Extractives relating to heartwood color changes in sugi (<Emphasis Type="Italic">Cryptomeria japonica</Emphasis>) by a combination of smoke-heating and UV radiation exposure     

F.?Ishiguri  S.?Maruyama  K.?Takahashi  Z.?Abe  S.?Yokota  M.?Andoh  N.?YoshizawaEmail author 《Journal of Wood Science》2003,49(2):135-139

 Sugi green logs with red or black heartwood were smoke-heated, and the changes in the color of the heartwood after ultraviolet (UV) (λ = 365 nm) radiation exposure were then observed. After UV radiation exposure, the redness and yellowness increased in both the red and black heartwoods, whereas the brightness decreased. In the black heartwood, the resulting color turned from yellowish white to reddish brown. Reddening in black heartwood after exposure to a combination of smoke heating and UV radiation is thought to be due to a decrease in brightness and an increase in both redness and yellowness. However, the degree of change in heartwood color by UV radiation exposure was not greatly affected by smoke-heating treatments of various lengths. When methanol extracts were fractionated and exposed to UV radiation, the yellowness increased in the n-hexane-soluble portion and the redness increased in the acetone-soluble fractions from the n-hexane-insoluble portion. These results suggest that the n-hexane-soluble fraction contains the substances that allow heartwood color to change to yellow after UV radiation exposure, and the acetone-soluble-fraction from the n-hexane-insoluble portion contains the substances that allow it to change to red. Received: November 14, 2001 / Accepted: June 3, 2002 Acknowledgment This research was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science. This study was presented in part at the 51st Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001 Correspondence to:N. Yoshizawa  相似文献   

Tree size dependence of litter production, and above-ground net production in a young Hinoki (Chamaecyparis obtusa) stand     

Stephen Adu-Bredu  Taketo Yokota  Kazuharu Ogawa  Akio Hagihara 《Journal of Forest Research》1997,2(1):31-37

The study was carried out in a 9-year-old hinoki cypress (Chamaecyparis obtusa (Sieb. et Zucc.) Endl.), stand over a span of three years from July 1992 to June 1995, primarily to predict litter production from exteral tree dimensions by combining open-top clothtrap and clipping methods. Litter production was virtually concentrated in October and November. Stem cross-sectional area at the crown base was proved to be the reliable predictor of litter production, and that single regression model was evolved irrespective of year. The regression model had proportional constants of 2.696 × 10⁻² and 3.540 × 10⁻² kg cm⁻² year⁻¹ for leaf litter and total litter production, respectively. Utilizing the model, leaf litter production of the stand was assessed to be 5.04, 5.12, and 4.99, and total litter production to be 6.48, 6.58, and 6.40 Mg ha⁻¹ year⁻¹ for the first, second and third year, respectively. Biomass increment was 6.67, 7.80, and 7.70, tree mortality was 0.15, 0.13, and 0.41, and insect grazing was 0.09, 0.05, and 0.002 Mg ha⁻¹ year⁻¹ for the first, second and third year, respectively. Above-groud net production was therefore 13.39, 14.55, and 14.51, Mg ha⁻¹ year⁻¹, and biomass accumulation ratio (biomass/net production) was 1.86, 2.21, and 2.76 year for the first, second and third year, respectively. Considering data from earlier studies and the results of this study, biomass accumulation ratio,BAR (year), of hinoki stands was best related to above-ground biomass,y (Mg ha⁻¹), using the power function:BAR=0.112y ^0.936. Excluding seedling stands, leaf efficiency (above-ground net production per unit leaf mass) of hinoki stands was 0.91±0.02 (SE) Mg Mg⁻¹ year⁻¹, irrespective of stand biomass or age.  相似文献   

Investigation of relationships between cell and pulp properties in Eucalyptus by examination of within-tree property variations     

T. Ona  T. Sonoda  K. Ito  M. Shibata  Y. Tamai  Y. Kojima  J. Ohshima  S. Yokota  N. Yoshizawa 《Wood Science and Technology》2001,35(3):229-243

 Relationships between cell and pulp properties were investigated by examining the within-tree property variations in Eucalyptus camaldulensis and Eucalyptus globulus. Properties investigated included proportions of ray and axial parenchyma, thickness of cell walls and cell wall percentages. The characteristics of the ray and axial parenchyma (their proportions and wall thickness) were found to have a significant influence on all measured pulp properties, including paper strength properties. Multiple regression of pulp properties in relation to cell properties revealed that nearly all measured pulp properties were explained by cell properties at the 1% significance level. It was concluded, therefore, that all cell types are important for predicting pulp properties, and it is strongly recommended that tree breeding programs for Eucalyptus include the measurement of all cell types. Received 6 July 1999  相似文献   

Dependence of the aboveground respiration of hinoki cypress (Chamaecyparis obtusa) on tree size     

