Development of 18 microsatellite markers in Pieris japonica,a poisonous tree insulated from the browsing pressure of herbivores,using a next-generation sequencer     

Hiroyuki Kurokochi  Engkong Tan  Shuichi Asakawa  Chunlan Lian 《Journal of Forest Research》2015,20(1):244-247

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Stocking effectiveness of hatchery-released kuruma prawns estimated by two-stage sampling of commercial catch in Ariake Sound, Japan     

Tadanori Yamaguchi  Shiro Ito  Katsuyuki Hamasaki  Shuichi Kitada 《Fisheries Science》2006,72(2):233-238

ABSTRACT:    To evaluate the stocking effectiveness of the kuruma prawn Penaeus japonicus , a two-stage sampling survey of Gensiki bottom-drift-netters was carried out in Ariake Sound, Saga Prefecture, Japan. A total of 471 000 hatchery-produced juveniles, marked by cutting off the right uropods, was released. Thirty-three fishing days were randomly drawn from four boats, which were selected from the 80 boats operating in the fishery. All prawns caught on the selected days were examined, and the hatchery-produced individuals were identified by their regenerated uropods. A telephone survey of all boats was also conducted, to determine the number of fishing days. A total of 286 marked prawns were caught on the survey days for the Saga area in Ariake Sound, from the last half of July to the first half of September 2002. The recapture rate and standard error were estimated at 0.76 ± 0.52%. Economic efficiency was estimated at 0.28 ± 0.02. Strategies for sampling schemes and stock recovery are also discussed.  相似文献   

Short Paper     

Chutima Khomvilai  Shuichi Karita  Masaaki Kashiwagi  Motoi Yoshioka 《Fisheries Science》2005,71(5):1188-1190

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防火林带营造木荷火力楠混交林生长效果分析   总被引：1，自引：0，他引：1  

方水池 《绿色科技》2014,(8):95-97

对马尾松低产林改造,营造防火林带木荷火力楠生长效果进行了调查,发现林木生长良好。结果显示：经过14a的培育,其林木生长不同,木荷火力楠混交林的林分生长量,比木荷、火力楠纯林的林分生长量更具优势,平均树高、胸径、树冠、枝下高、郁闭度、单株材积、蓄积量,分别比木荷、火力楠纯林林分平均树高大13.54%、8.55%,胸径粗18.39%、14.21%,树冠大8.24%、22.26%,枝下高8.75%、17.67%,郁闭度大2.13%、3.23%,单株材积大45.43%、41.18%,蓄积量大54.95%、37.54%。土壤肥力N、P、K分别提高0.9%、1.92%、1.79%,1.80%、6.00%、3.64%。木荷火力楠混交林能涵养水源、保持水土,有效控制林火蔓延,可减少森林火灾和灾后损失。  相似文献   

Polymerase chain reaction (PCR) amplification of bacilliform virus (RV-PJ) DNA in Penaeus japonicus Bate and systemic ectodermal and mesodermal baculovirus (SEMBV) DNA in Penaeus monodon Fabricius   总被引：3，自引：0，他引：3  

Y Takahashi  T Itami  M Maeda  N Suzuki  J Kasornchandra  K Supamattaya  R Khongpradit  S Boonyaratpalin  M Kondo  K Kawai  R Kusuda  I Hirono  T Aoki 《Journal of fish diseases》1996,19(5):399-403

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The conservation and fishery benefits of protecting large pike (Esox lucius L.) by harvest regulations in recreational fishing     

Robert Arlinghaus  Shuichi Matsumura  Ulf Dieckmann 《Biological conservation》2010,143(6):1444-1459

Traditional fisheries management theory supports aggressive exploitation of old and large fish to maximize a stock’s biomass production and yield. Here we present an age-structured fish population model with multidimensional density-dependence to test the hypotheses that protection of large, fecund individuals is beneficial for the population and selected fisheries variables and that effects of maternal size on early survival rate change the resilience and fisheries productivity of a pike population (Esox lucius L.) exploited by recreational angling. We find that, compared to the traditional regulatory approach of management by small minimum-length limits (so that culling of large fish is encouraged), preservation of large and old individuals through harvestable-slot length limits promises considerable benefits for fisheries quality, without compromising the long-term conservation of the population. We also find that ignoring maternal effects on early survival of offspring might overestimate the equilibrium spawning stock abundance by up to 17% and the predicted harvest by up to 11%, potentially putting pike populations at risk from overharvest if size-dependent maternal effects are ignored in fisheries models. If the findings from our simulation study hold for empirical systems, they suggest altered harvest regulations in many of consumptive pike recreational fisheries are needed to protect large individuals to a greater extent that currently pursued.  相似文献   

Supplementation of Citric Acid and Amino Acid Chelated Trace Elements in Low‐Fish Meal Diet for Rainbow Trout Affect Growth and Phosphorus Utilization     

Adrián J. Hernández  Shuichi Satoh  Viswanath Kiron 《Journal of the World Aquaculture Society》2012,43(5):688-696

