Effect of dietary n-3 polyunsaturated fatty acid supplementation on the expression of genes involved in progesterone biosynthesis in the corpus luteum of goat (Capra hircus)     

Ravjibhai Karshanbhai Chaudhari  Ajit Singh Mahla  Sanjay Kumar Singh  Abhijit M. Pawde  Surendra Kumar Badasara  Harendra Kumar  Manas Kumar Patra  Narayanan Krishnaswamy 《Reproduction in domestic animals》2020,55(9):1263-1266

  相似文献   

Histamine receptors mediate contraction of reticular groove smooth muscle of adult goats     

V. A. Aneesha  Renjith P. Gopi  Sanjay Kumawat  V. S. Susanth  S. Vineetha  Dinesh Kumar 《Anatomia, histologia, embryologia》2020,49(4):440-450

The present study was conducted to evaluate the effect of histamine and to characterise its receptor subtypes in reticular groove (RG) smooth muscle of adult goats. The studies were done using floor and lip regions of RG. We used tension experiments on smooth muscle of RG isolated from adult goat for functional characterisation of H₁ and H₂ receptors. Western blotting and immunohistochemistry experiments were conducted for molecular characterisation of these receptors. Histamine evoked concentration-dependent contraction of isolated RG circular and longitudinal smooth muscle preparation. Pyrilamine antagonised the action of histamine. Histamine did not induce any relaxant effect on RG preparations. Additionally, cimetidine did not produce any significant effect on histamine-induced response. Non-selective histaminic receptor antagonist cyproheptadine attenuated the contraction response to histamine in the smooth muscle. Molecular characterisation and localisation of H₁ and H₂ receptor proteins confirmed the presence of these receptors in RG. It is most likely that histamine-induced contractile effect in RG smooth muscle of goats is mediated by H₁ histaminic receptors.  相似文献   

Synergistic effect of inoculating plant growth-promoting Pseudomonas spp. and Rhizobium leguminosarum-FB1 on growth and nutrient uptake of rajmash (Phaseolus vulgaris L.)     

Pankaj Kumar Mishra  Shekhar Chandra Bisht  Krishnan Jeevanandan  Sanjay Kumar  Jaideep Kumar Bisht  Jagdish Chandra Bhatt 《Archives of Agronomy and Soil Science》2013,59(6):799-815

Plant growth-promoting bacteria (PGPB) Pseudomonas lurida-NPRp15 and Pseudomonas putida-PGRs4 possessing multiple plant growth-promoting traits were isolated from rhizoplane of pea and rhizosphere of garlic, respectively. The effects of individuals and combinations of Pseudomonas spp. with effective root nodulating symbiotic nitrogen fixing Rhizobium leguminosarum-FB1 on plant growth, nutrient uptake and yield of the rajmash plant were studied under greenhouse conditions. Bacterial inoculation resulted in significantly higher values for plant dry biomass, N, P, K, Zn and Fe contents as compared to the uninoculated control. Furthermore, dual inoculation of P. lurida-NPRp15 with R. leguminosarum-FB1 significantly increased root and shoot dry weight, nodulation, nutrient uptake, pod yield, and nutrient content of pods of rajmash VL63 compared to controls, single and triple inoculation. The results of the study indicate the potential of harnessing the benefit of plant growth-promoting and nitrogen-fixing microorganisms to improve the growth and yield of rajmash.  相似文献   

Prospective use of bacteriocinogenic Pediococcus pentosaceus as direct-fed microbial having methane reducing potential     

Sanjay Kumar  Sumit S Dagar  Seyed H Ebrahimi  Ravinder K Malik  Ramesh C Upadhyay  Anil K Puniya 《中国农业科学(英文版)》2015,(3):561-566

