Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats          下载免费PDF全文

Sui Zhi Tian  Chai Ju Chang  Chih Chi Chiang  Huo Cheng Peh  Mu Chiou Huang  Jai-Wei Lee    Xin Zhao 《Canadian journal of veterinary research》2005,69(1):39-45

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.  相似文献   

投稿启事     

赵万军 《中国工作犬业》2005,(9):48-48

2005年8月28日7时许，内蒙古自治区阿鲁科尔沁旗公安局刑警大队一中队接到报案：8月27日夜间，新民乡某村村民姜某等几家位于村西的约10亩绿豆地里已割下来的绿豆秧被人偷走。  相似文献   

人为影响下古尔班通古特沙漠小尺度生态环境变化   总被引：1，自引：1，他引：1  

钱亦兵  张立运  雷加强  吴兆宁  张锐 《干旱区资源与环境》2003,17(6):82-88

随着沙漠中油气资源勘探开发、公路建设和重大工程相继实施 ,对生态环境的人为影响日益加深。本文以重大工程实验点为例 ,通过研究对比工程影响区内人工沙垄和自然沙垄的砂质新成土理化性状和植被群落特征 ,指出了沙漠生态环境在人为干扰下发生的一系列变化 ,以及受损生态环境在人们采取积极补救措施的情况下所具有的自然修复能力  相似文献   

樟子松在西北干旱沙区的光合日变化特征   总被引：7，自引：0，他引：7  

吴春荣  金红喜  严子柱  满多清  马全林  安富博 《干旱区资源与环境》2003,17(6):144-146

在甘肃省民勤治沙站 ,用 Li- 62 0 0便携式光合仪分别测定人工栽植的 4龄、9龄和 2 0龄樟子松的净光合速率日进程 ,其结果表明 :在干旱沙区 ,9龄以上樟子松的光合速率在午前随光强和气温而上升 ,午后下降。其 4龄、9龄和 2 0龄樟子松的最大净光合速率分别为 8.7μmol· m- 2 · s- 1、62 .3μmol· m- 2 · s- 1、1 9.7μmol· m- 2 · s- 1。  相似文献   

日光温室黄瓜济杂3号的选育   总被引：1，自引：1，他引：1  

孙汉友  赵殿国  于英俊  任国三  杨玉椿 《中国蔬菜》2003,1(4):34-35

母本88001来源于津杂2号,父本88010来源于津研2号与新泰密刺的杂交后代。其一代杂种济杂3号,瓜条顺直,长30～35 cm,单瓜质量约180 g,瓜把短,瓜皮深绿,密瘤白刺,品质优,商品性好,对霜霉病、白粉病、枯萎病抗性强。每667m~2产量7000～14000 kg,适合日光温室越冬茬和早春茬栽培。  相似文献   

毒死蜱和甲氰菊酯对家蚕毒性与安全评价研究   总被引：24，自引：3，他引：24  

吴声敢  王强  赵学平  吴长兴  陈丽萍 《农药科学与管理》2003,24(9):11-14

通过食下毒叶法和接触法测定了毒死蜱和甲氰菊酯对家蚕的毒性。结果表明:食下毒叶法对2龄起蚕的LC50(48h)分别为:毒死蜱2.3322mg/kg桑叶和甲氰菊酯0.1522mg/kg桑叶,其浸渍桑叶的药液浓度分别为4.6622mg/L和0.3044mg/L。接触法中毒死蜱和甲氰菊酯对2龄起蚕的LC50值(24h)分别为:6.32μg/cm2和0.92μg/cm2。毒死蜱和甲氰菊酯的毒性比都<1,对家蚕的毒性为高毒。  相似文献   

三倍体葡萄新品种‘红标无核''   总被引：5，自引：0，他引：5  

赵胜建  郭紫娟  赵淑云  张新忠  周利存 《园艺学报》2003,30(6):758-758

‘红标无核'是以二倍体‘郑州早红'×四倍体‘巨峰'育成的三倍体无核葡萄品种.果实极早熟,比‘巨峰'早熟30 d,果粒大,紫黑色,无核,果肉较脆,品质上等,可溶性固形物含量15.4%,结实力强,丰产,抗病,适应范围广,适宜鲜食和加工罐头.  相似文献   

Characteristics for Practical Use of Attenuated Isolate UA-Fukushima of <Emphasis Type="Italic">Tomato mosaic virus</Emphasis>     

Tsutomu MATSUMOTO  Yuichiro NARA  Hiromitsu FURUYA  Harumi TAKAHASHI  Kiichi TAIRAKO  Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384

L₁₁A-Fukushima (L₁₁A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited. Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L₁₁A-F and L₁₁A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these results, L₁₁A-F appears to possess the properties necessary for practical use. To manage L₁₁A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L₁₁A-F preparations was established. Received 10 June 2002/ Accepted in revised form 30 October 2002  相似文献   

Effects of Latent Infection of Stock Plants and Abundance of Thrips on the Occurrence of <Emphasis Type="Italic">Tomato spotted wilt virus </Emphasis>in Chrysanthemum Fields     

Shohei MATSUURA  Shigeru HOSHINO  Hideaki HAYASHI  Tetsuyuki KOHGUCHI  Kyoji HAGIWARA  Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102

DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV originating from latently infected stock plants in chrysanthemum production fields. Received 27 July 2001/ Accepted in revised form 27 November 2001  相似文献   

腐败组织中毒鼠磷的GC／NPD、GC／ECD检验     

赵敬真  于忠山  王天宇 《农药科学与管理》2002,23(3):16-17

本文初步建立了一套混合溶剂提取、中性层析氧化铝小柱净化及GC／ECD和GC／NPD分析的腐败生物样材中毒鼠磷的检验方法。GC／ECD的检出限为1ng;GC/NPD的检出限为20ng。  相似文献