Populus caspica Bornm.(section Leuce and subsection Albida), one of the most endangered endemic tree species in the Hyrcanian Forest in Iran, has numerous morphological characteristics that are closely similar to Populus alba; to clarify their taxonomic relatedness and genetic differentiation and thus inform conservation strategies, we used the noncoding regions of chloroplast DNA(cpDNA; trnL-F and trnH-psbA) and the internal transcribed spacer(ITS). Leaf samples were collected from six populations across northern Iran. cpDNA and ITS fragments were amplified by universal primers using the PCR technique and directed sequencing. The results showed that P. caspica is genetically differentiated from P.alba, and two ITS variants were detected within some P.caspica individuals. Conflicts between topologies from ITS and plastid genomes were observed. High differentiation of P. caspica from the other Populus species shown in this study confirmed the diverging taxonomic status of this endangered species. We recommend in situ conservation measures(e.g., protected areas) for at least several populations of this species, especially in the plain regions of the Hyrcanian forest. 相似文献
Inhibition of nitrification as a mitigation tool to abate nitrogen (N) losses and improve N use efficiency (NUE) is a promising technology. Nitrification inhibitor (dicyandiamide, DCD) was evaluated in two consecutive wheat-maize rotations (2015–2017), with two different N fertilizer levels applied in wheat (160, 220 kg N ha?1) and maize (180, 280 kg N ha?1). More NH4+-N contents (101% and 102% in wheat and 74% and 73% in maize) and less NO3–-N contents (37% and 43% in wheat and 46% and 57% in maize) were observed at both N levels treated with DCD compared to without DCD. Higher pH, lower EC and reduced NO3–-N accumulation were the other benefits of DCD. The NO3–-N accumulation within the 0–200 cm soil profile was significantly less at both N levels with DCD (66 mg kg?1 and 121 mg kg?1) compared to without DCD (96 mg kg?1 and 169 mg kg?1). Application of DCD also improved the growth and yield in both crops. Increase in NUE from 38% to 49% in wheat and 27% to 33% in maize with DCD at higher N level was also observed. Overall, the effectiveness of DCD in retarding the nitrification process was higher in wheat than maize. 相似文献
The anticonvulsant activities of Crocus sativus stigma constituents, safranal and crocin, were evaluated in mice using pentylenetetrazole (PTZ)-induced convulsions in mice. Safranal (0.15 and 0.35 ml/kg, i.p.) reduced the seizure duration, delayed the onset of tonic convulsions and protected mice from death. Crocin (200 mg/kg, i.p.) did not show anticonvulsant activity. 相似文献
The aims of the present study were to investigate the prevalence of some virulence genes and also determine the antimicrobial resistance pattern of E. coli isolated from bovine with subclinical mastitis. The milk of 502 cows was collected from 8 dairy herds in the southwest of Iran. Conventional biochemical tests were used for identification of E. coli at the species level. Antimicrobial susceptibility patterns of E. coli isolates were determined by disc agar diffusion method and polymerase chain reaction (PCR) was used for detection of seven virulence genes including f17A, afaE-8, afaD-8, eaeA, cnf1, cnf2, and iucD. Seventy (13.94%) isolates of E. coli were identified in 502 milk samples. The highest rate of resistance was observed against tetracycline (18.6%), while none of the isolates were resistant to streptomycin. Eight (11.5%) out of 70 E. coli isolates carried at least one of the virulence genes. The afaD-8 was the most prevalent gene detected in 5 (7.1%) isolates. The afaE-8, iucD, and eaeA were detected in 3, 3, and 2 isolates respectively. Low prevalence of virulence factors may be indicating that most of the E. coli isolates originated from the commensal flora of cows and enter to the udders via environment contamination with feces.
The genetic basis of salt tolerance was examined in selected salt tolerant and sensitive material from a sample of accessions
previously assessed for variability in salinity tolerance. The North Carolina Model 2 Design and analysis was followed, tolerance
being assessed in 10-day-old seedlings grown in salinized solution culture at control (0 mM), 60 mM and 80 mM NaCl concentrations).
