Oocyte transport: Developmental competence of bovine oocytes arrested at germinal vesicle stage by cycloheximide under air     

Hashimoto S  Kimura K  Iwata H  Takakura R 《The Journal of reproduction and development》2003,49(1):61-66

The effects of the medium (TCM 199 or SOFaa) and temperature (20 or 39 C) during meiotic arrest by cycloheximide (CHX) under air on the developmental competence of bovine oocytes after in vitro maturation (IVM) and fertilization (IVF) were investigated. Oocytes were maintained in meiotic arrest by 10 microg/ml CHX in a 50-microl droplet of 25-mM HEPES-buffered TCM 199 (H199) at 39 C or synthetic oviduct fluid (HSOFaa) at 20 or 39 C in air for 24 h. After release from the arrest, the oocytes was matured and fertilized in vitro and their developmental competence was examined. The developmental rate of oocytes arrested in HSOFaa at 20 C to the blastocyst stage was similar to that of non-arrested oocytes but was significantly higher (P<0.05) than that of oocytes arrested at 39 C in H199 or in HSOFaa. In consideration of oocyte transport conditions, we also investigated the meiotic arrest of oocytes maintained in a 0.25-ml straw by CHX individually with 10 microl HSOFaa or as a group (40-50 oocytes) with 170-200 microl HSOFaa at 20 C in air for 24 h. After release from meiotic arrest, the developmental competence of these oocytes was assessed similarly. The developmental rate of oocytes treated with CHX individually was similar to that of those treated with CHX in 50-microl droplet of HSOFaa at 20 C. However, the developmental rate of oocytes treated with CHX as a group was lower than that of oocytes treated with CHX in a 50-microl droplet. Five blastocysts developed from oocytes maintained in meiotic arrest in a plastic straw were transferred to five recipient heifers. Consequently, three recipients became pregnant and 2 calves were delivered. The results of the present study indicate that bovine oocytes treated with CHX in HSOFaa at 20 C under air retain the same developmental competence as non-arrested oocytes.  相似文献   

茶秆竹高生长模型的比较及组合选择   总被引：2，自引：1，他引：1  

代全林  陈存及  肖书平  陈东阳  董建文 《福建林学院学报》2002,22(2):129-132

通过对 5种 S增长模型的比较及组合选择 ,结果表明 :S增长模型都具有单调性、渐近性和拐点 ,此特征能客观地反映诸多自然和经济规律 .通过一定的加权组合 ,用多个增长模型来反映 S变化过程 ,可以提高拟合的精度 ,多个模型的组合要优于单个模型 .用加权模型可以很好地反映茶秆竹的高生长过程 ,在实际生产中可选择 yp2 或 yp 3 组合  相似文献   

Effects of various adjuvants on efficacy of a vaccine against Streptococcus bovis and Lactobacillus spp in cattle     

Shu Q  Hillard MA  Bindon BM  Duan E  Xu Y  Bird SH  Rowe JB  Oddy VH  Gill HS 《American journal of veterinary research》2000,61(7):839-843

OBJECTIVE: To determine efficacy of vaccines incorporating QuilA, alum, dextran combined with mineral oil, or Freund adjuvant for immunization of feedlot cattle against Streptococcus bovis and Lactobacillus spp. ANIMALS: 24 steers housed under feedlot conditions. PROCEDURE: Steers were randomly assigned to 4 experimental groups and a control group. Animals in experimental groups were inoculated on days 0 and 26 with vaccines containing Freund adjuvant (FCA), QuilA, dextran combined with mineral oil (Dex), or alum as adjuvant. Serum anti-S bovis and anti-Lactobacillus IgG concentrations were measured, along with fecal pH, ruminal fluid pH, and number of S bovis and Lactobacillus spp in ruminal fluid. RESULTS: Throughout the study, serum anti-S bovis and anti-Lactobacillus IgG concentrations for animals in the Dex, QuilA, and alum groups were similar to or significantly higher than concentrations for animals in the FCA group. Serum anti-S bovis and anti-Lactobacillus IgG concentrations were significantly increased on days 26 through 75 in all 4 experimental groups, and there was a linear relationship between anti-S bovis and anti-Lactobacillus IgG concentrations. For animals in the QuilA and Dex groups, mean pH of feces throughout the period of experiment were significantly higher and numbers of S bovis and Lactobacillus spp in ruminal fluid on day 47 were significantly lower than values for control cattle. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that immunization of feedlot steers against S bovis and Lactobacillus spp with vaccines incorporating Freund adjuvant, QuilA, dextran, or alum as an adjuvant effectively induced high, long-lasting serum anti-S bovis and anti-Lactobacillus IgG concentrations. Of the adjuvants tested, dextran may be the most effective.  相似文献   

套袋对红富士苹果果皮细胞超微结构的影响   总被引：19，自引：0，他引：19  

李秀菊  刘用生  束怀瑞 《园艺学报》2000,27(3):202-204

红富士苹果果皮细胞超微结构的观察结果表明,未套袋颗粒果皮表层细胞叶绿体片层结构发育较套袋果实发达,至果实采收前,未套袋果液泡染色较深县均匀,而套袋果液泡染色深,呈絮状凝结,这可能是颗粒去袋后花青苷迅速合成,在液泡内大量积累所致。  相似文献   

