Tetraploid and hexaploid wheats express identical isoforms of nsLTP1     

Capocchi A  Fontanini D  Muccilli V  Cunsolo V  Saviozzi F  Saletti R  Foti S  Galleschi L 《Journal of agricultural and food chemistry》2006,54(6):2372-2377

Nonspecific lipid-transfer proteins (nsLTPs) have been recognized as allergens in several plant species among which are cereals important in human nutrition. In this report, we purified a 9600 +/- 1 Da protein from both soft wheat and farro bran. Mass spectrometric analyses revealed that these proteins are identical, belong to the nsLTP1 class, and have high sequence homology with nsLTP1 isolated from other cereal species. Their identification was further supported by the ability of the soft wheat nsLTP1 to transfer pyrene-labeled lipids between donor and acceptor membranes. The results are discussed in view of the increasing diffusion on the markets of bran-rich products.  相似文献   

Residue levels and storage responses of nectarines, apricots, and peaches after dip treatments with fludioxonil fungicide mixtures     

D'Aquino S  Schirra M  Palma A  Tedde M  Angioni A  Garau A  Cabras P 《Journal of agricultural and food chemistry》2007,55(3):825-831

Mature apricots (Prunus armeniaca), nectarines [Prunus persica var. nectarine (Ait.)], and peaches [P. persica (L.) Batsch.] were subjected to a 2 min dip treatment with warm water at 48 degrees C or with fludioxonil (FLU) at 100 mg L-1 and 20 degrees C or at 25 mg L-1 FLU and 48 degrees C and then stored at 5 degrees C and 90-95% relative humidity (RH) for 1 week plus 1 additional week at 18 degrees C and approximately 80% RH. Fruit residue uptake was determined as a function of fungicide concentration, dip temperature, treatment time (only on nectarines), and fruit storage conditions. FLU residue level was closely related to fungicide concentration and treatment temperatures and was dependent on fruit species. FLU residues showed great persistence over both storage and shelf life. Fruit dipping in water at 48 degrees C effectively reduced decay development in cvs. 'May Grand' nectarines and 'Pelese' apricots but was ineffective in cvs. 'Red Top' and 'Sun Crest' nectarines during 7 days of storage compared with nontreated fruit. Decay rates in cvs. 'Glo Haven' peaches and 'Fracasso' apricots were very low in fruit dipped in water at both 20 and 48 degrees C. Fungicide treatments at 20 and 48 degrees C resulted in the total or almost total suppression of decay in all cultivars. During shelf life, fruit became very prone to decay, averaging 25.7-100% depending on the cultivar. Fruit dipping in hot water effectively reduced decay in 'Pelese' and 'Fracasso' apricots, 'Sun Crest' peaches, and 'May Grand' nectarines as compared to control, but was ineffective in 'Glo Haven' and 'Red Top' peaches. Fungicide treatments at 20 degrees C were more effective than hot water in most cultivars. The combination of FLU with water at 48 degrees C further improved the fungicide performance. Indeed, reduced levels (a fourth) of active ingredient were required to achieve a control of decay comparable to that for treatment at 20 degrees C. Residue levels in fruit after treatment with 100 mg L-1 FLU at 20 degrees C or with 25 mg L-1 FLU at 48 degrees C averaged approximately 0.6-2 mg kg-1, which were notably lower than the maximum residue limit (5 mg kg-1) allowed in the United States for stone fruit.  相似文献   

Detection of bovine leukemia virus in blood and milk by nested and real-time polymerase chain reactions.   总被引：2，自引：0，他引：2  

Christopher J Kuckleburg  Christopher C Chase  Eric A Nelson  Salvatore A E Marras  Matthew A Dammen  Jane Christopher-Hennings 《Journal of veterinary diagnostic investigation》2003,15(1):72-76

