Fostering sustainable water use in almond (Prunus dulcis Mill.) orchards in a semiarid Mediterranean environment     

Iván Francisco García-Tejero  Saray Gutiérrez Gordillo  Luciene Souza  Simón Cuadros-Tavira  Víctor Hugo Durán Zuazo 《Archives of Agronomy and Soil Science》2019,65(2):164-181

This work examines the long-term effects of deficit-irrigation (DI) practices in almond crop (Prunus dulcis Mill.) in agronomical and physiological terms. The trial was conducted during four-year monitoring period (2014–2017), in an experimental orchard (SW Spain), subjected to three irrigation regimes; i) a full-irrigation treatment (F_I), which received 100% of crop evapotranspiration (ET_C); ii) a regulated-deficit irrigation (RDI₅₀), which received 50% of ET_C during the kernel-filling period; and iii) a low-frequency deficit irrigation (LFDI), that was subjected to continuous periods of irrigation-restriction defined in terms of threshold values of leaf-water potential (Ψ_leaf) during the kernel-filling period. During the water stress period, there were monitored Ψ_leaf, stomatal conductance (g_s) and canopy temperature (T_C). Significant improvements in terms of water-use efficiency were found, as no differences in terms of yield between F_I and LFDI were found, leading to the conclusion that significant water savings (between 27 and 40%) can be achieved without compromising the yield. Moreover, threshold values of Ψ_leaf and thermal indicators were defined which will allow establishing future irrigation scheduling without compromising almond yield, especially when DI strategies are being applied.  相似文献   

Host range of Erwinia psidii and genetic resistance of Eucalyptus and Corymbia species to this pathogen     

Nilmara P. Caires  Pollyane S. Hermenegildo  Lúcio M. S. Guimares  Reginaldo G. Mafia  Edival . V. Zauza  Norton Borges Júnior  Jorge L. Badel  Acelino C. Alfenas 《Forest Pathology》2019,49(4)

Dieback caused by Erwinia psidii is currently one of the most important emerging diseases in eucalypt plantations in Brazil. However, little is known in terms of the host range of this pathogen or the potential sources of resistance against the disease it causes. In this study, we inoculated plants of species from nine families to gain insight into the host range of E. psidii. Plants of all inoculated species of Myrtaceae except Acca sellowiana exhibited disease symptoms and therefore represent potential hosts for the pathogen under natural conditions. In addition, the response of four Corymbia species, 29 Eucalyptus species and three interspecific Eucalyptus hybrids to inoculation with E. psidii was evaluated. All Corymbia henryi, Corymbia maculata, Eucalyptus thozetiana, Eucalyptus cloeziana, Eucalyptus viminalis, Eucalyptus dalrympleana and Eucalyptus pilularis plants were highly resistant to the pathogen, whereas differential disease resistance was observed in the other species. This study provides important information on sources of resistance to Erwinia psidii with potential use in the development of clones with enhanced resistance in eucalypt species of economic importance.  相似文献   

Long-term dynamics of leaf and root decomposition and nitrogen release in a grey alder (Alnus incana (L.) Moench) and silver birch (Betula pendula Roth.) stands     

Gunnar Morozov  Jürgen Aosaar  Mats Varik  Hardo Becker  Krista Lõhmus  Allar Padari 《Scandinavian Journal of Forest Research》2019,34(1):12-25

The decomposition of the leaf litter, fine roots (d?<?2?mm) and coarser roots (2?≤?d?<?5?mm) of grey alder and silver birch, as well as of α-cellulose sheets using the litterbag method was studied in two experimental stands on Podzoluvisol soils in Southern Estonia. For both tree species, the coarser roots decomposed faster than the fine roots, (p?<?.05), tree species did not affect the decomposition rate of the roots (p?>?.5). The nitrogen (N) input to soil from aboveground litter was multiple times higher than the N flux from roots. The remaining relative ash-free mass of the leaves of grey alder and silver birch after three and a half years was similar. After 11 years the remaining relative ash-free mass of the fine and coarser roots of grey alder still accounted for around 10% of the initial value. For silver birch the remaining value was around 20% after 9 years. The litterbag method to underestimates in fertile soils the decomposition of organic matter and thus did not reflect the actual dynamics of decomposition.  相似文献   

Atmospheric CO₂ enrichment and nutrient additions to planted soil increase mineralisation of soil organic matter, but do not alter microbial utilisation of plant- and soil C-sources   总被引：1，自引：0，他引：1  

Eric Paterson  Barry Thornton  Andrew J. Midwood  Shona M. Osborne  Allan Sim  Pete Millard 《Soil biology & biochemistry》2008,40(9):2434-2440

