Clinicopathologic findings of naturally occurring Rabbit Hemorrhagic Disease Virus 2 infection in pet rabbits     

Cristina Bonvehí  María Ardiaca  Andrs Montesinos  Carles Juan‐Salls  Alicia Gmez  Beatriz Teso  Sergio Barbero  Sara Ekei Ferrera 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2019,48(1):89-95

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Enrichment of Adult Artemia Biomass and Squid Mantle Muscle,Dosidicus gigas,with Different Ascorbic Acid (L‐Ascorbyl‐2‐Monophosphate‐Na/Ca) Concentrations          下载免费PDF全文

Miguel E. Marentes‐Montes  Jesús T. Ponce Palafox  Sergio Castillo‐Vargasmachuca  Irma E. Martínez‐Rodríguez  Jorge Castro Mejia  Manuel García‐Ulloa  Alireza Seidavi 《Journal of the World Aquaculture Society》2015,46(2):219-227

L‐ascorbyl‐2‐monophosphate‐Na/Ca (AMP‐Na/Ca) was used as a vitamin C source to investigate its ascorbic acid (L‐AA) enrichment and retention in boosted Artemia biomass (AB) and squid mantle muscle (SM). Different doses of AMP‐Na/Ca (500, 1000, and 1500 AMP‐Na/Ca mg/kg) were gradually dissolved into the culture tanks at time 0 (T₀) and at each hour until Hour 6 (T₆). Samples of AB and SM were taken for AMP‐Na/Ca and L‐AA analysis at T₀, T₁, T₂, T₃, T_4, T₅, T₆, T₁₂, and T₂₄. There were no significant differences (P > 0.05) among the AB groups at T₁. The T₆ enrichment analysis for AB resulted in significant differences (P < 0.05) in the AMP‐Na/Ca content for the 1500 mg/kg treatment, in which the initial concentration (0.001 ± 0.002 mg/kg) increased by more than 16‐fold. For all AB enrichment treatments, the AMP‐Na/Ca content demonstrated a decrease (32–11%) for the T₆, T₁₂ and T₂₄ analysis. The T₁ analysis for SM at the higher AMP‐Na/Ca enrichment concentration registered 30 mg/kg of L‐AA and decreased (27.6%) at T₆. This study demonstrated that AB and SM can be boosted with AMP‐Na/Ca.  相似文献   

Transgenic poplar with enhanced growth by introduction of the ugt and acb genes     

Rurick Salyaev  Natalya Rekoslavskaya  Anastasia Chepinoga  Sergio Mapelli  R. Pacovsky 《New Forests》2006,32(2):211-229

The transgenic poplar (Populus tremula L.) was obtained by transfer of the ugt and acb genes via triparental mating, which was employed to deliver large fragments of TDNA as a cluster. Freshly harvested seeds of local poplar were placed on MS agar medium and plantlets were obtained. After 1 year of subcultivation, plantlets were infected with a transconjugant of triparental mating with target ugt and acb genes into axillary buds. The transformed sprouts so obtained were cut and subcultivated on agar medium with an addition of 0.6 mg/l indole-3-butyric acid as an auxin source. The transformed sprouts showed GUS activity and resistance to gentamycin and kanamycin. The integrity of the target ugt and acb genes into poplar genome was demonstrated via PCR and Southern blot hybridisation. The transgenic poplar plants revealed a higher growth energy, corresponding to a higher content of IAA as opposed to control plants. Both transgenic and non-transformed plants were potted into soil for outdoor acclimatisation and subsequently transferred to earth in beds. Growing outside during 3 years, the transgenic poplar demonstrated a higher growth rate with fast bud and branch development.  相似文献   

Extraction and characterization of volatile compounds in Maytenus ilicifolia, using high-pressure CO2     

Mossi AJ  Cansian RL  Carvalho AZ  Dariva C  Oliveira JV  Mazutti M  Filho IN  Echeverrigaray S 《Fitoterapia》2004,75(2):168-178

