Pigeonpea improvement: An amalgam of breeding and genomic research     

Sameer Kumar Chanda Venkata  Ganga Rao Nadigatla Veera Prabha Rama  Rachit K. Saxena  Kulbhushan Saxena  Hari D. Upadhyaya  Moses Siambi  Said N. Silim  Kothapally Narasimha Reddy  Anupama J. Hingane  Mamta Sharma  Shivali Sharma  Stephen Dominic Lyimo  Rose Ubwe  Meshack Makenge  Kananji Gad  Paul Kiprotich Kimurto  Manuel Amane  Kennedy Kanenga  Yuventino Obong  Emanuel Monyo  Chris Ojiewo  Nagesh Kumar Mallela Venkata  Jaganmohan Polineni Rao  Prashanthi Lakkireddy  Sudhakar Chourat  Indraprakash Singh  Sobhan Sajja  Shruthi Hirikara Beliappa  Rajeev K. Varshney 《Plant Breeding》2019,138(4):445-454

In the past five decades, constant research has been directed towards yield improvement in pigeonpea resulting in the deployment of several commercially acceptable cultivars in India. Though, the genesis of hybrid technology, the biggest breakthrough, enigma of stagnant productivity still remains unsolved. To sort this productivity disparity, genomic research along with conventional breeding was successfully initiated at ICRISAT. It endowed ample genomic resource providing insight in the pigeonpea genome combating production constraints in a precise and speedy manner. The availability of the draft genome sequence with a large‐scale marker resource, oriented the research towards trait mapping for flowering time, determinacy, fertility restoration, yield attributing traits and photo‐insensitivity. Defined core and mini‐core collection, still eased the pigeonpea breeding being accessible for existing genetic diversity and developing stress resistance. Modern genomic tools like next‐generation sequencing, genome‐wide selection helping in the appraisal of selection efficiency is leading towards next‐generation breeding, an awaited milestone in pigeonpea genetic enhancement. This paper emphasizes the ongoing genetic improvement in pigeonpea with an amalgam of conventional breeding as well as genomic research.  相似文献   

Exploring diversity among rice germplasms based on their physiological traits responses to salinity     

Inja Naga Bheema Lingeswar Reddy  Suk-Young Lee  Yong-Jin Park  Taek-Ryoun Kwon 《Journal of Crop Science and Biotechnology》2017,20(2):137-152

Salinity is one of the major abiotic stresses that severely effects rice production throughout the world. Previously, there have not been many studies which focused on studying diversity among rice germplasm based on specific physiological traits for salt tolerance. Our diversity study was based on physiological traits such as Na+ concentration, K⁺ concentration, K⁺/Na⁺ ratio, osmotic potential, and biomass which are major components determining salt tolerance. This study has systematically analyzed phenotypic data of 191 germplasms in two different salt concentrations apart from the control. The current study identified salt tolerant germplasms based on their response to a single physiological trait as well as a combination of different physiological traits. Some of the germplasm identified outperformed known salt tolerant cultivar Pokkali. This study identifies correlation among various physiological traits. The salt tolerant germplasms can be taken forward into developing better varieties by conventional breeding and exploring genes for salt tolerance.  相似文献   

Seasonal Effect on Zebu Embryo Quality as Determined by Their Degree of Apoptosis and Resistance to Cryopreservation     

YC Marquez  CS Galina  N Moreno  H Ruiz  A Ruiz  H Merchant 《Reproduction in domestic animals》2005,40(6):553-558

In order to optimize the production of embryos under tropical conditions and to test a possible seasonal effect on embryo quality, 40 Zebu cows were superovulated during the dry season (April to May) and during the rainy season (July to August). A total of 116 (average 2.7/cow) and 83 embryos (3.5 average/cow) were obtained during the respective seasons. After classification as good, fair or poor quality, embryos were tested based on their ultrastructural differences (n = 53 dry season 16 good, 20 fair and 17 poor and n = 61 rainy season 21 good, 20 fair and 20 poor) and their degree of apoptosis using the TUNEL technique (n = 30 during the dry season and n = 55 in the rainy season). Structural characteristics determining embryo quality varied between good and fair quality embryos. No difference, however, was observed between good, fair and poor quality embryos from the two seasons. The number of TUNEL-positive cells was different among embryos (p < 0.001), being lower in labelled cells of good quality embryos regardless of the season. Fewer apoptotic cells were observed in embryos assigned in all three quality levels during the rainy season (p < 0.001). Ultrastructural evaluations confirmed the results obtained by TUNEL. Cryopreserved embryos of good (n = 25 in each season) and fair quality (n = 11 dry season; n = 17 rainy season) showed a significant decrease of TUNEL-positive cells during the rainy season (p < 0.05). Results suggest that embryos collected in the dry season have more cellular damage in contrast; embryos cryopreserved in the rainy season appeared morphologically better equipped to result in a pregnancy following transfer.  相似文献   

