Capturing genetic variability and selection of traits for heat tolerance in a chickpea recombinant inbred line (RIL) population under field conditions     

Pronob J. Paul  Srinivasan Samineni  Sobhan B. Sajja  Abhishek Rathore  Roma R. Das  Sushil K. Chaturvedi  G. R. Lavanya  Rajeev K. Varshney  Pooran M. Gaur 《Euphytica》2018,214(2):27

Chickpea is the most important pulse crop globally after dry beans. Climate change and increased cropping intensity are forcing chickpea cultivation to relatively higher temperature environments. To assess the genetic variability and identify heat responsive traits, a set of 296 F_8–9 recombinant inbred lines (RILs) of the cross ICC 4567 (heat sensitive) × ICC 15614 (heat tolerant) was evaluated under field conditions at ICRISAT, Patancheru, India. The experiment was conducted in an alpha lattice design with three replications during the summer seasons of 2013 and 2014 (heat stress environments, average temperature 35 °C and above), and post-rainy season of 2013 (non-stress environment, max. temperature below 30 °C). A two-fold variation for number of filled pods (FPod), total number of seeds (TS), harvest index (HI), percent pod setting (%PodSet) and grain yield (GY) was observed in the RILs under stress environments compared to non-stress environment. A yield penalty ranging from 22.26% (summer 2013) to 33.30% (summer 2014) was recorded in stress environments. Seed mass measured as 100-seed weight (HSW) was the least affected (6 and 7% reduction) trait, while %PodSet was the most affected (45.86 and 44.31% reduction) trait by high temperatures. Mixed model analysis of variance revealed a high genotypic coefficient of variation (GCV) (23.29–30.22%), phenotypic coefficient of variation (PCV) (25.69–32.44%) along with high heritability (80.89–86.89%) for FPod, TS, %PodSet and GY across the heat stress environments. Correlation studies (r = 0.61–0.97) and principal component analysis (PCA) revealed a strong positive association among the traits GY, FPod, VS and %PodSet under stress environments. Path analysis results showed that TS was the major direct and FPod was the major indirect contributors to GY under heat stress environments. Therefore, the traits that are good indicators of high grain yield under heat stress can be used in indirect selection for developing heat tolerant chickpea cultivars. Moreover, the presence of large genetic variation for heat tolerance in the population may provide an opportunity to use the RILs in future-heat tolerance breeding programme in chickpea.  相似文献   

Effect of dietary Curcuma longa on enzymatic and immunological profiles of rohu,Labeo rohita (Ham.), infected with Aeromonas hydrophila     

Swagatika Sahu  Basanta Kumar Das  Bibhudendu Kumar Mishra  Jyotirmayee Pradhan  Surya Kanta Samal  Niranjan Sarangi 《Aquaculture Research》2008,39(16):1720-1730

The aim of this study was to evaluate the dietary dosages of turmeric that enhance immune response and disease resistance against the opportunistic pathogens Aeromonas hydrophila in Labeo rohita fingerlings. Hence, four different dosages of turmeric at 0.1, 0.5, 1.0 and 5.0 g kg^?1 feed were given to the fingerlings of L. rohita for 60 days at 4% body weight. At every 20‐day interval, different biochemical, haematological, enzymatic and immunological parameters of fish were evaluated. After 60 days, fish were challenged with A. hydrophila. The mortality (%) was recorded on the tenth day post challenge. Most of the immune parameters including lysozyme activity, superoxide anion production and serum bactericidal activity were significantly (P<0.05) higher on 60 days of feeding of 1.0 g of turmeric per kg of feed. Challenge study indicated 100% and 89% survivability in the group of fish fed with 5.0 and 1.0 g of turmeric per kg of feed respectively. Feeding of turmeric might have maintained long‐term protection in fish by elevating the nonspecific immune system such as Nitroblue tetrazolium (NBT), lysozyme and serum bactericidal activity. The result showed that turmeric at a dose of 1.0 g kg^?1 feed for 60 days provided the greatest protection to pathogen challenge.  相似文献   

Genetic variations in survival of rohu carp (Labeo rohita, Hamilton) after Aeromonas hydrophila infection in challenge tests     