Plants link atmospheric and soil carbon pools through CO₂ fixation, carbon translocation, respiration and rhizodeposition. Within soil, microbial communities both mediate carbon-sequestration and return to the atmosphere through respiration. The balance of microbial use of plant-derived and soil organic matter (SOM) carbon sources and the influence of plant-derived inputs on microbial activity are key determinants of soil carbon-balance, but are difficult to quantify. In this study we applied continuous ¹³C-labelling to soil-grown Lolium perenne, imposing atmospheric CO₂ concentrations and nutrient additions as experimental treatments. The relative use of plant- and SOM-carbon by microbial communities was quantified by compound-specific ¹³C-analysis of phospholipid fatty acids (PLFAs). An isotopic mass-balance approach was applied to partition the substrate sources to soil respiration (i.e. plant- and SOM-derived), allowing direct quantification of SOM-mineralisation. Increased CO₂ concentration and nutrient amendment each increased plant growth and rhizodeposition, but did not greatly alter microbial substrate use in soil. However, the increased root growth and rhizosphere volume with elevated CO₂ and nutrient amendment resulted in increased rates of SOM-mineralisation per experimental unit. As rhizosphere microbial communities utilise both plant- and SOM C-sources, the results demonstrate that plant-induced priming of SOM-mineralisation can be driven by factors increasing plant growth. That the balance of microbial C-use was not affected on a specific basis may suggest that the treatments did not affect soil C-balance in this study.  相似文献   

Regression modeling nitrogen fertilization requirement for maize crop by combining spectral reflectance and agronomic efficiency     

Cláudio Kapp-Junior  Alaine Margarete Guimarães  André Carlos Auler 《Journal of plant nutrition》2020,43(14):2152-2163

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Genetic diversity and aggressiveness of Calonectria pteridis in Eucalyptus spp.     

R. G. Freitas  R. F. Alfenas  L. M. S. Guimarães  J. L. Badel  A. C. Alfenas 《Plant pathology》2019,68(5):869-877

Calonectria leaf blight, caused by Calonectria pteridis, is currently one of the main foliar diseases in eucalypt plantations in Brazil. In warm and high rainfall regions, the disease can be a limiting factor for eucalypt production when planting susceptible genotypes. The most effective method for controlling this disease in the field is the use of resistant genotypes, which requires knowledge of the genetic variability and aggressiveness of the pathogen population for effective deployment of plant resistance. This work evaluated the genetic diversity and aggressiveness of C. pteridis populations obtained from infected eucalypt plants in Monte Dourado (Pará state) and Imperatriz (Maranhão state), Brazil. To study the genetic diversity, 16 ISSR primers were tested, five of which amplified polymorphic, reproducible and informative bands. Thirty-one closely related genotypes were identified from 84 isolates studied, indicating that the population has a low genetic diversity. The aggressiveness of seven isolates, selected according to geographic origin and their clustering in the ISSR-based dendogram, was determined by inoculation of a hybrid Eucalyptus grandis × E. urophylla clone under controlled conditions. Disease severity was assessed by both measuring the percentage of plant defoliation and assigning a score according to a diagrammatic scale of symptoms. A high correlation between the two evaluation methods was observed, which revealed significant differences in aggressiveness among the isolates. The diagrammatic scale is recommended for disease evaluation because results are obtained much faster, before the occurrence of severe defoliation. No correlation between clustering in the ISSR-based phylogenetic analysis and aggressiveness was observed.  相似文献   

Movement patterns of an avian generalist predator indicate functional heterogeneity in agricultural landscape     

Väli  Ülo  Mirski  Paweł  Sein  Gunnar  Abel  Urmas  Tõnisalu  Grete  Sellis  Urmas 《Landscape Ecology》2020,35(7):1667-1681

Landscape Ecology - Biodiversity is closely related to landscape heterogeneity, but both are difficult to evaluate directly. Apex predators, such as raptors, are potential surrogate indicators of...  相似文献   

Effects of exercise on the morphology of the myenteric neurons of the duodenum of Wistar rats during the ageing process     

De Britto Mari R  Clebis NK  Gagliardo KM  Guimarães JP  Stabille SR  De Mello Germano R  De Souza RR 《Anatomia, histologia, embryologia》2008,37(4):289-295

