Comparison of the RNA polymerase I-, II- and III-dependent transcript levels between nuclear transfer and in vitro fertilized embryos at the blastocyst stage     

Suzuki T  Minami N  Kono T  Imai H 《The Journal of reproduction and development》2007,53(3):663-671

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A novel culture method of canine peripheral blood lymphocytes with concanavalin a and recombinant human interleukin-2 for adoptive immunotherapy   总被引：1，自引：0，他引：1  

Kato M  Watarai S  Nishikawa S  Iwasaki T  Kodama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):481-486

This study was designed to develop a novel culture method for the efficient proliferation of canine peripheral blood lymphocytes (cPBL) for adoptive immunotherapy. When cPBL were cultured in the presence of concanavalin A (Con A), proliferation of cPBL was induced and expression of interleukin-2 receptor (IL-2R) which enables to respond to exogenously added IL-2 was upregulated. And then, when cPBL were cultured with recombinant human interleukin-2 (rhIL-2) in addition to Con A, proliferation was accelerated and increased to about 10-fold after 1 week. The phenotypic analysis showed that the main population of the cultured cPBL was consisted of CD8+ positive lymphocytes. Among them, CD4+CD8+ double positive (DP) lymphocytes had significantly increased, and the ratio of CD4+ single positive (SP) lymphocytes to CD8+ SP lymphocytes (CD4+SP/CD8+SP) was decreased as compared to before culturing. To evaluate the cytotoxic activity of cPBL cultured with Con A and rhIL-2, furthermore, cytotoxic assay was carried out against xenogeneic melanoma cell line (MeWo), which resulted in MHC-unrestricted cytokilling. These results suggest that the culture method of cPBL by the use of Con A and rhIL-2 may be useful for generating lymphokine activated killer cells, and also this may be beneficial for adoptive immunotherapy of tumor-bearing dogs.  相似文献   

Prior infection with antigenically heterologous low pathogenic avian influenza viruses interferes with the lethality of the H5 highly pathogenic strain in domestic ducks     

Kosuke SODA  Maya YAMANE  Chiharu HIDAKA  Kozue MIURA  Trang T. H. UNG  Hang L. K. NGUYEN  Hiroshi ITO  Mai Q. LE  Toshihiro ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1899

Low and highly pathogenic avian influenza viruses (LPAIVs and HPAIVs, respectively) have been co-circulating in poultry populations in Asian, Middle Eastern, and African countries. In our avian-flu surveillance in Vietnamese domestic ducks, viral genes of LPAIV and HPAIV have been frequently detected in the same individual. To assess the influence of LPAIV on the pathogenicity of H5 HPAIV in domestic ducks, an experimental co-infection study was performed. One-week-old domestic ducks were inoculated intranasally and orally with phosphate-buffered saline (PBS) (control) or 10⁶ EID₅₀ of LPAIVs (A/duck/Vietnam/LBM678/2014 (H6N6) or A/Muscovy duck/Vietnam/LBM694/2014 (H9N2)). Seven days later, these ducks were inoculated with HPAIV (A/Muscovy duck/Vietnam/LBM808/2015 (H5N6)) in the same manner. The respective survival rates were 100% and 50% in ducks pre-infected with LBM694 or LBM678 strains and both higher than the survival of the control group (25%). The virus titers in oral/cloacal swabs of each LPAIV pre-inoculation group were significantly lower at 3–5 days post-HPAIV inoculation. Notably, almost no virus was detected in swabs from surviving individuals of the LBM678 pre-inoculation group. Antigenic cross-reactivity among the viruses was not observed in the neutralization test. These results suggest that pre-infection with LPAIV attenuates the pathogenicity of HPAIV in domestic ducks, which might be explained by innate and/or cell-mediated immunity induced by the initial infection with LPAIV.  相似文献   

Contribution of mutation I142M in fusion protein and Q44R in matrix protein of Newcastle disease virus to virulence in ducks     

Chiharu HIDAKA  Kosuke SODA  Toshihiro ITO  Hiroshi ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):121

Although verogenic Newcastle disease viruses (NDVs) generally cause subclinical infection in waterfowls such as ducks, NDVs with high virulence in waterfowl have been sporadically reported. We previously reported that the NDV d5a20b strain, which is obtained by serial passaging of the velogenic 9a5b strain in domestic ducks, showed increased virulence in ducks (Hidaka et al., 2021). The d5a20b strain had 11 amino acid substitutions in its P/V, M, F, HN, and L proteins as compared to 9a5b. In the present study, we generated a series of recombinant (r) NDVs with these amino acid substitutions to identify the molecular basis of virulence of NDV in ducks, and evaluated their influences on virulence and in vitro viral properties. Each of the single amino acid substitutions in either the F protein I142M or the M protein Q44R contributed to the enhancement of intracerebral and intranasal pathogenicity in domestic ducks. The cell-cell fusion activity of the virus with F I142M was five times higher than that of the parental r9a5b. The virus with M Q44R rapidly replicated in duck embryo fibroblasts. Additionally, the rM+F+HN strain, which has the same amino acid sequences as d5a20b in M, F, and HN proteins, showed the highest level of virulence and replication efficiency among the generated recombinant viruses, nearly comparable to rd5a20b. These results suggest that multiple factors are involved in the high growth ability of NDV in duck cells, leading to increased virulence in vivo.  相似文献   