Intestinal obstruction in a she-buffalo-a case report     

O Ramakrishna  S K Reddy  J S Reddy 《The Indian veterinary journal》1971,48(6):650-652

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Studies on the bacterial contamination of market eggs--a preliminary report     

P C Panda  B Panda  M S Reddy 《The Indian veterinary journal》1968,45(5):439-443

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Evapotranspiration relationship with pan evaporation and evapotranspiration ratio of corn under different nitrogen levels and moisture regimes     

M Devender Reddy  I Krishnamurthy  K Anand Reddy  A Venkatachari 《Agricultural Water Management》1981,3(3):227-231

In a field experiment with four moisture regimes and eight nitrogen levels, the ratios between evapotranspiration and pan evaporation ($\text{Et}\text{Eo}$) were low in the initial stages of crop growth and attained maximum values at 70–80% (20 and 40% available soil moisture depletion (ASMD)), 65% (60% ASMD) and 55% (80% ASMD) of the crop growth stage. Amongst nitrogen levels, the evapotranspiration ratio (ETR) was highest (3573) under no nitrogen and lowest (1312) with 180 kg N/ha. The 20% ASMD regime utilised less water (ETR= 1499 to produce a kilogram of grain than did the other moisture regimes. The lowest evapotranspiration ratio (914) was recorded with 20% ASMD and 180 kg N/ha in combination. The highest ETR (3954) was found with 60% ASMD and no nitrogen. An additive effect of nitrogen and moisture was found, in indicating that they can be substituted one for the other, when one of them becomes a constraint.  相似文献   

A rare case of uterine rupture and escape of conceptus into the abdominal cavity in a pre-parturient buffalo-cow     

D B Reddy  N Khan  G Yadgirkar 《The Veterinary record》1966,79(19):540-541

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Rhizobacteria-Mediated Growth Promotion of Tomato Leads to Protection Against Cucumber mosaic virus     

Murphy JF  Reddy MS  Ryu CM  Kloepper JW  Li R 《Phytopathology》2003,93(10):1301-1307

ABSTRACT We evaluated combinations of two strains of plant growth-promoting rhizobacteria (PGPR) formulated with the carrier chitosan for the ability to induce growth promotion of tomato plants and resistance to infection by Cucumber mosaic virus (CMV). Each PGPR combination included GB03 (Bacillus subtilis) and one of the following PGPR strains: SE34 (B. pumilus), IN937a (B. amyloliquefaciens), IN937b (B. subtilis), INR7 (B. pumilus), or T4 (B. pumilus). The PGPR combinations formulated with chitosan are referred to as biopreparations. Tomato plants treated with each of the biopreparations appeared phenotypically and developmentally similar to nonbacterized control plants that were 10 days older (referred to as the older control). When plants were challenged with CMV, all plants in the biopreparation treatments and the older control treatment had significantly greater height, fresh weight, and flower and fruit numbers than that of plants in the CMV-inoculated same age control treatment. CMV disease severity ratings were significantly lower for biopreparation-treated and older control tomato plants than for that of same age control plants at 14 and 28 days postinoculation (dpi). CMV accumulation in young noninoculated leaves was significantly less for all biopreparation-treated plants and those in the older control than for the same age control plants at 14 dpi and for four of the five biopreparation treatments at 28 dpi. In those tomato plants shown to be infected, the amount of CMV in noninoculated leaves was significantly lower for three of the biopreparation treatments and the older control treatment at 14 dpi and biopreparation G/INR7 treatment at 28 dpi when compared with the control treatment. These data show that treatment of tomato plants with biopreparations results in significant enhancement of growth and protection against infection by CMV.  相似文献   

Relative bio-availability and utilisation of phosphatic fertilisers as sources of phosphorus in broilers and layers     

