Acknowledgments     

Dr. Carl C. Trettin  Dr. W. Michael Aust  Dr. Joe Wisniewski 《Water, air, and soil pollution》1994,77(3-4):xi-xi

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A specific DNA probe which identifies Babesia bovis in whole blood.     

W Petchpoo  P Tan-ariya  V Boonsaeng  C R Brockelman  P Wilairat  S Panyim 《Veterinary parasitology》1992,42(3-4):189-198

A genomic library of Babesia bovis DNA from the Mexican strain M was constructed in plasmid pUN121 and cloned in Escherichia coli. Several recombinants which hybridized strongly to radioactively labeled B. bovis genomic DNA in an in situ screening were selected and further analyzed for those which specifically hybridized to B. bovis DNA. It was found that pMU-B1 had the highest sensitivity, detecting 25 pg of purified B. bovis DNA, and 300 parasites in 10 microliters of whole infected blood, or 0.00025% parasitemia. pMU-B1 contained a 6.0 kb B. bovis DNA insert which did not cross-hybridize to Babesia bigemina, Trypanosoma evansi, Plasmodium falciparum, Anaplasma marginale, Boophilus microplus and cow DNA. In the Southern blot analysis of genomic DNA, pMU-B1 could differentiate between two B. bovis geographic isolates, Mexican strain M and Thai isolate TS4. Thus, the pMU-B1 probe will be useful in the diagnosis of Babesia infection in cattle and ticks, and in the differentiation of B. bovis strains.  相似文献   

冻融中土壤的水热动态     

张转放  赵小雯  华孟 《中国农业大学学报》1992,(3):287-292

根据在北京郊区的试验,认为土壤水冻融过程可分为三阶段,其中第二阶段有聚墒现象,平均聚墒8.6mm,所聚的墒在融化过程中又逐渐消失。气温回升至-2℃左右是冻层下部由冻到融的转折温度。冬灌水保存到来年春季的数量有限,本试验中1m 土层内平均不足20mm。  相似文献   

Detection of TNF alpha receptors on ovine leucocytes by flow cytofluorimetry.     

F P Winstanley 《Research in veterinary science》1992,52(1):129-131

Tumour necrosis factor receptor activity was detected on sheep leucocytes using flow cytofluorimetry and commercially available fluorescent human tumour necrosis factor. The density of receptors per cell and the proportion of cells expressing TNF receptor activity was found to be in the following rank order: monocytes greater than granulocytes greater than lymphocytes.  相似文献   

Pasteurella multocida toxin induces IL-6, but not IL-1 alpha or TNF alpha in fibroblasts.          下载免费PDF全文

S Rosendal  P L Frandsen  J P Nielsen    R Gallily 《Canadian journal of veterinary research》1995,59(2):154-156

Pasteurella multocida toxin (PMT) is the major virulence factor in Progressive Atrophic Rhinitis of swine. Other workers' previous findings that PMT was mitogenic for 3T3 fibroblasts, were confirmed in the present study. In addition, PMT stimulated 3T3 cells to release IL-6, but IL-1 alpha or TNF alpha were not detected in fibroblast supernatants sampled 24, 48, or 72 h after stimulation. In view of the role of IL-6 in osteoclastic bone resorption, these findings provide a new working hypothesis for investigations into the molecular pathogenesis of this important disease.  相似文献   

Correlated Responses in Two Traits when Selection is Practised on their Ratio     

M. Hühn 《Plant Breeding》1992,109(1):28-34

In many fields of application in plant breeding and crop science, ratios of two component traits X and Y are of interest (harvest index in cereals, leaf-to-stem ratio in forage legumes, height-to-diameter ratio in forest trees etc.). When selection is practised on the ratio X/Y of two traits X and Y, the experimenter may be interested in the resulting changes of both trait means. Based on improved approximations for the covariances between X and X/Y and between Y and X/Y and for the variance of X/Y the changes in the means of X and Y can be predicted by applying the regression approach from conventional selection theory. Explicit expressions for these correlated responses in X and Y when selection is practised on their ratio X/Y are derived and discussed. The different outcomes (decrease, zero change or increase) for the selection pressures on X and Y are characterized by phenotypic coefficients of variation of X and Y, phenotypic and genotypic correlations between X and Y and heritabilities of X and Y.  相似文献   

Lupus-Type "Anticoagulant" in a Dog With Hemolysis and Thrombosis   总被引：1，自引：1，他引：0  

Michael S. Stone  Ian B. Johnstone  Marjory Brooks  Trent K. Bollinger  Susan M. Cotter 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(1):57-61

A circulating anticoagulant was detected in a 2-year-old Chesapeake Bay Retriever with hemolytic anemia, nephrotic syndrome, thrombocytopenia, polyarthropathy, and pulmonary thromboembolism. A persistent prolongation of the activated partial thromboplastin time (aPTT) was detected, and it did not correct with repeated administration of fresh frozen plasma. The aPTT was still prolonged, with a 1:1 mixture of patient's plasma and normal dog plasma in vitro, suggesting the presence of a circulating inhibitor. Results of assays to characterize the inhibitor were compatible with those described for the lupus anticoagulant in human patients with systemic lupus erythematosus. Paradoxically, patients having the lupus anticoagulant are at increased risk for thrombosis. Pulmonary thromboembolism has been described as a frequent complication of immune-mediated hemolytic anemia in the dog, and the presence of a circulating anticoagulant should be considered as a potential mechanism.  相似文献   

USE OF MONOCLONAL ANTIBODY GB 24 and PROPIDIUM IODIDE FOR SIMULTANEOUS ASSESSMENT OF ACROSOMAL STATUS and VITALITY IN BOAR SPERMATOZOA     

P. Renard  B. Drénou  J.F. Griveau  D. Le Lannou 《Reproduction in domestic animals》1995,31(1):255-256

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Temporary Bile Diversion in Cats with Experimental Extrahepatic Bile Duct Obstruction     

DARIEN LAWRENCE BSc  BVSC    JAMIE R. BELLAH DVM  Dipiomate ACVS    D. J. MEYER DVM  Dipiomate ACVIM  Dipiomate ACVP  L. ROTH DVM  PhD  Dipiomate ACVP 《Veterinary surgery : VS》1992,21(6):446-451

The use of a cholecystostomy catheter for temporary bile diversion was investigated in four cats with experimentally induced extrahepatic bile duct obstruction. Eighteen days after ligation of the common bile duct, a 6.5 F accordion catheter was placed in the gallbladder with a 22 g Hawkins needle-guide system through a paracostal incision. Biochemical parameters and fasting serum bile acids were monitored for 16 days. There were significant decreases in mean total bilirubin, aspartate aminotransferase, and fasting serum bile acids within 72 hours of bile diversion, and in mean alanine aminotransferase within 96 hours. Attitude and appetite improved, and the catheter was tolerated well. Positive bile cultures developed in three cats. Histologic changes in the gallbladder included mucosal ulcerations, a mixed inflammatory cellular infiltration, and fibrosis of the submucosa.  相似文献   

Two cases of colitis cystica profunda in dogs     

W Kraft  A K Ghermai  D von Bomhard 《Tier?rztliche Praxis》1989,17(3):299-302

Colitis cystica profunda in dogs has been diagnosed in one case only. The two own cases were characterized by repeated, partly bloody diarrhea, vomitus, and painful defecation. The disease was diagnosed by clinical examination and colonoscopy with the ensuing histological examination of biopsy specimens. The disease could be managed by administration of a diet, sulfasalazine and corticosteroids.  相似文献