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31.
The effect of the stage of the ovarian cycle (anoestrus or dioestrus) and of pregnancy on the incidence of gastro‐oesophageal reflux in dogs undergoing ovariohysterectomy 
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下载免费PDF全文 Tilemahos L Anagnostou Ioannis Savvas George M Kazakos Haralabos N Ververidis Dimitra Psalla Charalampos Kostakis Petros Skepastianos Dimitris Raptopoulos 《Veterinary anaesthesia and analgesia》2015,42(5):502-511
ObjectiveTo investigate the potential association of increased blood progesterone (P4) concentrations and/or late pregnancy with the incidence of gastro-oesophageal reflux (GOR), in healthy bitches undergoing ovariohysterectomy under general anaesthesia during anoestrus or dioestrus or during the second half of pregnancy.Study designProspective observational study.AnimalsNinety-four healthy, female, dogs, aged 1–8 years presented for elective ovariohysterectomy.MethodsNon-pregnant animals were classified into group A (anoestrus) (n = 35) if blood P4 concentration was sufficiently low or group D (dioestrus) (n = 26) if blood P4 concentration was sufficiently high. All animals in the second half of pregnancy were classified into group P (n = 33). Acepromazine (0.05 mg kg−1) was administered intramuscularly as preanaesthetic medication, and sodium thiopental (10 mg kg−1, with additional doses if needed) was administered intravenously (IV) for induction of anaesthesia. After endotracheal intubation, halothane (1.1–1.3% end-tidal concentration) in oxygen was used for maintenance of anaesthesia. Lower oesophageal pH was monitored continuously throughout surgery using a pH-measuring probe. Reflux was considered to have occurred whenever pH values of >7.5 (alkaline reflux) or <4 (acid reflux) were recorded. On completion of surgery, carprofen (4 mg kg−1) was administered IV. Further administration of analgesics post-operatively was dictated by visual analogue scale pain scoring.ResultAcid GOR was observed in five of 26 dogs in group D, six of 35 group A, and 12 of 33 group P (p = 0.152). The incidence of GOR in group P approached statistical significance and was higher than the incidence in the combined group A + D (one sided p = 0.044, two sided p = 0.077).ConclusionsIn dogs undergoing ovariohysterectomy, GOR during anaesthesia occurs with a high incidence in dogs in the second half of pregnancy compared to non-pregnant animals during anoestrus or dioestrus.Clinical relevanceMeasures could be taken in such cases to avoid the consequences of potential reflux. 相似文献
32.
Kneknopoulos P Skouroumounis GK Hayasaka Y Taylor DK 《Journal of agricultural and food chemistry》2011,59(3):1005-1011
Anthocyanins and their related compounds were extracted from grape skins of Pinot noir, using 50% aqueous methanol, and purified by solid phase extraction chromatography using XAD-7 resin to obtain a pigment-rich fraction. This fraction was subjected to multilayer coil countercurrent chromatography (MLCCC) using a quaternary solvent system consisting of tert-butyl methyl ether/n-butanol/acetonitrile/water acidified with 0.01% trifluoroacetic acid (2:2:0.1-1.8:5) (v/v/v/v) in a step gradient elution to separate anthocyanin oligomers from grape anthocyanins. In the process of the characterization of the MLCCC fractions by electrospray mass spectrometry, two noncolored anthocyanin derivatives were found and characterized on the basis of their mass spectral data. As a result, these compounds have been tentatively identified as coupling products between both hydrated malvidin-3-glucoside and peonidin-3-glucoside, with 2-S-glutathionyl caffeoyl tartaric acid (GRP). It is therefore proposed that grape skins contain this new class of coupling product, and a possible chemical pathway for their formation is suggested. 相似文献
33.
George N.Zaimes Petros A.Tsioras Chrysovalantis Kiosses Mustafa Tufekcioglu Sergiy Zibtsev Ilya Trombitsky Razvan Uratu Luiza Gevorgyan 《林业研究》2020,31(1):257-268
Protected areas are necessary for the conservation and enhancement of biodiversity.Wildfires are major threats to forests and other natural areas,because they may cause irreversible damages.The aim of this study was to analyze the perspective of experts(N=284),from six countries of the Black Sea,on the current status and problems of protected areas and wildfires.Understanding their points of view could enhance future management on these issues in the region.Data collection was carried out for 9 months,using a web-based questionnaire.Wildfires were perceived as a serious problem in Turkey,Armenia and Greece but as a substantially less serious problem in Romania,Ukraine and Moldova.In Greece,Armenia,and especially Ukraine,the current designated protected areas are considered sufficient to maintain biodiversity,while in Romania,Turkey,and especially Moldova,more areas should be designated as protected.A major need in all countries(except for Turkey)is the increased use of information and communication technologies for both wildfire suppression and protected area management.Experts were divided on whether wildfire suppression and management of protected areas are interconnected.However,there is growing awareness of the adverse impacts of climate change in protected areas and the frequency of wildfires in the future.The most frequently suggested measures to alleviate these impacts were:changes in forest management and increasing public awareness for wildfire suppression,along with changes in forest management and increased staff training to enhance protected area conservation. 相似文献
34.
