猪流行性腹泻的诊断与综合防治     

黄捷  王令 《广东畜牧兽医科技》2003,28(4):34-36

猪流行性腹泻(Porcine epidemic diarrhea,PED)是由猪流行性腹泻病毒(PEDV)引起的一种以腹泻、呕吐和脱水为主要特征、与传染性胃肠炎(Transmissible gastroenteritis,TGE)极相似的急性肠道传染病,此病最早在英国报道,随后,欧洲许多国家如比利时、捷克、匈牙利、西德等地均被发现,被称流行性病毒腹泻(Epidemic virus diarrhea,EVD),直到1978年Pensaert等才从病料中分离出这种病毒来。我国从80年代以来,广东、上海、北京、福建、青海、辽宁等地都多次报道有PED的流  相似文献   

Influence of environment on adrenal cortical response to ACTH stimulation in clinically normal dogs.     

G C Vial  G H Stabenfeldt  C E Franti  G V Ling 《American journal of veterinary research》1979,40(7):919-921

Effect of testing environment on adrenal cortical responses to an injection of ACTH in clinically normal dogs was examined in three locations, presumably of increasing order of stress elicitation: in a home; veterinary hospital (VH), 4 hours in a cage; and VH, overnight in a cage. Basal cortisol (hydrocortisone) values for plasma were significantly lower (P less than 0.001) for the home group (1.8 microgram/dl) when compared with values for the VH, 4-hour cage (3.8 microgram/dl) or the VH, overnight cage (3.9 microgram/dl) groups. However, significant differences (P greater than 0.05) were not observed 2 hours after ACTH admininstration for the home group (13.7 microgram/dl); VH, 4-hour cage group (14.8 microgram/dl); or VH, overnight cage group (16.0 microgram/dl). Responses of individual dogs were consistent (P less than 0.005). The testing environment did not markedly affect results of adrenal cortical function tests for dogs when ACTH stimulation was utilized. The response of dogs to ACTH, as monitored by immunologic assay techniques (competitive protein-binding assay or radioimmunoassay), was consistent and was useful as a diagnostic aid for adrenal malfunction.  相似文献   

浅谈HACCP在机制针形名茶中的应用     

邓敏  徐泽  张玲  胡翔 《西南园艺》2005,33(4):41-42

对HACCP作简要介绍，根据HACCP体系的要求，分析了机制针形名茶过程中各个环节可能出现的危害因素，用HACCP的原理确立了关键控制点，并且建立了相应的控制措施。  相似文献   

几种植物提取物和药剂对桃蚜和萝卜蚜生长发育的影响   总被引：9，自引：1，他引：9  

周琼  梁广文  曾玲 《中国蔬菜》2005,1(2):15-17

研究了苍耳提取物和合果芋(白蝴蝶)提取物以及常用天然药剂机油乳剂和 0. 3 %印楝素乳油对桃蚜和萝卜蚜实验种群的影响,两种提取物和药剂对桃蚜一龄若蚜的影响依次为: 0 3 % 印楝素乳油 (2 000×) >苍耳提取物(DW 0 04g·mL-1 ) >机油乳剂(400 ×) >白蝴蝶提取物(DW 0 04g·mL-1 ),对萝卜蚜一龄若蚜的影响依次为: 0. 3 %印楝素乳油(2 000×) >机油乳剂 ( 400×) >苍耳提取物 (DW 0. 04g·mL-1 ) >白蝴蝶提取物(DW 0. 04g·mL-1 );苍耳提取物对萝卜蚜实验种群有明显干扰控制作用,处理后萝卜蚜下代的种群增长量仅为对照的 24. 9 %;此外,苍耳提取物可延缓桃蚜和萝卜蚜的生长发育进程,使发育历期延长,对桃蚜的作用效果比对萝卜蚜的作用效果强;白蝴蝶提取物对萝卜蚜的实验种群也有一定的干扰作用,并可延长桃蚜的发育历期。  相似文献   

松口蘑的研究进展   总被引：2，自引：0，他引：2  

邹莉  李玲  池玉杰 《中国食用菌》2005,24(3):11-13

详细论述了松口蘑的生物学特性、栽培技术及液体深层发酵技术，并探讨了过氧化物酶和酯酶同工酶技术、原生质体技术、基因工程技术与DNA指纹技术在松口蘑研究中的应用及发展趋势。  相似文献   

