Cultivation of corneal epithelial cell sheets on canine amniotic membrane     

Eunryel Nam  Ayaka Takahashi  Naoki Fujita  Keiko Tsuzuki  Ryohei Nishimura 《Veterinary ophthalmology》2013,16(4):263-268

Objective To develop and assess canine corneal epithelial cell sheets cultivated from limbal stem cells on amniotic membrane. Procedures Canine corneal limbal segments were obtained from six beagle dogs. Cryopreserved denuded amniotic membranes (obtained from Miniature Dachshund and Cavalier King Charles Spaniel breeds) from which the epithelial cells were removed were used as scaffolds. The limbal segments were cultured on these amniotic membranes with 3T3 feeder cells for 2 weeks. The harvested corneal epithelial cell sheets were stained with H&E for histologic analysis. The harvested sheets were analyzed immunohistochemically using a corneal epithelium‐specific marker keratin 3(K3) and putative stem cell markers ABCG2, p63, and vimentin. Results Cultivated cells from the corneal limbal tissues reached confluency in 7–8 days. The cultivated cells adhered to the denuded amniotic membrane and formed a sheet. The cultivated cell sheet was transparent and consisted of five to eight layers. K3 was observed in all layers and ABCG2, p63, and vimentin were notably present in the basal layer of the cultivated canine epithelium by immunofluorescence. Conclusions Canine corneal epithelial cells were successfully cultivated on the canine amniotic membrane. The cultivated epithelial sheets contained putative stem cells in the basal layer and had a stratified epithelium.  相似文献   

Feeding,somatic condition and survival of sand lance <Emphasis Type="Italic">Ammodytes</Emphasis> sp. larvae in Mutsu Bay,Japan     

Nobuaki Nanjo  Tetsuya Takatsu  Kazuo Imura  Kingo Itoh  Yuuhei Takeya  Toyomi Takahashi 《Fisheries Science》2017,83(2):199-214

To clarify the recruitment process of sand lance Ammodytes sp., we investigated larval condition factor, relative gut fullness (%GF), prey abundance and oceanographic structure in Mutsu Bay, Japan, during 1999–2001. Ammodytes sp. larvae, which were collected by horizontal hauls of Motoda nets and a ring net at depths of 1, 10, 20, 30 and 40 m, were mainly distributed at 10–30 m. Larvae at the first feeding time until 12 mm in body length (BL) fed predominantly on copepod nauplii, whereas large larvae with BL of 12.1–14.0 mm fed on a mixture of copepod nauplii, copepodites and appendicularians from late February to April. A path analysis showed that difference in water density between 35- and 5-m depths negatively affected naupliar abundance at 10–30-m depth (standardised path coefficient β = ?0.71, p = 0.005 for 3.3–8.0-mm BL larvae and β = ?0.78, p < 0.001 for 8.1–12.0-mm BL larvae). Naupliar abundance positively affected the %GF of Ammodytes sp. larvae (β = 0.75, p < 0.001 for 3.3–8.0-mm BL larvae and β = 0.66, p < 0.001 for 8.1–12.0-mm BL larvae), whereas it was negatively affected by water temperature (β = ?0.45, p = 0.008 for 3.3–8.0-mm BL larvae and β = ?0.56, p = 0.002 for 8.1–12.0-mm BL larvae), and the temperature effect was weak compared with that of naupliar abundance. In turn, %GF positively affected larval somatic weight (β = 0.91, p < 0.001 for 6.0-mm BL larvae and β = 0.70, p = 0.005 for 10.0-mm BL larvae). The recruitment failure in 1999 was likely caused by a reduced condition factor, which resulted from low naupliar abundance. In contrast, the abundance of nauplii and Oithona similis copepodites was high in 2000 and 2001. It is possible that the higher recruitment success in 2001 was because of the higher water temperatures in Mutsu Bay, sustaining faster growth of the larvae than in 2000 under the high-prey abundance conditions.  相似文献   

Development of Singlet Oxygen Absorption Capacity (SOAC) Assay Method. 3. Measurements of the SOAC Values for Phenolic Antioxidants     

K Mukai  A Ouchi  S Takahashi  K Aizawa  T Inakuma  J Terao  S Nagaoka 《Journal of agricultural and food chemistry》2012,60(32):7905-7916

Measurements of the singlet oxygen ((1)O(2)) quenching rates (k(Q) (S)) and the relative singlet oxygen absortpion capacity (SOAC) values were performed for 16 phenolic antioxidants (tocopherol derivatives, ubiquinol-10, caffeic acids, and catechins) and vitamin C in ethanol/chloroform/D(2)O (50:50:1, v/v/v) solution at 35 °C. It has been clarified that the SOAC method is useful to evaluate the (1)O(2)-quenching activity of lipophilic and hydrophilic antioxidants having 5 orders of magnitude different rate constants from 1.38 × 10(10) M(-1) s(-1) for lycopene to 2.71 × 10(5) for ferulic acid. The logarithms of the k(Q) (S) and the SOAC values for phenolic antioxidants were found to correlate well with their peak oxidation potentials (E(p)); the antioxidants that have smaller E(p) values show higher reactivities. In previous works, measurements of the k(Q) (S) values for many phenolic antioxidants were performed in ethanol. Consequently, measurements of the k(Q) (S) and relative SOAC values were performed for eight carotenoids in ethanol to investigate the effect of solvent on the (1)O(2)-quenching rate. The k(Q) (S) values for phenolic antioxidants and carotenoids in ethanol were found to correlate linearly with the k(Q) (S) values in ethanol/chloroform/D(2)O solution with a gradient of 1.79, except for two catechins. As the relative rate constants (k(Q)(AO) (S)/k(Q)(α-Toc) (S)) of antioxidants (AO) are equal to the relative SOAC values, the SOAC values do not depend on the kinds of solvent used, if α-tocopherol is used as a standard compound. In fact, the SOAC values obtained for carotenoids in mixed solvent agreed well with the corresponding ones in ethanol.  相似文献   

