全文获取类型
收费全文 | 324篇 |
免费 | 44篇 |
专业分类
林业 | 20篇 |
农学 | 13篇 |
55篇 | |
综合类 | 18篇 |
农作物 | 15篇 |
水产渔业 | 40篇 |
畜牧兽医 | 170篇 |
园艺 | 2篇 |
植物保护 | 35篇 |
出版年
2023年 | 4篇 |
2022年 | 12篇 |
2021年 | 17篇 |
2020年 | 24篇 |
2019年 | 28篇 |
2018年 | 13篇 |
2017年 | 19篇 |
2016年 | 23篇 |
2015年 | 14篇 |
2014年 | 17篇 |
2013年 | 24篇 |
2012年 | 13篇 |
2011年 | 25篇 |
2010年 | 9篇 |
2009年 | 14篇 |
2008年 | 16篇 |
2007年 | 21篇 |
2006年 | 12篇 |
2005年 | 14篇 |
2004年 | 6篇 |
2003年 | 4篇 |
2002年 | 4篇 |
2001年 | 5篇 |
2000年 | 3篇 |
1999年 | 6篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1976年 | 1篇 |
1973年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有368条查询结果,搜索用时 31 毫秒
31.
Oliveira M Bexiga R Nunes SF Carneiro C Cavaco LM Bernardo F Vilela CL 《Veterinary microbiology》2006,118(1-2):133-140
Biofilm-forming ability has been increasingly recognized as an important virulence factor in Staphylococci, facilitating their persistence in the host, evading its defences and allowing bacterial survival at high antimicrobial concentrations. Staphylococcus aureus remains a major pathogen of chronic mastitis, but in the last years Staphylococcus epidermidis has emerged as a relevant mastitis pathogen. The present work aimed at the evaluation of the biofilm-forming ability of Staphylococci field isolates from bovine subclinical mastitis and at the development of a fluorescent in situ hybridisation (FISH) protocol that would allow the direct observation of biofilm formation in milk samples. The analysis of phenotypic expression in Congo Red Agar (CRA) and by FISH, showed that 37.5% of the S. aureus isolates produced biofilm, while by optical density measurement only 18.75% isolates revealed this phenotype. The results showed a fair agreement according to the kappa coefficient test (kappa = 0.259). Regarding S. epidermidis mastitis isolates, 37.5% revealed the ability to produce biofilm, but only four isolates were positive by all methods. This agreement was moderate (kappa = 0.467). The application of FISH to artificially contaminated milk samples allowed the direct observation of biofilm production by 37.5% isolates, showing total agreement with the CRA results. This method better mimics the in vivo conditions, especially in terms of the presence of calcium and iron, which in high concentrations, respectively, are known to inhibit or induce biofilm production. 相似文献
32.
Evaluation of antiviral activity of phenolic compounds and derivatives against rabies virus 总被引:1,自引:0,他引:1
Chávez JH Leal PC Yunes RA Nunes RJ Barardi CR Pinto AR Simões CM Zanetti CR 《Veterinary microbiology》2006,116(1-3):53-59
Human rabies is a viral disease with a great impact on public health, mainly on account of its fatal course in the majority of cases. Despite the well-established prophylaxis by immunization, rabies is believed to be responsible for 40,000-70,000 human deaths per year, mostly in endemic areas. Palliative support and experimental protocols to avoid death have been employed with no expressive results, with the exception of a recent human case of recovery from rabies. No antiviral drugs are currently available to fight against this infection. In combination with the prophylaxis, an antiviral drug would be useful for human rabies treatment, providing enhanced protection against the encephalitis caused by the virus. Phenolic compounds are derived from the secondary plant metabolism, although they can also be obtained by synthetic processes. Many studies have shown a great range of pharmacological effects for these substances, including vasodilatation, antiallergenic, antiinflammatory and antiviral properties, among others. In this study, the potential in-vitro anti-rabies activity of 24 synthetic phenolic compounds was evaluated using McCoy cells and PV rabies strain. The cytotoxicity (CC50) was assayed by the MTT method and the antiviral activity (IC50) was estimated by the inhibition of viral cytopathic effects. Isoprinosine and ketamine were used as positive controls. The tested compounds showed selectivity indices (SI=CC50/IC50) ranging from 1.0 to 3.9. Six phenolic compounds failed to inhibit the cytopathic effect to any degree, and four showed SI > or = 3.0. According to these results, some probable structure-activity relationships are suggested. It was observed that the presence of free hydroxyl and ether groups influenced the anti-rabies activity. However, additional studies are required to establish these relationships. 相似文献
33.
