Development of reverse transcriptase PCR and nested PCR to detect porcine hemagglutinating encephalomyelitis virus   总被引：4，自引：0，他引：4  

Sekiguchi Y  Shirai J  Taniguchi T  Honda E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):367-372

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Mass and local topography measurements of Itokawa by Hayabusa     

Abe S  Mukai T  Hirata N  Barnouin-Jha OS  Cheng AF  Demura H  Gaskell RW  Hashimoto T  Hiraoka K  Honda T  Kubota T  Matsuoka M  Mizuno T  Nakamura R  Scheeres DJ  Yoshikawa M 《Science (New York, N.Y.)》2006,312(5778):1344-1347

The ranging instrument aboard the Hayabusa spacecraft measured the surface topography of asteroid 25143 Itokawa and its mass. A typical rough area is similar in roughness to debris located on the interior wall of a large crater on asteroid 433 Eros, which suggests a surface structure on Itokawa similar to crater ejecta on Eros. The mass of Itokawa was estimated as (3.58 +/- 0.18) x 10(10) kilograms, implying a bulk density of (1.95 +/- 0.14) grams per cubic centimeter for a volume of (1.84 +/- 0.09) x 10(7) cubic meters and a bulk porosity of approximately 40%, which is similar to that of angular sands, when assuming an LL (low iron chondritic) meteorite composition. Combined with surface observations, these data indicate that Itokawa is the first subkilometer-sized small asteroid showing a rubble-pile body rather than a solid monolithic asteroid.  相似文献   

Identification of bovine dendritic cell phenotype from bovine peripheral blood     

Miyazawa K  Aso H  Honda M  Kido T  Minashima T  Kanaya T  Watanabe K  Ohwada S  Rose MT  Yamaguchi T 《Research in veterinary science》2006,81(1):40-45

Dendritic cells (DCs) are professional antigen presenting cells, which initiate primary immune responses and also play an important role in the generation of peripheral tolerance. There is no reliable method established for the isolation of bovine peripheral blood DCs, and furthermore, the phenotypes and the functions of bovine DCs are still not fully clear. In the present study, we have attempted to identify bovine peripheral blood DCs by negative-selection. In bovine peripheral blood mononuclear cells (PBMC), we have newly characterized the phenotype of DCs, which is CD11c+/CD172a+. These cells display features of myeloid type DCs. In the thymic medulla, CD11c+/CD172a+ cells were also present and CD1+/CD172a+ cells were additionally detected as a population of DCs. The data suggest that one of the bovine DCs phenotypes from PBMC is derived from myeloid lineages lacking a CD1 molecule, which then drift to several tissues, and that they then may express a CD1 molecule upon their functional differentiation.  相似文献   

The W‐ and Z‐linked EE0.6 sequences used for molecular sexing of captive Japanese crested ibis on Sado Island     

Kaori KASUGA  Maho HIGASHI  Takahisa YAMADA  Toshie SUGIYAMA  Yukio TANIGUCHI  Hiroaki IWAISAKI  Kosuke HOMMA  Yuuichi WAJIKI  Yoshinori KANEKO  Satoshi YAMAGISHI 《Animal Science Journal》2012,83(1):83-87

The Japanese crested ibis Nipponia nippon is a critically threatened bird. Accurate sexing is necessary to perform effective management of captive breeding toward a national project for a tentative release of the Japanese crested ibis on Sado Island. A PCR‐based sexing method targeting a 0.6 kb EcoRI fragment (EE0.6) sequence on W chromosome with AWS03 and USP3 primers has been developed for the Japanese crested ibis. However, the primers were selected from the EE0.6 sequences from bird species other than the Japanese crested ibis. In this study, we determined the W‐ and Z‐linked EE0.6 sequences in the Japanese crested ibis, and clarified Japanese crested ibis sequence mismatch in the binding sites of the primers. Further, we found no polymorphism in the primer binding sites among five founder birds for the Sado captive Japanese crested ibis population. These findings validated the PCR‐based sexing method with the AWS03 and USP3 as accurate molecular sexing methods of captive Japanese crested ibis on the Sado Island. Additionally, we designed a primer set for a novel PCR‐based sexing, based on the EE0.6 sequences obtained in this study. This novel sexing method may be useful for future ecological research following the release of Japanese crested ibis on Sado Island. This is the first report to show the EE0.6 sequences in Japanese crested ibis.  相似文献   

