Anti-Neospora caninum antibody detection and vertical transmission rate in pregnant zebu beef cows (Bos indicus): Neospora caninum in pregnant beef cows (Bos indicus)     

Eudes Esteves do Nascimento  Ana Sue SammiJoeleni Rosa Dos Santos  Beatriz de Souza Lima NinoAlexey Leon Gomel Bogado  Alessandra TarodaOdilon Vidotto  João Luis Garcia 《Comparative immunology, microbiology and infectious diseases》2014

The aim of the present study was to evaluate anti-Neospora caninum antibodies and the vertical transmission rate in naturally infected pregnant zebu beef cows (Bos indicus) reared on pasture. The present study began with 200 cows from four farms (50 cows from each farm), and these animals were submitted to timed artificial insemination (TAI). After ultrasonography, 76 pregnant cows were selected, 22, 15, 22, and 17, respectively, from farms 1, 2, 3, and 4. Blood samples were taken from cows thrice during the first, second, and third trimester of gestation, and a blood sample was collected from 31 calves before colostrum milking. From 76 cows 23 (30.3%) had anti-N. caninum antibodies detected by indirect ELISA (Idexx), and 53 (69.7%) did not. Sixty-four cows that initiated the experiment were negative to N. caninum and 11 became positive either during the second or third trimester of gestation, this mean an infection incidence of 17.2% (11/64). OD for ELISA was higher (OD = 2.08) during the second and third (OD = 2.10) trimesters of pregnancy when compared with the first (OD = 1.81), however, there were no statistical differences (P = 0.45). The vertical transmission was calculated to be 29.0% (9/31), and the risk of vertical transmission of N. caninum in seropositive dams was 26.25 times higher than seronegative animals (OR = 26.25, 2.38 < OR < 289, P = 0.007). In conclusion, the rate of vertical transmission of N. caninum in pregnant zebu beef cows was 29%, and the risk was 26.25 higher in seropositive dams relative to than seronegative animals.  相似文献   

Effects of dexmedetomidine on pulse pressure variation changes induced by hemorrhage followed by volume replacement in isoflurane‐anesthetized dogs     

Miriely S. Diniz Med Vet  Francisco J. Teixeira‐Neto Med Vet  MSc  PhD  Thaísa D. Cândido Med Vet  Felipe S. Zanuzzo Med Vet  Lívia R. Teixeira Med Vet  MSc  Adriana V. Klein Med Vet  Paulo do Nascimento Jr MSc  PhD 《Journal of Veterinary Emergency and Critical Care》2014,24(6):681-692

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Qualitative risk assessment of transmission pathways of highly pathogenic avian influenza (HPAI) virus at live poultry markets in Dhaka city,Bangladesh     

SK Shaheenur Islam  Holy Akwar  Md. Mehedi Hossain  Md. Abu Sufian  Md. Zakiul Hasan  Shovon Chakma  Tongkorn Meeyam  Warangkhana Chaisowwong  Veerasak Punyapornwithaya  Nitish C. Debnath  Eric Brum  Duangporn Pichpol 《Zoonoses and public health》2020,67(6):658-672

Analysis of environmental samples obtained from the Live Poultry Markets (LPMs) of Dhaka City, Bangladesh, has revealed that the highest degree of prevalence of highly pathogenic avian influenza A (HPAI, H5N1), besides other subtypes of the LPAI virus, poses the plausible risk of transmission of these viruses between human and poultry species. The present study was conducted using the OIE risk analysis framework to assess the risk level of each pathway successively. The estimated risk parameters were integrated towards to obtain the overall risk level for each specific HPAI transmission pathway using the matrix adapted by Cristobel Zepeda accompanying other expert consultations. The relevant data obtained from published and unpublished sources, together with survey data of field observations, were used to formulate and confirm the risk pathways and their associated risks. The results revealed that the risk of the release of the HPAI virus was medium when exposure was high. Additionally, the consequence would be considered very high with a medium degree of uncertainty for all parameters. Ultimately, the overall risk for transmission was estimated as medium with a medium degree of uncertainty. The findings of this study reveal that there is a significant threat that HPAI virus transmission could occur among poultry and humans and effectively sustain within the environment of the LPMs. Our findings are primarily focused on public health considerations, the hygienic slaughter of poultry and the relevant cleaning and sanitation practices conducted in the LPMs to support evidence‐based decision‐making processes. The findings of the study have the potential to be used to formulate effective risk reduction measures and can be further adapted in low‐resource settings without major infrastructural changes required of the LPMs. All of which would reduce the risk of HPAI virus release and further lessen the degree of exposure and transmission in established LPMs.  相似文献   