Biological control of postharvest diseases of fruit 总被引:1,自引:0,他引:1
Carla Alexandra Nunes 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(1):181-196
Postharvest decay in harvested fruit causes considerable economical losses. Fungicides are the primary means to control these
losses. Public concern in food safety and environmental issues and the increase of pathogen resistant populations have enhanced
the interest in developing alternative methods to fungicides to control postharvest fruit decay. During the last two decades
a huge information and advances concerning the selection of antagonists, mode of action, different approaches to enhance biocontrol
activity, formulation and production have been achieved, and some biofungicides are already in the market. It is likely that
several more products will enter the market in the near future, as the result of the biological control research programs
worldwide. Nonetheless, it is necessary to continue finding new potential microorganisms, better understanding the mode of
action, and pathogen, antagonist and host interactions, to increase the potential of biocontrol helping to become a real alternative
to synthetic postharvest fungicides. This article presents an overview of postharvest biological control approaches and explores
new research possibilities to improve biocontrol activity. 相似文献
34.
Jorge Nunes Manuel Madeira Luíz Gazarini José Neves Henrique Vicente 《Agroforestry Systems》2012,84(1):89-100
The changes in the soil nitrate concentration were studied during 2 years in a “montado” ecosystem, in the South of Portugal.
Total rainfall, air and soil temperature and soil water content under and outside Quercus rotundifolia canopy were also evaluated. A cluster analysis was carried out using climatic and microclimatic parameters in order to maximize
the intraclass similarity and minimize the interclass similarity. It was used the k-Means Clustering Method. Several cluster
models were developed using k values ranging between 2 and 5. Thereafter, in each cluster, the data were divided according
to their origin (soil under canopy and open areas, and from surface and deep layers). Multiple regression models were tested
for each cluster, to assess the relationship between soil nitrate concentration and a set of climatic and microclimatic parameters
and the results were compared with models assessed without clustering. The models achieved with data grouped in result of
clustering analysis showed better performance than the models achieved without clustering, mostly for data from open areas
soils. When temperature is low and/or water presents excess or scarcity levels, the data from soils in undercanopy areas,
give rise to models with worst performance than models from open soil areas data. The results obtained for undercanopy area
suggest that nitrification process in soil under Quercus rotundifolia trees influence is more complex than for open areas, and subject to other relevant factors beyond water and temperature. 相似文献
35.
Leilson Rocha Bezerra Severino Gonzaga Neto Ariosvaldo Nunes de Medeiros Tobyas Maia de Albuquerque Mariz Ronaldo Lopes Oliveira Ebson Pereira Cândido Aderbal Marcos de Azevedo Silva 《Tropical animal health and production》2013,45(5):1161-1169
In the present study, the effect of restricting the feed intake for 77 days and subsequent compensatory growth for 50 days of Sindi females were evaluated. Eighteen animals with an initial age of 21 months and a mean weight of 211.7 kg were placed into three groups according to the following alimentary regimens: feed ad libitum, feed restricted to 20 % dry matter, and feed restricted to 40 % dry matter. In the feed-restriction phase, the nutrient intake decreased (P?<?0.001) with an increase in the restriction level. As a consequence, the observed decrease in ingestion and serum concentrations of total protein, albumin and globulin, urea, glucose, calcium, and phosphorus were inversely proportional (P?<?0.001) to the restriction level. Significant differences in the nutrient intake and serum concentration were not observed in the realimentation phase (P?<?0.05). When animals in the control group reached the end of the feed-restriction phase, their weights (P?<?0.05) were similar to those in the 20 % restricted group, and both obtained a final weight that was greater than that of animals in the 40 % restricted group. In the feed-restriction phase, the control group had a similar mean daily weight gain (P?>?0.05) to animals in the 20 % restricted group and (P?<?0.05) 40 % restricted group. However, in the realimentation phase, the 40 % restricted group obtained greater weight gain rates (P?<?0.05), better food conversions, and partial compensatory gains. In particular, none of the restricted groups reached the final weight of the control group. In the feed-restriction phase, ingested nitrogen, nitrogen excreted in urine and feces, nitrogen balance and retained nitrogen decreased (P?<?0.05) with an increase in the restriction level. In the realimentation phase, none of the nitrogen balance variables were influenced by the restriction level (P?<?0.05). Females in the 40 % restricted group presented better food conversion rates and greater weight gains in the realimentation phase. Based on the animals’ compensatory weight gain, a feed-restriction rate of 20 and 40 % can be adopted as a nutritional management practice for prepubescent Sindi females. 相似文献
36.
37.