Importance of shingle beaches as habitat for Manila clam Ruditapes philippinarum     

Ichimi  Kazuhiko  Honda  Miyuki  Okada  Yuka  Tsuzuki  Kana  Yamaguchi  Hitomi 《Fisheries Science》2019,85(3):417-427

Fisheries Science - We carried out surveys to clarify how the density (no. of individuals/m2) and condition factor (CF) of Manila clam Ruditapes philippinarum differ between estuarine tidal flats...  相似文献   

Etiologic study of upper respiratory infections of household dogs     

Mochizuki M  Yachi A  Ohshima T  Ohuchi A  Ishida T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):563-569

Infectious tracheobronchitis (ITB), also known as the kennel cough, is a respiratory syndrome of dogs and usually appears to be contagious among dogs housed in groups. Etiologic agent of ITB is multiple and sometimes complex. In the present study, 68 household dogs showing clinical signs of respiratory infection were examined, and 20 dogs (29.4%) were found to be positive for either of following agents. Bordetella bronchiseptica (B.b.) was most frequently detected from nasal and oropharynx sites of 7 dogs (10.3%). Among the viruses examined, canine parainfluenza virus (CPIV) was detected with the highest frequency (7.4%). Other pathogens included in the order of frequency group 1 canine coronavirus (4.4%), canine adenovirus type 2 (2.9%), group 2 canine respiratory coronavirus (1.5%), and canine distemper virus (1.5%). Only 2 cases showed mixed infections. Neither influenza A virus nor canine bocavirus (minute virus of canines) was found in any dogs examined. These results indicate that both B.b. and CPIV are likely to be the principal etiologic agents of canine ITB in Japan, and they may be considered as the target for prophylaxis by vaccination.  相似文献   

Pathological characteristics of thyroid glands from Japanese Black Cattle living in the restricted area of the Fukushima Daiichi Nuclear Power Plant accident     

Jun Sasaki  Megumi Uehara  Itaru Sato  Hiroshi Satoh  Yoshitaka Deguchi  Hiroyuki Chida  Masahiro Natsuhori  Takahisa Murata  Kenji Ochiai  Kumiko Otani  Keiji Okada  Nobuhiko Ito 《Animal Science Journal》2019,90(9):1333-1339

To study the effect of ionizing radiation on thyroid glands, 66 Japanese Black cattle residing in the restricted area of the Fukushima Daiichi Nuclear Power Plant in 2013–2017 were pathologically examined. There were no clinical symptoms of thyroid disease in these cattle. Three cases of goiter and seven of atrophy were found in two among the four farms examined. Cases of goiter exhibited normal morphological structure without mass or nodule formation in thyroid glands. Cellular atypia or capsular invasion of the follicular epithelium was absent. The estimated integrated dose of external radiation in goiter cases ranged from maximum 797 mSv to minimum 24 mSv. All lobules in the seven atrophic thyroid glands were affected, but pathological findings, such as inflammatory cell infiltration or stromal fibrosis, were not observed. The estimated integrated dose of external radiation in atrophic thyroids ranged from maximum 589 mSv to minimum 8 mSv. Immunohistochemical analysis of anti‐nitroguanosine and the TUNEL method in goiter and atrophic thyroid glands did not reveal any positive findings. The present study indicates that there was no significant relationship between a radiation effect and pathological findings in any thyroid glands.  相似文献   

Comparison of urine and blood as a convenient and practical sample for estimating the contamination level of live cattle with radioactive cesium     

Itaru Sato  Jun Sasaki  Hiroshi Satoh  Masahiro Natsuhori  Takahisa Murata  Keiji Okada 《Animal Science Journal》2019,90(8):1090-1095

