猪胆囊中沙门菌L型的分离培养与invA基因检测     

佘晓玲  王涛  丁文静  吴文娟  潘耀振  汤可立  王丹霓  王和 《动物医学进展》2015,(3):25-28

为了解猪胆囊中沙门菌L型携带情况,在贵阳市屠宰场采集970例健康生猪的胆囊组织与胆汁标本,用常规细菌学方法和非高渗分离培养法分离沙门菌及其细菌L型,用PCR和核酸序列分析方法对稳定L型纯培养物进行沙门菌的invA基因检测。结果显示,970例生猪胆囊标本未检出沙门菌细菌型,细菌L型检出率为8.25%;80例细菌L型分离物中有50例invA检测阳性,占5.15%;占细菌L型阳性分离物62.50%。研究结果为生猪胆囊沙门菌L型感染的流行病学及其检查提供了依据。  相似文献   

Characterization and Gene Mapping of a Lesion Mimic Mutant g303 in Rice     

LIU Lin  #  ZHANG Ying xin  #  LI Zhi  LIU Qun en  YU Ning  SUN Bin  YANG Zheng fu  ZHOU Quan  CHENG Shi hua  CAO Li yong 《中国水稻科学》2014,28(5):465

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三江平原主栽水稻品种生育进程对温度的响应     

马瑞  黄成亮  刘猷红  孟英  董文军  付久才  刘伟  王庆胜 《黑龙江农业科学》2020,(11):24-28

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蚌埠市城市土地生态安全评价及障碍因子诊断     

陆雅雯  何如海  李青松  周颖 《黑龙江八一农垦大学学报》2020,32(3):85-92

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齐齐哈尔市鲜食玉米品种比较试验     

李青超  王立达  赵秀梅  刘洋  韩业辉  王俊强  杨莹 《黑龙江农业科学》2020,(7):11-14

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Two Complete Mitochondrial Genomes From Leuctridae (Plecoptera: Nemouroidea): Implications for the Phylogenetic Relationships Among Stoneflies     

Jinjun Cao  Ying Wang  Xuan Guo  Guoquan Wang  Weihai Li  Dvid Murnyi 《Journal of insect science (Online)》2021,21(1)

The family-level relationships within Plecoptera have been a focused area of research for a long time. Its higher classification remains unstable, and the phylogenetic relationships within Plecoptera should be re-examined. Here, we sequenced and analyzed two complete mitochondrial genomes (mitogenomes) of Paraleuctra cercia and Perlomyia isobeae of the family Leuctridae. We reconstructed the phylogeny of Plecoptera based on 13 protein-coding genes (PCGs) from published stoneflies. Our results showed that the Bayesian inference and maximum-likelihood tree had similar topological structures except for the positions of two families, Peltoperlidae and Scopuridae. The Plecoptera is divided into two clades, the suborder Antarctoperlaria and the suborder Arctoperlaria. The two suborders subsequently formed two groups, Eusthenioidea and Gripopterygoidea, and Euholognatha and Systellognatha, which is consistent with the results of morphological studies. In addition, the Leuctridae is the earliest branch within the superfamily Nemouroidea. But the monophyly of Perloidea and Pteronarcyoidea are still not well supported.  相似文献   