Concerns about retroviruses in livestock and products derived from them have necessitated the development of tests to detect the bovine leukemia virus (BLV) in blood and milk from cattle. Dairy cattle (n = 101) from 5 different geographical areas were used for this study. A nested polymerase chain reaction (PCR) identified 98% of BLV seropositive cattle (n = 80) from blood and 65% from milk, whereas real-time PCR detected 94% of BLV seropositive cattle from blood and 59% from milk. Bovine leukemia virus was also detected by PCR in approximately 10% of seronegative cattle (n = 21), most likely because of early detection before seroconversion.  相似文献   

Participatory tomato breeding for organic conditions in Italy     

G. Campanelli  N. Acciarri  B. Campion  S. Delvecchio  F. Leteo  F. Fusari  P. Angelini  Salvatore Ceccarelli 《Euphytica》2015,204(1):179-197

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Seed germination and conservation of endangered species from the Italian Alps: Physoplexis comosa and Primula glaucescens     

Bruno Cerabolini  Rossella De Andreis  Roberta M. Ceriani  Simon Pierce  Barbara Raimondi 《Biological conservation》2004,117(3):351-356

Physoplexis comosa and Primula glaucescens, endemic to calcareous grasslands of the Lombardy Prealps, northern Italy, are protected under Annex IV of European Community Directive 92/43/EEC. Seed germination dynamics of these species were studied with the aim of producing appropriate germination protocols for use in ex situ conservation. Seeds were collected from Monte Barro (922 m a.s.l.) and for Primula glaucescens from three additional sites in a more central part of its range (1217-2521 m a.s.l.). Physoplexis comosa germinated only in sterile in vitro conditions, in which germination percentages were extremely low without a supply of gibberellic acid (GA3; 1.7%); the optimum GA3 concentration was 100 mg l⁻¹ (> 90% germination), with lower germination at higher doses of 250-500 mg l⁻¹. Primula glaucescens germinated in both sterile and non-sterile conditions and, although total germination was not affected by GA3 concentration, doses of 10-500 mg l⁻¹ acted equally to prompt earlier and more rapid germination. Primula glaucescens from Monte Barro was characterised by slower and poorer germination than from central sites.  相似文献   

Collection of plant genetic resources in Italy 1989     

Karl Hammer  Gaetano Laghetti  Salvatore Cifarelli  Pietro Perrino 《Genetic Resources and Crop Evolution》1990,38(3):311-323

Summary Exploration and collection of plant genetic resources were continued in central Italy 1989—regions Abruzzo, Umbria, Marche, Toscana.Triticum dicoccon was used as an indicator for traditional agriculture. 169 accessions were collected, mainly of cereals, vegetables and grain legumes. Worth mentioning are land-races ofTriticum aestivum, T. durum, Secale cereale, Pisum sativum, Lactuca sativa andCucurbita maxima. Brassica oleracea var.palmifolia, the palm-leaved kale, is an important vegetable in the Toscana. Several other rare crops could be also collected.

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Sammlung pflanzlicher genetischer Ressourcen in Italien 1989

Zusammenfassung Die Exploration und Sammlung pflanzlicher genetischer Ressourcen wurden 1989 in Mittelitalien fortgesetzt — Regionen Abruzzo, Umbria, Marche, Toscana.Triticum dicoccon wurde als Indikator für traditionelle Landwirtschaft verwendet. 169 Proben wurden gesammelt, hauptsächlich von Getreiden, Gemüsen und Körnerleguminosen. Erwähnenswert sind Landsorten vonTriticum aestivum, T. durum, Secale cereale, Pisum sativum undCucurbita maxima. Brassica oleracea var.palmifolia, der Palmkohl, ist ein wichtiges Gemüse in der Toscana. Verschiedene andere seltene Fruchtarten konnten ebenfalls gesammelt werden.

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1989

1989 , , , .Triticum dicoccon . 169 , , . Triticum aestivum, T. durum, Secale cereale, Pisum sativum Cucurbita maxima. Brassica oleracea var.palmifolia, , . .

