Potentially toxic elements in the riparian soils of the Sava River     

Milica Marković  Tea Zuliani  Snežana Belanović Simić  Zorana Mataruga  Olga Kostić  Snežana Jarić  Janja Vidmar  Radmila Milačič  Janez Ščančar  Miroslava Mitrović  Pavle Pavlović 《Journal of Soils and Sediments》2018,18(12):3404-3414

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Multilocus PCR assay reveals high diversity of vegetative compatibility types in populations of Cryphonectria parasitica in Croatia          下载免费PDF全文

J. Mlinarec  M. Ježić  J. Ćosić  M. Ćurković‐Perica 《Plant pathology》2018,67(3):741-749

The ascomycete fungus Cryphonectria parasitica, causal agent of chestnut blight, is probably one of the best known invasive fungal pathogens in forests of Europe and North America. Mycovirus that reduces virulence of C. parasitica can be used as a biocontrol agent of the chestnut blight. However, anastomosis‐mediated virus transmission is limited by a vegetative (in)compatibility (vc) system involving at least six known diallelic vic genetic loci. This study looked at vegetative compatibility (vc) diversity in two populations of C. parasitica in Croatia. For that purpose, a PCR assay was validated and implemented using already known/published and newly designed primers for amplification of six known vic loci. The vc genotypes determined by PCR for 158 C. parasitica isolates investigated in this study were in complete agreement with the vc genotypes determined by pairwise co‐culturing of the same isolates, revealing the specificity and accuracy of the PCR‐based molecular vic genotyping assay. Twenty‐six unique vc genotypes were found among 158 isolates, and 19 vc types per population, which makes Croatian C. parasitica populations among the most diverse in Europe regarding the number of vc types and genetic diversity. Low values of multilocus linkage disequilibrium suggest sexual reproduction as a major contributor to high C. parasitica genetic diversity in studied populations.  相似文献   

Comparative Analysis of Native <Emphasis Type="Italic">Crocus</Emphasis> Taxa as a Great Source of Flavonoids with High Antioxidant Activity     

Ivana Šola  Mirta Stipaničev  Valerija Vujčić  Božena Mitić  Ana Huđek  Gordana Rusak 《Plant foods for human nutrition (Dordrecht, Netherlands)》2018,73(3):189-195

Native Crocus taxa are abundant in regions with moderate and dry climate; however, their potential in the food industry has thus far been neglected. The objective of this study was to compare the quantitative and qualitative flavonoid content and antioxidant and cytotoxic activity in organs of several populations of Croatian native taxa and assess their potential for application in the food industry. The dominant flavonoids in the native Crocus taxa were kaempferol and quercetin. Tepals contained similar or higher concentrations of flavonoid-glycosides than other organs. Tepals from Cvv1 and Cvv2 populations contained more flavonoid-glycosides than the commonly used spice saffron (C. sativus stigmas). The FRAP antioxidant activity of Cvv1 and Cvv5 tepals was similar to that of standard Trolox. DPPH inhibition of Cvv1 and Cvv3 tepals was within the range of that recorded for saffron. Cvv1 tepals significantly reduced reactive oxygen species in the broadest concentration range (50–1000 μg/ml), and showed considerable antioxidant activity in the ABTS assay, equal to 82% of standard Trolox antioxidant activity. A significantly higher concentration of kaempferol-rutinoside was recorded in this than in other taxa. The flavonoid showed a very strong or strong correlation with antioxidant assays results, and a negative correlation with cellular reactive oxygen species concentration. We therefore presumed that kaempferol-rutinoside is one of the main antioxidant phenolics in Crocus tepals. None of the tested extracts showed cytotoxicity toward Caco-2 cells. The results revealed that Cvv tepals have potential as a food supplement and are a promising material for further food safety tests.  相似文献   

How Selenium Affects Berula erecta     

Špela Mechora  Metka Sotler  Andreja Urbanek Krajnc  Jana Ambrožič-Dolinšek 《Water, air, and soil pollution》2016,227(12):451

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A case study of FD and BN phytoplasma variability in Croatia: multigene sequence analysis approach     

Plavec  Jelena  Križanac  Ivana  Budinšćak  Željko  Škorić  Dijana  Musić  Martina Šeruga 《European journal of plant pathology / European Foundation for Plant Pathology》2015,142(3):591-601

European Journal of Plant Pathology - Uncultivable bacteria from the genus ‘Candidatus Phytoplasma’ are associated with grapevine yellows (GY) diseases worldwide. In Euro-Mediterranean...  相似文献   

Degradation Kinetics of an Aged Hydrocarbon-Contaminated Soil   总被引：1，自引：0，他引：1  

Snežana Maletić  Božo Dalmacija  Srđan Rončević  Jasmina Agbaba  Olgica Petrović 《Water, air, and soil pollution》2009,201(1-4):149-159