Blood and urine were compared to clarify which is a better sample for estimating the concentration of radioactive cesium in muscles of live cattle. The most probable concentration of ¹³⁷Cs in muscles was 21.0 times that in blood, and the error of this estimation was 28%. The concentration of ¹³⁷Cs in blood was estimated accurately using urine samples. The estimation error did not increase so much (33%), even when the concentration of ¹³⁷Cs in muscles was estimated using urine samples. On the other hand, the maximum volume of blood that can be collected with one syringe is 50 ml, whereas it is easy to collect 1,000 ml of urine. It took at least 360 min to confirm that a cow meets the legal standard by inspecting 50 ml of blood. However, with 1,000 ml of urine, a 20 min measurement time was sufficient for this purpose. This difference in the required measurement time is critically important for practical use. In addition, urine can be collected by farmers themselves, whereas the blood collection requires a veterinarian. Therefore, urine is a more convenient and practical sample for estimating the contamination level of live cattle with radioactive cesium.  相似文献   

Management and yield prediction of Kunugi (Quercus acutissima) grazing forests     

Mitsuo Matsumoto  Kenjiro Honda  Juuro Kurogi 《Journal of Forest Research》1999,4(2):61-66

This study deals with grazing in kunugi (Quercus acutissima) forests in the Aso district of Kyushu Island in southwest Japan. These forests are managed for production of bed-logs for shiitake mushrooms and cow-calf farming. One of their characteristics is short-term rotation such as 10–15 years for bed-logs and a year for calf production. A forest grazing experiment was begun in Minamioguni to look at forest growth, vegetation change and grazing intensity. Stem densities dropped in a few years. After sprout cutting, they also dropped gradually, then stabilized. Although grazing caused tree damage and suppressed tree growth, grazing intensity of up to 150 cow-days/ha·year did not harm forest regeneration. Herbage volume decreased as grazing was repeated and trees grew. Another investigation of kunugi grazing forests in Minamioguni and Asaji showed the correlation betweenRy (yield index in Stand density diagram) and grazing capacity could be expressed with a regression equation. The results were also used to design a yield table for kunugi grazing forests. The yield table has items ofRy and grazing capacity in addition to usual yield table items, and can indicate timber yield and grazing capacity at the same time. The table estimates that proper grazing capacity is 60–80 cow-days/ha·year in wild grass sites. In the light of these results, an optimal management plan was proposed as a diagram integrating stem density, forest yield, and forest management.  相似文献   

Growth,nutrient utilization,oxidative condition,and element composition of juvenile red sea bream <Emphasis Type="Italic">Pagrus major</Emphasis> fed with fermented soybean meal and scallop by-product blend as fishmeal replacement     

Md. Abdul Kader  Shunsuke Koshio  Manabu Ishikawa  Saichiro Yokoyama  Mahbuba Bulbul  Yuki Honda  Roger Edward Mamauag  Asda Laining 《Fisheries Science》2011,77(1):119-128

A feeding trial was conducted to evaluate the effect of fermented soybean meal and scallop by-product blend (3:2) (FP) on the performance of juvenile red sea bream. Five isocaloric diets were prepared by replacing 0% (FP0), 15% (FP15), 30% (FP30), 45% (FP45), and 60% (FP60) fishmeal (FM) protein with FP, respectively. Triplicate groups of fish (initial mean weight 2.83 g) were fed the test diets for 45 days in a flowthrough seawater system. The results demonstrated that growth rates of fish fed FP0, FP15, and FP30 were similar, and significantly higher (P < 0.05) than those of FP45 and FP60. Nutrient utilization was significantly lower in FP60, with no differences found among the other groups. Dietary heavy-metal contents were affected by inclusion of FP, impacting on whole-body heavy-metal contents. In terms of oxidative stress, fish fed the FP30 diet were in the best condition, since this fish group showed the least oxidative-stressed condition as well as the highest tolerance against oxidation. In conclusion, the approach of utilizing this fermented mixture is promising and it could replace at least 30% FM protein in red sea bream diet without negative effects on performance, body composition or health of fish.  相似文献