3株烟草内生细菌促进作物生长作用的研究   总被引：3，自引：2，他引：1  

杨珍福  吴毅歆  聂兴成  周普雄  宋青朋  尚海丽  何月秋 《江西农业学报》2014,(11):58-60

通过漂浮育苗试验以及大田试验,研究了烟草内生细菌YN201408、YN201442和YN201448的促生长作用。结果表明:3个菌株的菌悬液均可促进烟草、番茄、白菜、玉米和油菜的生长,其中YN201448对烟草和番茄的促生长效果最好,YN201408促进白菜生长的作用较大,而YN201442对玉米和油菜的促生长作用最好。  相似文献   

两种土壤碱解氮测定方法的比较     

莎娜  张三粉  骆洪  狄彩霞  聂晶  李秀萍  连海飞 《内蒙古农业科技》2014,(6):25-26

测量土壤碱解氮含量过去一般使用碱解扩散吸收法,试验研究旨在使用德国GERHARD凯氏定氮蒸馏法测定土壤碱解氮,对凯氏定氮蒸馏法进行了改进,确定了合适的蒸馏时间和蒸馏效率,与传统碱解扩散法相比,测量结果更准确,紧密度更高,操作简便,节省时间,为土壤碱解氮含量测定提供了一种准确、快速、高效的测定方法。  相似文献   

家庭蔬菜病虫害非农药防治技术     

李俊萍  胡淑明  乜兰春 《现代农业科技》2014,(21)

近年来,随着人们生活水平的提高和对蔬菜消费观念的改变,利用庭院、阳台、露台、楼顶等家居空间开展家庭蔬菜种植悄然兴起。从品种的选择与搭配、基质选择、物理措施、植物制剂、肥料及家居日用品等方面介绍了家庭蔬菜病虫害的非农药防治技术,以期为家庭蔬菜安全生产提供参考。  相似文献   

关于中心打孔机的设计研究     

聂荣臻  曹建中 《湖南农机》2014,(10)

本实用新型涉及一种中心打孔机,包括机壳、电机座、电机导轨和两个压杆,所述机壳内具有推块,机壳的顶部具有螺母,螺杆的一端通过螺母穿过机壳的顶部与推块相连,电机导轨与机壳固定连接,电机座通过电机导轨与机壳滑动连接,电机座内具有电机,电机的输出端连接有动力头;所述推块的下端具有滚轮,推块的中部两侧对称开有向上的两个斜槽,每个压杆的一端装有导向轮,导向轮在斜槽内滑动,每个压杆的中部通过回转销与机壳转动连接,每个压杆的另一端也均装有滚轮。本实用新型的中心打孔机,不仅能快速的夹紧工件,三点快速夹紧,自动定心,实现自动打中心孔的目的。  相似文献   

鹫峰国家森林公园风景林健康评价研究     

马志林  陈丽华  韩鹏  于显威  聂森 《水土保持研究》2010,17(6)

健康的风景林可以持续发挥森林的生态效益和经济效益,可以让人类找回失去的自然风光.对风景林的健康评价是风景林健康管理的前提.结合特尔菲法(Delphi).在对鹫峰国家森林公园风景林实地调查和查阅相关林业年鉴、林业发展战略、林业规划的基础上,提出了鹫峰风景林健康评价指标体系,并依据该指标体系对鹫峰风景林近10 a以来(2000-2009年)和未来10 a以后(2009-2020年)的健康状况变化进行了分析.分析结果表明:鹫峰风景林的健康状况比10 a前有1.25倍的改善;在10 a以后鹫峰风景林的健康状况将进一步增强,提高1.24倍,提高幅度与前10 a基本相同;评价体系中表现最为活跃的指标是年龄结构、服务设施、火灾恢复力、社会效益综合指标、经济效益综合指标.  相似文献   

Characterization of MicroRNA* Species in Peking Duck Skin     

Li ZHANG  Xiu-juan XIE  Shan-gang JIA  Shu-dai LIN  Li-long AN  Wen LUO  Xin-zheng JIA  Qing-hua NIE  Xi-quan ZHANG 《农业科学学报》2013,12(9):1614-1619

A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3′-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the “seed region”. miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.  相似文献   

Role of type II innate lymphoid cells in browning of white adipose tissue     

SUN Jun  LIU Chao-bo  PAN Xiu-he  JIANG Wen-wen  LI Ming-cai  LI Yan 《园艺学报》2017,33(2):365

