Characterization of enzyme‐producing bacteria isolated from the gut of Asian seabass,Lates calcarifer and milkfish,Chanos chanos and their application for nutrient enrichment of feed ingredients     

Debasis De  Tapas K. Ghoshal  R. Ananda Raja 《Aquaculture Research》2014,45(9):1573-1580

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Reduction of ochratoxin A in broiler serum and tissues by Trichosporon mycotoxinivorans     

Hanif NQ  Muhammad G  Muhammad K  Tahira I  Raja GK 《Research in veterinary science》2012,93(2):795-797

The present study was planned to evaluate the possible transmission of ochratoxin A (OTA) in serum and targeted organs of broilers fed on two levels (500 and 1000 ppb) this toxin in the presence or absence of a toxin deactivator (containing a mycotoxin deactivating yeast Trichosporon mycotoxinivorans) at two inclusion levels (1 and 2 kg/ton of feed) to 270 day-old broiler chicks divided into nine groups (A-I) over a 42 days period. Serum samples were collected at 14, 28 and 42nd day of experiment, whereas, liver and kidney tissues were obtained from broilers slaughtered at 42nd day of experiment. The highest OTA levels were detected in serum, livers and kidneys of OTA treated groups without supplementation of toxin deactivator (groups D and G) at day 42 of experiment, while the residues were significantly (P<0.01) lower in treatment groups (F and I) supplemented with toxin deactivator at 2 kg/ton of feed. The order of OTA level was serum>kidneys>liver.  相似文献   

Radiographic assessment of pulmonary fluid clearance and lung aeration in newborn calves delivered by elective Caesarean section          下载免费PDF全文

MK Abdelmegeid  O Kutasi  MN Nassiff  K Wirth  E Bodai  F Farrag  L Molnár  O Szenci 《Reproduction in domestic animals》2017,52(6):939-944

The aim of this study was to develop a radiographic standard for the assessment of pulmonary fluid clearance and lung aeration in newborn calves. Caesarean‐delivered mature calves (n = 9) underwent lung assessment by thoracic radiography as well as arterial and venous blood gas analysis within the first 30 min, 1, 2, 3, 6, 12 and 24 hr after birth. The results indicated that newborn calves delivered by elective Caesarean section suffered from a physiological combined respiratory and metabolic acidosis with the dominance of respiratory acidosis, and an improvement in these conditions was recorded within 24 hr after birth. Concerning the radiographic results, clear lung fields, improvement in lung expansion, air content of the lung and absence of lung opacification occurred within 24 hr of birth. Furthermore, the ventral lung quadrant showed an improvement in radiographic opacification and lung expansion earlier than the dorsal lung regions. The findings of this study support the potential role of thoracic radiography in the assessment of pulmonary fluid clearance and lung aeration in newborn calves.  相似文献   

Genetic diversity and phylogeny of pink-pigmented facultative methylotrophic bacteria isolated from the phyllosphere of tropical crop plants     

P. Raja  D. Balachandar  S. P. Sundaram 《Biology and Fertility of Soils》2008,45(1):45-53

Genetic diversity of pink-pigmented facultative methylotrophic bacteria belonging to the genus, Methylobacterium, was assessed using 16S ribosomal RNA (rRNA) gene sequencing, amplified ribosomal DNA restriction analysis (ARDRA), and differential carbon-substrate utilization profile in the phyllosphere of cotton, maize, sunflower, soybean, and mentha plants. Methylobacterium populi, Methylobacterium thiocyanatum, Methylobacterium suomiense, M. aminovorans, and Methylobacterium fujisawaense were identified to colonize the phyllosphere of these crop plants. Among these, M. populi found to be the dominating species followed by M. aminovorans. The diversity indices like Shannon index of diversity, Pielou index of evenness, and Margalef index of richness calculated based on combined data of ARDRA, 16S rRNA gene sequence, and differential carbon-substrate utilization revealed that sunflower leaves showed richest methylobacterial diversity, followed by soybean, while cotton and mentha leaves recorded the lowest diversity. The outcome of this study indicates that diversity of Methylobacterium in phyllosphere depends upon the host plant species colonized.  相似文献   

Susceptibility of betanodavirus in a newly established vertebra-derived cell line from Mosquitofish (Gambusia affinis)     

Jyotsna  Parameswaran Vijayakumar  Ravi Mani  Sudhakaran Raja  Tharmathass Stalin Dhas 《Journal of fish diseases》2020,43(2):263-273

In the present study, a new cell line from the vertebra of mosquitofish Gambusia affinis was successfully established and characterized. The cell line is named as bone Gambusia affinis (BGA) and subcultured for more than 55 passages in Leibovitz's/L15 medium supplemented with 15% FBS at 28°C. The cell line has a modal chromosome number of 48. Molecular characterization of the partial sequence of the coi gene confirmed the origin of the BGA cell line from mosquitofish. These cells exhibited epithelial morphology confirmed by the cytokeratin marker. The BGA cells showed mineralization of their extracellular matrix when stained with alizarin red and von Kossa stain. BGA cells were found to be susceptible to RGNNV and SJNNV strains of betanodavirus (NNV) showing cytopathic effect with multiple vacuolations in the cells. The RT-PCR confirmed the betanodavirus infections in BGA cells. The SEM micrograph showed the morphological changes observed in the cell during virus infection. The in vivo challenge experiment also showed the viral replicating efficiency in the Gambusia affinis with increasing viral titre. Thus, our present results show that the BGA cell line is a useful tool for isolating betanodavirus and could be used to investigate bone cell differentiation and extracellular matrix mineralization.  相似文献   

