High pressure liquid chromatographic determination of zearalenone in chicken tissues   总被引：1，自引：0，他引：1  

G V Turner  T D Phillips  N D Heidelbaugh  L H Russell 《Journal of the Association of Official Analytical Chemists》1983,66(1):102-104

A method is reported for the extraction and analysis of zearalenone in chicken fat, heart muscle, and kidney tissue by using high pressure liquid chromatography (HPLC). Zearalenone is extracted with acetonitrile, cleaned up with hexane, and extracted further with ethyl acetate. Zearalenone is determined by HPLC using a reverse phase radial compression separation system, an ultraviolet absorbance detector, and a mobile phase of acetonitrile-water (60 + 40) (v/v). Recoveries of zearalenone added at levels from 50 to 200 ng/g are in the range 82.6-95.1%.  相似文献   

Diurnal migration and responses to simulated rainfall in desert soil microarthropods and nematodes     

Walter G. Whitford  Diana W. Freckman  Ned Z. Elkins  Lawrence W. Parker  Rob Parmalee  Janice Phillips  Suzanne Tucker 《Soil biology & biochemistry》1981,13(5):417-425

Diurnal patterns of microarthropod abundance in surface leaf litter were related to its moisture content. Leaf litter moisture was nearly 7% by weight at 0800h but fell to less than 1% by mid-day. Oribatid and tydeid mites moved into litter in the early morning and back into the soil before mid-day. There were no significant differences in numbers of nematodes in litter or soil and 78–98% of the nematodes were anhydrobiotic (coiled) in soil and litter at all times sampled.Following simulated rainfall there were fewer microarthropods in litter at mid-day in the absence of marked decreases in soil and litter moisture content. During drying, there were gradual reductions in numbers and species diversity of litter microarthropods. Nematode numbers did not change as litter dried. Anhydrobiotic nematodes in the soil increased from 14% on day 1 to 85% on day 4. Between 24 and 36 h after simulated rainfall, the proportion of anhydrobiotic litter nematodes increased from 35 to 80%,.Within 1 h after simulated rainfall, there were marked increases in numbers and diversity of microarthropods in surface litter. No collembolans were extracted from dry litter controls but the wet litter was dominated by isotomid, sminthurid and onychiurid collembolans. There were increases in numbers and diversity of oribatid, tydeid and gamasid mites in the wet surface litter within l h after wetting compared to controls.  相似文献   

WILDLIFE CAPTURE COURSE FOR VETERINARIANS          下载免费PDF全文

Dr. Irene Phillips 《The Canadian veterinary journal. La revue veterinaire canadienne》2003,44(3):199

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BILATERAL OVARIAN ADENOCARCINOMA IN A DOG: ULTRASONOGRAPHIC-AIDED DIAGNOSIS     

John-Karl  Goodwin  DVM  David  Hager  DVM  Lesley  Phillips  DVM  Ronald  Lyman  DVM 《Veterinary radiology & ultrasound》1990,31(5):265-267

Abdominal ultrasonography was instrumental in the diagnosis of bilateral ovarian adenocarcinoma in a twelve year old female Irish Setter. Severe ascites limited the diagnostic quality of abdominal radiography, but enhanced ultrasonographic images. Ultrasonographically, the ovarian tumors were large, contained multiple anechoic cystic structures, and had irregular margins. The ultrasonographic appearance of ovarian adenocarcinoma has not been previously reported.  相似文献   

Development of an enzyme-linked immunosorbent assay for the detection and measurement of the trypanocidal drug isometamidium chloride in cattle     

D D Whitelaw  E A Gault  P H Holmes  I A Sutherland  F J Rowell  A Phillips  G M Urquhart 《Research in veterinary science》1991,50(2):185-189