Influence of cultivar on quality parameters and chemical composition of strawberry fruits grown in Brazil   总被引：1，自引：0，他引：1  

Cordenunsi BR  Oliveira do Nascimento JR  Genovese MI  Lajolo FM 《Journal of agricultural and food chemistry》2002,50(9):2581-2586

Six strawberry cultivars grown on the same commercial plantation in Brazil were evaluated for their chemical composition and quality attributes at the ripe stage. The profiles of the main soluble sugars, ascorbic acid, and anthocyanins were also obtained during the developmental stages. Results showed significant differences among cultivars in all of the investigated parameters. Cv. Campineiro showed an average value for texture of 0.63 N, half the value found for cv. Oso Grande. Anthocyanin content ranged from 13 (cv. Campineiro) to 55 (cv. Mazi) mg/100 g. Total ascorbic acid found for cv. Campineiro (85 mg/100 g) was twice the amount found in cv. Dover (40 mg/100 g). Fructose was the predominant soluble sugar in almost all cultivars. The proportion among the main soluble sugars (fructose, sucrose, and glucose) was similar for Oso Grande and Toyonoka cultivars. The flavonol content (quercetin plus kaempferol derivatives) ranged from 2.7 to 7.1 mg/100 g, with a mean value of 6.1 mg/100 g, whereas free ellagic acid ranged from 0.9 to 1.9 and total phenolics varied from 159 to 289 (mean 221) mg/100 g.  相似文献   

Intake,digestibility, ingestive behavior,and nitrogen balance of goats fed with diets containing residue from tamarind fruit     

Galvão  J. M.  Silva  T. M.  Silva  W. P.  Pimentel  P. R. S.  Barbosa  A. M.  Nascimento  T. V. C  Lima  A. G. V. O.  Bezerra  L. R.  Oliveira  R. L. 《Tropical animal health and production》2020,52(1):257-264

This study is aimed at determining the maximum inclusion level of tamarind (Tamarindus indica) residues in the diet of goats on intake, digestibility, ingestive behavior, and nitrogen (N) balance. Twenty-four crossbred (Boer × undefined breed) castrated goat kids (5 months old and with an initial weight of 23.9?±?0.3 kg) were assigned in a completely randomized design (4 treatments and 6 replicates). Diets consisted of Tifton 85 (Cynodon sp.) hay as the roughage (400 g/kg) source and concentrate (600 g/kg); the levels of tamarind residue inclusion were 0.0, 7.0, 14.0, and 21.0% on a dry matter (DM) basis. The experimental period lasted 23 days (15 of adaptation and 8 of sampling). Inclusion of tamarind residue in the goat kid diets did not affect (P?>?0.05) the intake and digestibility of DM, crude protein, neutral detergent fiber (NDF), and total digestible nutrient; intake of N, urinary N, and retained N (g/day); time spent ruminating; numbers of times/day feeding, ruminating, or idling; eating efficiency of DM and NDF; number of boluses/day; and amount (g) of DM/bolus. However, there were a linear reduction in ether extract digestibility (P?=?0.011) and a linear decreasing trend in non-fibrous carbohydrate digestibility (P?=?0.083). The addition of tamarind residue had a positive linear effect (P?=?0.041) on the time spent feeding and promoted a decreasing linear trend for the time spent idling (P?=?0.063). It is recommended to include the residue from tamarind fruit at a level of 21% in diets for goat kids, as it does not affect nutrient intake and digestibility and the N balance.

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Live and frozen Artemia nauplii for catfish Lophiosilurus alexandri (Steindachner, 1876) larvae in different salinities     

Nascimento  Maria Da Paixão  Schorer  Marianne  dos Santos  José C. E.  Rocha  Maíra S. A.  Pedreira  Marcelo Mattos 《Tropical animal health and production》2020,52(2):653-659

Tropical Animal Health and Production - The objective of the study was to verify the combination of live and frozen Artemia nauplii, in different salinized water, and its influence in growth and...  相似文献   

Coelioscopic-assisted prefemoral ovariosalpingectomy in a d`Orbigny slider (Trachemys dorbigni) using a digital otoscope     

Bernardo Nascimento ANTUNES  Diego DA COSTA  Michelli Westphal DE ATAIDE  Allana Valau MOREIRA  Luis Fernando PEDROTTI  Francisco Schulz JÚNIOR  Cassiano Schmitz NHOATO  Renan Alves STADLER  Marco Augusto Machado SILVA  Maurício Veloso BRUN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(12):1802

