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41.
42.
Sylvie L Benestad Lars Austb? Michael A Tranulis Arild Espenes Ingrid Olsaker 《Veterinary research》2012,43(1):87
Prion diseases such as scrapie in small ruminants, bovine spongiform encephalopathy (BSE) in cattle and Creutzfeldt-Jakob disease (CJD) in man, are fatal neurodegenerative disorders. These diseases result from the accumulation of misfolded conformers of the host-encoded prion protein (PrP) in the central nervous system. To date naturally-occurring PrP free animals have not been reported. Here we describe healthy non-transgenic animals, Norwegian Dairy Goats, lacking prion protein due to a nonsense mutation early in the gene. These animals are predicted to be resistant to prion disease and will be valuable for research and for production of prion-free products. 相似文献
43.
Olivier Taugourdeau Jean Dauzat Sébastien Griffon Sylvie Sabatier Yves Caraglio Daniel Barthélémy 《Annals of Forest Science》2012,69(6):713-721
Context
Understanding the effects of exogenous factors on tree development is of major importance in the current context of global change. Assessing the structure development of trees is difficult given that they are large and complex organisms with lifespan of several decades.Aims
We used a retrospective analysis to derive the ontogenetic trends in silver fir development and assess the effects of climate or light environment on tree architecture.Methods
Thanks to the identification of relevant growth markers (bud cataphylls and pseudo-whorl branches), a retrospective analysis allowed to record annual shoot extension and to date them on silver firs of various sizes under different environmental conditions.Results
The length of successive annual shoots located on different axes clearly show gradual trends related to the physiological age of meristems. Within- and between-tree variations are noted due to the plasticity of development and growth induced by light environment and climate.Conclusion
Retrospective analysis is an efficient method for getting information on the history of trees architecture and subsequently to relate it to environmental factors. 相似文献44.
Geert Paes Dominique Paepe Evelyne Meyer Annemarie T Kristensen Luc Duchateau Miguel Campos Sylvie Daminet 《Acta veterinaria Scandinavica》2013,55(1):74
Background
Diagnosing canine immune-mediated haemolytic anaemia (IMHA) is often challenging because all currently available tests have their limitations. Dogs with IMHA often have an increased erythrocyte osmotic fragility (OF), a characteristic that is sometimes used in the diagnosis of IMHA. Since the classic osmotic fragility test (COFT) is time-consuming and requires specialized equipment, an easy and less labour-intensive rapid osmotic fragility test (ROFT) has been used in some countries, but its diagnostic value has not yet been investigated.This study aimed to evaluate erythrocyte osmotic fragility in dogs with and without IMHA, to compare results of the classic (COFT) and rapid (ROFT) test and to assess the value of the ROFT as diagnostic test for canine IMHA.Nineteen dogs with IMHA (group 1a), 21 anaemic dogs without IMHA (group 1b), 8 dogs with microcytosis (group 2), 13 hyperlipemic dogs (group 3), 10 dogs with lymphoma (group 4), 8 dogs with an infection (group 5) and 13 healthy dogs (group 6) were included.In all dogs, blood smear examination, in-saline auto-agglutination test, Coombs’ test, COFT and ROFT were performed. In the COFT, OF5, OF50 and OF90 were defined as the NaCl concentrations at which respectively 5, 50 and 90% of erythrocytes were haemolysed.Results
Compared with healthy dogs, OF5 and OF50 were significantly higher in group 1a (P < 0.001) and OF5 was significantly higher in group 3 (P = 0.0266). The ROFT was positive in 17 dogs with IMHA, 10 hyperlipemic dogs, one anaemic dog without IMHA and one healthy dog.Conclusions
Osmotic fragility was increased in the majority of dogs with IMHA and in dogs with hyperlipidemia, but not in dogs with microcytosis, lymphoma or an infection. Although more detailed information was obtained about the osmotic fragility by using the COFT, the COFT and ROFT gave similar results. The ROFT does not require specialized equipment, is rapid and easy to perform and can be used easily in daily practice. Although, the ROFT cannot replace other diagnostic tests, it may be a valuable additional tool to diagnose canine IMHA. 相似文献45.
