Identification and validation of a quantitative trait locus associated with wheat yellow mosaic virus pathotype I resistance in a Japanese wheat variety          下载免费PDF全文

Hisayo Kojima  Zenta Nishio  Fuminori Kobayashi  Mika Saito  Takahide Sasaya  Chikako Kiribuchi‐Otobe  Masako Seki  Shunsuke Oda  Toshiki Nakamura 《Plant Breeding》2015,134(4):373-378

Yellow mosaic disease, caused by wheat yellow mosaic virus (WYMV), is one of the most serious diseases of winter wheat (Triticum aestivum L.) in Japan. The three pathotypes of WYMV are distributed in different geographical areas: pathotype I is found mainly in western and central Japan (Kanto), pathotype II in northern Japan (Tohoku and Hokkaido) and pathotype III on the southern island of Japan (Kyushu). A total of 246 doubled‐haploid (DH) lines, derived from a cross between ‘Yumechikara’ (resistant) and ‘Kitahonami’ (susceptible), were evaluated for 2 years for their resistance to WYMV pathotype I. A single major quantitative trait locus, Q.Ymym, mapping to chromosome 2D was associated with resistance to pathotype I in ‘Yumechikara’. This is the first time a QTL responsible for pathotype I resistance has been identified. Fine mapping of Q.Ymym indicated that it was on a tight linkage block originating from ‘Yumechikara’, and the markers associated with this block will accelerate the development of varieties resistant to WYMV pathotype I.  相似文献   

Antibacterial activity of moso bamboo shoot skin (<Emphasis Type="Italic">Phyllostachys pubescens</Emphasis>) against <Emphasis Type="Italic">Staphylococcus aureus</Emphasis>     

Akinobu Tanaka  Hyo Jung Kim  Shojiro Oda  Kuniyoshi Shimizu  Ryuichiro Kondo 《Journal of Wood Science》2011,57(6):542-544

This study examined the antibacterial activity of moso bamboo shoot skin (Phyllostachys pubescens). Bamboo shoot skin itself and its dichloromethane extract had antibacterial activity against Staphylococcus aureus. Results suggest the possibility of effective utilization of antibacterial materials from bamboo shoot skins that are mainly discarded at present.  相似文献   

Evaluation of serum and urine 1,5-anhydro-D-glucitol and myo-inositol concentrations in healthy dogs     

Miki Y  Mori A  Hayakawa N  Niki T  Oda H  Saeki K  Sato T  Tazaki H  Ishioka K  Arai T  Sako T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1117-1126

1,5-anhydro-D-glucitol (1,5AG) is a pyranoid polyol compound found in human circulating blood. Myo-inositol (MI) is a stereoisomer of inositol and serves as a precursor of inositol phospholipids. 1,5AG and MI are filtered by the glomerulus and almost completely reabsorbed through the renal tubules. However, under hyperglycemic conditions, reabsorption through the renal tubules is competitively inhibited because the structures of 1,5AG and MI resemble that of glucose. In this study, we investigated the kinetics of serum and urine 1,5AG and MI levels in healthy dogs. We demonstrated that 1,5AG and MI exist in canine serum and urine by gas chromatography-mass spectrometry. Under continuous hyperglycemic conditions, the serum 1,5AG concentration in healthy dogs decreased while the serum MI concentration remained unchanged. Urinary excretion of 1,5AG and MI increased significantly after blood glucose concentrations reached 200 to 220 mg/dl. A significant negative correlation was observed between serum 1,5AG and glucose concentrations during hyperglycemia. However, no significant correlation was observed between serum MI and glucose concentrations. In this study, we demonstrated that serum and urine 1,5AG and MI levels were changed by blood glucose concentrations. The serum 1,5AG concentration was decreased by continuous hyperglycemia. However, the serum MI concentration does not reflect hyperglycemia.  相似文献   

Isolation of equine rotavirus in cell cultures from foals with diarrhea     

H Imagawa  R Wada  K Hirasawa  Y Akiyama  T Oda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1984,46(1):1-9

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Characteristics of developmental changes in the kinetics of glucose and urea in Japanese Black calves: comparison with Holstein calves     

Shingu H  Hayashi H  Touno E  Oshibe A  Kushibiki S  Oda S  Katoh K  Obara Y 《Journal of animal science》2007,85(11):2910-2915

