If S. zooepidemicus was present, up to three colonies were selected from each anatomical location (max. 9 isolates per mare). Bacterial isolates were characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). S. zooepidemicus was isolated from the endometrium of 12 mares. A total of 88 isolates were analyzed by PFGE: 31 from the endometrium, 26 from the cranial vagina and 31 isolates from the clitoral fossa. For MLST 21 isolates were chosen. Results demonstrated a higher genetic similarity of the isolates obtained from infectious endometritis compared to isolates obtained from the caudal reproductive tract. In conclusion, we demonstrate for the first time that a genetically distinct group of S. zooepidemicus is associated with infectious endometritis in the mare.  相似文献   

Evidence of cormorant‐induced mortality,disparate migration strategies and repeatable circadian rhythm in the endangered North Sea houting (Coregonus oxyrinchus): A telemetry study mapping the postspawning migration          下载免费PDF全文

Lasse Fast Jensen  Paul Rognon  Kim Aarestrup  Jesper Wøhlk Bøttcher  Cino Pertoldi  Søren Nøhr Thomsen  Morten Hertz  Jacob Winde  Jon C. Svendsen 《Ecology of Freshwater Fish》2018,27(3):672-685

Life history theory predicts a trade‐off between migration and residency where migration is favoured when it infers elevated fitness. Although migration to more favourable environments may offer higher growth rates, migrants often experience increased mortality due to predation. Here, we investigated mortality and migration behaviour of the North Sea houting (Coregonus oxyrinchus), an anadromous salmonid endemic to the Wadden Sea. We used acoustic telemetry to map the migration of the only remaining indigenous population by applying stationary hydrophones combined with manual tracking. Data suggested a total mortality of 26%, with 30% of the total mortality attributed to predation by great cormorants (Phalacrocorax carbo sinensis), highlighting that North Sea houting conservation could be jeopardised by increased cormorant predation. Risk of cormorant predation was size‐dependent, with smaller fish suffering higher risk of predation. The study found North Sea houting to exhibit disparate migration strategies and identified a lentic area in the estuary as an important habitat. Two newly established artificial lakes within the river system significantly reduced the migration speeds, possibly indicating constrained navigation through the lakes. The migration into the Wadden Sea correlated with temperature perhaps indicating osmoregulatory constraints of sea entry. Unlike many salmonid species, migration occurred both day and night. Moreover, fish exhibited repeatable individual differences in diel activity patterns, suggesting that individuals differ consistently in their migratory activity throughout the 24‐hr period. Our study provides novel information on salmonid migration, which is crucial for the development of science‐based conservation strategies.  相似文献   

Direct high-resolution genotyping of Toxoplasma gondii in arctic foxes (Vulpes lagopus) in the remote arctic Svalbard archipelago reveals widespread clonal Type II lineage     

Prestrud KW  Asbakk K  Mørk T  Fuglei E  Tryland M  Su C 《Veterinary parasitology》2008,158(1-2):121-128

Characterization of Toxoplasma gondii genotypes in hosts living in remote, isolated regions is important for elucidating the population structure and transmission mode of this parasite. Herein, we report the results of direct genotyping of T. gondii in brain tissue of arctic foxes (Vulpes lagopus) from the remote, virtually cat-free, high arctic islands of Svalbard. DNA extracts from brains of 167 seropositive arctic foxes (including four cases of fatal toxoplasmosis) and 11 seronegative arctic foxes were genotyped at 10 loci (SAG1, SAG2, SAG3, BTUB, GRA6, L358, c22-8, c29-2, PK1, and Apico) using the polymerase chain reaction-restriction fragment length polymorphism method. Of the 167 samples from seropositive foxes (including toxoplasmosis cases), 31 were genotyped at all 10 loci and 24 were genotyped at four to nine loci. To ensure confidence in T. gondii strain genotyping, samples for which less than four loci were genotyped were not considered positive. None of the 11 samples from seronegative foxes was positive for the 10 markers. Of the 55 samples that genotyped positively, 46 were of the Type II strain, 7 were of the Type III strain, and 2 were of atypical T. gondii strains. Five representative samples of the three genotypes were sequenced at loci SAG2, SAG3, GRA6, PK1, and UPRT-1. The DNA sequences confirmed the genotyping results. This study shows that the archetype Type II T. gondii strain, which is most widely distributed in North America and Europe, also predominates in arctic foxes on the Svalbard archipelago. This suggests that the T. gondii at this location originate from continental Europe and that transmission may be mediated by migrating birds. This study highlights the significance of long-distance transport of T. gondii and demonstrates that high-resolution genotyping protocols are useful for direct genetic studies of T. gondii when isolation of live parasites is infeasible.  相似文献   

