猪细小病毒的PCR检测、分离及鉴定     

刘金彪  姚月琴  高崧  彭大新  刘秀梵 《中国兽药杂志》2008,42(12)

根据GenBank中报道的猪细小病毒基因组序列,利用PCR引物设计软件,设计并合成了1对引物,通过对PCR反应条件的优化,成功地扩增出了511bp的目的片段,建立了PCR检测PPV的方法。利用该方法对120份临床样品进行检测,共检测到4份阳性样品,并成功地分离到2株PPV,1#病毒分离株理化特性鉴定表明其符合PPV的特性。  相似文献   

Vaccination of chickens with a chimeric DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 induces protective immunity against coccidiosis   总被引：2，自引：0，他引：2  

Xu Q  Song X  Xu L  Yan R  Shah MA  Li X 《Veterinary parasitology》2008,156(3-4):319-323

A fusion DNA vaccine co-expressed Eimeria tenella TA4 and chicken IL-2 (chIL-2) was constructed and its efficacy against E. tenella challenge was observed. TA4 gene of E. tenella and chIL-2 gene were cloned into expression vector pcDNA3.1 and pcDNA4.0c in different forms, producing vaccines pcDNA3.1-TA4-IL-2, pcDNA3.1-TA4 and pcDNA4.0c-IL-2. The expression of aim genes in vivo was detected by RT-PCR and western blot. Animal experiment was carried out to evaluate the immune efficacy of the vaccines. Results indicated these DNA vaccines were successfully constructed and the antigen genes could be expressed effectively in vivo. The animal experimental results showed that DNA vaccines could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease ratio. The ACI of pcDNA3.0-TA4-IL-2 group was 192, higher than that of pcDNA3.1-TA4 group. The results suggested that TA4 was an effective candidate antigen for vaccine and co-expression of cytokine with antigen was an alternative method to enhance DNA vaccine immunity.  相似文献   

无血清培养基IVD101和G1/G2在牛体细胞核移植中的应用研究     

李荣  刘颖  赵兴波  王莉莉  王海萍  丁方荣  李京  李松  高凤磊  戴蕴平 《畜牧兽医学报》2008,39(11)

通过考察牛体细胞核移植重构胚在无血清培养基（IVD101、G1／G2）条件下培养的囊胚发育率及其质量,从而评估无血清培养基支持牛体细胞核移植重构胚体外发育的能力。采用IVD101和G1／G2对牛体细胞核移植胚胎进行体外培养,并以CR1aa＋5％FBS作为对照组。无血清培养基IVD101和G1／G2的囊胚发育率与对照组无显著性差异（41．2％±9．1％、42．2％±10．8％,48．0％±9．2％,P〉0．05）。通过囊胚差异染色和冷冻／解冻胚胎存活率分析胚胎质量,发现无血清培养基ICM／Total略低于对照组（31．8％±10．5％、29．5％±11．9％vs ．33．0％±14．8％）,但无显著性差异（P〉0．05）;3个组别的囊胚经程序化冷冻／解冻后无血清培养基的存活率（IVD101、G1／G2）略高于对照组,但差异不显著（84．8％、80．4％＇US．77．3％,P〉0．05）。结果证明无血清培养基（IVD101、G1／G2）可以支持体细胞核移植重构胚的体外发育,且其对程序化冷冻的耐受性与添加血清组（CR1aa＋5％FBS）相似。  相似文献   

Characterization of integrons-mediated antimicrobial resistance among Escherichia coli strains isolated from bovine mastitis     

Wang GQ  Wu CM  Du XD  Shen ZQ  Song LH  Chen X  Shen JZ 《Veterinary microbiology》2008,127(1-2):73-78

To assess the prevalence of antimicrobial resistance and class I integrons in Escherichia coli strains (n=58) isolated from bovine mastitis in Inner Mongolia, antimicrobial susceptibility and the presence of various types of integrons were characterized. Most isolates were susceptible to amikacin, colistin, ceftazidime, gentamicin and kanamycin, while those also exhibited high resistant incidence rates to ampicillin, amoxicillin, sulfadiazine and sulfamethoxydiazine. The integrase gene of integrons was amplified by PCR using degenerate primers. The integrons were confirmed by restriction fragment length polymorphism (RFLP) analysis of positive PCR products. Neither class II nor class III integron was detected, while 56.90% (n=33) of the isolates were positive for the presence of intI1 gene. Sequencing analysis of gene cassettes revealed that seven gene cassettes were found, which encoded resistance to trimethoprim (dfrA1 and dfrA17), aminoglycosides (aacA4, aadA1 and aadA5) and chloramphenicol (catB3), respectively. Of them, the gene cassette array dfrA17-aadA5 was found most prevalent (62.96%). The percentage of positive-integron among the isolates whose resistant profile was relatively broad (n> or =7) is 100.00%, while the one in narrow-profile isolates (n=2-6) is 30.56%. The correlation analysis revealed the incidence of integrons among the isolates were highly related to the resistant profile, indicating integrons play an important role in the dissemination and spread of the antimicrobial resistant strains.  相似文献   