GM1 gangliosidosis in a Japanese domestic cat: a new variant identified in Hokkaido,Japan     

Hiroshi UENO  Osamu YAMATO  Takeshi SUGIURA  Moeko KOHYAMA  Akira YABUKI  Kenjiro MIYOSHI  Kazuya MATSUDA  Tsuyoshi UCHIDE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):91-95

A male Japanese domestic cat with retarded growth in Hokkaido, Japan, showed progressive motor dysfunction, such as ataxia starting at 3 months of age and tremors, visual disorder and seizure after 4 months of age. Finally, the cat died of neurological deterioration at 9 months of age. Approximately half of the peripheral blood lymphocytes had multiple abnormal vacuoles. Magnetic resonance imaging showed bisymmetrical hyperintensity in the white matter of the parietal and occipital lobes in the forebrain on T2-weighted and fluid-attenuated inversion recovery images, and mild encephalatrophy of the olfactory bulbs and temporal lobes. The activity of lysosomal acid β-galactosidase in leukocytes was negligible, resulting in the biochemical diagnosis of GM1 gangliosidosis. Histologically, swollen neurons characterized by accumulation of pale, slightly granular cytoplasmic materials were observed throughout the central nervous system. Dysmyelination or demyelination and gemistocytic astrocytosis were observed in the white matter. Ultrastructually, membranous cytoplasmic bodies were detected in the lysosomes of neurons. However, genetic analysis did not identify the c.1448G>C mutation, which is the single known mutation of feline GM1 gangliosidosis, suggesting that the cat was affected with a new variant of the feline disease.  相似文献   

Screening for Small Molecule Inhibitors of BMP-Induced Osteoblastic Differentiation from Indonesian Marine Invertebrates     

Hiroyuki Yamazaki  Satoshi Ohte  Henki Rotinsulu  Defny S. Wewengkang  Deiske A. Sumilat  Delfly B. Abdjul  Wilmar Maarisit  Magie M. Kapojos  Michio Namikoshi  Takenobu Katagiri  Hiroshi Tomoda  Ryuji Uchida 《Marine drugs》2020,18(12)

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Evaluation of stock management procedures for walleye pollock in northern waters of the Sea of Japan using a simulation     

Hiroshi Yamaguchi  Takashi Matsuishi 《Fisheries Science》2008,74(5):955-969

To evaluate walleye pollock stock management procedures in the northern waters of the Sea of Japan, 30-year population dynamics, including uncertainties, were forecast. Errors in current stock size estimation, variability in future recruitment and changes in future fishing mortalities were incorporated. Results of virtual population analysis (VPA) from resampled catch-at-age data with bootstrap methods was used as the current stock size estimation with uncertainty. Performances of each scenario were evaluated using conservation, utilization, stability and reliability factors. Twenty-two management scenarios and continuing the current fishing mortality were evaluated. Scenarios with minor regulation changes and continuing the current fishing mortality showed poor stock conservation performances. Scenarios with minor regulation changes produced good short-term but poor long-term utilization. Stabilities were poor in continuing the current fishing mortality and fishing ban scenarios. Reliability in all scenarios after 30 years was smaller than in continuing the current fishing mortality; however, small differences among scenarios were observed. The simulation results indicated that multilateral assessment is needed to evaluate the management candidates. Uncertainty caused by recruitment variability mostly affected future population dynamics. The role of simulations in the production of effective scientific advice is discussed.  相似文献   

Structure of the intratesticular duct system for sperm emission in the starspotted smooth-hound Mustelus manazo     

Jeong-Chae Park  Jeong-Hoon Lee  Keita Kodama  Hiroshi Urushitani  Yasuhiko Ohta  Toshihiro Horiguchi 《Fisheries Science》2013,79(2):203-211