Effects of warm-up intensity on oxygen transport during supramaximal exercise in horses     

Mukai K  Hiraga A  Eto D  Takahashi T  Hada T  Tsubone H  Jones JH 《American journal of veterinary research》2008,69(5):690-696

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Exogenous application of l-histidine suppresses bacterial diseases and enhances ethylene production in rice seedlings     

S. Yariyama  S. Ando  S. Seo  K. Nakaho  S. Miyashita  Y. Kanayama  H. Takahashi 《Plant pathology》2019,68(6):1072-1078

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Possible involvement of cyclic AMP as an inhibitory regulator in thrombocyte aggregation induced by collagen in carp <Emphasis Type="Italic">Cyprinus carpio</Emphasis>     

Teruo Matsushita  Shin -Ichi Inoue  Ryusuke Tanaka  Masakazu Kondo  Yukinori Takahashi  Mitsuko Miyamoto  Hajimu Kurumaiani  Toshio Nakaki 《Fisheries Science》2008,74(1):214-216

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Evaluation from otolith Sr stable isotope ratios of possible juvenile growth areas of Japanese eels collected from the West Mariana Ridge spawning area     

Otake  Tsuguo  Amano  Yosuke  Shirai  Kotaro  Mochioka  Noritaka  Takahashi  Toshiro  Chow  Seinen  Kurogi  Hiroaki  Dou  Shouzeng  Yamaguchi  Asuka  Tsukamoto  Katsumi 《Fisheries Science》2019,85(3):483-493

Fisheries Science - The widely distributed East Asian Japanese eel Anguilla japonica constitutes a single genetically homogeneous population with a single spawning area near the West Mariana Ridge....  相似文献   

Environmental characteristics of spawning and nursery grounds of Japanese sardine and mackerels in the Kuroshio and Kuroshio Extension area     

Sayaka Sogawa  Kiyotaka Hidaka  Yasuhiro Kamimura  Masanori Takahashi  Hiroaki Saito  Yuji Okazaki  Yugo Shimizu  Takashi Setou 《Fisheries Oceanography》2019,28(4):454-467

The sustainable use of marine resources requires understanding the surrounding ecosystem and elucidating mechanisms of variation. However, we still lack a comprehensive understanding of environmental variation in the spawning and nursery grounds of important fisheries species Japanese sardine (Sardinops melanostictus) and mackerels (Scomber japonicus and Scomber australasicus) in the northwest Pacific. Here, we investigate detailed physical, chemical, and biological environment variations in the spawning and nursery grounds along the Kuroshio and Kuroshio Extension area from intensive investigation in spawning season (April) of 2013. We found similar water mass property and copepod community in the egg‐rich Kuroshio area and the larvae‐rich downstream Kuroshio Extension area, indicating environmental variability is small during transportation and development processes. The egg‐rich northern Izu Islands region showed high copepod abundance, although low nutrient and chlorophyll concentrations were observed. Eggs were scarce or absent in the second survey 10 days after abundant eggs were observed in the region, along with differences in water property and copepod community. This indicates that not only the location but also the specific water characteristic and copepod community are a determining factor for spawning. Indicator communities of copepod found in our study (indicator community of transportation process from spawning ground, of non‐spawning ground, and of reproductive area in the Kuroshio Extension area) would be a key factor for recruitment prediction.  相似文献   

The use of machine learning to predict acute hepatopancreatic necrosis disease (AHPND) in shrimp farmed on the east coast of the Mekong Delta of Vietnam     

Khiem  Nguyen Minh  Takahashi  Yuki  Oanh  Dang Thi Hoang  Hai  Tran Ngoc  Yasuma  Hiroki  Kimura  Nobuo 《Fisheries Science》2020,86(4):673-683

Fisheries Science - Predicting the outbreak of disease is essential when managing shrimp farms. Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus is a serious...  相似文献   

Chemical characteristics of surfaces of hardwood and softwood deteriorated by weathering   总被引：3，自引：1，他引：2  

Yanni Sudiyani  Sho-ichi Tsujiyama  Yuji Imamura  Munezoh Takahashi  Kazuya Minato  Hiromu Kajita 《Journal of Wood Science》1999,45(4):348-353

The factors that cause weather-induced deterioration of wood surfaces were determined by chemical and spectroscopic analyses. Albizzia (Paraserianthes falcata Becker.) and sugi (Cryptomeria japonica D. Don) were exposed to two temperate conditions of natural weathering with and without rainfall and to accelerated conditions of artificial weathering coupled with ultraviolet (UV) light irradiation and water flashing. Infrared spectroscopic analysis showed that the oxidative reaction of lignin was observed under all conditions of weathering for both wood species. However, a marked decrease in lignin and hemicellulose content were recognized when albizzia woods were exposed to weathering with water. Lignin content in the softwood sugi did not decrease as much as in albizzia even in the presence of water, but the modification of lignin macromolecules was assumed to be accelerated by water, as seen by electron spin resonance spectroscopy. These results showed that the presence of water promotes the weathering deterioration of wood under UV irradiation.  相似文献