浅谈县级种子市场管理     

饶汝金 《种子世界》2010,(10):6-7

<正>1目前县级种子市场经营现状1.1种子经营主体多元化《中华人民共和国种子法》(以下简称《种子法》)实施以来,特别是2007年种子体制改革后,种子经营主体迅速增多,分布广,遍布全县各乡镇、村庄。随着种子产业化、品牌化、商业化日趋成熟。销售不再分装的包装种子  相似文献   

抚州市野生色素植物资源及保护利用   总被引：3，自引：0，他引：3  

周亚平  饶军  李江  陈连水 《湖北农业科学》2010,49(10)

经过多年的调查研究,抚州市共有色素植物78种,隶属39科56属。在分析抚州市色素植物资源的基础上,探讨了色素植物资源的合理开发与利用,旨在为抚州市的经济发展提供参考。  相似文献   

甲基红褪色光度法测定数量溴酸根离子     

罗明辉  易碧英  熊劲芳  饶恒  文凌飞 《湖南农业大学学报(自然科学版)》2002,29(2)

研究了盐酸介质中 ,在氯化纳的催化作用下 ,溴酸根离子氧化甲基红褪色的最佳条件 .吸收波长为 5 18nm .表观摩尔吸光系数为 7.2×10 4 L· m ol- 1 · cm- 1 ,溴酸根离子在 3.0～ 18μg/2 5 m L范围内符合比尔定律 .方法用于化学试剂中微量溴酸根的测定 ,结果令人满意 .  相似文献   

TLC法测定虎杖中白藜芦醇的含量   总被引：5，自引：0，他引：5  

周国海  于华忠  李国章  曹庸  饶力群  龙维珍  唐忠海 《湖南林业科技》2005,32(3):11-13

本文建立了虎杖白藜芦醇含量测定的薄层扫描分析方法：以硅胶G为薄层吸附荆，氯仿：丙酮：乙酸：水(4：4：0．5：0．2)为展开剂，在紫外灯(365nm)下观察荧光斑点，在365n／n处并进行扫描测定，线性方程为：Y=(13．826S’10^-6)-0．8698(Y为白藜芦醇浓度ug／ml，S为峰面积)。并对虎杖茎、根、叶进行了含量测定。结果表明：用TIC法测定虎杖中白藜芦醇含量方法简便、快捷、准确度高、重复性好。虎杖鲜根茎中含量达0．548％。  相似文献   

A fine map for maternal lineage analysis by mitochondrial hypervariable region in 12 Chinese goat breeds     

Yan-Ping WU  Wei-Jun GUAN  Qian-Jun ZHAO  Xiao-Hong HE  Ya-Bin PU  Jun-Hong HUO  Jin-Fang XIE  Jian-Lin HAN  Shao-Qi RAO  Yue-Hui MA 《Animal Science Journal》2009,80(4):372-380

As the fast pace of genomic research continues to identify mitochondrial lineages in animals, it has become apparent that many independent studies are needed to support a robust phylogenetic inference. The aim of this study was thus to further characterize the maternal lineage, proposed to originate in southwestern region of China, using a wider survey of diverse goat breeds in China. To this end, we sequenced the mitochondrial hypervariable region 1 (HVR1) of the mtDNA control region in 145 goats of 12 Chinese breeds. Phylogenetic analysis revealed that Chinese goats were classified into four distinct lineages (A, B, C and D) as previously reported. A Mantel test and the analysis of Analysis of Molecular Variance (ANOVA) indicated that there was not an obvious geographic structure among Chinese goat breeds. Population expansion analysis based on mismatch distribution and Fu's Fs statistic indicate that two expansion events in Chinese goats occurred respectively at about 11 and 29 mutational time units ago, revealing two star-like subclades in lineage B corresponding to two population expansion events. Moreover, lineage B sequences were presented only in the breeds of southwestern or surrounding regions of China. Multiple lines of evidence from this study and previous studies indicate that for Chinese goats mtDNA lineage B originated from the southwestern region of China.  相似文献   

Cx43 is involved in electrical remodeling of atrial myocytes through regulating L-type calcium current     

RAO Fang  XUE Yu-mei  DENG Chun-yu  YU Xi-yong  XIAO Ding-zhang  CHEN Shao-xian  LIN Qiu-xiong  YANG Hui  KUANG Su-juan  LIU Xiao-ying  ZHU Jie-ning  WU Shu-lin 《园艺学报》2015,31(11):1986-1991

