B‐cell lymphoma with Mott cell differentiation in a cat          下载免费PDF全文

Tomomi Kanehara  Naoko Matsui  Mami Murakami  Kohji Maruo  Takashi Mori  Akihiro Hirata  Tokuma Yanai  Hiroki Sakai 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(2):356-360

A 12‐year‐old, male castrated Domestic Shorthair cat was presented to Animal Medical Center of Gifu Univeristy with anorexia and vomiting. Physical examination revealed an enlarged left tonsil and right mandibular lymph node (approximately 2–3× the normal size), and a submucosal mass on the right side of the epiglottis (1.5 × 2.0 cm). On computed tomography images, an enlarged left tonsil, and enlarged right mandibular, right pharyngeal, and left and right cervical lymph nodes were observed. Cytologic examination of smears of tonsil and lymph nodes revealed numerous medium‐ to large‐sized neoplastic lymphoid cells, approximately half of which contained one or several light‐blue homogenous globoid cytoplasmic inclusions (5–10 μm), which stained magenta with periodic acid–Schiff (PAS) stain. Histopathologic examination of the left tonsil revealed diffuse proliferation of medium‐ to large‐sized neoplastic lymphoid cells effacing the original lymphoid architecture. Half of the cells contained one or several eosinophilic globoid cytoplasmic inclusions, which stained magenta with PAS and showed positive immunohistochemical reactions for immunoglobulin M (IgM) and λ light chain. Neoplastic lymphoid cells were also CD20⁺, Pax5⁺, and MUM1⁺, and CD3^?. Thus, the neoplastic lymphoid cells expressed a B‐cell immunophenotype, and the globoid cytoplasmic inclusions represented an aberrant IgM λ light chain accumulation, similar to Russell bodies. B‐cell lymphoma with Mott cell differentiation was diagnosed based on cytologic, histopathologic, and immunohistochemical features. This is the first report of B‐cell lymphoma with Mott cell differentiation in a cat.  相似文献   

Identification of QTL controlling post-flowering period in soybean     

Kunihiko Komatsu  Tae-Young Hwang  Masakazu Takahashi  Takashi Sayama  Hideyuki Funatsuki  Nobuhiko Oki  Masao Ishimoto 《Breeding Science》2012,61(5):646-652

The length of the reproductive period affects the grain yield of soybean (Glycine max [L.] Merr), and genetic control of the period might contribute to yield improvement. To detect genetic factor(s) controlling the reproductive period, a population of recombinant inbred lines (RILs) was developed from a cross between Japanese landrace ‘Ippon-Sangoh’ and, Japanese cultivar ‘Fukuyutaka’ which differ in their duration from flowering to maturation (DFM) relative to the difference in the duration from sowing to flowering (DSF). In the RIL population, the DFM correlated poorly (r = −0.16 to 0.34) with the DSF in all field trials over 3 years. Two stable QTLs for the DFM on chromosomes (Chr-) 10 and 11 as well as two stable QTLs for the DSF on Chr-10 and -16 were identified. The QTL on Chr-11 for the reproductive period (designated as qDfm1; quantitative trait locus for duration from flowering to maturation 1) affected all three trials, and the difference in the DFM between the Fukuyutaka and Ippon-Sangoh was mainly accounted for qDfm1, in which the Fukuyutaka allele promoted a longer period. qDfm1 affected predominantly the reproductive period, and thus it might be possible to alter the period with little influence on the vegetative period.  相似文献   

DaizuBase,an integrated soybean genome database including BAC-based physical maps     

Yuichi Katayose  Hiroyuki Kanamori  Michihiko Shimomura  Hajime Ohyanagi  Hiroshi Ikawa  Hiroshi Minami  Michie Shibata  Tomoko Ito  Kanako Kurita  Kazue Ito  Yasutaka Tsubokura  Akito Kaga  Jianzhong Wu  Takashi Matsumoto  Kyuya Harada  Takuji Sasaki 《Breeding Science》2012,61(5):661-664

