Apospory in Sorghum bicolor (L.) Moench     

Hanna WW  Schertz KF  Bashaw EC 《Science (New York, N.Y.)》1970,170(3955):338-339

A line of Sorghum bicolor (L.) Moench was discovered to reproduce by apospory, a type of apomixis. The formation of an embryo by a nucellar cell without fertilization was establshed by cytological observations of ovaries and by progeny tests.  相似文献   

Determination of diastereomeric purity of tranylcypromine sulfate by proton magnetic resonance spectroscopy with lanthanide shift reagent     

G M Hanna  C A Lau-Cam 《Journal of the Association of Official Analytical Chemists》1989,72(4):556-558

Use of proton nuclear magnetic resonance spectroscopy and the lanthanide shift reagent, Eu(hfc)3, resulted in a simple and reliable method for determination of the diastereomeric purity of trans-2-phenylcyclopropylamine sulfate (tranylcypromine sulfate). Eu(hfc)3 effectively simplified the complex overlapping pattern of the 5 non-equivalent phenyl protons to a virtually first order pattern. At a shift reagent to substrate molar ratio of 0.75, and a substrate concentration of 0.25M (as the free base) in CDCl3, the resonances for the cis- and trans-ortho-phenyl protons at 11.39 and 9.99 ppm, respectively, were sufficiently resolved to be of use in quantitative work. Analysis of synthetic diastereomeric mixtures of 2-phenylcyclopropylamine sulfate by the proposed method yielded results that were in close agreement with the expected values. The mean recovery (SD) of trans-diastereomer was 100.51% (0.40) (n = 6). Levels of less than 2% of cis-diastereomer were measurable in cis-trans mixtures.  相似文献   

Preparation and stability of animal feed mixtures dosed with rotenone     

D A Kline  G R Hanna  C B Honaker  G O Kuhn  C W Jameson 《Journal of the Association of Official Analytical Chemists》1986,69(4):660-663

Studies at Midwest Research Institute for the National Toxicology Program show that rotenone/animal feed mixtures prepared by dry-mixing are more stable than mixtures produced by dosing the feed with alcoholic solutions of rotenone and then stripping the solvent. Also, recoveries of rotenone from the dry mix feeds are higher than those from feeds dosed by the solution method. A simplified analytical method from one previously reported for rotenone in feed is described.  相似文献   

Fresh Israeli Jaffa blond (Shamouti) orange and Israeli Jaffa red Star Ruby (Sunrise) grapefruit juices affect plasma lipid metabolism and antioxidant capacity in rats fed added cholesterol   总被引：2，自引：0，他引：2  

Gorinstein S  Leontowicz H  Leontowicz M  Krzeminski R  Gralak M  Martin-Belloso O  Delgado-Licon E  Haruenkit R  Katrich E  Park YS  Jung ST  Trakhtenberg S 《Journal of agricultural and food chemistry》2004,52(15):4853-4859

The bioactivity of Israeli Jaffa blond (Shamouti) fresh orange and Israeli Jaffa red Star Ruby (Sunrise) grapefruit juices was investigated in vitro and in vivo. The contents of bioactive compounds of these juices were determined. The influence of bioactive compounds on plasma lipids and plasma antioxidant activity in rats fed cholesterol-containing and cholesterol-free diets was assessed. Significant differences in the contents of dietary fibers were not found. The contents of total polyphenols, flavonoids, and anthocyanins in fresh orange and grapefruit juices were 962.1 +/- 27.2 and 906.9 +/- 27.1; 50.1 +/- 3.3 and 44.8 +/- 3.2; and 69.9 +/- 5.6 and 68.7 +/- 5.5 microg/mL, respectively. The antioxidant potential measured by the scavenging activity against nitric oxide, the beta-carotene-linoleate model system (beta-carotene), and the 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) diamonium salt assays was higher in orange juice but not significantly. A high level of correlation between contents of total polyphenols and flavonoids and antioxidant potential values of both juices was found. Diets supplemented with orange and to a lesser degree with grapefruit juices improved plasma lipid metabolism only in rats fed added cholesterol. However, an increase in the plasma antioxidant activity was observed in both groups. In conclusion, fresh orange and grapefruit juices contain high quantities of bioactive compounds, which guarantee their high antioxidant potential, and the positive influence on plasma lipid metabolism and plasma antioxidant activity could make fresh orange and grapefruit juices a valuable supplement for disease-preventing diets.  相似文献   