Charilaos Xiros Evangelos Topakas Petros Katapodis Paul Christakopoulos 《Industrial Crops and Products》2008,28(2):213-224
Brewer's spent grain (BG), the most abundant brewing by-product, is used in the present study as a low-cost feedstock for the production of ethanol by the mesophilic fungus Fusarium oxysporum using a consolidated bioconversion process. The production of required cellulolytic and hemicellulolytic enzymes was optimized under solid-state cultivation (SSC) concerning carbon source and initial moisture. The optimal medium contains BG and corn cobs (CC) in a ratio 7:3 while the optimal initial moisture is 66% (w/w). SSC in a laboratory horizontal bioreactor using the optimized medium allowed the large-scale production of a multienzymic system including endoglucanase, cellobiohydrolase, β-d-glucosidase, xylanase, feruloyl esterase, acetyl esterase, β-d-xylosidase and α-l-arabinofuranosidase. Chromogenic (fluorogenic) 4-methylumbelliferyl substrates were used to partially characterize the extracellular proteome of the microbe after the separation by isolectric focusing (IEF) electrophoresis. Alkali pretreatment of brewer's spent grain and different aeration levels were studied for the optimization of the ethanol production by F. oxysporum in a consecutive submerged fermentation. A yield about 65 g ethanol kg−1 of dry BG was obtained with alkali pretreated BG under microaerobic conditions (0.01 vvm) corresponding to 30% of the theoretical yield based on total glucose and xylose composition of BG. 相似文献
35.
A 3-parameter Boltzman and a 4-parameter Logistic non-linear regression model were constructed to simulate the emergences and seasonal dynamics of the major moth pest complex of peach including Anarsia lineatella (Lepidoptera: Gelechiidae), Grapholitha (Cydia) molesta (Lepidoptera: Tortricidae) and Adoxophyes orana (Lepidoptera: Tortricidae). Model development used 4 yr (2004–2007) climatic data and field observations in peach orchards located in two separate regions in northern Greece. Model performance was evaluated by using the Adjusted r2 and the Akaike and Bayes-Schwartz information criteria. Applied models had a very high prediction capability in most of the cases. The Akaike and Bayes–Schwartz information values of the 4-parameter Logistic model provided better estimates in most data sets. According to the Logistic equation, 50% of the cumulative number of male moths for the 1st, 2nd and 3rd generations of A. lineatella occurred at 151, 785 and 1513 degree-days respectively, for G. molesta at 654, 785 and 1251, and for A. orana at 406, 1260 and 2141 degree-days (Biofix: 1st March in all cases). In addition, a simplified theoretical degree-day model adjustment was attempted to generate simultaneously a multispecies moth phonological model for the above species, in order to outline perspectives in Integrated Pest management (IPM). The unique proposed model revealed differences in the phenology of the three species, and can simplify calculations and recommendations to pest control advisors, based on a common base temperature and biofix. 相似文献
36.
PS Ie D Petros DH Stinner PL Phelan B Hamaker KW Koelling Y Vodovotz 《Journal of agricultural and food chemistry》2012,60(36):9229-9235
The objective of this study was to compare gelatinization properties and molecular composition of starches extracted from locally grown organic and conventional spelt using thermal, rheological, and SEC analyses, along with Concanavalin A method. Organic and conventional spelt was planted in six replicated plots, and the extracted starch was analyzed for their gelatinization properties. DSC showed that the gelatinization temperature ranged from 56.7 to 68.8 °C with an average peak of 62.4 °C, with no evidence for statistical difference in gelatinization properties between treatments. Rheological behavior variation among samples was more pronounced than that between the two growing conditions. The amylose content ranged from 23.0% to 29.8%. There was no significant difference in the molecular weight of amylose and amylopectin irrespective of the plot locations, although a significant difference was found between the amylopectin molecular weight of organic and conventional spelt starches when analyzed collectively. The organic spelt starch studied may substitute the conventional starch when gelatinization behavior is considered. 相似文献
37.
Morphological and free amino acid profile variability,as a tool for stock identification among farmed rainbow trout Oncorhynchus mykiss of different origin 下载免费PDF全文
下载免费PDF全文 Petros V Martsikalis Menelaos Kavouras Georgios A Gkafas Apostolos P Apostolidis Ioannis S Boziaris Athanasios Exadactylos 《Aquaculture Research》2018,49(2):621-630
As a tool for stock identification the body morphology and the free amino acid (FAA) profile variability were determined among farmed rainbow trout Oncorhynchus mykiss (Walbaum 1792) from different geographical areas throughout Greece, using geometric morphometrics and Reversed Phase‐High‐Performance Liquid Chromatography. Significant intraspecific body shape variation was detected among the fish stocks of different origin. Canonical Variate Analysis distinguished populations into two groups being consistent to a great extent with a previous population genetics study. General linear models did not support an association of haplotypes and diet with shape. However, shape was significantly correlated with origin, water temperature, geological‐chemical‐climatic zones, histidine and arginine. Therefore, morphological variation was primarily driven by environmentally induced differences among these zones being the result of phenotypic plasticity. Environmental cues and rearing conditions played the pivotal role in comparison to broodstock origin. Specimens presented different FAA profiles depending on their geographical origin. Principal Component Analysis showed a division between eastern and western Greece which might be owed to the existence of climatic fluctuations affecting the abiotic factors. This combined approach offers an important tool for stock identification thus assisting environmental risk assessments in evaluation of potential ecological effects and fostering research excellence in sustainable aquaculture management. 相似文献