Influence of ischemia-reperfusion on apoptosis of sinoatrial node cells in rabbits in vivo     

SONG Zhi-yuan  LI Yong-hua  YAO Qing  TONG Shi-fei 《园艺学报》2003,19(12):1606-1609

AIM:To study influence of ischemia-reperfusion(IR) on apoptosis and expression of apoptosis-related genes Fas-L, Bax and Bcl-2 of sinoatrial node(SAN) cells in rabbits in vivo. METHODS:Ninety healthy adult rabbits were divided randomly into control group, ischemia groups (I_{10 min}, I_{30 min}, I_{60 min} and I_{120 min}) and IR groups (I_{10 min}R_4h, I_{30 min}R_4h, I_{60 min}R_4hand I_{120 min}R_4h). IR injury model of SAN was established by occluding and loosening the start section of right coronary artery. The apoptosis of SAN cells was detected by TUNEL staining. The expression of Fas-L, Bax and Bcl-2 of SAN cells was detected by immunohistochemistry. RESULTS:①No obvious apoptosis of SAN cells was observed in control group, I_{10 min} and I_{30 min} groups. Apoptosis of different degrees in SAN cells were found in 68.3%(41/60) rabbits in I_{60 min}, I_{120 min} and 4 subgroups of IR. ②The highest expression of Fas-L and Bax was observed in I_{120 min} group and that of Bcl-2 was in I_{60 min} group. ③The highest expression of Fas-L and Bax was observed in I_{60 min}R_4h group. The peak level of Bcl-2 was observed in I_{30 min}R_4h group. ④The expression of Fas-L and Bax was significant higher in IR group than that in ischemic group at the same time point. CONCLUSION:Ischemia and IR induced apoptosis of SAN cells in rabbit in vivo. Fas-L、Bax、Bcl-2 may participate in the regulation of apoptosis and the injury during IR aggravates the apoptosis of SAN cells.  相似文献   

Establishment of chronic obstructive pulmonary disease rat models by passive cigarette smoking and intratracheal instillation of lipopolysaccharide     

LI Hong-mei  CUI De-jian  TONG Xin  PANG Bao-sen  GAO Ya-bing  WANG De-wen 《园艺学报》2002,18(7):808-812

AIM:To establish rat chronic obstructive pulmonary disease(COPD) models by passive cigarette smoking plus intratracheal instillation of lipopolysacchride(LPS) or passive cigarette smoking only, which would be similar to the pathogenesis of human COPD. METHODS:48 Wistar rats were randomly divided into 4 groups.(1) Healthy control I group(n=12), rats were bred 4 weeks;healthy control II group(n=12), rats were bred for 3months. (2) Model group I (n=12), 200μg lipopolysaccharide(LPS) was instilled intratracheally once for every two weeks and the rats were exposured to 5% of cigarette smoke, 0.5 h/d for 4 weeks.(3) Model group II(n=12),rats were exposed to 5% of cigarette smoke, 0.5 h/d for 3 months. The pathologic changes of airways and lung tissues, pulmonary function and blood gas analysis were determined. The airway wall lymphocytes and alveolar macrophages were counted. The cross areas of epithelial layer, smooth muscle layer and lamina propria of bronchi were measured. The hydroxyproline of lung tissue homogenates was determined by biochemistry method.RESULTS:The pathologic changes of airways and lung tissue of two models were similar to but milder than those of COPD patients(biopsy data). The collagen deposition and the cross areas of epithelial layer and smooth muscle layer in airway walls of two model groups were significantly increased than those of control groups(P＜0.01,P＜0.05).FEV_0.3/FVC% of two model groups, PaO₂ and SaO₂ of model I group were significantly decreased, while R_i and R_e in model I group were significantly increased than that of control I group(P＜0.05). The PaCO₂ and the counts of lymphocytes and alveolar macrophages of both model groups were significantly increased than those of the control groups (P＜0.01). Lots of alveolar macrophages had phagocyted smoke granules. The amounts of hydroxyproline of two model groups were significantly increased than those of control group((P＜0.05) and were negatively related to the FEV_0.3/FVC%, respectively (P＜0.01,P＜0.01) and positively related to airway resistance of model I group(P＜0.01). CONCLUSIONS:COPD rat models were successfully established by passive cigarette smoking plus intratracheal instillation of LPS and cigarette smoking only. The pathologic changes were similar but milder than those of COPD patients. The airway obstruction of model I group was more severe than that of model II group, but they have no significant difference.  相似文献   