Rama Rao SV  Ramasubba Reddy V 《British poultry science》2003,44(1):96-103

1. Different concentrations of non-phytate phosphorus (NPP, 2.5, 3.0, 3.5, 4.0 and 4.5 g/kg diet) were given to broilers (8 to 42 d of age) to establish regressions between dietary NPP concentration and body weight gain and tibia ash content. Second and third experiments were conducted to study the feasibility of utilisation of different phosphatic fertilisers [ammonium phosphate (AP), ammonium polyphosphate (APP), single super phosphate (SSP), NPK (17:17:17, NPK) and NP (28:28:0, NPK)] in commercial broilers (8 to 42 d) and White Leghorn layers (252 to 364 d). 2. Phosphatic fertilisers were incorporated both in broiler (10 g calcium and 4.5 g NPP/kg) and layer (35 g calcium and 3.5 g NPP/kg) diets by replacing dicalcium phosphate (DCP) in toto. 3. The logarithmic curves obtained for predicting the body weight gain and tibia ash content at different levels of NPP used in experiment 1 were Y = 156.27 + 2,468.8 logX (r2= 0.958) and Y = 530.82 + 144.26 log X (r2 = 0.916), respectively. 4. Body weight gain and food intake in broilers given APP- or NP-supplemented diets were comparable to these in the DCP-fed group. Feeding of NPK, AP or SSP resulted in significant depression in weight gain and food intake and high excreta moisture content. Food/gain, Ca and P contents in tibia ash and serum were not influenced by the use of phosphatic fertilisers as P sources in broiler diets. 5. Tibia ash content in broilers fed on diets containing fertilisers was either similar to or significantly higher than that in the DCP-fed group. Broilers on AP or SSP retained more P and had higher tibia ash content than those on DCP. AP, SSP or NPK caused degenerative and necrotic changes in liver, kidney and intestine of broilers. 6. Relative bio-availability of P from APP or NP was better for body weight gain than AP, SSP or NPK, while the reverse was true for bone calcification. 7. APP and NP gave hen-d egg production similar to that of DCP-fed layers. Food intake was significantly reduced in layers fed on diets containing fertilisers. However, food/egg mass, egg weight and serum Ca and inorganic P contents were not influenced by inclusion of fertilisers in layer diets. 8. Except for AP, inclusion of fertilisers in layer diets reduced shell weight and shell thickness compared with the DCP-fed group. However, no apparent eggshell defects were found which could be attributable to diet. 9. Results of these experiments suggest that APP and NP can be used as the sole source of P both in broiler and layer diets, replacing DCP in toto. However, when utilising these P sources in layers, due attention should be given to shell quality. Fertilisers containing high F (AP and SSP) or K (NPK) reduced performance in broilers and layers and caused microscopic changes in liver, kidney and intestine in broilers.  相似文献   

Induced systemic protection against tomato late blight elicited by plant growth-promoting rhizobacteria     

Yan Z  Reddy MS  Ryu CM  McInroy JA  Wilson M  Kloepper JW 《Phytopathology》2002,92(12):1329-1333

ABSTRACT Two strains of plant growth-promoting rhizobacteria (PGPR), Bacillus pumilus SE34 and Pseudomonas fluorescens 89B61, elicited systemic protection against late blight on tomato and reduced disease severity by a level equivalent to systemic acquired resistance induced by Phytophthora infestans or induced local resistance by chemical inducer beta-amino butyric acid (BABA) in greenhouse assays. Germination of sporangia and zoospores of P. infestans on leaf surfaces of tomato plants treated with the two PGPR strains, pathogen, and chemical BABA was significantly reduced compared with the noninduced control. Induced protection elicited by PGPR, pathogen, and BABA were examined to determine the signal transduction pathways in three tomato lines: salicylic acid (SA)-hydroxylase transgenic tomato (nahG), ethylene insensitive mutants (Nr/Nr), and jasmonic acid insensitive mutants (def1). Results suggest that induced protection elicited by both bacilli and pseudomonad PGPR strains was SA-independent but ethylene- and jasmonic acid-dependent, whereas systemic acquired resistance elicited by the pathogen and induced local resistance by BABA were SA-dependent. The lack of colonization of tomato leaves by strain 89B61 suggests that the observed induced systemic resistance (ISR) was due to systemic protection by strain 89B61 and not attributable to a direct interaction between pathogen and biological control agent. Although strain SE34 was detected on tomato leaves, ISR mainly accounted for the systemic protection with this strain.  相似文献