Salinity tolerance was shown to be under the control of genes with additive and non-additive effects, with broad and narrow
sense heritability estimates being approximately 0.7 and 0.4 over all treatments.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
Genetic Resources and Crop Evolution - The Saccharum species members are extremely complex allopolyploids. In this study, sixty Saccharum spp. hybrids were analyzed using simple sequence repeat... 相似文献
The deficiency of micronutrients, particularly zinc (Zn) and boron (B) has affected vegetative growth, yield and fruit quality of sweet orange in many citrus growing areas of Pakistan. The present study was conducted in 2015 and 2016 to determine the impact of Zn and B supplementation on growth and quality of sweet orange cv. ‘Blood red’. Pre-harvest foliar application of variant Zn and B levels was done at different phenological stages of fruit growth and development, i.e., full bloom, fruit set and premature stage. Results have concluded that pre-harvest foliar application of Zn and B augmented phosphorous, Zn, B, potassium, iron, and manganese contents in leaves, particularly Zn and B content from deficient to optimal level. Moreover, the combined application of Zn and B has also significantly enhanced vegetative characters such as tree height and spread along with reproductive characters, i.e., incremented numbers of fruit, fruit weight, total soluble solids, titratable acidity and ascorbic acid contents. However, among stage comparison, outcomes depicted that foliar application of Zn and B at full bloom?+?fruit set?+?premature stage resulted in maximum production with high quality fruits.
Background: Defects in endoplasmic reticulum homeostasis are common occurrences in different diseases, such as diabetes, in which the function of endoplasmic reticulum is disrupted. It is now well established that ion channels of endoplasmic reticulum membrane have a critical role in endoplasmic reticulum luminal homeostasis. Our previous studies showed the presence of an ATP-sensitive cationic channel in endoplasmic reticulum. Therefore, in this study, we examined and compared the activities of this channel in control and diabetic rats using single-channel recording techniques. Method: Male Wistar rats were made diabetic for 2 weeks with a single dose injection of streptozotocin (45 mg/kg). Ion channel incorporation of rough endoplasmic reticulum of diabetic hepatocytes into the bilayer lipid membrane allowed the characterization of K+ channel. Results: Ion channel incorporation of rough endoplasmic reticulum vesicles into the bilayer lipid revealed that the channel current-voltage (I-V) relation with a mean slope conductance of 520 ± 19 pS was unaffected in diabetes. Interestingly, the channel Po-voltage relation was significantly lower in diabetic rats at voltages above +30 mV. Conclusion: We concluded that the endoplasmic reticulum cationic channel is involved in diabetes. Also, this finding could be considered as a goal for further therapeutic plans. Key Words: Endoplasmic reticulum, Diabetes, Ion channels, Bilayer lipid membrane, Liver相似文献
Topography is the most factor that has the greatest impact of all factor that affect the distribution.To study the diversity of trees and shrub species in the Perc forest situated in Khorramabad,Lorestan,140 circular plots of 1200 m~2 in a grid of 300 m 9 250 m were surveyed,using a systematic random sampling method.In each plot,the Margalef richness index,Shannon–Wiener diversity index,Hill's N_1 and Simpson indices and the evenness index of Simpson and Smith-Wilson were calculated and ordered on the basis of different classes of elevation,exposition and slope.The results indicated that slope did not have any significant effect on the indices.Exposition and elevation classes significant impacted the richness and diversity indices,but did not influence evenness.In general,the highest plant diversity was observed for slopes less than 15 %,northern aspects,without geographical direction,and elevations of 2100–2200 m.This information can be very useful in achieving the goals for sustainable management of forests.In addition to greater protection for regions with high diversity and reforestation(compatible species) in degraded area,we can help increase diversity in forests. 相似文献