AIM: To investigate whether the association of connexin 43(Cx43) and L-type calcium channel involved in the pathogenesis of atrial fibrillation (AF). METHODS: The biochemical assays and whole-cell patch-clamp technique were used to study the expression of Cx43 in human atrial tissue. The co-localization of Cx43 and L -type calcium channel, and the regulation of L-type calcium current in atrial myocytes were investigated. RESULTS: The expression of Cx43 at mRNA and protein levels was decreased in human atrial tissues of AF patients. In cultured atrium-derived myocytes (HL-1 cells), knockdown of Cx43 significantly inhibited the mRNA expression of L-type calcium channel α1c subunit, as well as L-type calcium current. Co-localization of Cx43 with L-type calcium channel α1c subunit in mouse atrial myocytes was observed. CONCLUSION: The decrease in Cx43 is involved in the pathogenesis of AF, probably through reducing the L-type calcium current in atrial myoctyes by co-localization with L-type calcium channel, thus representing the potential pathogenesis in atrial fibrillation.  相似文献   

干旱胁迫下斑茅消减文库的构建及分析   总被引：6，自引：0，他引：6  

刘文荣  张积森  饶进  蔡秋华  翁笑艳  阮妙鸿  阙友雄  陈如凯  张木清 《作物学报》2007,33(6):961-967

以干旱胁迫下斑茅(Saccharum arundinaceum Retz.)叶片组织的cDNA为Tester，正常生长条件下斑茅叶片组织cDNA为Driver，利用抑制性消减杂交技术（Suppression Subtractive Hybridization, SSH）构建斑茅干旱胁迫消减文库。文库克隆总量约为30 000个，克隆重组率为99.2%；随机从文库中挑取3500个克隆分析表明，插入片断长度主要集中在200~1 000 bp之间，500 bp以上的有463个克隆，500 bp以下的有3 037个克隆，平均长度约450 bp; 通过随机对16个文库阳性克隆的测序及分析，3个克隆与ATP-NAD kinase、Aldo/keto reductase和锌指蛋白高度同源，3个克隆没有同源性，10个与逆境相关，是功能未知的EST。选择编号为EL0149和EL0145两个克隆进行Northern杂交验证，结果表明EL0149克隆为干旱胁迫上调表达的EST序列，而EL0145在正常供水和干旱条件下均无明显的的杂交信号。说明本研究克隆的SSH文库质量较高，可以进一步利用SSH文库结合芯片表达谱分析解析作物水分胁迫下的相关抗旱基因网络。  相似文献   

小孢子培养获得松花型花椰菜DH再生植株   总被引：7，自引：0，他引：7  

顾宏辉  朱丹华  杨加付  饶立兵  张晓辉 《农业生物技术学报》2007,15(2):301-305

对5个松花型花椰菜(Brassica oleraceavar.botrytisL.)杂种一代的小孢子培养研究表明,小孢子胎胚发生主要依赖于基因型,庆农65天的每花蕾胚状体产量最高,平均达15.5个。松花菜的胚状体萌发率一般在30%左右,并有效地获得了大量的DH再生植株。冷激预处理能显著地影响花椰菜小孢子的胚胎发生,但供体材料间存在不同的结果。结球期和开花结角期再生植株的生育期与育性出现较大分离,可育且能正常结角的比例约占全部小孢子再生植株的50%以上,因而不再需要加倍处理。  相似文献   

Penidiella sp. HEY-1β-葡萄糖苷酶的分离纯化及其酶学性质   总被引：1，自引：0，他引：1  

何友文  李江  王剑锋  饶军  毛慧玲 《林产化学与工业》2011,31(3)

通过Penidiella sp. HEY-1发酵豆渣可制备产胞外β-葡萄糖苷酶(BGL)。粗酶液经过乙醇沉淀、DEAE-Sepharose Fast Flow离子交换层析和Sephadex G-100凝胶过滤层析等步骤后获得了凝胶电泳均一的BGL。通过SDS-PAGE测得其分子质量为65.5 ku。该酶反应的最适温度为60~70℃,最适pH值为3.0,在70℃以下及pH值2.0~8.0范围内均能保持稳定。Mn2+对酶有激活作用,而Na+对酶有抑制作用,其他金属离子对酶的活性影响不大。底物专一性实验表明,该酶可作用于对硝基苯-β-D-葡萄糖苷(p-NPGlu)、邻硝基苯-β-D-半乳糖苷(o-NPG)。作用于p-NPGlu和o-NPG的米氏常数(Km)值分别为0.434和0.411 mmol/L,酶促反应的最大速度(vmax)分别为1.0×10-3和3.3×10-4mol/(L.min)。  相似文献   

华山松种子发芽影响因素和生活力检验方法研究   总被引：1，自引：0，他引：1  

佘佳荣  宋自力  唐强  饶晓辉  刘建兵 《湖南林业科技》2011,38(6):41-42,66

以泾源县沙塘林场提供的华山松种子为试材,对华山松种子发芽的影响因素和生活力检验方法进行了研究.结果表明:纸床培养的华山松种子的发芽率要高于沙床培养的,两者之间存在显著差异;45℃清水浸种72 h的种子比用细纱1～5℃低温层积过的种子萌芽时间和萌芽高峰期明显滞后;染色时间20h、四唑浓度0.5％、染色温度20℃为检验华山...  相似文献