Long-term survival of dogs after cholecystoenterostomy: a retrospective study of 15 cases (1981-2005)     

Papazoglou LG  Mann FA  Wagner-Mann C  Song KJ 《Journal of the American Animal Hospital Association》2008,44(2):67-74

Fifteen dogs with extrahepatic biliary tract disease underwent cholecystoenterostomy. Long-term survivors were significantly older at presentation (mean age 140.5 months) than dogs that survived the first 20 days after surgery but subsequently died from causes related to the surgery or hepatobiliary disease (mean age 72 months). Dogs that died during the first 20 days had significantly more complications in the hospital than dogs that survived this period. The type of underlying hepatobiliary disease (i.e., benign or malignant) was not associated with either short-term outcome or long-term survival. Eight dogs died from causes related to surgery or hepatobiliary disease. Long-term complications included hepatic abscesses, acquired portosystemic shunts, pancreatitis, and vomiting.  相似文献   

Corticosterone administration and dietary glucose supplementation enhance fat accumulation in broiler chickens   总被引：1，自引：0，他引：1  

Jiang KJ  Jiao HC  Song ZG  Yuan L  Zhao JP  Lin H 《British poultry science》2008,49(5):625-631

1. The effects of exogenous corticosterone administration and glucose supplementation on energy intake, lipid metabolism and fat deposition of broiler chickens were investigated. 2. A total of 144 three-d-old male chickens were randomly assigned to one of the following 4 treatments for 7 d: a low energy diet (10.9 MJ ME/kg, 200 g/kg CP) with or without corticosterone (30 mg/kg diet) and drinking water supplemented with glucose (80 g/l) or saccharine (2 g/l, control). 3. Body weight (BW) gain and breast and thigh muscle yields (% body mass) were all significantly decreased by corticosterone treatment. The relative cumulative feed intake (RCFI) and relative ME intake (RMEI), rather than the feed (FI) or ME intake (MEI) were increased by corticosterone administration. Both feed efficiency (FE) and caloric efficiency (CE) were decreased by corticosterone administration. Corticosterone administration had no obvious effect on water consumption. 4. Glucose supplementation had no influence on BW gain and breast and thigh muscle yield (as % of body mass). FI or RCFI was decreased while MEI or RMEI was increased by glucose supplementation. FE was improved by glucose treatment, whereas CE was reduced. 5. Liver weight and abdominal, cervical and thigh fat deposits were all significantly increased by either corticosterone or glucose treatment. 6. Plasma concentrations of glucose, urate, triglyceride, non-esterified fatty acids (NEFA), very low density lipoprotein and insulin were all significantly increased by corticosterone treatment. Glucose supplementation had no obvious influence on any of the measured plasma parameters except for NEFA, which were significantly increased. 7. Lipoprotein lipase activities in either cervical or abdominal adipose tissues, rather than in thigh fat tissue, were significantly elevated by either glucose or corticosterone treatment.  相似文献   

我国野生白头鹤研究现状及保护对策初探   总被引：2，自引：0，他引：2  

张佰莲  宋国贤 《野生动物》2008,29(4)

针对我国野生白头鹤种群濒危现状,我们搜集近15 a关于白头鹤的科学研究文献与报道,并将其按种群数量与分布和野外生态学进行归类.分析表明,目前我国白头鹤研究主要集中在种群数量分布调查与越冬生态的研究方面:此外还涉及到食性研究及迁徙等报道;关于白头鹤繁殖生态的生物学信息比较匮乏,尤其是繁殖期巢址生境的选择未见报道,有待于开展深入的研究.  相似文献   

雪灾对中国南方自然保护区生态环境破坏的调查研究     

宋慧刚 《野生动物》2008,29(3):147-149

2008年历史罕见的低温雨雪冰冻气候,使中国南方大部分省份的自然保护区和保护区内的野生动植物资源及基础设施遭受重创,本文作者对灾情进行了调查研究,并提出了重建建议.  相似文献   

生态公路建设中野生动物保护意义与对策   总被引：1，自引：0，他引：1  

马武昌  宋子炜 《野生动物》2008,29(6)

随着公路建设的飞速发展,生态公路作为公路建设新理念受到了相关部门的高度重视,本文在总结国内外生态公路研究和实际工作经验的基础上,分析了生态公路内涵,并指出野生动物保护在生态公路建设中的意义以及保护对策,这对今后的生态公路建设具有一定的借鉴意义。  相似文献   

Seroprevalence of leptospirosis in animals in Korea     

Jung BY  Song YK  Choi JS  Lee KW  Ha TY  Yoon SS  Kweon CH  Park CK 《The Veterinary record》2008,163(1):28-29

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