This work is focused on the chemical characterization of Maytenus ilicifolia extracts obtained from high-pressure CO2 extraction. The experiments were performed in a laboratory-scale unit, where the effect of particle size (35-200 mesh), CO2 mass flow rate (1-3 g/min), temperature (293-323 K), pressure (100-250 bar), and extraction time was investigated in terms of liquid yield and chemical composition of the extracts. Results show that the particle size and CO2 mass flow rate did not affect the liquid yield, whereas the extraction temperature and solvent density exerted a pronounced effect on both liquid yield and chemical distribution of volatile compounds. The extracts were chemically analyzed with regard to phytol, squalene, vitamin E, stigmasterol, friedelan-3-ol, friedelin, dodecanoic acid and geranyl acetate in a GC/MSD.  相似文献   

Combined use of surgery and radiation in the treatment of an intradural myxoid liposarcoma in a dog     

Rodenas S  Valin I  Devauchelle P  Delisle F  Baron M 《Journal of the American Animal Hospital Association》2006,42(5):386-391

An intradural-extramedullary myxoid liposarcoma of the high cervical spine was diagnosed in a 9-year-old, spayed female Cavalier King Charles spaniel that was presented for a 2-month history of cervical pain and tetraparesis. Radiation therapy applied after surgery resulted in complete remission of the neurological deficits. The tumor recurred 18 months after surgical excision. A second surgery and another course of radiotherapy again resulted in complete remission of the clinical signs. The dog was euthanized 11 months after the second surgery because of tumor recurrence.  相似文献   

Evaluation of an agricultural experiment station as a case study site for the establishment of a multi-use urban forest     

Sergio Santiago-Meléndez  Sally González  Ricardo Goenaga 《Urban Forestry & Urban Greening》2012,11(4):406-416

In an attempt to contribute towards a more sustainable development for the island of Puerto Rico, this project focuses on the documentation and evaluation of the Tropical Agriculture Research Station (TARS) near the urban center of the city of Mayagüez, Puerto Rico as a case study site to establish a multi-use and research-oriented urban forest. This location has been used since the beginning of the 20th century as a plant introduction and evaluation site, and as an in situ germplasm collection for numerous plant species. The evaluation methods consisted of identifying unique resources within the study area and stressors which could affect the latter. Unique resources were also identified on a Preliminary Resource Inventory Map (PRIM) using GPS technology. Obtained results and observations indicate that the study area represents a major potential asset for the positive economic, social, and educational development of the Mayagüez urban area due to its unique, historic, and complex ecosystems with significant environmental and cultural resources. Of the identified stressors, urban expansion was considered as the most threatening. Hypothetical recommendations providing a base for future development and management efforts in the study area were formulated with the objective of protecting and sustainably using its resources, while promoting environmental and socioeconomic benefits in the region.  相似文献   

Cell biology. Precursor or charge supplier?     

GD Fairn  S Grinstein 《Science (New York, N.Y.)》2012,337(6095):653-654

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Genetic mapping provides evidence for the role of additive and non-additive QTLs in the response of inter-specific hybrids of <Emphasis Type="Italic">Eucalyptus</Emphasis> to <Emphasis Type="Italic">Puccinia psidii</Emphasis> rust infection     

Alexandre Alonso Alves  Carla Cristina Gonçalves Rosado  Danielle Assis Faria  Lúcio Mauro da Silva Guimarães  Douglas Lau  Sérgio Hermínio Brommonschenkel  Dario Grattapaglia  Acelino Couto Alfenas 《Euphytica》2012,183(1):27-38

Eucalypts are susceptible to a wide range of diseases. One of the most important diseases that affect Eucalyptus plantations worldwide is caused by the rust fungus Puccinia psidii. Here, we provide evidence on the complex genetic control of rust resistance in Eucalyptus inter-specific hybrids, by analyzing a number of full-sib families that display different patterns of segregation for rust resistance. These families are totally unrelated to those previously used in other inheritance studies of rust resistance. By using a full genome scan with 114 genetic markers (microsatellites and expressed sequence tag derived microsatellites) we also corroborated the existence and segregation of a resistance locus, explaining 11.5% of the phenotypic variation, on linkage group 3, corresponding to Ppr1. This find represents an additional validation of this locus in totally unrelated pedigree. We have also detected significant additive × additive digenic interactions with LOD >10.0 on several linkage groups. The additive and epistatic QTLs identified explain between 29.8 and 44.8% of the phenotypic variability for rust resistance. The recognition that both additive and non-additive genetic variation (epistasis) are important contributors to rust resistance in eucalypts reveals the complexity of this host-pathogen interaction and helps explain the success that breeding has achieved by selecting rust-resistant clones, where all the additive and non-additive effects are readily captured. The positioning of epistatic QTLs also provides starting points to look for the underlying genes or genomic regions controlling this phenotype on the upcoming E. grandis genome sequence.  相似文献   