半胱亚磺酸脱羧酶(CSD)基因在大鼠睾丸组织中的表达及其序列分析   总被引：1，自引：0，他引：1  

杨建成  林淑梅  栾新红  胡建民 《黑龙江畜牧兽医》2007,(6):6-8

试验根据大鼠肝脏牛磺酸生物合成关键酶——半胱亚磺酸脱羧酶(CSD)基因已知核苷酸序列,设计了1对特异的寡核苷酸引物,对提取的睾丸组织总RNA进行RT-PCR扩增,扩增出468 bp的DNA片段,在国际上首次证明CSD基因在大鼠睾丸组织中存在表达,即牛磺酸可在大鼠睾丸组织中生物合成。测定了扩增的睾丸组织CSD基因核苷酸序列,并与已知肝脏CSD基因核苷酸序列及其对应的氨基酸序列作了同源性分析。结果表明:睾丸组织CSD基因核苷酸序列同已知肝脏CSD基因核苷酸序列的同源性为99.8%,其对应氨基酸序列的同源性为100%。  相似文献   

萧山草三黄鸡屠宰性能和肉质分析     

舒琦艳  卢立志  原爱平  沈军达  王得前  陶争荣  李国勤  袁青妍  赵爱珍  徐坚 《中国家禽》2006,28(15):19-20

研究对70日龄萧山草三黄鸡进行了屠宰性能和肉质性状的测定,结果显示:公鸡活重、屠体重、半净膛率和全净膛率优于母鸡,差异显著(P<0.05)。腿肌的肉色OD值、剪切力和pH值均大于胸肌,系水力小于胸肌,差异均显著(P<0.05);水分含量、粗脂肪含量大于胸肌,粗蛋白含量、粗灰分含量小于胸肌,差异均显著(P<0.05)。研究表明母鸡的肉质优于公鸡,屠宰性能则不及公鸡。  相似文献   

苍耳等药用植物提取物对小菜蛾的拒食作用和产卵忌避效果   总被引：16，自引：0，他引：16  

周琼  刘炳荣  舒迎花  何艳 《中国蔬菜》2006,1(2):17-20

室内采用叶碟法,研究苍耳(Xanthium sibiricumPetril)等8种药用植物乙醇提取物对小菜蛾(Plutel-la xylostella)的拒食和产卵忌避活性。结果表明,苍耳(果实)、雷公藤(根)和商陆(根)乙醇提取物对小菜蛾4龄幼虫的取食均有较强的抑制作用,选择性拒食率分别为86.16%、49.06%和43.82%;非选择性拒食效果仍以苍耳(果实)提取物最好,达45.23%。选择性产卵忌避率以雷公藤(根)提取物为最高(69.52%),其次是苍耳(果实)和山苍树(枝叶)提取物,分别为62.50%和57.72%,雷公藤(根)、商陆(根)、窃衣(茎叶)和苍耳(果实)的非选择性产卵忌避作用均在77%以上。苍耳和雷公藤提取物在对小菜蛾的拒食和产卵忌避的各测试中,均表现较强活性。  相似文献   

套袋对苹果生产投资结构的影响及密植园遮光问题   总被引：7，自引：0，他引：7  

翟衡  任诚  厉恩茂  史大川  林桂雁  束怀瑞 《园艺学报》2006,33(4):921-926

 套袋技术目前已经广泛应用于果树生产, 特别是富士苹果套袋率在山东和陕西主产区超过75% , 每亩套袋量8 000～18 000个, 纸袋投资270～1 100元, 占生产总投入比重的1 /3, 占生产资料平均投入的45%; 而套袋和摘袋雇工成本在人工成本中占主要部分, 套袋对苹果生产投资结构产生了巨大影响。对乔砧密植园大量套袋苹果树进行光合测定发现, 套袋造成了投影部位5片以上叶子遮光, 无论树冠外围还是内膛, 被遮光的叶片净光合速率均为负值, 大量套袋极显著降低了树冠中部和内膛照光叶片的光合能力。有关果实套袋技术的应用应该根据市场和生产条件细分确定。  相似文献   

Relationship between uPA and NF-κB p65 expression and its clin ical significance in breast cancer     

WANG Hong  WU Jin-min  CAO Hou-jun  ZHOU Wen-peng  FANG Yong  ZHANG Xing  ZHENG Shu 《园艺学报》2006,22(6):1218-1221

AIM:To evaluate the relationship between u PA and NF-κB p65 expression and its clinical significance in breast cancer.METHODS:The uPA mRNA was measured by real-time fluorescent quan t itative PCR in 46 cases of breast cancer tissues and their adjacent counterparts .NF-κB p65 were measured using immunohistochemistry.RESULTS:The expression of uPA gene was elevated in 63% of cases ,and there was a strong correlation between NF-κB p65 and uPA expression (r ＝0.451，P<0.01).In addition,the expression of uPA was associated with num bers of axillary lymph node involvement and tumor size.However,no significance was found in uPA expression in the age,pathological type and distant metastasi s organ classification.CONCLUSION:The uPA gene is highly expressed in most of the brea st cancer,and there is strong correlation to NF-κB p65 expression,number of l ymph nodes involvement and tumor size.  相似文献   

低温胁迫对东方山羊豆幼苗生理特性的影响     

沙伟  师帅  罗新义  荣姝 《北方园艺》2007,(11):59-60

以东方山羊豆及敖汉苜蓿为材料,研究低温胁迫对东方山羊豆和敖汉苜蓿可溶性蛋白含量、保护酶活性、渗透调节物质的影响.结果表明:在低温胁迫下,东方山羊豆及敖汉苜蓿脯氨酸含量、SOD、POD活性、可溶性蛋白含量先上升后下降.经对几项生理指标的综合分析,认为东方山羊豆比敖汉苜蓿具较强的抗低温能力.  相似文献