Assessing the effects of the clumping phenomenon on BRDF of a maize crop based on 3D numerical scenes using DART model 总被引:2,自引:0,他引:2
Sylvie Duthoit Valrie Demarez Jean-Philippe Gastellu-Etchegorry Emmanuel Martin Jean-Louis Roujean 《Agricultural and Forest Meteorology》2008,148(8-9):1341-1352
Inverting radiative transfer (R-T) models against remote sensing observations to retrieve key biogeophysical parameters such as leaf area index (LAI) is a common approach. Even if new inversion techniques allow the use of three-dimensional (3D) models for that purpose, one-dimensional (1D) models are still widely used because of their ease of implementation and computational efficiency. Nevertheless, they assume a random distribution of foliage elements whereas most canopies show a clumped organization. Due to that crude simplification in the representation of the canopy structure, sizeable discrepancies can occur between 1D simulations and real canopy reflectance, which may further lead to false LAI values. The present investigation aims to appraise to which extent the incorporation of a clumping index (noted λ) into 1D R-T model could improve the simulations of Bidirectional Reflectance Distribution Function (BRDF). Canopy BRDF is simulated here for three growth stages of a maize crop with the Discrete Anisotropic Radiative Transfer (DART) model in the visible and near infrared spectral bands, for two contrasted soil types (dark and bright) and different levels of heterogeneity to represent the canopy structure. 3D numerical scenes are based on in-situ structural measurements and associated BRDF simulations are thus considered as references. 1D scenarios assume either that leaves are randomly distributed (λ = 1) or clumped (λ < 1). If BRDF simulations seem globally reliable under the assumption of a random distribution in near infrared, it can also lead to relative errors on the total BRDF up to 30% in the red spectral band. It comes out that the use of a clumping index in a 1D reflectance model generally improves BRDF simulations in the red considering a bright soil, which seems relatively independent of LAI. In the near infrared, best results are usually obtained with homogeneous canopies, except with the dark soil. Clearly, influent factors are mainly the LAI and the spectral contrast between soil and leaves. 相似文献
46.
Helge Sierotzki Sandro Parisi Ute Steinfeld Isabel Tenzer Sylvie Poirey Ulrich Gisi 《Pest management science》2000,56(10):833-841
Field isolates of Mycosphaerella fijiensis, causing black Sigatoka of banana, were characterised for their sensitivity to different inhibitors of the cytochrome bc1 enzyme complex (Qo respiration inhibitors, strobilurin fungicides), using physiological, biochemical and molecular genetic methods. Strobilurin‐resistant isolates exhibited very high resistance factors both in mycelial growth inhibition and NADH consumption assays. Cross‐resistance was observed among all Qo inhibitors, including trifloxystrobin, azoxystrobin, famoxadone, strobilurin B and myxothiazol. However, the Qi and the cytochrome aa3 inhibitors, antimycin A and potassium cyanide, respectively, were not cross‐resistant to Qo inhibitors. In sensitive but not in resistant isolates, mixtures of Qo inhibitors and SHAM, an inhibitor of the alternative oxidase (AOX), were more active than the components alone, indicating that the alternative pathway is essential in metabolism, but not causal for resistance. In the cell‐free NADH‐consumption assay, the Qo inhibitors affected the sensitive but not the resistant isolates, suggesting that AOX was not active in sub‐mitochondrial particles. In whole cells, however, the AOX has a basic expression level and is probably not inducible by trifloxystrobin. Sequencing of the cytochrome b gene of sensitive and resistant M fijiensis isolates revealed a difference in the nucleotide sequence leading to a single amino acid change from glycine to alanine at position 143 in the resistant isolate. This change is known to occur also in the naturally tolerant basidiomycete Mycena galopoda. It is suggested that the field isolates of M fijiensis can acquire resistance to Qo inhibitors due to a target site alteration with a single base pair change. Resistant isolates do not seem to contain a mixture of mutated and non‐mutated DNA, indicating a complete selection of resistant mitochondria and a maternally donated mode of resistance. © 2000 Society of Chemical Industry 相似文献
47.