K. Das Mahapatra  B. Gjerde  P.K. Sahoo  J.N. Saha  A. Barat  M. Sahoo  B.R. Mohanty  J. degrd  M. Rye  R. Salte 《Aquaculture (Amsterdam, Netherlands)》2008,279(1-4):29-34

A challenge test against Aeromonas hydrophila was conducted using 2062 rohu carp (Labeo rohita) fingerlings obtained from 52 dams and 87 sires (87 full-sib families) of two year-classes (2003 and 2004). Attempts to establish a cohabitant challenge model were not successful. Therefore, fish were challenged by intraperitoneal injection with A. hydrophila in two replicate tanks per year-class, and dead fish were collected hourly. The mortality reached its peak at 16–22 h after challenge and had almost completely stopped after 58 h. The test was terminated after 382 h at which the average survival was 43.9 and 48.9% in the two 2003 year-class tanks, and 77.1 and 35.8% in the two 2004 year-class tanks. Heritability estimates for survival were obtained from sire and dam threshold models (THR) and sire and dam linear repeatability models (LINR). For both types of models the heritability estimates based on data from single tanks were not consistent. For both year-classes, data from one of the two challenged tanks demonstrated significant additive genetic variation in survival during the A. hydrophila infection, whereas the heritability estimates were not significantly different from zero for the other tank. Further, genetic correlation between survivals in the two replicate tanks in each year-class was not significantly different from zero. The differential results from the replicate tanks demonstrate that additional challenge test experiments are needed before firm conclusions can be drawn about the magnitude of additive genetic variation for survival to aeromonasis in rohu carp. A cohabitant challenge model that allows the testing of important defence mechanisms in the skin and mucous membranes of the fish might have been more appropriate. To establish a valid cohabitant challenge model for rohu carp should be given high priority.  相似文献   

Extension of shelf life of tuberose flowers using a combination of gamma irradiation and generally regarded as safe (GRAS) preservatives and assessment of antimicrobial potency of senesced flowers     

Probir Kumar Ghosh  Satadal Das 《The Journal of Horticultural Science and Biotechnology》2017,92(2):130-145

Tuberose cut flowers, available as loose flowers, were treated with gamma (γ) irradiation and generally regarded as safe (GRAS) preservative solutions for extension of shelf life. The flowers were packaged in low-density polyethylene bags, heat sealed and stored at 23 ± 2°C, 80% relative humidity (RH) and 4 ± 1°C, 40% RH, respectively. The flowers stored at these two temperature regimes were subjected to sensory evaluation and biochemical analyses. From these assessments, the longest shelf life of tuberose flowers was found to be 8 days at 23 ± 2°C, 80% RH (compared to 4 days for control) and 24 days at 4 ± 1°C, 40% RH (compared to 8 days for control) using combination treatment of low dose γ-irradiation (0.02 kGy) and preservative solutions (4% sucrose and 0.02% CaCl₂). Ethanolic extract of tuberose flowers of the most shelf stable set (stored at 4 ± 1°C), i.e. at the end of 24 days, showed antimicrobial potency against the common skin pathogen Staphylococcus aureus (ATCC 25923 and MDR strains), suggesting utility of the senesced tuberose flowers for therapeutic applications. This preservation technique would promote export of tuberose flowers by extension of their shelf lives and allow utilization of these flowers, post senescence.  相似文献   

Segmentation of 3D images of plant tissues at multiple scales using the level set method     

Annamária Kiss  Typhaine Moreau  Vincent Mirabet  Cerasela Iliana Calugaru  Arezki Boudaoud  Pradeep Das 《Plant methods》2017,13(1):114

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Fatty Acid and Non-Fatty Acid Components of the Seed Oil of Celastrus paniculatus willd.     