Characterization of homologous and heterologous adaptive immune responses in porcine reproductive and respiratory syndrome virus infection     

Díaz I  Gimeno M  Darwich L  Navarro N  Kuzemtseva L  López S  Galindo I  Segalés J  Martín M  Pujols J  Mateu E 《Veterinary research》2012,43(1):30

The present study characterized the homologous and heterologous immune response in type-I porcine reproductive and respiratory syndrome virus (PRRSV) infection. Two experiments were conducted: in experiment 1, eight pigs were inoculated with PRRSV strain 3262 and 84 days post-inoculation (dpi) they were challenged with either strain 3262 or strain 3267 and followed for the next 14 days (98 dpi). In experiment 2, eight pigs were inoculated with strain 3267 and challenged at 84 dpi as above. Clinical course, viremia, humoral response (neutralizing and non-neutralizing antibodies, NA) and virus-specific IFN-γ responses (ELISPOT) were evaluated all throughout the study. Serum levels of IL-1, IL-6, IL-8, TNF-α and TGF-β were determined (ELISA) after the second challenge. In experiment 1 primo-inoculation with strain 3262 induced viremia of ≤ 28 days, low titres of homologous NA but strong IFN-γ responses. In contrast, strain 3267 induced longer viremias (up to 56 days), higher NA titres (≤ 6 log₂) and lower IFN-γ responses. Inoculation with 3267 produced higher serum IL-8 levels. After the re-challenge at 84 dpi, pigs in experiment 1 developed mostly a one week viremia regardless of the strain used. In experiment 2, neither the homologous nor the heterologous challenge resulted in detectable viremia although PRRSV was present in tonsils of some animals. Homologous re-inoculation with 3267 produced elevated TGF-β levels in serum for 7–14 days but this did not occur with the heterologous re-inoculation. In conclusion, inoculation with different PRRSV strains result in different virological and immunological outcomes and in different degrees of homologous and heterologous protection.  相似文献   

A new type of pterocarpanquinone that affects Toxoplasma gondii tachyzoites in vitro     

Portes Jde A  Netto CD  da Silva AJ  Costa PR  DaMatta RA  dos Santos TA  De Souza W  Seabra SH 《Veterinary parasitology》2012,186(3-4):261-269

Toxoplasma gondii, the agent of Toxoplasmosis, is an obligate intracellular protozoan able to infect a wide range of vertebrate cells, including nonprofessional and professional phagocytes. Therefore, drugs must have intracellular activities in order to control this parasite. The most common therapy for Toxoplasmosis is the combination of sulfadiazine and pyrimethamine. This treatment is associated with adverse reactions, thus, the development of new drugs is necessary. In previous studies, naphthoquinone derivatives showed anti-cancer activity functioning as agents capable of acting on groups of DNA, preventing cancer cells duplication. These derivatives also display anti-parasitic activity against Plasmodium falciparum and Leishmania amazonensis. The derivative pterocarpanquinone tested in this work resulted from the molecular hybridization between pterocarpans and naphtoquinone that presents anti-tumoral and anti-parasitic activities of lapachol. The aim of this work was to determine if this derivative is able to change T. gondii growth within LLC-MK2 cells. The drug did not arrest host cell growth, but was able to decrease the infection index of T. gondii with an IC(50) of 2.5 μM. Scanning and transmission electron microscopy analysis showed morphological changes of parasites including membrane damage. The parasite that survived tended to encyst as seen by Dolichos biflorus lectin staining and Bag-1 expression. These results suggest that pterocarpanquinones are drugs potentially important for the killing and encystment of T. gondii.  相似文献