Appetite,metabolism and growth regulation in Atlantic salmon (Salmo salar L.) exposed to hypoxia at elevated seawater temperature          下载免费PDF全文

Vibeke Vikeså  Leo Nankervis  Ernst Morten Hevrøy 《Aquaculture Research》2017,48(8):4086-4101

High temperature combined with low dissolved oxygen (DO) is one of the most challenging environmental conditions farmed fish experience; thus, understanding their impact on growth regulation is of relevance to cultured and wild populations. This study examines appetite‐ and growth‐regulating mechanisms in Atlantic salmon postsmolt exposed to either high (HO) or low oxygen (LO) at a suboptimally high temperature (17°C). Additionally, the effects of high (HE) and low (LE) dietary energy (DE) were examined. After a month of treatment, analyses of hormones, regulating appetite (ghrelin) and growth (growth hormone receptor ghr1 and insulin‐like growth factor IGF‐1), and free amino acids (FAA) were measured pre‐ and postprandially at ?4, ?2, 0, 2, 4 and 6 h. No preprandial ghrelin peaks were detected despite a significant reduction in feed intake and growth under hypoxia compared to normoxia. LO treatment also had an overall negative effect on survival compared to HO, while nutrient retention efficiency, FCR and plasma FAA concentrations were unaffected (P > 0.05). Feeding HE diet resulted in increased growth (+17%) and improved FCR (?14%) and energy retention efficiency (+26%) independent of DO. Plasma FAA concentrations were unaffected by LO treatment and DE (P > 0.05). Growth regulatory gene expressions possibly reflect an overall lower growth at a high temperature overriding the impacts of DO and DE. This study also indicates that optimal adaptation time to environmental conditions and feeding regime is crucial for establishing a regular hormonal appetite signalling that reflects real feeding anticipation in salmon.  相似文献   

Lysine limitation alters the storage pattern of protein,lipid and glycogen in on‐growing Atlantic salmon     

Raja Mansingh Rathore  Bjørn Liaset  Ernst Morten Hevrøy  Adel El‐Mowafi  Marit Espe 《Aquaculture Research》2010,41(11):e751-e759

This experiment aimed to test the interaction of lysine limitation with nutrient accretion and muscle carnitine depot in Atlantic salmon. Fish were fed adequate or low‐lysine diets for 3 months. Lysine intake was significantly less (48%) in fish fed the low‐lysine diet as compared with that fed the adequate one. There was no difference in dietary amino acids between treatments, with the exception of lysine. The lower lysine intake was reflected in plasma free lysine being 52% less while the free lysine concentration in the liver and muscle were unaffected. Although there was no significant difference between voluntary feed intakes among treatments, fish fed the low‐lysine diet had reduced growth, protein and energy deposition as compared with fish fed the adequate lysine diet. White trunk muscle contained more glycogen and less protein in fish fed the low‐lysine diet while no difference in lipid was observed. The livers from fish fed the low‐lysine diet contained less glycogen and slightly more fat and protein than the livers from fish fed the adequate lysine diet. Lysine limitation reduced carnitine in the liver without affecting muscle carnitine depot. Thus, low‐lysine diets did not likely affect the fatty acid oxidation capacity. This fact was supported by unaffected fatty acid profiles and lipid classes between treatments during the 3‐month study. In conclusion, lysine limitation does not deplete the muscle carnitine depot during the on‐growing seawater phase of Atlantic salmon, but affects the deposition pattern of nutrients.  相似文献   