Severe psoroptic mange and endoparasitism in a Nubian doe.          下载免费PDF全文

J Lofstedt  S D White  D S Garlick    P Hanna 《The Canadian veterinary journal. La revue veterinaire canadienne》1994,35(11):716-718

  相似文献   

Immunisation of pregnant sows with a live Salmonella cholerae suis vaccine     

J. Hanna  W.A. Ellis  J.J. O&#x;Brien 《Veterinary microbiology》1979,3(4):303-309

Sows were immunised subcutaneously with a live Salmonella cholerae suis (SCS) vaccine prior to farrowing. Serological tests demonstrated a high level of SCS colostral antibodies at parturition. Piglet sera negative for antibodies at birth contained a high level of SCS antibodies 24 h after the ingestion of vaccinated sow colostrum. Experiments were carried out to establish if these maternally derived antibodies could protect the piglets against an intranasal (IN) challenge with a field isolate of SCS. It was concluded that pregnant sows could be safely immunised with a live SCS vaccine thus providing their piglets with a passive immunity which protected them against an intranasal challenge.  相似文献   

Prince Edward Island. Papillomatous digital dermatitis in a Canadian dairy herd.   总被引：1，自引：1，他引：0       下载免费PDF全文

P Hanna  J Lofstedt    P Duivenvoorden 《The Canadian veterinary journal. La revue veterinaire canadienne》1994,35(10):657

  相似文献   

Use of PPD and phosphatide antigens in an ELISA to detect the serological response in experimental bovine tuberculosis   总被引：3，自引：0，他引：3  

J Hanna  S D Neill  J J O'Brien 《Research in veterinary science》1989,47(1):43-47

Five calves from tuberculosis free herds were each inoculated intranasally with 10(6) viable organisms of a field isolate of Mycobacterium bovis. Four of the calves developed acute tuberculosis. ELISAs employing protein and phosphatide extracts of M bovis as antigens were used to monitor the humoral response of the infected calves. Fourteen days after infection there was a dramatic increase in the level of antibodies demonstrated by the phosphatide antigen. This increase coincided with the first appearance of signs of the disease. The results suggest that the phosphatide antigen may be of potential value in detecting a humoral response, if present, in cattle infected with M bovis. The tests employing the protein antigen demonstrated a humoral response in only one of the infected calves and emphasises the importance of antigen selection to detect antibodies in tuberculous animals.  相似文献   

Use of immunosorbent-purified antigens of Fasciola hepatica in enzyme immunoassays   总被引：1，自引：0，他引：1  

A Trudgett  A Anderson  R E Hanna 《Research in veterinary science》1988,44(2):262-263

A monoclonal antibody specific for the T1 tegumental antigen of Fasciola hepatica was used as a solid-phase immunosorbent for the purification of T1 antigen from homogenised mature F hepatica. Material fractionated by this technique was successfully used in enzyme-linked immunoassays to detect antibodies to F hepatica in sera from sheep and cattle. Species differences in response to infection by F hepatica were demonstrated.  相似文献   

Germination of <Emphasis Type="Italic">Plasmodiophora brassicae</Emphasis> resting spores stimulated by a non-host plant     

Hanna?FribergEmail author  Jan?Lagerl?f  Birgitta?R?mert 《European journal of plant pathology / European Foundation for Plant Pathology》2005,113(3):275-281

Plant-induced germination of Plasmodiophora brassicae resting spores was studied in a laboratory experiment. Spore reaction was analysed in nutrient solution with exudates from growing roots of different plant species – one host plant (Brassica rapa var. pekinensis) and four non-host plants (Lolium perenne, Allium porrum, Secale cereale and Trifolium pratense) – and in controls with distilled water and nutrient solution. It was found that root exudates from L. perenne stimulated spore germination more than exudates from the other plants, including those from the host plant. The effect could not be explained by differences in the nutritional composition of the solutions due to differential uptake of the plant species, or by differences in root activity, measured as exudation of soluble sugars. This is the first time such a separation of factors has been done in analysing the influence of plants on P. brassicae germination. Although stimulation of P. brassicae resting spore germination is not restricted to the presence of host plants, it seems to vary depending on the plant species.  相似文献