This study was conducted to evaluate the effect of citric acid (CA) and amino acid chelated trace elements (AA) on the growth, phosphorus (P) utilization efficiency and loading in rainbow trout fed a diet formulated with a low‐fish meal (FM) concentration. A low‐FM basal diet with or without monocalcium phosphate supplementation served as positive and negative control correspondingly. Dietary treatments consisted of the basal diet supplemented either with 1% CA, AA (equivalent to 40 Zn, 20 Mn, and 4 Cu mg/Kg of diet instead of inorganic trace element mix) or a combination of both. Duplicate groups of 35 fish (13.2 g) were fed until apparent satiation during 12 wk. Group of fish fed diets supplemented with 1% CA or AA had a final growth and feed utilization similar to that observed in fish fed the positive control diet (P > 0.05). P absorption was increased with the addition of CA or AA. P retention efficiency rates for these diets were higher in comparison with the rest of the treatments consequently showing the lowest P loading values (P < 0.05). The results indicate that addition of 1% CA or AA, to a low‐FM diet without inorganic P supplementation enhanced fish growth, P retention, and decreased P load to the environment.  相似文献   

Different combinations of protein ingredients in carp diets for reducing phosphorus loading     

Parveen  JAHAN  Takeshi  WATANABE  Shuichi  SATOH  Viswanath  KIRON 《Fisheries Science》2002,68(3):595-602

ABSTRACT: This study was conducted to gather data on the reduction of environmental phosphorus (P) loading through the formulation of carp diets with different combinations of protein ingredients. Five experimental diets were formulated by substituting fishmeal (FM; 10–20%) with alternative protein sources such as meat meal (MM; 5–15%), blood meal (BM; 5–7%), and defatted soybean meal (dSBM; 6–10%). The control diet used was a commercial carp diet selected based on earlier experiments. Each diet was fed to duplicate groups of juvenile carp three times a day, until satiation for 12 weeks. Feed performance was proportional to the increase in dietary FM levels. Phosphorus absorption ranged between 41.6% and 52.0% among the experimental groups and was 42.6% for the control group, but there were no marked differences in nitrogen (N) absorption rates. Phosphorus retention ranged from 31.4% to 35.7% for the test diets, whereas N retention increased proportionally with dietary FM levels and ranged from 34.7% to 41.7%. The P and N retention values of the control diet were 27.6% and 41.2%, respectively. The total P loading (T-P) increased at the higher FM levels (9.1–10.7 kg/t production), whereas lower FM levels produced higher total N loading (T-N, 34.6–43.1 kg/t production), the figures for the control being 13.9 T-P kg/t production and 35.6 T-N kg/t production. These results indicate that the reduction of FM levels to 10–20% by replacing it with MM, BM, and dSBM in carp diets was effective in reducing the loading of P and N.  相似文献   

Genetic organization of aromatic rice as revealed by RAPD markers: A case study in conserving crop genetic resources on farm   总被引：2，自引：0，他引：2  

Shuichi Fukuoka  Suu D Tran  Kaworu Ebana  Trinh N Luu  Tsukasa Nagamine  Kazutoshi Okuno 《Euphytica》2006,149(1-2):61-71

Summary On-farm conservation of landraces is one strategy to maintain the diversity of crop germplasm in local agro-ecosystems. The genetic structures of landraces are a key biological factor in on-farm conservation strategies. To accumulate a genetic understanding that will help establish a methodology for on-farm conservation, the genetic organization of landraces of aromatic rice in Namdinh province, Vietnam was analyzed using RAPD markers. Eighteen RAPD markers detected 38 genotypes among 320 aromatic rice samples growing at 23 sites of farmers' fields and in the experimental field that derived from 13 sites. Geographical variation was observed in the frequency of genotypes, whereas individual landraces could not be distinguished by RAPD markers. Genetic variation within a site was generally smaller than that among sites. The degree of genetic similarity of the plants in a site varied among sites, as did the number of genotypes. Changes in genetic structure over time were investigated using experimental populations each derived from approximately 30 plants from 13 farmers' fields. The differences detected by DNA markers between the genetic structural in the farmers' fields and those in experimental fields suggested that genetic drift is a major cause of these differences. The present study suggests that DNA markers are an essential means to monitor the genetic structures of heterogeneous landraces of rice, and are useful for selecting study sites for the on-farm conservation of genetic diversity as well as for successive monitoring.  相似文献   

In vivo antioxidative activity of propolis evaluated by the interaction with vitamins C and E and the level of lipid hydroperoxides in rats   总被引：4，自引：0，他引：4  

Sun F  Hayami S  Haruna S  Ogiri Y  Tanaka K  Yamada Y  Ikeda K  Yamada H  Sugimoto H  Kawai N  Kojo S 《Journal of agricultural and food chemistry》2000,48(5):1462-1465

In vivo antioxidative activity of propolis was evaluated on the basis of ameliorative effects on the oxidative stress induced by vitamin E deficiency in rats. The control group was fed vitamin E-deficient diet, and the propolis group was fed vitamin E-deficient diet supplemented with 1% of propolis for 4 and 8 weeks. Comparisons were made in tissue concentrations of vitamin C, vitamin E, and lipid hydroperoxides between these groups. No significant difference was observed in tissue vitamin E concentration between these groups after both 4 and 8 weeks. After 4 weeks, the plasma vitamin C concentration of the propolis group was significantly higher than that of the control group. After 8 weeks, the tissue concentrations of vitamin C in the kidney, stomach, small intestine, and large intestine of the propolis group were significantly higher than those of the control group. These results suggest that some components of propolis are absorbed to circulate in the blood and behave as a hydrophilic antioxidant that saves vitamin C. The concentration of lipid hydroperoxides in the large intestine of the propolis group was significantly lower than that of the control group after 8 weeks. These results suggest that propolis exerts its antioxidative effect where it is assumed to accumulate, such as on the kidney, where it is excreted, and on the gastrointestinal tract, where propolis influences these tissues even from the outside of the cell.  相似文献