An enzyme-linked immunosorbent assay (ELISA) was developed to measure accurately levels of the trypanocidal drug isometamidium in the serum of treated cattle. The assay requires only 5 microliters of test serum, is sensitive to a level of 0.5 pg ml-1 and is highly specific. Cross reactivity does not occur with the two other widely used trypanocidal drugs diminazene aceturate and homidium bromide. Serum drug levels are detectable for up to six months in cattle after a single dose of 1 mg kg-1 intramuscularly, the maximum period under field conditions for which effective prophylaxis can be maintained against tsetse challenge. Application of the assay will aid the rationalisation of treatment campaigns and assist in assessing the occurrence of drug-resistant trypanosome populations.  相似文献   

Monthly variation in the plasma copper and zinc concentration of pregnant and non-pregnant mares     

D E AUER  J C NG  D P STEELE  A A SEA WRIGHT 《Australian veterinary journal》1988,65(2):61-62

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The effect of assembly and transit stressors on plasma fibrinogen concentration of beef calves.   总被引：2，自引：0，他引：2       下载免费PDF全文

W A Phillips 《Canadian journal of veterinary research》1984,48(1):35-41

Plasma fibrinogen concentration was measured in beef calves at various points within the system presently used to assemble, market and transport calves from one production point to another in order to determine the effect of the stresses encountered. A short haul of 160 km immediately after weaning did not significantly elevate fibrinogen concentration above the pretransit values. Yearling steers transported 400 km and confined in unfamiliar surroundings for 15 h did have an elevated (P less than 0.01) concentration of fibrinogen, but this increase was not significantly different from that of steers which were confined but not transported, thus confinement may be a significant portion of the stress associated with transit. The change in plasma fibrinogen concentration during assembly and transit was dependent upon the farm from which the calves originated. The magnitude of the change in fibrinogen concentration as a result of assembly and transit varied between the years studied. In one year pretransit assembly for ten days resulted in a higher fibrinogen concentration before and after transit than assembly for four days, but no difference was noted between the two groups in the second year. Bovine plasma fibrinogen concentration does increase in response to the stresses associated with assembly and transit. The stress of fasting and housing in unfamiliar surroundings also increase bovine plasma fibrinogen concentration and are present in the assembly and transit system. These two stresses may account for a majority of the stress associated with marketing and transit. The response of beef calves to the marketing and transit system varied between years.  相似文献   

ECG of the month. Atrial fibrillation     

S B Citino  M Bush  L G Phillips 《Journal of the American Veterinary Medical Association》1987,190(8):950-951

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Isolation and characterization of viruses from semen and the reproductive tract of male swine   总被引：2，自引：0，他引：2  

R M Phillips  C W Foley  P D Lukert 《Journal of the American Veterinary Medical Association》1972,161(11):1306-1316

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Effect of salicylates on intestinal secretion in calves given (intestinal loops) Escherichia coli heat-stable enterotoxin     

C M Wise  A P Knight  M J Lucas  C J Morris  R P Ellis  R W Phillips 《American journal of veterinary research》1983,44(12):2221-2225

The inhibitory effect of salicylates on intestinal secretion in 1- to 5-day-old calves given Escherichia coli heat-stable enterotoxin (ST)-induced intestinal fluid response was investigated. Purified ST was diluted in isotonic saline solution to obtain 1:10, 1:25, 1:50, 1:75, and 1:100 dilutions. Each dilution (1 ml) was inoculated into ligated loops in the distal part of the jejunum of each calf. Acetylsalicylic acid (aspirin) given orally (100 mg/kg) at 4 hours before ST was inoculated did not substantially alter the intestinal fluid response to ST. Sodium salicylate (IV) infusion, begun simultaneously when, or at 1 hour after, ST was inoculated, significantly (P less than 0.05) decreased fluid accumulation in those loops inoculated with ST dilutions of 1:25 or greater. The sodium and potassium concentrations of the accumulated fluid did not differ significantly between or within treatment groups. These results indicate that sodium salicylate infusion may be beneficial in treating enterotoxic colibacillosis in calves. Aspirin given orally at the dose used in the present study, would not have any beneficial effect.  相似文献