Alberto J. P. Nunes Hassan Sabry‐Neto Karthik Masagounder 《Journal of the World Aquaculture Society》2019,50(6):1093-1107
The use of supplemental essential amino acids (EAAs) has been shown to provide an opportunity to minimize excess levels of crude protein (CP) in animal feeds. The present study investigated the effect of reducing the amount of CP in low‐fish meal diets (5%) for juvenile Litopenaeus vannamei. Four sets of diets were prepared containing (% on a fed basis, mean ± SD) 31.24 ± 0.71, 33.70 ± 0.41, 36.90 ± 0.44, and 39.63 ± 0.14% CP, with each protein level containing a total dietary methionine (Met) (Met + Cys) level of 0.56 ± 0.02 (1.07 ± 0.23), 0.71 ± 0.01 (1.22 ± 0.20), 0.88 ± 0.02 (1.38 ± 0.22), or 1.04 ± 0.02% (1.55 ± 0.18%). Shrimp of 1.00 ± 0.08 g were stocked in 84 outdoor tanks of 1 m3 at a rate of 100 shrimp/m2 and raised for 75 days. Final survival ranged from 83 to 97% and was unaffected by Met content. Both survival and yield were significantly depressed when shrimp were fed the 31% CP diet. Shrimp grew at a weekly rate of between 0.79 and 0.97 g, achieving a final body weight (BW) in excess of 10.8 g. There was a significant interaction between CP and Met over BW. Shrimp fed 0.56% Met achieved the lowest BW at harvest. Increasing CP beyond 34% did not enhance BW. With a dietary Met content of 0.71%, the highest BW was achieved with 34% CP compared to other levels. There was a significant improvement in Feed Conversion Ratio (FCR) when CP was raised from 31 to 34%. Similarly, dietary Met levels above 0.71% resulted in a significantly better FCR compared to 0.56%. Our study has shown that, if dietary Met (Met + Cys) meets a minimum of 0.71% (1.22%), levels of CP could be reduced from 40 to 34% without adverse effects on shrimp performance. 相似文献
38.
Dubey JP Velmurugan GV Chockalingam A Pena HF de Oliveira LN Leifer CA Gennari SM Bahia Oliveira LM Su C 《Veterinary parasitology》2008,157(3-4):299-305
Until recently, Toxoplasma gondii was considered clonal with very little genetic variability. Recent studies indicate that T. gondii isolates from Brazil are genetically and biologically different from T. gondii isolates from USA and Europe. In the present study, we retyped 151 free range chicken isolates from Brazil including 117 newly isolated samples from 11 geographically areas (Alagoas, Bahia, Ceará, Maranh?o, Paraná, Pernambuco, Rio de Janeiro, Rio Grande do Norte, S?o Paulo, Sergipe, and Rondonia) and 34 previously reported isolates from the very north (Pará) and the very south (Rio Grande do Sul). Ten PCR-RFLP markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico were used to genotype all isolates. Overall analysis of 151 T. gondii isolates revealed 58 genotypes. Half (29/58) of these genotypes had single isolate and the other half of the genotypes were characterized with two or more isolates. Only 1 of 151 isolates was clonal Type I strain and 5 were clonal Type III strains. Two isolates had mixed infections. Clonal Type II strain was absent. One strain was Type II at all loci, except BTUB. The results confirm high genetic diversity of T. gondii isolates from Brazil. 相似文献
39.
Timely onset of postpartum ovarian activity is vital for optimal reproductive performance of dairy cows. Much depends upon genetic constitution of an animal although several factors interplay to govern the onset of postpartum ovarian activity. South Asian zebu cattle have much longer service period when compared with other exotic or crossbred cattle reared in the same Asian environment, which suggests differences in their genetic makeup. However, the cows with same genetic configuration expressed better reproductive potential when reared under different environment, such as in Brazil and Mexico, which suggests the role of extrinsic factors such as management, nutrition, environment and disease conditions. Better management of animals (provision of proper shade, water and housing, efficient oestrous detection and timely insemination), good quality nutrition supplemented with appropriate minerals and vitamins, prevention of diseases (vaccination, deworming, suitable therapeutic interventions) and application of biotechnology have helped in improving postpartum ovarian activity and, therefore, reproductive performance of zebu cattle in Asia. No comprehensive study appears to have been carried out on the various aspects of reproduction in zebu cattle reared under South Asian socio-agro-climatic conditions. This paper is a modest effort to collect what ever information available and to critically review the postpartum ovarian activity in zebu cattle with special reference to the effect of the various managemental practices and pharmacological interventions. 相似文献
40.
Nunes EA Bonatto SJ de Oliveira HH Rivera NL Maiorka A Krabbe EL Tanhoffer RA Fernandes LC 《Research in veterinary science》2008,84(1):62-67
Conjugated linoleic acids (CLA), 9-cis:11-trans and 10-trans:12-cis, have been shown to be able to modify some immune cells parameters and plasma lipids in a variety of experiment models. Since lymphocytes and polymorphonuclear cells (PMNC) have a large spectrum functions in the immune response, the knowledge in this field has to be expanded. Beagle dogs were fed a control diet or a CLA supplemented diet for nine months. Blood was collected for biochemical analysis and lymphocyte and PMNC isolation. PMNC were assayed for lysosome content, phagocytic activity and superoxide anion production. A lymphocyte proliferation capacity assay was done. The CLA fed dogs had a 34% reduction in total cholesterol (P < 0.05), 28% in LDL (P < 0.05) and 28% non-HDL-cholesterol (P < 0.05). Neither of the PMNC parameters evaluated demonstrated significant alteration. Lymphocytes from CLA group increased by 45% their mitotic capacity (P < 0.05). Our study demonstrates that CLA can successfully modify the lipid profile of dogs (monogastrics) when fed at reasonable levels, but did not significantly alter inflammatory function as would generally predicted. Further, we had some indication that CLA modulated T cell responsiveness. 相似文献