Direct-fed microbials(DFM), generally regarded as safe status, are successfully used in improving rumen ecology, gastro-intestinal health, feed efficiency, milk production and growth rate in ruminants. On the other hand, methanogenesis in rumen, which accounts for a significant loss of ruminant energy and increased greenhouse gas in environment, is of great concern, therefore, use of DFM for improving productivity without compromising the animal health and ecological sustainability is encouraged. The present study was conducted to investigate the methane reducing potential of bacteriocinogenic strain Pediococcus pentosaceus-34. Since, the culture showed no hemolysis on blood agar and DNase activity, hence, it was considered to be avirulent in nature, a prerequisite for any DFM. The culture also showed tolerance to pH 5.0 for 24 h with 0.5% organic acid mixture, whereas when given a shock for 2 h at different p H and organic acids concentrations, it showed growth at pH 3.0 and 4.0 with 0.1 and 1.0% organic acids, respectively, as having good animal probiotics attributes. The total gas production was significantly(P<0.05) higher in live pedicoccal culture(LPC) and dead pedicoccal culture(DPC) both with wheat straw, when compared to the control. In sugarcane bagasse, gas production was significantly lower(P<0.05) with LPC compared to the control and DPC both. Methane was reduced by the inclusion of LPC in sugarcane bagasse(0.07 mL CH4 mg–1 dry matter digestibility) with no effect on other rumen fermentation parameters. However, with wheat straw and LPC total gas, in vitro dry matter digestibility, total volatile fatty acids increased significantly but no reduction in methane production was observed in comparison to the control. Therefore, further research is warranted in this direction, if the bacteriocinogenic strains can be used as DFM for ruminants to improve the ruminant productivity.  相似文献   

Genetic difference in adventitious rhizogenesis in <Emphasis Type="Italic">Albizia procera</Emphasis> Benth. with IBA treatment     

Shamim Akhtar Ansari  Sanjay Singh 《Journal of Forest Research》2008,13(1):79-82

Variable adventitious rhizogenesis of juvenile shoot cuttings was observed in seven half-sib families (TP-1, BAN-1, AN-3, JR-3, SL-16, UM-3, and UM-5) of Albizia procera Benth. Treatment with IBA invariably promoted sprouting by 8%, adventitious rooting by 218%, root number by 869%, and root length by 36.4% compared with the control. Interaction between families and IBA treatment significantly enhanced sprouting and root length in BAN-1, JR-3, UM-3, and UM-5, and root number in all families, but had a significant suppressive effect on sprouting in AN-3. Treatment with IBA increased family heritability (h _f ²) 1.38-fold for sprouting, 2.86-fold for adventitious rooting, and 10.86-fold for root number, but reduced family heritability by a factor of 8.25 for root length. Thus, direct genetic effects seem to have strongly affected sprouting and adventitious rooting, with IBA playing an auxiliary role, but had little effect on root number and length, for which IBA had a dominant regulatory role.  相似文献   

Tea (Camellia sinensis) clones with shorter periods of winter dormancy exhibit lower accumulation of reactive oxygen species   总被引：1，自引：0，他引：1  

Vyas D  Kumar S  Ahuja PS 《Tree physiology》2007,27(9):1253-1259

Tea (Camellia sinensis (L.) O. Kuntze) is a perennial crop grown throughout the world. During winter, tea undergoes a dormancy period when growth of apical buds almost ceases, severely reducing the commercial yield of tea. Low temperatures prevail during the period of winter dormancy, which alone or in combination with high solar irradiance have the potential to induce oxidative stress in plants. We studied six tea clones under field conditions to test whether a relationship exists between oxidative stress and winter dormancy. Data on the behavior of the enzymatic antioxidative system was collected for all clones during different phases of winter dormancy. There was a strong positive correlation among clones between accumulation of reactive oxygen species (ROS) and the length of the dormancy period. Clones having shorter dormancy periods exhibited higher induction of antioxidative enzymes. Results suggest that efficient scavenging of ROS is a desirable feature in tea because it leads to lower accumulations of ROS during winter months and is associated with reduced winter dormancy.  相似文献   

Genetic divergence studies on reproduction and production traits among Gir crosses     

Mahendra Gorakh Mote  Sangita Uddhav Bhoite  Yogesh Chandrakant Bangar  Sanjay Mandakmale 《Tropical animal health and production》2018,50(8):1881-1885