Type II innate lymphoid cells (ILC2s) are widely distributed in the blood, intestines, trachea, lung, spleen, liver, animal fat and skin, and involved in the innate immune responses. ILC2s have attracted much attention for its important roles in the conversion of white adipose to beige adipose. Studies have shown that ILC2s are essential for the proliferation and differentiation of adipocyte precursor cells, and they also play a vital role in anti-parasitic infection and allergic inflammation. This review discusses the discovery, differentiation, development, distribution and function of ILC2s, and their relationships with the browning of white adipose tissue for providing valuable references on understanding the pathogenesis, prevention and treatment of obesity and fat metabolism disorders.  相似文献   

矮丛蓝莓品种“美登”叶片转录组测序和生物信息学分析     

王云生  文光琴  聂飞 《北方园艺》2017,(1)

以矮丛蓝莓品种"美登"为试材,采用第二代测序技术对其嫩叶转录组进行了测序及生物信息学分析,研究了矮丛蓝莓功能基因组信息,以期增加对矮丛蓝莓生长发育分子机理的了解,并为矮丛蓝莓的分子标记辅助育种奠定基础。结果表明:在获得的约1G纯净数据,共拼装了41 929条Unigenes,其中被GO数据库成功注释Unigene分别涉及到生物学过程、细胞成分及分子功能相关的共45类生理代谢功能;KOG数据库注释到19 149条Unigenes,共涉及25条代谢通路;KEGG数据库成功注释13 072条Unigenes,共涉及到5个大类、18个中类、129条代谢通路;Nr数据库成功注释30 738条Unigenes,这些Unigenes共对应32 949条蛋白质的编码区。同时,生物信息学分析还显示,全部Unigenes中,有884条编码转录因子,涉及到55个家族;2 261条编码抗性基因,涉及18个家族;检测到6 591个SSR位点,其中2碱基重复的SSR数目最多,占总SSR位点数的70%。  相似文献   

“希姆劳特”葡萄在天津地区的引种表现及栽培技术     

黄建全  聂松青  温晓敏  路凤珍  田淑芬 《北方园艺》2017,(4)

经过连续6年的引种栽培区试发现,"希姆劳特"葡萄在天津地区具有极早熟、花芽分化好、品质优良、抗病性强等优良特性,可在天津地区适度发展该葡萄品种。现总结"希姆劳特"葡萄品种的引种区试情况及栽培技术要点。  相似文献   

Repression of SFRP5 promoter activity by hepatitis B virus X protein     

CHEN Lin-lin  XIE Qing  SHAN Xiao-liang  QUAN Hui-qin  CHEN Qing-mei  ZHOU Fan  NIE Dan  TANG Ni 《园艺学报》2013,29(2):200-204

AIM: To investigate the molecular mechanism that hepatitis B virus X protein (HBx) inhibits the promoter activity of secreted frizzled-related protein 5 (SFRP5). METHODS: Serial truncated fragments of SFRP5 promoter were amplified and cloned into luciferase reporter vector pGL3-Basic. LO2 cells were transfected with recombinants, and then infected with the adenoviral vector expressing HBx protein (Ad-HBx) or the analogous adenovirus expressing green fluorescent protein (Ad-GFP) for control. The infection efficiency was examined under a fluorescence microscope and the activity of SFRP5 promoter was measured by luciferase assay. RESULTS: Truncated fragments of SFRP5 promoter were successfully constructed. Compared with the pGL3-Basic control, the luciferase activity was 6.32±0.04 of the -478 bp~+47 bp fragment, 5.79±0.32 of the -811 bp~+47 bp fragment, 3.59±0.34 of the -1 235 bp~+47 bp fragment, 3.86±0.39 of the -1 677 bp~+47 bp fragment and 3.26±0.42 of the -2 072 bp~+47 bp fragment, respectively. Overexpression of HBx inhibited the activity of SFRP5 promoter. The average inhibitory rates were 44% for -478 bp~+47b, 46% for -811 bp~+47 bp, 28% for -1 235 bp~+47 bp, 24% for -1 677 bp~+47 bp and 40% for -2 072 bp~+47 bp, respectively. CONCLUSION: HBx suppresses the activity of SFRP5 promoter, leading to down-regulation of SFRP5 expression.  相似文献