The current study was conducted to clarify the characteristics of glucose and urea kinetics in male Japanese Black calves, using a glucose and urea dilution method with stable isotopes, at preweaning (3 wk of age) and postweaning (13 and 26 wk of age) stages, in comparison with the kinetics of glucose and urea in male Holstein calves. Six Japanese Black and 6 Holstein calves were arranged in a 2 (breed) x 3 (stage) factorial block design. These 12 suckling calves were fed only whole milk, offered concentrate and or chardgrass hay after 3 wk of age, and weaned at 6 wk of age. Under steady-state conditions, glucose challenges (1.0 mg/kg of BW; [U-13C]d-glucose) and urea challenges (both 2.2 mg/kg of BW; [13C]urea and [15N2]urea) were performed at the 3 stages examined. There were no significant differences in plasma glucose concentrations between Japanese Black and Holstein calves at any stage, but the glucose concentrations at 3 wk of age were greater (P <0.05) than those at 13 wk of age in both breeds. The glucose pool size in Japanese Black calves was smaller (P <0.05) than that in Holstein calves at all stages. Within each breed, there were no significant differences between the glucose pool sizes at 3 and 13 wk of age, but the glucose pool size was larger (P <0.05) at 26 wk of age. Moreover, Japanese Black calves had greater glucose irreversible loss rates (P <0.01) than Holstein calves at 3 wk of age, and the glucose irreversible loss rates were less (P <0.05) on and after 13 wk of age in both breeds. Japanese Black calves had greater plasma urea N concentrations (P <0.05) than Holstein calves at all stages, and in both breeds, the urea N concentrations increased (P <0.05) with age. The urea pool size was smaller (P <0.01) in Japanese Black calves than in Holstein calves at all stages, and in both breeds, the urea pool size increased (P <0.05) with age. In comparison with Holstein calves, Japanese Black calves had greater urea irreversible loss rates (P <0.05) on and after 13 wk of age and greater urea recycling rates (P <0.05) at 26 wk of age. In addition, in both breeds, urea irreversible loss rates and urea recycling rates increased (P <0.05) with age. We conclude that Japanese Black calves have partially different glucose and urea kinetics from Holstein calves and that the kinetics of these metabolites in both Japanese Black and Holstein calves are strongly influenced by weaning.  相似文献   

Ascophyllan Purified from Ascophyllum nodosum Induces Th1 and Tc1 Immune Responses by Promoting Dendritic Cell Maturation     

Wei Zhang  Jiang-Yuan Du  Zedong Jiang  Takasi Okimura  Tatsuya Oda  Qing Yu  Jun-O Jin 《Marine drugs》2014,12(7):4148-4164

Marine-derived sulfated polysaccharides have been shown to possess certain anti-virus, anti-tumor, anti-inflammatory and anti-coagulant activities. However, the in vivo immunomodulatory effects of marine-derived pure compounds have been less well characterized. In this study, we investigated the effect of ascophyllan, a sulfated polysaccharide purified from Ascophyllum nodosum, on the maturation of mouse dendritic cells (DCs) in vitro and in vivo. Ascophyllan induced up-regulation of co-stimulatory molecules and production of pro-inflammatory cytokines in bone marrow-derived DCs (BMDCs). Moreover, in vivo administration of ascophyllan promotes up-regulation of CD40, CD80, CD86, MHC class I and MHC class II and production of IL-6, IL-12 and TNF-α in spleen cDCs. Interestingly, ascophyllan induced a higher degree of co-stimulatory molecule up-regulation and pro-inflammatory cytokine production than fucoidan, a marine-derived polysaccharide with well-defined effect for promoting DC maturation. Ascophyllan also promoted the generation of IFN-γ-producing Th1 and Tc1 cells in the presence of DCs in an IL-12-dependent manner. Finally, myeloid differentiation primary response 88 (MyD88) signaling pathway was essential for DC maturation induced by ascophyllan. Taken together, these results demonstrate that ascophyllan induces DC maturation, and consequently enhances Th1 and Tc1 responses in vivo. This knowledge could facilitate the development of novel therapeutic strategies to combat infectious diseases and cancer.  相似文献   

EDEM as an acceptor of terminally misfolded glycoproteins released from calnexin     

Oda Y  Hosokawa N  Wada I  Nagata K 《Science (New York, N.Y.)》2003,299(5611):1394-1397

Terminally misfolded proteins in the endoplasmic reticulum (ER) are retrotranslocated to the cytoplasm and degraded by proteasomes through a mechanism known as ER-associated degradation (ERAD). EDEM, a postulated Man8B-binding protein, accelerates the degradation of misfolded proteins in the ER. Here, EDEM was shown to interact with calnexin, but not with calreticulin, through its transmembrane region. Both binding of substrates to calnexin and their release from calnexin were required for ERAD to occur. Overexpression of EDEM accelerated ERAD by promoting the release of terminally misfolded proteins from calnexin. Thus, EDEM appeared to function in the ERAD pathway by accepting substrates from calnexin.  相似文献   

Management of 1,3-dichloropropene in pineapple for efficacy and reduced volatile losses     