The purpose of this study was to assess the unilateral prefemoral coelioscopic-assisted approach for ovariosalpingectomy in a d`Orbigny slider (Trachemys dorbigni) using a digital otoscope. Twenty healthy turtles were randomly assigned to one of two groups, for right (GR; n=10) or left (GL; n=10) prefemoral access, for coelioscopic-assisted ovariosalpingectomy. Anesthesia and surgery times, body weight, and ovary/oviduct weight data were recorded. Anesthesia and surgery times did not differ significantly between the groups. Wound closure was the most time-consuming surgical step. Ovary and body weights significantly affected the exposure time of the ipsilateral and contralateral ovaries, respectively. Two intraoperative complications were reported. All the animals recovered uneventfully. The digital otoscope can be safely and effectively used for coelioscopic-assisted single-access, unilateral prefemoral ovariosalpingectomy in d`Orbigny slider.  相似文献   

Analytical characterization of madeira wine     

Nogueira JM  Nascimento AM 《Journal of agricultural and food chemistry》1999,47(2):566-575

For a correct characterization of Madeira wine commercially available in the market, 52 samples having different types and ages, representative of the delimited region of Madeira (liquor wine of quality produced in delimited region), were analyzed in relation to physicochemical and sensorial parameters. Standard methodology for spirits and alcoholic beverages as well as a council of tasters were adopted respectively to quantify such parameters, according to European Union regulations. The main physicochemical parameters analyzed demonstrated that Madeira wine represent a high quality beverage without toxicological risks, as all constituents have in general contents clearly below the maximum concentration admissible by the national and/or international rules. A reasonable differentiation of properties could be achieved between samples having different types and ages, when principal component, discriminant and cluster analyses were applied to the analytical data, especially for physicochemical parameters.  相似文献   

A new species of Trichuris from Thrichomys apereoides (Rodentia: Echimyidae) in Brazil: Morphological and histological studies     

Torres EJ  Nascimento AP  Menezes AO  Garcia J  dos Santos MA  Maldonado A  Miranda K  Lanfredi RM  de Souza W 《Veterinary parasitology》2011,176(2-3):226-235

Trichuris thrichomysi n. sp., recovered from the cecum of the wild rodent Thrichomys apereoides from a transition zone between the Atlantic Forest and Cerrado morfoclimatic domains, and its life cycle observed under experimental conditions are described. This new species is closely related to Trichuris travassosi, Trichuris chiliensis and Trichuris fulvi, but can be distinguished from them mainly by differences in the posterior end of males. Details of the surface such as the bacillary gland, cuticular inflations and several morphological details obtained by scanning electron microscopy and field emission scanning electron microscopy confirmed the characteristics that differentiate the new species. The histopathology of the intestinal wall of naturally infected rodents is also reported. The present study extends the geographical distribution of T. thrichomysi n. sp to the Pantanal ecosystem and reports a new host, Thrichomys pachiurus.  相似文献   

In silico structural characteristics and α-amylase inhibitory properties of Ric c 1 and Ric c 3, allergenic 2S albumins from Ricinus communis seeds     

Do Nascimento VV  Castro HC  Abreu PA  Oliveira AE  Fernandez JH  Araújo Jda S  Machado OL 《Journal of agricultural and food chemistry》2011,59(9):4814-4821

The major Ricinus communis allergens are the 2S albumins, Ric c 1 and Ric c 3. These proteins contain a trypsin/α-amylase inhibitor family domain, suggesting that they have a role in insect resistance. In this study, we verified that Ric c 1 and Ric c 3 inhibited the α-amylase activity of Callosobruchus maculatus, Zabrotes subfasciatus, and Tenebrio molitor (TMA) larvae as well as mammalian α-amylase. The toxicity of 2S albumin was determined through its incorporation in C. maculatus larvae as part of an artificial diet. Bioassays revealed that 2S albumin reduced larval growth by 20%. We also analyzed the tridimensional structures of Ric c 1 and Ric c 3 by (a) constructing a comparative model of Ric c 1 based on Ric c 3 NMR structure and (b) constructing the theoretical structure of the Ric c 1-TMA and Ric c 3-TMA complexes. Our biological and theoretical results revealed that Ric c 1 and Ric c 3 are a new class of α-amylase inhibitors. They could potentially be used to help design inhibitors that would be useful in diverse fields, ranging from diabetes treatment to crop protection.  相似文献