(土娄)土区不同蔬菜对钾肥的肥效反应     

赵护兵  同延安  赵尊练  司立征  高义民 《干旱地区农业研究》2004,22(1):138-141

在陕西省关中土娄土区布置线辣椒、甘蓝、白菜、芹菜钾肥大田试验,研究不同蔬菜对钾肥的肥效反应。结果表明,增施钾肥可使蔬菜硝态氮含量降低11.8%～116.0%,维生素C含量增加0.4～147.4mg/kg;产量增加9.3%～33.8%;明显促进营养生长和生殖生长;400cm深土壤剖面硝态氮累积量减少22.7～48.0kg/hm2,尤其在80～220cm剖面减少最为明显。  相似文献   

抗鸡传染性喉气管炎重组鸡痘病毒基因工程疫苗同居感染试验   总被引：3，自引：0，他引：3  

王云峰  智海东  王玫  孙永科  仇华吉  周艳君  田志军  张绍杰  童光志 《中国预防兽医学报》2004,26(1):51-54

疫苗的接触传播是疫苗免疫接种需要考虑的重要因素,为了检测重组鸡痘病毒载体疫苗水平传播的能力,对隔离条件下饲养的SPF鸡用重组鸡痘病毒基因工程疫苗接种,同时设立非免疫对照鸡,饲养期间特意延长清粪时间以增加感染的机会,1个月之后攻击传染性喉气管炎WG株强毒和鸡痘102株强毒,疫苗免疫鸡全部获得保护,而非免疫鸡则全部发病.在试验动物饲养场的自然条件下,将免疫鸡和试验对照两组鸡饲养在同一个鸡舍内,让疫苗毒的传播更接近自然条件.在每个月的攻毒试验中,对照鸡都没有获得对鸡痘和传染性喉气管炎强毒的保护.在疫苗免疫期间进行连续5个月的跟踪检测,同居未免疫鸡没有检测到抗传染性喉气管炎病毒gB抗体.这些实验结果表明抗鸡传染性喉气管炎重组鸡痘病毒基因工程疫苗不能通过接触传播.  相似文献   

鸡传染性支气管炎病毒S1基因和鸡Ⅱ型干扰素基因在重组鸡痘病毒中的共表达   总被引：5，自引：3，他引：5  

孙永科  田占成  王云峰  童光志  智海东  刘胜旺  王玫  杨增岐 《畜牧兽医学报》2005,36(8):800-806

将鸡Ⅱ型干扰素（ChIFNγ）基因和鸡传染性支气管炎病毒S1基因同时插人到鸡痘病毒转移载体中，构建含有这2个基因的鸡痘病毒转移载体pSY—ChIFNγS1。采用脂质体法将该质粒转染鸡痘病毒感染的鸡胚成纤维细胞（CEF），经过8轮蓝斑筛选，得到纯化的能够同时表达鸡Ⅱ型干扰素和鸡传染性支气管炎病毒S1基因的重组鸡痘病毒rFPV—ChIFNγS1。rFPV—ChIFNγS1接种28日龄的SPF鸡1周后，可以检测到针对传染性支气管炎病毒S1基因的ELISA抗体，重组病毒接种鸡的CD4^＋、CD8^＋和γδTCR阳性T淋巴细胞的百分比含量显著高于非免疫对照鸡（P％0．05）；免疫4周后用传染性支气管炎LX4强毒株攻击，rFPV—ChIFNγS1免疫组仅有1只出现轻微的呼吸道症状（1／16），而非免疫对照组则有16只发病（16／16），并有2只出现死亡（2／16），表明rFPV—ChIFNγS1对接种鸡可以产生良好的保护效果。  相似文献   

表达H3N2亚型猪流感病毒HA基因重组伪狂犬病病毒的构建   总被引：4，自引：1，他引：4  

周国辉  倪健强  田志军  仇华吉  李海燕  童光志 《畜牧兽医学报》2005,36(10):1043-1048

将SV40启动子控制下的LacZ基因表达盒和CMV启动子控制下的H3N2亚型猪流感病毒（SIV H3N2）的HA基因插入到伪狂犬病病毒（PRV）通用转移载体pBdTK-Uni中，获得转移载体pLTK-HA。将该载体与PRV Bartha-K61株基因组DNA通过脂质体法共转染Vero细胞，经过10代蓝斑筛选、纯化和PCR鉴定获得了一株插入SIV HA基因的重组伪狂犬病病毒，命名为rPRV-HA。Western blotting和间接免疫荧光试验证实HA基因在重组病毒感染的细胞中获得了表达。用不同的细胞（PK-15、IBRS-2、Vero和鸡胚成纤维细胞）对该重组病毒与亲本病毒的增殖滴度和致细胞病变进行比较，未见显著差异，对第30代重组病毒的HA基因进行序列分析，表明该重组病毒遗传性状稳定。  相似文献   