Soybean [Glycine max (L) Merrill] is one of the most important leguminous crops and ranks fourth after to rice, wheat and maize in terms of world crop production. Soybean contains abundant protein and oil, which makes it a major source of nutritious food, livestock feed and industrial products. In Japan, soybean is also an important source of traditional staples such as tofu, natto, miso and soy sauce. The soybean genome was determined in 2010. With its enormous size, physical mapping and genome sequencing are the most effective approaches towards understanding the structure and function of the soybean genome. We constructed bacterial artificial chromosome (BAC) libraries from the Japanese soybean cultivar, Enrei. The end-sequences of approximately 100,000 BAC clones were analyzed and used for construction of a BAC-based physical map of the genome. BLAST analysis between Enrei BAC-end sequences and the Williams82 genome was carried out to increase the saturation of the map. This physical map will be used to characterize the genome structure of Japanese soybean cultivars, to develop methods for the isolation of agronomically important genes and to facilitate comparative soybean genome research. The current status of physical mapping of the soybean genome and construction of database are presented.  相似文献   

Genetic analysis of antixenosis resistance to the common cutworm (Spodoptera litura Fabricius) and its relationship with pubescence characteristics in soybean (Glycine max (L.) Merr.)     

Nobuhiko Oki  Kunihiko Komatsu  Takashi Sayama  Masao Ishimoto  Masakazu Takahashi  Motoki Takahashi 《Breeding Science》2012,61(5):608-617

The common cutworm (CCW, Spodoptera litura Fabricius) is one of the most serious pests of soybean (Glycine max (L.) Merr.). Previously, two quantitative trait loci (QTLs) for antibiosis resistance to CCW, CCW-1 and CCW-2, were detected in the resistant cultivar Himeshirazu. In this study, we conducted an anti-xenosis bioassay using a recombinant inbred population derived from a cross between a susceptible cultivar Fukuyutaka and Himeshirazu to perform QTL analysis. Two QTLs for antixenosis resistance, qRslx1 and qRslx2, were identified on Chrs 7 and 12, and the resistant alleles of qRslx1 and qRslx2 were derived from Himeshirazu and Fukuyutaka, respectively. The position of qRslx1 is similar to that of CCW-1. We also analyzed pubescence characteristics because they have been reported to be associated with soybean insect resistance. Two QTLs for pubescence length (on Chrs 7 and 12) and two QTLs for pubescence density (on Chrs 1 and 12) were identified. The pubescence QTLs on Chrs 7 and 12 were located near qRslx1 and qRslx2, respectively. These results suggest that the antixenosis resistance could be controlled genetically by the identified QTLs and that the pubescence characteristics might contribute to the soybean antixenosis resistance to CCW.  相似文献   

Iconic CO2 time series at risk     

S Houweling  B Badawy  DF Baker  S Basu  D Belikov  P Bergamaschi  P Bousquet  G Broquet  T Butler  JG Canadell  J Chen  F Chevallier  P Ciais  GJ Collatz  S Denning  R Engelen  IG Enting  ML Fischer  A Fraser  C Gerbig  M Gloor  AR Jacobson  DB Jones  M Heimann  A Khalil  T Kaminski  PS Kasibhatla  NY Krakauer  M Krol  T Maki  S Maksyutov  A Manning  A Meesters  JB Miller  PI Palmer  P Patra  W Peters  P Peylin  Z Poussi  MJ Prather  JT Randerson  T Röckmann  C Rödenbeck  JL Sarmiento  DS Schimel  M Scholze  A Schuh  P Suntharalingam 《Science (New York, N.Y.)》2012,337(6098):1038-1040

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Surface plasmon resonance analysis on interactions of food components with a taste epithelial cell model     

Miyano M  Yamashita H  Sakurai T  Nakajima K  Ito K  Misaka T  Ishimaru Y  Abe K  Asakura T 《Journal of agricultural and food chemistry》2010,58(22):11870-11875