This study aimed to evaluate the effects of regular physical activity on the morphology of the myenteric plexus of the duodenum in rats during the ageing process. To this end, 45 Wistar rats were divided into three groups: C (sedentary – 6 months old), S (sedentary – 12 months old) and T (trained – 12 months old). The animals of group S were given with a physical activity programme consisting of a 10-min-treadmill workout once a week. The animals of group T were submitted to the physical activity programme five times a week. Their duodenums were collected and submitted to the techniques of nicotinamide adenine dinucleotide (NADH)-diaphorase enzyme histochemistry for whole-mount preparations and transmission electron microscopy. No differences in the constitution of the myenteric plexuses were found when the sedentary and trained groups were compared with the control group. The ultrastructural features were similar for the three groups. However, it was verified that the physical activity of the trained animals resulted in a similar myenteric neuron morphology to that of the adult animals (6 months old), thereby confirming its beneficial effect, as the sedentary animals had larger alterations in the collagen fibrils and the basal membrane that occur through ageing. The quantitative analysis showed that the NADH-diaphorase positive neurons decreased with ageing and increased with physical activity ( P  > 0.05). No significant alteration ( P  > 0.05) in the neuronal profile area of the NADH-diaphorase positive neurons has been observed with ageing.  相似文献   

Bovine Oocyte Quality in Relation to Ultrastructural Characteristics of Zona Pellucida,Polyspermic Penetration and Developmental Competence     

P Santos  A Chaveiro  N Simões  F Moreira da Silva 《Reproduction in domestic animals》2008,43(6):685-689

In the present study, the effect of bovine oocyte quality related to ultrastructural characteristics of zona pellucida (ZP), polyspermic penetration and embryo developmental competence was evaluated. Cumulus–oocyte complexes were punctioned from 453 ovaries, classified as 1, 2, 3 and 4 according to their morphological aspect, matured for 24 h and then divided into two groups. In group A, oocytes were fixed in 2.5% glutaraldehyde and 0.1 m sodium cacodylate and examined under a scanning electron microscope. Photomicrographs were taken and ZP’s pores were evaluated in squares of 6.4‐μm width. In group B, oocytes were fertilized in vitro. After 48 h, non‐cleaved oocytes were fixed for polyspermy evaluation. On days 7, 9 and 10, embryos were classified as developed (blastocysts and hatched blastocysts). Results showed that quality 1 oocytes revealed a ZP pore diameter of 0.50 ± 0.07 μm, which was smaller than the observed on oocytes of quality 2 (0.83 ± 0.10 μm), quality 3 (1.02 ± 0.22 μm) and quality 4 (1.38 ± 0.59 μm) (p ≤ 0.05). For In Vitro Fertilization (IVF), results showed that embryos originating from oocytes classed as 3 and 4 had lower cleavage rate (68.4% and 43.8%) than those belonging to class 1 and 2 (79.5% and 69.3%) (p ≤ 0.05). None oocyte classified as 3 and 4 developed to hatch blastocysts, while for oocytes belonging to quality 1 and 2, these values were, respectively, 15.2% and 12.5%. Concerning polyspermy, oocytes class 1 and 2 had lower polyspermic penetration than those belonging to class 3 and 4 (respectively 4.1%, 4.5%, 11.1% and 9.8%, for class 1, 2, 3 and 4). In conclusion, the present study demonstrated that oocytes with low qualities result in lower developmental competence and with high percentage of polyspermy after IVF, which can be the result of the ZP structure such as the number and the pore’s diameter.  相似文献   

Milk production and composition,food consumption,and energy balance of postpartum crossbred Holstein-Gir dairy cows fed two diets of different energy levels     

dos Santos  Gustavo Bervian  Brandão  Felipe Zandonadi  dos Santos Ribeiro  Lilian  Ferreira  Alexandre Lima  Campos  Mariana Magalhães  Machado  Fernanda Samarini  de Carvalho  Bruno Campos 《Tropical animal health and production》2019,51(1):65-71

The objective of this study was to evaluate the production, consumption, and energy balance parameters of primiparous 3/4 and 7/8 Holstein × Gir (HG) dairy cows fed two diets of differing energy levels during the postpartum period. At the beginning of the study, 28 days prepartum, the average weight of both genetic groups was 498?±?12 kg and body condition score (BCS) was 3.5?±?0.05. At the end of the study, 61 days postpartum, the 3/4 HG cows had higher weight and body condition scores than the 7/8 HG (456?±?8 and 429?±?8 kg and 3.13?±?0.03 and 2.94?±?0.03 BCS for 3/4 HG and 7/8 HG, respectively). Milk from cows fed the high-energy diet had higher percentages of fat, protein, lactose, and total dry extract than cows fed the low-energy diet. Cows fed the high-energy diet had higher net energy intake (95.3?±?1.9 vs. 88.1?±?2.1 MJ/day) and higher energy balance (3.64?±?2.13 vs ??6.02?±?2.30 MJ/day). The 3/4 HG cows displayed higher energy for maintenance (33.1?±?0.4 MJ/day) than the 7/8 HG (31.5?±?0.5 MJ /day). In conclusion, although the primiparous 3/4 HG were heavier than the 7/8 HG and had a higher body condition score, no differences in milk produced up to 60 days postpartum were observed. The higher energy diet during the postpartum period increased energy balance, resulting in higher production of milk fat, protein, and lactose.

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