We conducted histological observation of male germ cells and reproductive organs of the starspotted smooth-hound Mustelus manazo in Tokyo Bay to reveal any abnormality in male reproductive traits, as part of a study to elucidate the factors causing recent fluctuation in abundance of the population. Spermatogenesis proceeded in spermatocysts from the germinal zone in the ventral part of the testis to the degenerative zone in the dorsal part, where the spermatozoa were conveyed into the ciliated lumina of the attached terminal branches of the intratesticular ducts. The intratesticular ducts were classified from their terminal ends into branch, stem, and collecting tubules. The ducts formed in the germinal zone and grew as the spermatocysts developed. An opening formed through the wall of each of the most mature spermatocysts into a branch tubule; bundles of spermatozoa were evacuated through this opening into the branch and then the stem tubule and subsequently into the collecting tubules in the rete testis and the efferent duct connected to the epididymis. Spermatocysts that were unable to emit sperm because of failure of adhesion to the branch tubules were disorganized in situ, as were their spermatozoa. The collapsed spermatocysts seem to be cleared by hemophagocytosis with lymphocytes and leukocytes, which may have been recruited from the epigonal organ. There were no specific abnormalities in the spermatogenesis or the morphological structure of testes, which suggested that an abnormality of male reproductive traits was not the major cause of the recent fluctuation in the population abundance of this species. Details of the intratesticular duct system for sperm emission to the epididymis are the first findings in elasmobranchs worldwide.  相似文献   

Dual effects of reversed winter–spring temperatures on year‐to‐year variation in the recruitment of chub mackerel (Scomber japonicus)     

Hitoshi Kaneko  Takeshi Okunishi  Takashi Seto  Hiroshi Kuroda  Sachihiko Itoh  Shinya Kouketsu  Daisuke Hasegawa 《Fisheries Oceanography》2019,28(2):212-227

To clarify the effects of temperature on the recruitment of chub mackerel (Scomber japonicus) in the North Pacific, we investigated the influence of winter surface temperature (WST) on spawners at the time of maturity around the spawning grounds and the influence of ambient spring temperature on larvae using estimated temperature (ET) obtained from particle tracking experiments. We found a significant positive correlation between ET approximately 10 days following hatching and the recruitment per spawning stock biomass (RPS) after 2000. The closer (more meandering) the Kuroshio Current (KC) axis was in relation to the spawning ground, the higher (lower) the spring surface temperature and the higher (lower) RPS was in the spawning ground. In contrast, WST inside KC near the maturity/spawning ground was significantly negatively correlated with RPS. A significant negative correlation between the temperatures in winter and spring was detected in the area after 2000, when the conditions of the Pacific decadal oscillation index and the stability of the Kuroshio Extension were synchronous, indicating that KC shifted northward during this time. The reversed temperature pattern was consistent with the winter–spring movement of KC axis in the offshore direction and was correlated with the winter–spring difference in the intensity of the Aleutian low. These results suggest that the annual variation in chub mackerel recruitment after 2000 was strongly affected by the combined effects of ambient temperature because of the reversal of conditions that occurred between winter and spring around the maturity/spawning ground, which was related to the KC path.  相似文献   

Application of the Tweedie distribution to zero-catch data in CPUE analysis   总被引：2，自引：0，他引：2  

Hiroshi Shono   《Fisheries Research》2008,93(1-2):154-162

We focus on the zero-catch problem of CPUE (catch per unit effort) standardization. Because the traditional CPUE model with a log-normal error structure cannot be applied in this case, three methods have often been utilized as follows:

(1) Ad hoc method adds a small constant value to all response variables.

(2) Catch model with a Poisson or negative-binomial (NB) error structure.

(3) Delta-type two-step method such as the delta-normal model (after estimating the ratio of zero-catch using a logit or probit model, a model such as CPUE log-normal or Catch-Poisson is applied to CPUE without zero-data).

However, there are some statistical problems with each of these methods.In this paper, we carried out the CPUE standardization mainly using the Tweedie distribution model based on the actual by-catch data (silky shark, Carcharhimus falciformis, in the North Pacific Ocean caught by Japanese training vessels) including many observations with zero-catch (>2/3rd) and tuna fishery data as a target (yellowfin tuna, Thunnus albacares, in the Indian Ocean caught by Japanese commercial vessels) where the ratio of zero-catch is not so high (<1/3rd). The Tweedie model is an extension of compound Poisson model derived from the stochastic process where the weight of the counted objects (i.e., number of fish) has a gamma distribution and has an advantage of handling the zero-catch data in a unified way.We also compared four candidate models, the Catch-NB model, ad hoc method, Delta-lognormal model (delta-type two-step method) and Tweedie distribution, through CPUE analyses of actual fishery data in terms of the statistical performance. Square error and Pearson's correlation coefficient were calculated based on the observed CPUE and the corresponding predicted CPUE using the n-fold cross-validation.As a result, the differences in the trend of CPUE between years and model performance between the ad hoc method and Tweedie model were found to be not so large in the example of yellowfin tuna (target species). However, the statistical performance of Tweedie distribution is rather better than Delta-lognormal model, the Catch-NB distribution and ad hoc method in the example of silky shark (by-catch species). Standardized CPUE year trend of ad hoc method was found to be quite different from that of the Tweedie distribution and other two models. Model performance of the Tweedie distribution is good judging from the 5-fold cross-validation using the fishery data if including many zero-catch data such as by-catch species.  相似文献