减氮施肥对菠菜生长发育及品质的影响   总被引：2，自引：0，他引：2  

佟容  王国臣  乔建磊  陈珊珊  吴春燕  于占东 《北方园艺》2021,(14):59-64

以菠菜为试材,采用随机区组方法,研究尿素不同施肥处理对菠菜生长发育的影响,以期达到菠菜生产合理施肥的目的 .结果 表明:适量的减氮施肥对菠菜植株生长发育及生理指标影响不显著,但过量的减施会显著影响菠菜的株高和产量.随着施氮量的减少,菠菜的维生素C和可溶性糖含量均显著增加.可溶性蛋白质含量有所下降,品种间菠菜硝酸盐含量下降及草酸含量下降,分别达到显著水平.综合比较,以"夏丽"品种在减氮20％处理下效果最佳.  相似文献   

西藏蔷薇科观赏植物资源及多样性研究     

汪海霞  吴彤  邢震  邓杰  禄树晖 《北方园艺》2015,(11):74-81

西藏拥有独特的高原自然环境条件和地理优势,为植物提供了较好的生长环境,孕育了许多具有特色的植物资源。采用野外实地踏查,采集、整理标本以及查阅大量文献等方法,对该区蔷薇科观赏植物资源及多样性进行了系统的研究。结果表明:该区共有蔷薇科观赏植物30属244种,是西藏种子植物区系中的第五大科,种数仅次于菊科、禾本科、豆科、杜鹃花科。其中,单种属有8属,占该区蔷薇科植物总属数26.67%;寡种属(含2~10种)有14属,占总属数46.67%;大型属(11种及以上)有8属,占总属数26.67%。水平分布上以藏东南高山深谷区的种数最多,有162种,占种数的66.94%;垂直分布上以海拔800~5 200m处为主。与四川、云南的相似性程度最高。该区以伯舒拉岭以北的三江河谷地带;尼洋河、帕龙藏布流域、易贡藏布中上游及雅鲁藏布江中游地区;喜马拉雅山脉东段南翼,伯舒拉岭以西的低山地带等暖湿带生境的丰富度最高。该区蔷薇科观赏植物地理分布类型共8种和4个分布区变形,温带成分优势突出,与北温带、旧世界温带、东亚都存在着联系;热带成分次之。该区蔷薇科观赏植物以观花观果类优势最为明显。研究认为,西藏蔷薇科观赏植物资源较丰富,对西藏地区寒冷、多岩石、土壤贫瘠等恶劣的生态环境来说,无疑是丰富局部区域生态群落结构、增加园林植物种类、提高绿化率优良植物。  相似文献   

芪合酶基因的研究进展与应用现状     

刘晶莹  佟少明  侯和胜 《天津农业科学》2015,(4):24-27

芪类化合物是一种重要的植物抗毒素,因其具有多种医疗保健作用,已引起多方关注。芪合酶是芪类化合物生物合成途径中的关键酶,关于它的研究已广泛开展。本文对芪类化合物的生物合成途径、药理作用,芪合酶的结构、功能,以及芪合酶基因的表达、芪合酶的基因工程研究等方面的进展进行综述,以期为进一步深入研究提供重要信息和科学参考。  相似文献   

松茸浓缩口服液制备过程中醇溶性物质的提取研究     

么宏伟  佟立君  付婷婷  谢晨阳  吴洪军  赵凤臣  冯磊  张学义 《中国食用菌》2015,(2):70-73

本实验是松茸口服液制备醇溶性物质提取部分。将超声波辅助水提取和弱碱盐溶液提取后的残渣用乙醇溶液提取,以多酚和三萜的总提取率为检测指标,对提取的条件进行了探讨。研究表明,提取的最优工艺为乙醇浓度80%,提取时间30 min,提取温度70℃,在此条件下多酚和三萜的得率分别为1.16 mg/100g和0.23 g/100g。  相似文献