PM2.5 and PM10 samples were collected in the urban atmosphere of Elche (southeastern Spain) between December 2004 and November 2005. The samples were analyzed for mass and water-soluble inorganic ions (Na⁺, ${\text{NH}}^{{\text{ + }}}_{{\text{4}}}$ , K⁺, Ca²⁺, Mg²⁺, Cl^?, ${\text{NO}}^{{\text{ - }}}_{{\text{3}}}$ and ${\text{SO}}^{{{\text{2 - }}}}_{{\text{4}}}$ ) with the aim of investigating the influence of the climatic and geographic features of a coastal semiarid area on the contribution of these species to PM levels. Secondary inorganic ions ( ${\text{SO}}^{{{\text{2 - }}}}_{{\text{4}}}$ , ${\text{NO}}^{{\text{ - }}}_{{\text{3}}}$ , ${\text{NH}}^{{\text{ + }}}_{{\text{4}}}$ ) were the major components in the fine fraction (PM2.5), accounting for 40% of the total mass. The relationship between non-marine ${\text{SO}}^{{{\text{2 - }}}}_{{\text{4}}}$ and ${\text{NH}}^{{\text{ + }}}_{{\text{4}}}$ indicated that fine sulfate particles were completely neutralized by ammonium. In the coarse fraction (PM10–2.5), nitrate (as NaNO₃ and Ca(NO₃)₂), together with crustal (CaCO₃) and marine species (NaCl) accounted for almost 50% of the total mass. Fine sulfate and coarse nitrate showed summer maximums. In contrast, the concentrations of fine ${\text{NO}}^{{\text{ - }}}_{{\text{3}}}$ were lowest in the warm period. Ammonium presented both winter and summer maximums. The levels of marine ions, except for coarse Cl^?, were highest in summer when the dominant wind flow is from the sea. No significant seasonal variations were observed for coarse Ca²⁺ and ${\text{SO}}^{{{\text{2 - }}}}_{{\text{4}}}$ . The concentrations of all inorganic ions increased during Saharan dust events, in particular, fine ${\text{NH}}^{{\text{ + }}}_{{\text{4}}}$ and ${\text{SO}}^{{{\text{2 - }}}}_{{\text{4}}}$ and coarse $NO_3^ - $ . Coarse calcium was proved not to be a good tracer for this type of episode in our region since the average levels of this cation are elevated and the relative increase in its concentrations during African events was not as high as expected.  相似文献   

Alkaline Comet Assay as a Potential Tool in the Assessment of DNA Integrity in Freshwater Zooplankton Affected by Pollutants from Water Treatment Facility     

Ternjej  Ivančica  Stanković  Igor  Mihaljević  Zlatko  Furač  Lidija  Želježić  Davor  Kopjar  Nevenka 《Water, air, and soil pollution》2009,204(1-4):299-314

Water, Air, & Soil Pollution - In this pilot study, we evaluated the usefulness of the alkaline comet assay as a biomonitoring tool at the level of plankton communities affected by varying...  相似文献   

Influence of wildfire severity on riparian plant community heterogeneity in an Idaho,USA wilderness     

Breeanne K. Jackson  S. Mažeika P. Sullivan 《Forest Ecology and Management》2009

Despite the increasing recognition of riparian zones as important ecotones that link terrestrial and aquatic ecosystems and of fire as a critical natural disturbance, much remains unknown regarding the influence of fire on stream-riparian ecosystems. To further this understanding, we evaluated the effects of mixed severity wildfire on riparian plant community structure and composition in headwater streams of the Big Creek Watershed of the Frank Church ‘River of No Return’ Wilderness of central Idaho. Five years after a large stand-replacing fire, we conducted riparian vegetation surveys at sixteen reaches across a range of burn types. Non-metric Multidimensional Scaling (NMS) and Multi-Response Permutation Procedure (MRPP) analyses showed an overall shift in community composition and structure between vegetation at unburned and severely burned reaches. Although total plant cover was significantly less at severely burned areas, recovery of the deciduous understory was apparent. Severely burned reaches were characterized by a marked increase in cheatgrass (Bromus tectorum). Reaches that were exposed to low-severity fire were indistinguishable from unburned reaches relative to vegetation community composition and structure, pointing to a possible disturbance threshold that may need to be crossed in order to alter riparian plant communities.  相似文献   

Glutelin protein fraction as a tool for clear identification of Amaranth accessions     

Mária Džunková  Dagmar Janovská  Petra Hlásná Čepková  Anna Prohasková  Michal Kolář 《Journal of Cereal Science》2011

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Occurrence and pathogenicity of Phytophthora × cambivora on Prunus laurocerasus in Serbia          下载免费PDF全文

I. Milenković  N. Keča  D. Karadžić  Z. Radulović  M. Tomšovský  T. Jung 《Forest Pathology》2018,48(4)

Cherry laurel (Prunus laurocerasus) is a native plant species in Serbian forests, but is also widely used for ornamental plantings. Following two extremely wet summers in 2014 and 2015, in spring and summer of 2016 and 2017, numerous cherry laurel plants with symptoms indicative for Phytophthora diseases, like wilting and chlorosis of leaves, dieback and bleeding bark necroses, were recorded in a park in Belgrade and in two ornamental nurseries in central Serbia. From necrotic bark samples and rhizosphere soil, self‐sterile Phytophthora isolates with woolly colonies were obtained. Due to the production of ellipsoid and elongated, non‐papillate sporangia in water and of ornamented oogonia with two‐celled antheridia in mating tests with tester strains of both Phytophthora × cambivora and P. cryptogea, these isolates were identified as P. ×cambivora which was confirmed by ITS sequence analysis. Pathogenicity of P. ×cambivora from cherry laurel (PCCL) was tested by inoculating one‐year‐old seedlings of cherry laurel under the bark. P. ×cambivora from European beech (PCB), and isolates of P. cactorum (CAC), P. cryptogea (CRY), P. plurivora (PLU) and P. ×serendipita (SER) were included as comparison. Three and a half months after inoculation, nine of the twelve plants in PCB, three in PCCL and CAC and two in PLU declined with longitudinal necroses and chlorosis, wilting and premature shedding of leaves. These results demonstrate the ability of P. ×cambivora to infect and cause decline of cherry laurel plants. The particularly high aggressiveness of the P. ×cambivora isolate from beech shows that this pathogen poses a serious risk to cherry laurel in the rare natural communities of cherry laurel and beech in Serbia.  相似文献