The study was undertaken to estimate the genetic divergence among FG, IFG, FJG, IFJG, and R crosses of Gir cow on the basis of age at first conception, age at first calving, and lactation milk yield per day of lactation length using Mahalanobis D² statistics. The genetic groups’ influence was significant (P?<?0.01) for all traits separately and simultaneously (V test) based on three traits. The differences in the D² values among all the genetic groups’ combinations were significant except IFG with R genetic group combination. The total D² values for age at first conception (AFCon), age at first calving (AFC), and lactation milk yield per day of lactation length (LMY/LL) were 18.85, 0.06, and 9.01 respectively. The percent contribution of AFCon to the total D² value was maximum as 67.51 followed by LMY/LL as 32.27 and lowest of AFC as 0.22. Among the clusters formed on the basis of D² values, IFG, IFJG, and R formed one cluster, whereas, FG and FJG formed second cluster. The magnitude of inter-cluster distance was greater than intra-cluster distance.  相似文献   

Repurposing Ionophores as novel antimicrobial agents for the treatment of bovine mastitis caused by Gram‐positive pathogens          下载免费PDF全文

Elizabeth E. Hickey  Hui San Wong  Manouchehr Khazandi  Abiodun D. Ogunniyi  Kiro R. Petrovski  Sanjay Garg  Stephen W. Page  Ryan O'Handley  Darren J. Trott 《Journal of veterinary pharmacology and therapeutics》2018,41(5):746-754

Increasing reports of multidrug‐resistant bacterial infections in animals has created a need for novel antimicrobial agents that do not promote cross‐resistance to critically important antimicrobial classes used in human medicine. In response to the recent emergence of antimicrobial resistance in several bovine mastitis pathogens, in vitro antimicrobial susceptibility was determined for four polyether ionophores (lasalocid, monensin, narasin and salinomycin) against Staphylococcus spp. and Streptococcus spp. isolated from clinical cases. In addition, erythrocyte haemolysis and WST ‐1 cell proliferation assays were used to assess in vitro mammalian cell cytotoxicity and biofilm susceptibility testing was performed using the minimum biofilm eradication concentration (MBEC ?) biofilm assay. Lasalocid, monensin, narasin and salinomycin exhibited bacteriostatic antimicrobial activity against all pathogens tested, including methicillin‐resistant staphylococci, with MIC ₉₀ values <16 μg/ml. Narasin and monensin displayed the least toxicity against mammalian cell lines and all compounds significantly reduced viable cell numbers in a Staphylococcus aureus biofilm. Based on in vitro characterization, all four ionophores offer potentially novel treatments against bovine mastitis but in vivo studies will be essential to determine whether acceptable safety and efficacy is present following intramammary administration.  相似文献   

Stockplant Management for Optimized Rhizogenesis in Tectona grandis Stem Cuttings     

Sanjay Singh  A. S. Bhandari  S. A. Ansari 《New Forests》2006,31(1):91-96

A 5-year stand of teak (Tectona grandis L. f.) was coppiced in 1999 and converted into a vegetative multiplication garden. Subsequently, three harvesting regimes for the collection of single node stem cuttings were imposed: (1) once – in March (H₁), (2) twice – in March and September (H₂) and (3) three times in March, July and November (H₃). Cuttings were treated basally with either:- T₀ – control (6 h in water), T₁ – half the recommended dose of a mixture of IBA and thiamine (500 ppm IBA + 400 ppm thiamine) or T₂ – the full dose of the same mixture (1000 ppm IBA + 800 ppm thiamine). Cuttings receiving IBA + thiamine rooted significantly better than untreated cuttings, but even the best treatment only resulted in 38.3 ± 3.8% rooting. This treatment produced the greatest number of roots (5.2–12.1). The full dose treatment appears to have been supra-optimal. Rooting ability was also affected by the frequency of stockplant pruning, with cuttings from stockplants pruned twice per year having the greatest rooting percentage (27.8 ± 3.8%) and the most roots (9.2 ± 4.8). This bi-annual pruning (H₂) resulted in the greatest number of rooted propagules (2.6 and 4.2 times more than H₁ and H₃, respectively). There was a significant interaction between Treatment × Pruning frequency. Bi-annual hedging of teak stockplants is recommended for practical purposes, although further work is required to achieve commercially acceptable levels of rooting from coppiced tree stumps.  相似文献   

The history of avian influenza     

Lupiani B  Reddy SM 《Comparative immunology, microbiology and infectious diseases》2009,32(4):311-323

  相似文献