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81.
82.
Ornamental kale (Brassica oleracea var. Acephala) is usually planted from early autumn until late winter. Since most of the plants used for phytoremediation cannot be grown during this time, kale can be a suitable option for phytoremediation and utilized during autumn and winter in urban landscape, especially in metropolitan areas where high levels of cadmium (Cd) and lead (Pb) pollutions exist. Kale growth in saline soil at different growth stages (germination and vegetative growth stages) was studied in this investigation. A factorial experiment based on completely randomized design (CRD) with four replications was used in this study. Treatments included three levels of sodium chloride (NaCl) (0, 30, and 60 mg/kg), four levels of Cd (0, 4, 8, and 16 mg/kg), and four levels of Pb (0, 1, 5, and 10 mg/kg). Results indicated that increase in Cd and Pb concentrations in the soil decreased fresh and dry weights of the plants. The results of the various growth stages revealed that under salinity stress, kale plants were able to absorb more Pb than Cd and effectively remediate Pb in polluted and saline lands. Cd accumulation in control treatment was 6.2% more than that in the saline treatments, whereas, Pb accumulation in the highest NaCl level, 60 mg/kg salinity treatment was 7.64% more than that of the control condition. Also, proline content of the plants was significantly increased under Cd and Pb stress. From the results of this study, it was concluded that using kale plant is recommended for phytoremediation of saline soils with 10 and 16 mg/kg Pb and Cd contents, respectively.  相似文献   
83.

Background:

The variable numbers of tandem-repeat (VNTR) alleles at the phenylalanine hydroxylase (PAH) gene have been used in carrier detection and prenatal diagnosis in phenylketonuria families. This study was carried out to analyze VNTR alleles at the PAH gene in Iranian Azeri Turkish population.

Methods:

In this study, 200 alleles from general population were studied by PCR.

Results:

The frequencies of VNTR alleles were 45%, 46%, 2%, 3%, 1%, and 3% in studied group regarding 3, 8, 9, 11, 12, and 13 repeat copies, respectively. Statistically significant differences were not found between expected and observed frequencies of VNTR genotypes (P > 0.05).

Conclusions:

VNTR alleles with three and eight repeats were frequent, and the VNTR alleles with 13 repeats showed 3% frequency in the tested group. This study is the first report on tested population genetic structure using VNTR alleles at the PAH gene. Key Words: Phenylalanine hydroxylase, Population genetics, Variable numbers of tandem-repeat  相似文献   
84.
Phytophthora niederhauserii, P. pisi, P. sojae and P. vignae are closely related species that are pathogenic to various legume plants. While P. sojae and P. vignae are reported to specifically infect soybean and cowpea, respectively, P. pisi is reported to attack pea and faba bean. Phytophthora niederhauserii is considered to have a broad host range. Zoospores of some Phytophthora species are chemotactically attracted to the isoflavones that are secreted by their host plants. The focus of the current study was to determine the chemotaxic behaviour of zoospores from closely related legume‐root infecting Phytophthora species and to investigate the correlation, if any, to host preference as determined by greenhouse pathogenicity tests. The results showed that P. sojae and P. vignae were attracted to the non‐soybean isoflavone prunetin as well as to the soybean isoflavones genistein and daidzein, which is in contrast with their host specificity on soybean and cowpea, respectively. On the other hand, P. pisi and P. niederhauserii were only attracted to prunetin, previously reported to be produced by pea, but not to the isoflavones associated with the non‐host soybean. The lack of responsiveness to genistein and daidzein in P. pisi may represent a recent adaptation to the host specialization towards pea. However, the affinity of P. niederhauserii to prunetin shows that this trait can also be present in taxa not specifically associated with legume hosts.  相似文献   
85.
This work was carried out to study the relationship between fish weight and natural food selectivity of Nile tilapia, Oreochromis niloticus (Linnaeus) reared in earthen ponds without supplementary diet and to guide the adaptation of feeding and fertilization strategies. Fish were stocked (1.5 fish/m2) in four fertilized earthen ponds (0.1 ha) for 6 months. Fifty fish were sampled monthly to estimate individual total weight and length and evaluate stomach contents. Fish specimens were categorized into different weight classes: <25 g, 25–50 g, 50–75 g, 75–100 g, and 100–125 g. Overall, the 50–75 g weight class had the highest degree of stomach fullness. The intestinal length-fish length ratio was similar across all fish weights, ranging from 2.9–3.4. Likewise, the composition of food items found in fish stomach was ranked as phytoplankton > detritus > zooplankton. Phytoplankton contribution to gut content increased with increasing fish weight, while detritus and zooplankton contribution decreased. Zooplankton never exceeded 1% of total stomach contents. Cyanobacteria, Chlorophyceae, Bacillariophyceae and Euglenophyceae mostly represented the phytoplankton. Bacillariophyceae dominated the phytoplanktonic portion of stomach contents at small fish size (<75 g/fish), while Chlorophyceae is the dominant group at large fish size (> 75 g/fish). Fish could select Cyanobacteria and Euglenophyceae at all fish weights, meanwhile Chlorophyceae and Bacillariophyceae were eaten with slight selectivity at larger weights..  相似文献   
86.
Research has shown that fresh fish is an extremely perishable food as compared to other food commodities. In this study, quality deterioration during storage of golden grey mullet (GGM) iced for 0, 6, and 12 h after landing was monitored. Microbiological indices (total viable count, TVC; psychrophilic counts, PTC; lactic acid bacteria, LAB; and Enterobacteriaceae counts, EBC), chemical parameters (pH; total volatile basic nitrogen, TVB-N; peroxide value, PV; thiobarbituric acid, TBA; and free fatty acids, FFA) values increased over the preicing duration. Delayed icing did not lead to significant increases (p > 0.05) in TVC, PTC, LAB, and EBC throughout the period of storage. All chemical indices increased during storage (p < 0.05). This study showed that sensorial analysis of GGM correlated well with microbiological analysis. Results of this study based on the microbiological and raw fish sensorial data indicated that the shelf life of GGM stored in ice immediately after catch was approximately 10–11 days, while the 6- and 12-h delays in icing shortened the shelf life to approximately 6–7 and 2–3 days, respectively.  相似文献   
87.
Multilayer microencapsulation of fish oil was evaluated by using fish gelatin (treated with or without microbial transglutaminase [MTGase]), chitosan, and maltodextrin for its ability to control lipid oxidation. This study showed that a mixture containing gelatin and maltodextrin obtained the highest percentage of core release. The oxidative stability of the formulated mixtures and the bulk oils was investigated during a period of 60 days. The peroxide value (PV) was assessed as a parameter for primary oxidation products, and p-anisidine (p-AV) and thiobarbituric acid reactive sub-stances (TBARS) was used to analyze secondary oxidation compounds. Observation of oxidation products showed that combinations of gelatin and maltodextrin made by adding MTGase and a mixture of gelatin and chitosan were able to increase the oxidative stability, and increases in PV and p-AV and TBARS were found for all oils.  相似文献   
88.
Quantitative and qualitative analyses of bacterial flora associated with pond water, gills, and intestine of polycultured healthy common carp (Cyprinus carpio) and African catfish (Clarias gariepinus) were carried out and identified to species level where possible. Total viable bacterial counts in the pond water ranged from 9.2?±?5.5?×?103 to 6.6?±?5.1?×?104 colony-forming units (cfu)/mL; in the gill filaments of carp and catfish, 3.3?±?3.8?×?106 to 7.9?±?5.6?×?106 and 1.1?±?4.6?×?105 to 2.3?±?5.2?×?106 cfu/g, respectively; and in the intestine of carp and catfish, 1.4?±?2.9?×?1010 to 1.7?±?6.0?×?1011 and 2.7?±?3.4?×?1010 to 1.0?±?4.5?×?1011 cfu/g, respectively. Gram-negative rod-shaped bacteria dominated the populations: 90% in carp, 89% in catfish, 80% in water, and 86% in the total populations. Altogether, 14 bacterial species of 10 genera were identified in total populations. Pond water bacteria had a reflection on the bacterial composition of the gills and intestine of carp and catfish. Aeromonas hydrophila, Shewanella putrefaciens, Vibrio cholerae, Staphylococcus sp., and Vibrio vulnificus appeared as the common bacteria in the populations, where the first three were highly significantly abundant (P?<?0.0001). Moreover, A. hydrophila was the most significantly dominant bacteria (32%; P?<?0.005) among the total populations. Pantoea sp. and Pasteurella pneumotropica were present only in carp and catfish, respectively, but Corynebacterium urealyticum and Micrococcus sp. were present only in pond water.  相似文献   
89.
Effect of dietary tryptophan (TRP) on copper toxicity was investigated in common carp, Cyprinus carpio. Fish were randomly distributed into two triplicate treatments (control and TRP) and fed control (TRP?=?3.1?g?kg(-1)) or TRP (TRP?=?8.1?g?kg(-1)) diets over a 2-week period. Then, both treatments were subjected to 10?mg?l(-1) copper sulfate over a 7-day period. Mortality and serum cortisol, glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), sodium, potassium, and chloride levels were recorded at 0, 24, 72, 120, and 168?h post-copper exposure. There was no mortality in the TRP group, whereas the control group mortality was started at 120?h post-copper exposure (21.7?±?7.6?%) and reached 61.7?±?10.4?% at the end of the experiment. Cortisol, glucose, ALT, and AST levels significantly (P?相似文献   
90.
Oocyte maturation in fish is a hormonally regulated process. In the light of long-term oocyte maturation in beluga, the aim of this research was to study the estrogenic effects of different concentrations of soy dietary genistein (GE) and equol (EQ) on the growth performance and ovary development in farmed female Huso huso. Fish were fed with concentrations 0 (control), 0.2, 0.4, 0.8 and 1.6 g of EQ and GE per kg of isoproteic (CP 45 %) and isoenergetic (19.5 MJ/kg) diets during a year. Blood samples and ovary biopsies were collected from each fish seasonally. The main results of the present experimentation are that growth performance was not affected significantly both in GE and EQ (P > 0.05). EQ at concentration 0.4 g/kg had more estrogenic effects than other concentrations of EQ and GE in beluga so that 64 % of fish were matured sexually. Some reproductive indices such as oocyte diameter, testosterone (T) and 17β-estradiol (E2) increased significantly at EQ 0.4 g/kg at the end of experiment (P < 0.05), while 17α-hydroxy progesterone level (17α-OHP) showed no significant changes at all concentrations. Biochemical indices such as calcium, phosphorous and cholesterol increased at GE concentrations, but decreased at EQ concentrations similarly at the end of experiment. There was a negative relationship between plasma phosphorous and alkaline phosphatase enzyme levels. Based on results, EQ at concentration 0.4 g/kg improved oocyte development more than the other concentrations of GE and EQ, and therefore, it can be used as an additive to diets for inducing ovary development in this species.  相似文献   
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