安徽省鸡免疫抑制性疾病的流行病学调查   总被引：8，自引：0，他引：8  

杨克礼韦平  潘玲蒋玲艳  李莉萍阳秀英磨美兰  韦天超 《中国兽医科技》2005,35(8):665-670

采集了安徽省6个主要养鸡地区65群鸡的185只病鸡共986份组织样品，对可引起免疫抑制性疾病的5种最常见病毒进行了PCR检测。结果，传染性腔上囊病病毒（IBDV）、鸡传染性贫血病毒（CIAV）、马立克氏病病毒（MDV）、禽白血病病毒（ALV）和网状内皮增生症病毒（REV）的群阳性率和个体阳性率分别为73．08％和40．91％、46．15％和25．95％、41．54％和17．84％、18．46％和7．57％、13．85％和4．86％，其中被检鸡之二重或多重混合感染的总阳性率为24．33％。调查结果证实，免疫抑制性疾病在安徽省的商业鸡群中普遍存在，并与鸡群疾病多而复杂、损失大相关。  相似文献   

Effects of phosphodiesterase inhibitor-induced insulin resistance on glucose and lipid metabolism in rats     

YANG Gang-yi  LI Ling  Peter Chung  Gangther Boden 《园艺学报》2003,19(7):875-880

AIM:To investigate the effects of milrinone (a selective phosphdiesterase III inhibitor PDE₃) on insulin secretion, blood glucose, plasma free fatty acids (FFA) and dose-response relationship, and assess possible effects of milrinone on glucose metabolism and insulin sensitivity in conscious rats.METHODS:The catheterized nonstressed rats were administered by the varying doses of milrinone (1, 5, 25 μmol/kg) and were compared with controls. A hyperinsulinaemic- euglycaemic clamp was established in awake rats, and milrinone(25 μmol/kg) and 25% dimethyl sulfoxide (DMSO, as a control) were given at 120 min during hyperinsulinaemic- euglycaemic clamp. Glucose turnover was decided by gas chromatograph mass spectrometer (GC-MS).RESULTS:After dosing, plasma FFA levels in 3 milrinone groups significantly increased compared with the controls and before dosing. The percentages of elevation of FFA by the different milrinone doses were very similar, 50%, 52%, 55% for 1, 5, 25 μmol/kg respectively at 2 min after dosing. Plasma insulin levels were significantly elevated in the 5 and 25 μmol/kg groups, and the effect of milrinone on glucose concentration was detectable only 25 μmol/kg group. During hyperinsulinaemic clamp, there were significant increase in plasma FFA (from 173.1±15.2 to 633.8±87.3 μEq/L) and hepatic glucose production (HGP), and a significant decrease in glucose infusion rates (GIR) (to about 21%).CONCLUSION:These data suggest that milrinone impaires the abilities of insulin to suppress lipolysis and HGP, and insulin-mediated glucose utilization in peripheral tissue. Therefore, milrinone administration may induce an acute insulin resistancein vivo.  相似文献   

乡村生态学研究的尺度与等级特征   总被引：2，自引：0，他引：2  

何念鹏  周道玮  吴泠 《干旱区资源与环境》2002,16(2):22-26

乡村生态学 ( Village ecology)的尺度和等级特征依赖一定空间区域 ,不同尺度所研究村落的特征也不尽相同 ,空间尺度的大小很大程度上决定于所研究村落的复杂性、稳定性及特征。在特定的空间尺度下 ,乡村的组成要素随着时间尺度的变化而变化 ,但变化速率受空间尺度大小的制约。乡村生态学研究具有等级结构 ,空间尺度决定所研究单元的等级位置。行政村 (中心村 )和自然村 (自然屯 )是乡村生态学研究的基本尺度  相似文献   

营养和环境条件对杏鲍菇菌丝生长的影响   总被引：9，自引：2，他引：9  

宫志远  于淑芳  曲玲 《食用菌学报》2002,9(3):13-17

研究了杏鲍菇菌丝生长的营养需求和环境条件，结果表明，适宜杏鲍菇菌丝生长的最佳碳源是葡萄糖、最佳氮源是黄豆粉，最佳碳氮比是60：1，最佳无机盐是硫酸钙。杏鲍菇菌丝生长的适宜温度为25℃，培养料最适含量为75%，最适pH为5.4。  相似文献   

苹果梨中多酚氧化酶酶学特性的研究   总被引：26，自引：2，他引：26  

程建军  马莺  杨咏丽  凌海波  焦明晶  庄严 《园艺学报》2002,29(3):261-262

 以苹果梨中的多酚氧化酶为研究对象, 对其酶的活性变化及其特性的研究表明, 苹果梨中的多酚氧化酶的最适pH 值是4. 6 , 最适温度是40 ℃; 亚硫酸氢钠、维生素C、柠檬酸对苹果梨中多酚氧化酶的抑制作用依次减弱, 硫酸钠几乎无抑制作用。不同底物和同底物不同浓度影响多酚氧化酶特性。  相似文献