A new device for evaluating the continuity of taste was developed with the use of surface plasmon resonance (SPR). The model of lingual cells was constructed with liposomes immobilized onto an L1 sensor chip for SPR. Using this device, we classified food components into three categories according to the sensorgram pattern and residual ratio on lipid bilayer. Samples in group A strongly interacted with lipid bilayer, those in group B poorly interacted, and those in group C belong to neither group A nor group B. Sweet proteins and gymnemic acids that prolonged sweet perception were categorized in group A. Almost all the carbohydrates investigated and aspartame, of which the taste perception does not continue, belonged to group B. This device made it possible to detect the interaction with lipid bilayer and dissected the mechanism of taste continuity.  相似文献   

Tocopherol affinity for serum lipoproteins of Japanese flounder Paralichthys olivaceus during the reproduction period     

Masaharu Tokuda  Toshiyasu Yamaguchi  Kunihiro Wakui  Toshihiko Sato  Masao Ito and  Masaaki Takeuchi 《Fisheries Science》2000,66(4):619-624

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Detection of rabies virus RNA isolated from several species of animals in Brazil by RT-PCR.   总被引：9，自引：0，他引：9  

M Ito  T Itou  T Sakai  M F Santos  Y T Arai  T Takasaki  I Kurane  F H Ito 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(12):1309-1313

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Effect of an automatic milking system on milking traits     

Takashi HAYASHI  Teruo KAWAMURA 《Animal Science Journal》2002,73(5):403-408

Recent studies in Japan of the effect of automatic milking systems (AMS) on the behavior of dairy cows have confirmed the labor-saving aspect of AMS, but have not solved some of the problems affecting milking traits. The influence of AMS on milking was examined in 39 Holstein cows at Iwate Agricultural Research Center from June 1998 to December 1999, by measuring and recording the yield per milking, milking finish time, milking frequency, and daily yield of milking. Four milking traits were analyzed separately as the objective variable in a linear model, with parity, days in milking, month after installation of the AMS as independent variables. Primiparous cows milked more frequently in the middle and late stage of lactation, yielded less per milking and less per day in the early stage of lactation than multiparous cows with the AMS. Lower frequencies were observed at noon and midnight for cleaning of the bulk tank. By contrast, higher milking frequency in the forenoon was shown. The yield per milking rose and declined, following a normal lactation curve. However, milking frequency rose slowly, and declined gradually in the late stage of lactation. After installing a one-way gate, the cows milked more frequently, with a lower yield per milking and a greater daily milk yield, than before installation.  相似文献   

Quantitative trait loci for whiteness of cooked rice detected in improved rice cultivars in Hokkaido     

Hiroshi Shinada  Toshio Yamamoto  Eiji Yamamoto  Kiyosumi Hori  Yuji Hirayama  Toshihiko Maekawa  Hitoshi Kiuchi  Hirokazu Sato  Takashi Sato 《Breeding Science》2015,65(3):201-207

Improving the eating quality of cooked rice has been one of the most important objectives in rice breeding programs. Eating quality of cooked rice is a complex trait including several components, such as external appearance, taste, aroma, and texture. Therefore, dissection of these components followed by marker-assisted selection of detected QTL(s) may be a useful approach for achieving desirable eating quality in rice breeding. Whiteness of cooked rice (WCR) is an important factor related to the external appearance of cooked rice. WCR is known to be associated with the amylose and protein contents of the endosperm. However, the genetic basis of WCR remains unclear. In this study, we evaluated phenotypic variation in WCR among recently developed rice cultivars from Hokkaido, Japan. Then, we developed doubled haploid lines (DHLs) derived from a cross between two cultivars from Hokkaido, Joiku No. 462 (high WCR) and Jokei06214 (low WCR). Using the DHLs, we detected two QTLs for WCR, qWCR3 and qWCR11, on chromosomes 3 and 11, respectively. We also examined the dosage effect of the two QTLs based on both the categorized segregants in the DHLs and the relationship between the WCR phenotype and inheritance around the QTL regions in cultivars from Hokkaido.  相似文献