Virendra S. Rana  Manish Das 《International Journal of Fruit Science》2017,17(4):407-414

The yield and chemical composition of the seed oil of Celastrus paniculatus were determined by solvent extraction and gas chromatography-mass spectrometry. The yield of crude fatty oil was found to be 45.5% in the mature seeds on dry weight basis. Fourteen compounds, constituting 97.57% of the oil, were identified. The oil was rich in unsaturated fatty acids (70.11%) followed by saturated fatty acids (25.2%). Oleic acid (54.42%), palmitic acid (20.0%), linoleic acid (15.51%), and stearic acid (4.18%) were the main fatty acids in the oil together with four other minor fatty acids. The current study identifies the novel compounds not previously reported in C. paniculatus oil, including 1,4-benzenediol (0.46%), cinnamic acid (0.15%), 2,6-di-tert-butyl-p-benzoquinone (0.03%), butylated hydroxytoluene (0.4%), and eudalene (0.16).  相似文献   

Identifying climate refugia for 30 Australian rainforest plant species,from the last glacial maximum to 2070     

Das  Sourav  Baumgartner  John B.  Esperon-Rodriguez  Manuel  Wilson  Peter D.  Yap  Jia-Yee S.  Rossetto  Maurizio  Beaumont  Linda J. 《Landscape Ecology》2019,34(12):2883-2896

Landscape Ecology - Climate refugia—areas that remain suitable for species during periods of climate disruption—have played an important role in species persistence over time....  相似文献   

Hydrus-1D model for simulating water flow through paddy soils under alternate wetting and drying irrigation practice     

Shekhar  Shashank  Mailapalli  Damodhara Rao  Raghuwanshi  Narendra Singh  Das  Bhabani Sankar 《Paddy and Water Environment》2020,18(1):73-85

Paddy and Water Environment - Alternate wetting and drying (AWD) irrigation practice in paddy cultivation requires a safe soil moisture stress level in the root zone for which irrigation water...  相似文献   

Heat shock protein 70 expression in different tissues of Cirrhinus mrigala (Ham.) following heat stress   总被引：2，自引：0，他引：2  

Paromita Das  Akhil Gupta  & Sanjib Kumar Manna 《Aquaculture Research》2005,36(6):525-529

Members of heat shock protein 70 (HSP70) are highly conserved proteins of about 70 kDa and play important roles in protein folding. Levels of these proteins increase when cells are under stress. Environmental temperature influences both the basal and induced levels of HSPs. However, studies on HSPs in fishes from a tropical country such as India are lacking. In the present study, Indian major carp (IMC) Cirrhinus mrigala (Ham.) acclimatized at 25±2°C had high levels of HSP70, viz., 1.2–1.3 ng μg^?1 total protein in kidney and gill and 4.2–5.3 ng μg^?1 total protein in liver and brain tissues, indicating the presence of biochemically significant levels of stress. However, maintenance of acclimatized fish at 17°C for up to 48 h did not lead to a significant decrease in stress protein levels. A heat shock at 37°C for up to 48 h resulted in only two to threefold increase in HSP70 levels in these organs. Although the increase in HSP70 levels was apparent from the first hour of heat stress in all these tissues, the increase was significant from the second hour in the brain, the sixth hour in liver and kidney and the 20th hour in the gills.  相似文献   

Impact of Aeration on the Growth Performance of Silver Barb,Puntius gonionotus,during Fingerling Rearing     

Pratap Chandra Das  Joykrushna Jena  Bibhudatta Mishra  Bikash Kumar Pati 《Journal of the World Aquaculture Society》2012,43(1):128-134

Impact of aeration on growth of silver barb, Puntius gonionotus during fingerling rearing was studied through a 100‐d rearing experiment conducted in 18 concrete tanks of 50 m² (10 × 5 × 1.2 m) size. Fry (0.74 ± 0.27 g, 35 ± 6 mm) were stocked in the experimental tanks at three stocking densities (25, 50, and 75 fry/m²) and were evaluated with and without provision of 6 h (2400–0600 h) of night time aeration. Aeration resulted in higher pH and dissolved oxygen regime and increased fingerling length and weight. The results suggest a rearing density of 75/m² to be ideal for rearing fry to fingerling of this species when aeration is provided, whereas, under non‐aerated condition, rearing the fry to fingerling stage at 50/m² was found advantageous over those at 25 and 75/m².  相似文献