Essential role of Fkbp6 in male fertility and homologous chromosome pairing in meiosis     

Crackower MA  Kolas NK  Noguchi J  Sarao R  Kikuchi K  Kaneko H  Kobayashi E  Kawai Y  Kozieradzki I  Landers R  Mo R  Hui CC  Nieves E  Cohen PE  Osborne LR  Wada T  Kunieda T  Moens PB  Penninger JM 《Science (New York, N.Y.)》2003,300(5623):1291-1295

Meiosis is a critical stage of gametogenesis in which alignment and synapsis of chromosomal pairs occur, allowing for the recombination of maternal and paternal genomes. Here we show that FK506 binding protein (Fkbp6) localizes to meiotic chromosome cores and regions of homologous chromosome synapsis. Targeted inactivation of Fkbp6 in mice results in aspermic males and the absence of normal pachytene spermatocytes. Moreover, we identified the deletion of Fkbp6 exon 8 as the causative mutation in spontaneously male sterile as/as mutant rats. Loss of Fkbp6 results in abnormal pairing and misalignments between homologous chromosomes, nonhomologous partner switches, and autosynapsis of X chromosome cores in meiotic spermatocytes. Fertility and meiosis are normal in Fkbp6 mutant females. Thus, Fkbp6 is a component of the synaptonemal complex essential for sex-specific fertility and for the fidelity of homologous chromosome pairing in meiosis.  相似文献   

A broad spectrum,one-step RT-PCR to detect <Emphasis Type="Italic">Satsuma dwarf virus</Emphasis> variants using universal primers targeting both segmented RNAs 1 and 2     

Shin-ichi Shimizu  Takao Ito  Takanori Miyoshi  Yasunobu Tachibana  Tsutae Ito 《Journal of General Plant Pathology》2011,77(6):326-330

Universal primers to detect Satsuma dwarf virus (SDV), including distantly related strains Citrus mosaic virus (CiMV), Navel orange infectious mottling virus (NIMV), and Hyuganatsu virus (HV), were tested in a convenient one-step RT-PCR assay. SDV was the most broadly detected using uSDVup/uSDVdo primers that specifically targeted a nucleotide sequence in the 3′-noncoding region that is conserved in both segmented RNAs 1 and 2 of SDV among the tested primers. Nucleotide sequence analysis confirmed that the amplified RT-PCR products could be derived from RNAs 1 or 2 of SDV variants, some of which had interesting genetic diversity.  相似文献   

Growth of the collembolan Folsomia candida Willem in soil supplemented with glucose   总被引：1，自引：0，他引：1  

Satoshi Kaneda  Nobuhiro Kaneko   《Pedobiologia》2004,48(2):165-170

Freely available glucose improves the conditions for soil microorganisms which are utilized as food by Collembola. We examined the effects of glucose application on collembolan (Folsomia candida Willem) growth and on several biotic and abiotic soil parameters (microbial biomass, soil respiration, qCO₂, dissolved organic carbon, inorganic nitrogen, and Olson-P) in an artificial system without predatory pressure on Collembola. Glucose addition increased soil respiration and qCO₂, and decreased nutrient levels in the soil. Collembolan growth increased with increasing glucose doses. We conclude that the availability of carbon substrates can sustain collembolan growth via an improvement of microbial growth conditions.  相似文献   

Ascorbate regeneration by the reduced form of 2-amino-3-carboxy-1, 4-naphthoquinone, a strong growth stimulator for bifidobacteria     

Yamazaki S  Iwasa K  Kano K  Ikeda T  Taketomo N  Kaneko T 《Journal of agricultural and food chemistry》2000,48(11):5643-5648

Nonenzymatic reduction of dehydroascorbate into ascorbate by the reduced form (quinol form) of 2-amino-3-carboxy-1,4-naphthoquinone, a strong growth stimulator for bifidobacteria, has been found. The bimolecular reaction rate constant was evaluated as 9 M(-)(1) s(-)(1) at pH 7.0. This reaction has been successfully coupled with enzymatic regeneration of the naphthoquinol by NAD(P)H in cell-free extracts of Bifidobacterium longum 6001. The overall reaction is a regeneration of NAD(P)(+) by dehydroascorbate [or a regeneration of ascorbate by NAD(P)H], in which the naphthoquinone/quinol redox couple functions as an electron transfer mediator. Kinetic study of the reduction of dehydroascorbate with related quinol compounds suggested the significance of the amino substituent of the naphthoquinol. A mechanism of the electron transfer from the quinol to dehydroascorbate is proposed, where the first step of the reaction is a nucleophilic addition of the C(2)-amino substituent of the naphthoquinol to the C(2)-position of dehydroascorbate to form a Schiff base intermediate.  相似文献   

Heinz body hemolytic anemia associated with the use of urinary antiseptics containing methylene blue in the cat   总被引：1，自引：0，他引：1  

R D Schechter  O W Schalm  J J Kaneko 《Journal of the American Veterinary Medical Association》1973,162(1):37-44

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Physicochemical properties of Ibaraki virus hemagglutinin     

S Tokuhisa  Y Inaba  K Sato  Y Miura  N Kaneko  M Matumoto 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1983,45(5):637-646

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Book reviews     

J. J. Kaneko DVM  PhD  G. M. Robins 《Veterinary research communications》1987,11(2):199-200

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Protection of mice against parenteral and oral infection with Yersinia enterocolitica     

R Nakajima  K Kaneko  N Hashimoto 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1984,46(5):721-727

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Postprandial changes in serum bile acids concentration and fractionation of individual bile acid by high performance liquid chromatography in normal dogs     

T Washizu  I Koizumi  J J Kaneko 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(4):593-600

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Glucose tolerance and insulin response in diabetes mellitus of dogs     

J J Kaneko  D Mattheeuws  R P Rottiers  A Vermeulen 《The Journal of small animal practice》1978,19(2):85-94

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