R. C. Schneider  B. S. Sipes  C. H. Oda  R. E. Green  D. P. Schmitt 《Crop Protection》1995,14(8):611-618

Two application methods, single- and double-chisel injection of the fumigant nematicide 1,3-dichloropropene (1,3-D) were evaluated for efficacy and environmental fate in pineapple field experiments. The objectives were to compare the 1,3-D soil distribution between injection methods, evaluate volatile losses to the atmosphere, and to determine efficacy with each method. In a small-plot field experiment, 1,3-D (224, 337, and 393 1 ha⁻¹) was manually injected 30 cm deep with fumiguns to simulate the commercial practice of a single- or double-chisel injection per planting bed. 1,3-D concentrations in the soil atmosphere and in soil samples were determined and compared to nematode control. In two large-scale field experiments, 1,3-D (224 1 ha⁻¹) was applied with commercial injection equipment with and without polyethylene mulch. Air monitoring for 1,3-D showed a reduction in 1,3-D air emissions with single-chisel injection compared with double-chisel injection. In all experiments, the two injection methods resulted in equivalent 1,3-D concentrations in the plant line. Single-chisel injection resulted in improved retention of 1,3-D within the planting bed as shown by low 1,3-D soil concentrations in the interbed. Nematode counts and bioassay studies found equivalent nematode control in the planting bed with either injection method. The single-chisel method, however, resulted in reduced nematode control in the interbed region.  相似文献   

Isolation of Araguspongine M,a New Stereoisomer of an Araguspongine/Xestospongin alkaloid,and Dopamine from the Marine Sponge Neopetrosia exigua Collected in Palau     

Hongwei Liu  Yuri Mishima  Takeshi Fujiwara  Hiroshi Nagai  Akira Kitazawa  Yuji Mine  Hisayoshi Kobayashi  Xinsheng Yao  Junko Yamada  Taiko Oda  Michio Namikoshi 《Marine drugs》2004,2(4):154-163

A new stereoisomer of an araguspongine/xestospongin alkaloid, named araguspongine M (1), has been isolated together with 12 known compounds, araguspongines B (2) and D (3), dopamine, three galactosyl diacylglycerols, 24-methyl cholesterol, 5,6-dihydrocholesterol, β-sitosterol, and three 5α,8α-epidioxy sterols (11–13), from the marine sponge Neopetrosia exigua (formerly Xestospongia exigua) collected in Palau. The structure of 1 was assigned on the basis of its spectral data analysis. This is the first report on the isolation of dopamine from a marine sponge. This compound may be produced by an endosymbiotic Synechococcus-like cyanobacterium. Compounds 1–3 and 11–13 showed cytotoxicity against HL-60 at IC₅₀’s of 5.5, 5.5, 5.9, 22.4, 9.5, and 9.6 μM, respectively. The possible biosynthesis origin of the isolated metabolites is discussed.  相似文献   

Absorption,translocation, and assimilation of foliar-applied urea compared with nitrate and ammonium in tomato plants     

Xue Wen Tan  Masayuki Oda 《Soil Science and Plant Nutrition》2013,59(3):609-616

To evaluate the use of foliar application of N fertilizer and the occurrence of leaf injury in tomato plants (Lycopersicon esculentum Mill., cv. Momotaro), the effects of the form and concentration of N and solution pH on the leaf injury were studied in the first experiment (Expt. 1). The effects of solution pH and leaf surface on the absorption, translocation, and assimilation of urea were compared with those of nitrate and ammonium in the second experiment (Expt. 2). In Expt. 1, no leaf injury was observed regardless of N sources applied at the N concentration of 1.0 g L^-1. Compared with nitrate or ammonium, the index of leaf injury was the lowest in the leaf to which urea had been applied (hereafter referred to as “urea-applied leaf”), when the N level increased from 2.0 to 10.0 g L^-1. Leaf injury was not affected by the solution pH in the case of urea, but it increased in the case of ammonium and decreased when nitrate was applied with increasing solution pH. In Expt. 2, the absorption of nitrate and ammonium by a leaf within 4 d was 34% and 74% of that of urea, respectively. N absorption at the lower leaf surface was much greater than that at the upper leaf surface for each N source. No apparent effect of solution pH on the absorption of urea was detected. With increasing solution pH, however, the absorption of nitrate decreased. The absorption of ammonium was the greatest at solution pH 7.5. Total-¹⁵N translocation from applied leaf to other plant parts within 4 d was the largest in the urea-applied plants. Effects of solution pH and leaf surface on ¹⁵N distribution were not appreciable. ¹⁵N assimilation was the quickest in the urea-applied plants. Two days after application, ¹⁵N assimilation in the whole plant was up to 76.9% in the urea-applied plants, but only 33.7% and 43.0% in the nitrate- and ammonium-applied plants, respectively. Urea was an appropriate foliar N source due to the low ability to injure foliage because of the rapid absorption and translocation, fast assimilation, and the wide and suitable range of solution pH. Foliar application of N to the lower leaf surface was recommended.  相似文献