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New insights into the photoreactivity of the organophosphorus pesticide fenthion: a sigma aryl cation as a key intermediate in the photodecomposition     

Torrisi S  Sortino S 《Journal of agricultural and food chemistry》2004,52(19):5943-5949

The primary photodegradation processes of fenthion (FN), one of the most photosensitive pesticides used in agriculture, have been investigated by combining laser flash photolysis and steady-state measurements. The triplet state of FN is produced quite efficiently (Phi(isc) approximately 0.3). However, this excited state does not seem to trigger the primary photodecomposition pathways of the pesticide. It is demonstrated that FN undergoes photoheterolysis via the excited singlet state and gives the corresponding singlet "sigma aryl cation". Chemical evidence for the generation of this transient species is given by trapping with typical "sigma nucleophiles" such as chloride and methanol. This photodegradation mechanism is, in part, quite different with respect to what is known to date and may account for the formation of the O,O-dimethyl S-[3-methyl-4-(methylthio)phenyl] phosphorothioate discovered as a novel photodegradation product of FN.  相似文献   

Antioxidant characterization of some Sicilian edible wild greens     

Salvatore S  Pellegrini N  Brenna OV  Del Rio D  Frasca G  Brighenti F  Tumino R 《Journal of agricultural and food chemistry》2005,53(24):9465-9471

Epidemiological studies have demonstrated that many antioxidants and the total antioxidant capacity (TAC) of the diet may protect against cancers and cardiovascular disease. Common fruits and vegetables are good sources of antioxidants, although in some Mediterranean areas traditional wild greens are responsible for a significant percentage of total dietary antioxidant intake. In the European Prospective Investigation into Cancer and Nutrition cohort of Ragusa (Sicily), a high number of subjects were found to frequently eat wild greens, including Sinapis incana and Sinapis nigra, Diplotaxis erucoides, Cichorium intybus, Asparagus acutifolius, and Borrago officinalis. On the basis of these observations, detailed characterization of single antioxidant components (i.e., polyphenols, carotenoids, chlorophylls, and ascorbic acid) and the TAC of these edible wild traditional plants was performed. The wild plants examined were found to be very rich in antioxidants, such as flavonoids and carotenoids, with high TAC values, suggesting that the importance of these vegetables, not only in the traditional but even in the contemporary diet, needs to be emphasized.  相似文献   

Silver-catalyzed C-C bond formation between methane and ethyl diazoacetate in supercritical CO₂     

Caballero A  Despagnet-Ayoub E  Díaz-Requejo MM  Díaz-Rodríguez A  González-Núñez ME  Mello R  Muñoz BK  Ojo WS  Asensio G  Etienne M  Pérez PJ 《Science (New York, N.Y.)》2011,332(6031):835-838

Even in the context of hydrocarbons' general resistance to selective functionalization, methane's volatility and strong bonds pose a particular challenge. We report here that silver complexes bearing perfluorinated indazolylborate ligands catalyze the reaction of methane (CH(4)) with ethyl diazoacetate (N(2)CHCO(2)Et) to yield ethyl propionate (CH(3)CH(2)CO(2)Et). The use of supercritical carbon dioxide (scCO(2)) as the solvent is key to the reaction's success. Although the catalyst is only sparingly soluble in CH(4)/CO(2) mixtures, optimized conditions presently result in a 19% yield of ethyl propionate (based on starting quantity of the diazoester) at 40°C over 14 hours.  相似文献   

Detection of Ramularia collo-cygni DMI- and SDHI-resistant field populations in Austria and the effect of fungicides on the population and genetic diversity     

Assinger  Thomas  Fountaine  James  Torriani  Stefano  Accardo  Salvatore  Bernhard-Frey  Regula  Gottula  John  Steinkellner  Siegrid 《European journal of plant pathology / European Foundation for Plant Pathology》2022,162(3):575-594

European Journal of Plant Pathology - Winter barley (Hordeum vulgare L.) is the third most cultivated crop after corn and wheat in Austria but one of the most challenging for disease control. The...  相似文献