Development,characterization, conservation and storage of fish cell lines: a review   总被引：1，自引：0，他引：1  

W. S. Lakra  T. Raja Swaminathan  K. P. Joy 《Fish physiology and biochemistry》2011,37(1):1-20

Cell lines provide an important biological tool for carrying out investigations into physiology, virology, toxicology, carcinogenesis and transgenics. Teleost fish cell lines have been developed from a broad range of tissues such as ovary, fin, swim bladder, heart, spleen, liver, eye muscle, vertebrae, brain, skin. One hundred and twenty-four new fish cell lines from different fish species ranging from grouper to eel have been reported since the last review by Fryer and Lannan (J Tissue Culture Methods 16: 87–94, 1994). Among the cell lines listed, more than 60% were established from species from Asia, which contributes more than 80% of total fish production. This includes 59 cell lines from 19 freshwater, 54 from 22 marine and 11 from 3 brackish water fishes. Presently, about 283 cell lines have been established from finfish around the world. In addition to the listing and a scientific update on new cell lines, the importance of authentication, applications, cross-contamination and implications of overpassaged cell lines has also been discussed in this comprehensive review. The authors feel that the review will serve an updated database for beginners and established researchers in the field of fish cell line research and development.  相似文献   

An unusual outbreak of contagious ovine ecthyma   总被引：1，自引：0，他引：1  

CD HAWKINS  TM ELLIS  MK DAVIES  RL PEET  J. PARKINSON 《Australian veterinary journal》1991,68(6):210-211

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Classification of soils supporting mangrove plantation in Kuwait     

NR Bhat  MK Suleiman 《Archives of Agronomy and Soil Science》2013,59(6):535-551

The State of Kuwait has initiated an ambitious national program to establish greenery in 20000 ha of open spaces in inland and coastal areas. Afforestation of intertidal zones with mangrove plants is being considered as a viable option for greening coastal areas. In view of this fact, experimental plantations were established using two ecotypes of Avicennia marina (Bahrain and UAE) and one ecotype of Avicennia germinans (Florida). The study consisted of two 10-year old plantations and two 2-year old plantations. Seedlings established successfully and grew vigorously on some of the locations within the site. Therefore, studies were conducted during 2002-2003 to describe and classify soils that supported maximum mangrove establishment and growth under the harsh arid coastal conditions of Kuwait. For this purpose, eight soil profiles (four each representing areas where seedlings established successfully and grew vigorously and where all seedlings died in the initial stages of establishment) were investigated and classified according to the soil taxonomy. The main soil types observed in the study site were: Typic Aquisalids, Typic/sodic Aquicambids and Calcic Aquisalids. Mangrove growth performance in relation to the landform, soil type and properties of the soil at various depths is discussed in the paper. The plant growth appeared to be the better on the typic Aquisalid soil than that on the Typic/sodic Aquicambid soils. The relative proportions of the coarse sand fraction, occurrence of the anaerobic layer in the profile and the surface layer salinity influenced both the establishment and growth of mangrove plants.  相似文献   

Cold Plasma: A Potential Alternative for Rice Grain Postharvest Treatment Management in Malaysia     

Mohd Fadthul Ikmal Misnal  Norizah Redzuan  Muhamad Nor Firdaus Zainal  Norhayati Ahmad  Raja Kamarulzaman Raja Ibrahim  Linda Agun 《水稻科学》2022,29(1):1-15

Use of pesticides, herbicides and fertilizers is among the techniques to control insect pests and fungal pathogens. However, the technique is the major contributor to severe environmental implications in terms of air, water and soil pollution. Besides, variable inconsistency becomes an important issue in the implementation of inclined bed dryers, leading to significant rice grain loss. Cold plasma technology has been widely proposed as a potential alternative for rice grain postharvest treatment management due to the presence of generated ionised gas that eventually produces reactive oxygen species or reactive nitrogen species. These species are used to decontaminate foodborne pathogens, mycotoxins and bacterial diseases. This review explores the current literature regarding cold plasma treatment technology, focusing on its efficiency as the microbial decontamination medium and insect pest mortality medium, and on the enhancement functional, nutritional and cooking properties, especially in rice grains. Previous studies have successfully demonstrated the ability of cold plasma treatment to significantly reduce the microbial count of foodborne pathogens, detoxify mycotoxins, and control seedborne rice seedling bacterial diseases. Previous studies have also proved that the implementation of cold plasma technology in postharvest management should be seriously considered for improving rice grain quantity and quality in Malaysia.  相似文献   

Effect of Artificial Insemination Protocol and Dose of Frozen/Thawed Stallion Semen on Pregnancy Results in Mares     

JLJ Govaere  MK Hoogewijs  C De Schauwer  S De Vliegher  A Van Soom  L Duchateau  A de Kruif 《Reproduction in domestic animals》2014,49(3):487-491

Deep intra‐uterine insemination is commonly accepted as a routine procedure for artificial insemination in horses. The motives and principles of deep insemination are well described, but the equipment used may differ. In this trial, the efficiency of two different insemination pipettes for deep intra‐uterine insemination in the mare was compared with insemination into the uterine body using commercially available frozen–thawed semen of two stallions of proven fertility. These inseminations were performed using two different doses. The semi‐flexible Minitube pipette was compared with a newly designed insemination device with a more flexible telescopic insemination catheter (Ghent device). The semi‐flexible Minitube pipette performed better than the newly designed insemination device with respect to pregnancy outcome (p = 0.008). The superiority of deep horn insemination over uterine body insemination was reflected by the better pregnancy rates obtained after deep insemination using the same low doses (30.6% better pregnancy rates) (p = 0.0123).  相似文献