Publish or Die     

Morten Sivertsvik 《Journal Of Aquatic Food Product Technology》2019,28(5)

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Prediction of Starch,Soluble Sugars and Amino Acids in Potatoes (<Emphasis Type="Italic">Solanum tuberosum</Emphasis> L.) Using Hyperspectral Imaging,Dielectric and LF-NMR Methodologies     

Anders Kjær  Glenn Nielsen  Søren Stærke  Morten Rahr Clausen  Merete Edelenbos  Bjarke Jørgensen 《Potato Research》2016,59(4):357-374

Handling and processing of potatoes is performed in increasingly large and more automated facilities, and the industry calls for more automated machinery for quality assessment and sorting by concentration of starch, soluble sugars, protein, amino acids etc. of the potato tubers. The present study was designed to evaluate five different scanning methods for their potential use in potato assessment and sorting. Two methods were based on hyperspectral imaging, two were based on dielectric/bio-impedance and one was based on low-field nuclear magnetic resonance. A set of 60 potatoes of 10 different cultivars were simultaneously sampled for analyses of content and scanned by the five different scanning methods. The resulting multivariate dataset was used to estimate the prediction ability of the individual scanning methods on starch-related parameters, selected simple sugars, selected amino acids, conductivity of pressed cell sap and cell sizes. Results showed that most types of spectral analyses had relatively high potential for predicting the starch-related parameters and medium potential for predicting the concentration of the reducing sugars fructose and glucose. Most methods showed medium potential for prediction of several amino acids, including asparagine, which showed particularly promising predictions in the hyperspectral analyses of intact potatoes. The presented screening study enabled us to perform robust choices for the further development and optimization of the methods and instruments for industrial implementation.  相似文献   

Antifungal effect of chito-oligosaccharides with different degrees of polymerization     

Md. Hafizur Rahman  Linda Gordon Hjeljord  Berit B. Aam  Morten Sørlie  Arne Tronsmo 《European journal of plant pathology / European Foundation for Plant Pathology》2015,141(1):147-158

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Predator-prey relationships and responses of ungulates and their predators to the establishment of protected areas: A case study of tigers, leopards and their prey in Bardia National Park, Nepal     

Per Wegge  Morten Odden 《Biological conservation》2009,142(1):189-202

Interactions among sympatric large predators and their prey and how they respond to conservation measures are poorly known. This study examines predictions concerning the effects of establishing a protected area in Nepal on tigers (Panthera tigris), leopards (Panthera pardus), and their ungulate prey. Within a part of the park, after 22 years the total density of wild ungulates had increased fourfold, to ca. 200 animals/km², almost exclusively due to a remarkable increase in chital deer (Axis axis). Tiger density also increased markedly to nearly 20 animals/100 km², whereas leopard density did not and was ca. 5 animals/100 km². The prediction that grazers should increase more than browsers was only partially supported. The prediction of positive density dependence in prey selection was not supported. Instead, the most abundant species (chital and hog deer, Axis porcinus) were killed less frequently than expected, whereas the lower-density wild boar (Sus scrofa) was preferred. Predictions that (i) initially rare species suffer highest predation was partially supported, that (ii) predation is highest among the most abundant prey was not supported, and that (iii) predation is highest among the most preferred prey independently of their densities was supported. Clearly, the conservation efforts adopted in Bardia were successful, as both tigers and their natural prey base increased. However, the positive numerical response of tigers limited and depressed the abundance of some prey species. Thus, conservation activities aimed at restoring large predators are likely to change in the composition of the overall mammal community, potentially eliminating rare but preferred prey species.  相似文献