光对园艺植物花青素生物合成的调控作用   总被引：2，自引：0，他引：2  

王峰  王秀杰  赵胜男  闫家榕  卜鑫  张颖  刘玉凤  许涛  齐明芳  齐红岩  李天来 《中国农业科学》2020,53(23):4904-4917

花青素是植物中一类重要的类黄酮化合物,在植物花朵、果实等器官色泽形成和抗氧化过程中起着重要作用。植物组织中花青素的形成依赖于光信号,但是光信号对花青素生物合成的调控机制及信号网络很大程度上还不清晰。本文简述了花青素生物合成及运转过程的研究进展,简要归纳了MYB、bHLH、WDR三类主要因子对花青素合成的转录调控作用,重点阐释光信号（光强、光质、光照时长）对植物花青素合成的调控作用。研究表明,光环境（光强、光质、光照时长）主要通过不同的光受体（UVR8、CRYs、PHOTs、PHYs）影响光信号通路重要因子COP1的泛素化能力和HY5的稳定性,以及其他光信号转录因子如光敏色素互作因子PIFs的稳定性,进而调控花青素的生物合成过程。这些光信号因子一方面直接结合到调控花青素合成的MYB、bHLH、WDR三大类转录因子上,转录激活或抑制它们的表达进而调控花青素的合成;另一方面,这些光信号因子通过与MYB、bHLH、WDR三大类转录因子蛋白互作,影响它们形成的MBW复合体稳定性,进而调控花青素的合成。此外,这些光信号因子还可以通过不依赖于MBW复合体的通路调控花青素的合成,如HY5通过调控miR858影响花青素的生物合成;另外,一些未知的光响应因子可能以不依赖MBW通路的方式直接或间接地调控花青素合成基因和液泡膜上的运转蛋白,改变液泡酸化,调节花青素的合成。同时,光信号会影响光合电子传递,光合电子传递链中的一些因子也会通过依赖和不依赖MBW的途径影响植物花青素的合成。这些途径如何协调以及哪些信号因子优先受光环境（光强、光质、光照时间）调控?本文为深入研究光信号对花青素生物合成的调控机理提供参考,以探索光调控花青素积累的有效途径及靶标分子,为利用基因工程、代谢工程和光环境调控手段改良园艺植物花青素积累提供理论基础。  相似文献   

五指山市乔木林动态变化特征分析     

刘立武  佘济云  程玉娜 《中南林业科技大学学报(自然科学版)》2014,(4):1-5

为了了解五指山市乔木林的空间分布特征,掌握主要林分因子的发展趋势,结合海南省五指山市四期森林资源连续清查数据,运用ArcGIS10.0、SPSS软件,对五指山市连续15 a乔木林地的变化进行分析。结果表明:(1)乔木林为五指山市主要的用地类型,且数目逐年增加;(2)乔木林分主要分布在海拔在500~1 000 m、坡度在15°~35°之间向阳的空间范围内;(3)平均年龄为28.54,平均树高为11.41 m,平均胸径为13.46 m,平均每亩蓄积为8.86 m3,平均郁闭度为0.609。从龄组结构来看,大多属于中龄林与幼龄林,处于正演替阶段。  相似文献   

Variations of SOC and MBC observed in an incubated brown loam soil managed under different tillage systems for 12 years     

Xiaosha Li  Huifang Han  Tangyuan Ning  Ying Shen  Rattal Lal 《Soil Use and Management》2019,35(4):585-594

To assess changes in organic carbon pools, an incubation experiment was conducted under different temperatures and field moisture capacity (FMC) on a brown loam soil from three tillage practices used for 12 years: no‐till (NT), subsoiling (ST) and conventional tillage (CT). Total microbial respiration was measured for incubated soil with and without the input of straw. Results indicated that soil organic carbon (SOC) and microbial biomass carbon (MBC) under ST, NT and CT was higher in soil with straw input than that without, while the microbial quotient (MQ or MBC: SOC) and metabolic quotient (qCO₂) content under CT followed the opposite trend. Lower temperature, lower moisture and with straw input contributed to the increases in SOC concentration, especially under NT and ST systems. The SOC concentrations under ST, with temperatures of 30 and 35°C after incubation at 55% FMC, were greater than those under CT by 28.4% and 30.6%, respectively. The increase in MBC was highest at 35°C for 55%, 65% and 75% FMC; in soil under ST, MBC was greater than that under CT by 199.3%, 50.7% and 23.8%, respectively. At 30°C, the lower qCO₂ was obtained in soil incubated under NT and ST. The highest MQ among three tillage practices was measured under ST at 55% FMC, NT at 65% FMC and CT at 75% FMC with straw input. These data indicate the benefits of enhancing the MQ; the low FMC was beneficial to ST treatment. Under higher temperature and drought stress conditions, the adaptive capacity of ST and NT is better than that of CT.  相似文献   

甜樱桃新品种晓文1号及栽培技术     

李莹  王利荣 《山西果树》2015,(3):18-20

晓文1号甜樱桃新品种具有品质优、抗性强、丰产、稳产等特性,其对园地、砧木、授粉树、栽植密度及整形修剪等都有具体要求,生产中应把握好各项技术,以保证栽培的成功。  相似文献