Susceptibility in field populations of codling moth,Cydia pomonella (L.) (Lepidoptera: Tortricidae), in Ontario and Quebec apple orchards to a selection of insecticides 下载免费PDF全文
48.
Condensed tannins (CT) can strongly affect litter decomposition, but their fate during the decomposition process, in particular as influenced by detritivore consumption, is not well understood. We tested the hypothesis that litter CT are reduced by the gut passage of two functionally distinct detritivores of Mediterranean forests, the millipede Glomeris marginata, and the land snail Pomatias elegans, as a fixed proportion of initial litter CT, but more so in Pomatias since snails are known to have a more efficient enzymatic capacity. Contrary to our hypothesis, both detritivore species reduced litter CT to near zero in their faecal pellets irrespective of the wide range in initial leaf litter CT concentrations of 9-188 mg g−1 d m among three Mediterranean tree species (Pistacia terebinthus, Quercus ilex, Alnus glutinosa) and different decomposition stages of their litter. The almost complete disappearance of CT even from some litter types highly concentrated in CT, due to either degradation by gut microorganism or complexation of CT into insoluble high molecular weight structures, suggests a high “de-tanning” efficiency across functionally distinct detritivore species. The transformation of CT-rich litter into virtually CT-free faecal pellets by detritivores might be highly relevant for the subsequent decomposition process in ecosystems with a high macrofauna abundance and CT-rich plant species such as Mediterranean forests. 相似文献
49.
Céline Leroy Sylvie Sabatier Novi Sari Wahyuni Jean-François Barczi Jean Dauzat Marilyne Laurans Daniel Auclair 《Agroforestry Systems》2009,77(1):37-47
In agroforestry systems, the distribution of light transmitted under tree canopies can be a limiting factor for the development
of intercrops. The light available for intercrops depends on the quantity of light intercepted by tree canopies and, consequently,
on the architecture of the tree species present. The influence of tree architecture on light transmission was analysed using
dynamic 3D architectural models. The architectural analysis of Acacia mangium and Tectona grandis was performed in Indonesian agroforestry systems with trees aged from 1 to 3 years. 3D virtual trees were then generated
with the AmapSim simulation software and 3D virtual experiments in which tree age, planting density, planting pattern and
pruning intensity varied were reconstructed in order to simulate light available for the crop. Canopy closure of trees was
more rapid in A. mangium than in T. grandis agroforestry systems; after 3 years the quantity of light available for A. mangium intercrops was three times lower than under T. grandis. Simulations with A. mangium showed that practices such as pruning and widening tree spacing enable to increase the total transmitted light within the
stand. On T. grandis, modification of the tree row azimuth resulted in changes in the spatial and seasonal distribution of light available for
the intercrops. These results are discussed in terms of agroforestry system management.
相似文献
Sylvie SabatierEmail: |
50.
Annamaria Leccese Sylvie Bureau Maryse Reich M. G. C. Catherine Renard Jean-Marc Audergon Carmelo Mennone Susanna Bartolini Raffaella Viti 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(2):112-120
We have investigated the effect of cultivation systems and fruit post-harvest management on the antioxidant properties of
apricot fruits. Trees of five cultivars ‘Tyrinthos’, ‘Cafona’, ‘Bella d’Italia’, ‘Vitillo’ and ‘Pellecchiella’ were cultivated
under integrated and organic systems. Fruits were collected at full maturity stage and analyzed either immediately or after
storage at 4±0.5 °C and 85% of relative humidity for seven and 14 days. The main pomological traits (weight, colour, flesh
firmness, total soluble sugars, titratable acidity) and antioxidant properties were analyzed. The total antioxidant capacity
(TAC by TEAC method), total phenols content (TP by Folin-Ciocalteu method) and carotenoid content by HPLC-DAD were monitored.
Cultivar characterization by principal component analysis (PCA) indicated a large variability on pomological and antioxidant
properties of apricot fruits. ‘Bella d’Italia’ showed better TAC and TP values compared to the other cultivars. ANOVA interactions
between cultivar and cultivation system (organic/integrated) were found for the antioxidant properties. These interactions
may help to select a set of genotypes with better performances under organic system, which in our study might be indicated
in ‘Cafona’ and ‘Bella d’Italia’. 相似文献