Yokota T  Ogawa K  Hagihara A 《Tree physiology》1994,14(5):467-479

Nighttime respiration was measured at monthly intervals over one year on the aboveground parts of five sample trees in an 8-year-old hinoki cypress (Chamaecyparis obtusa (Sieb. et Zucc.) Endl.) stand, by an enclosed standing-tree method. The respiration rate rose rapidly from early spring to a maximum in June, and decreased abruptly in July and then gradually toward autumn and winter. The seasonal change in the respiration rate was synchronized with stem volume increment rather than with monthly mean air temperature. The respiration rate, r, of individual trees increased with increasing tree dimensions, such as stem volume, v(S), and stem girth at the base of the live crown, G(B). The dependence of respiration rate on tree size was successfully represented by a power function. The r - v(S) dependence was rather stronger than the r - G(B) (2) dependence, especially toward the end of the growing season (from July to September). The observed respiration rate was almost the same as the respiration rate corrected for the monthly mean air temperature. The annual respiration of individual trees was directly proportional to their phytomass or to its increment. Although the annual respiration of individual trees decreased proportionally to the square root of the leaf mass, it decreased abruptly in the range close to the smallest sample tree. Combining the monthly relationship between respiration rate and stem volume with the tree size distribution in the stand, the stand aboveground annual respiration was estimated to be 20.4 Mg CO(2) ha(-1) year(-1) (= 12.5 Mg dry mass ha(-1) year(-1)) for an aboveground biomass of 17.4 Mg ha(-1) with an annual increment of 6.51 Mg ha(-1) year(-1), i.e., the stand aboveground annual respiration amounted to the equivalent of 72% of the biomass or to almost twice the biomass increment.  相似文献   

Maturity and reproduction of goneplacid crab <Emphasis Type="Italic">Carcinoplax vestita</Emphasis> (Decapoda,Brachyura) in Tokyo Bay     

Wataru Doi  Than Than Lwin  Masashi Yokota  Carlos Augusto Strüssmann  Seiichi Watanabe 《Fisheries Science》2007,73(2):331-340

ABSTRACT:     Sexual maturity, morphological sexual dimorphism, and reproduction of the goneplacid crab Carcinoplax vestita were investigated in Tokyo Bay, Japan, from November 2002 to October 2003. The puberty molt in males was evidenced by changes in the relative size of the chelipeds and merus of the walking legs, and was estimated to occur at a size range of 13.20–18.85 mm carapace length. Post-pubertal females were identified by the relative size of the abdomen and puberty was estimated to occur at a size range of 12.81–15.46 mm carapace length. Sexual dimorphism in C. vestita was observed in all features that showed secondary sexual characteristics. Monthly changes of gonad index in males, and of seminal receptacle index and occurrence of sperm plugs in females were synchronized, and indicated that mating was intense in spring. Ovaries began developing in March. Ovigerous females were found in all months except December, but were clearly more abundant between August and October. Fecundity ranged from 7800 to 57 000 mature oocytes per female per batch and was highly correlated with body size. The results suggest that some females may spawn more than one batch per year.  相似文献   

Interaction of grammistins with lipids and their antibacterial activity   总被引：1，自引：1，他引：1  

Hiroshi Yokota  Yuji Nagashima  Kazuo Shiomi 《Fisheries Science》2001,67(5):928-933

ABSTRACT: Grammistins are hemolytic and ichthyotoxic peptides in the skin secretion of soapfishes and are structurally characterized by their abundance in amphiphilic α-helicity. In the present study, their interaction with lipids and lipid vesicles as well as antibacterial activity were examined using four grammistins (Gs 1 and Gs 2 from Grammistes sexlineatus and Pp 1 and Pp 3 from Pogonoperca punctata ). The hemolytic activity of grammistins was inhibited by phospholipids but not by cholesterol. Moreover, grammistins released carboxyfluorescein entrapped within liposomes made of phosphatidylcholine. In contrast, grammistins were found to have antibacterial activity with a broad spectrum against nine species of bacteria, including both Gram-negative and Gram-positive groups. The potency of their antibacterial activity was not related to that of hemolytic activity, suggesting that grammistins bind to membrane phospholipids but lyse erythrocyte and bacterial membranes via different mechanisms. Conclusively, grammistins are new members of the family of cell non-selective membrane-lytic peptides with amphiphilic α-helices, being similar to pardaxins, which are secreted from the skin of soles, and to melittin, which is derived from bee venom.  相似文献   

Detection of an isoform of alpha(1)-antitrypsin in serum samples from foals with gastric ulcers     

Taharaguchi S  Nagano A  Okai K  Miyasho T  Kuwano M  Taniyama H  Yokota H 《The Veterinary record》2007,161(10):338-342

The objective of this study was to find serum indicators of gastric ulcers in foals. By using two-dimensional electrophoresis of serum proteins, three distinct spots were detected in samples from foals with gastric ulcers detected endoscopically. One of them appeared with high frequency and was identified by partial digestion with trypsin and subsequent nano-electrospray ionisation-tandem mass spectrometry (nanoesi-ms/ms) analysis as an alpha(1)-antitrypsin. Western blot analysis, using an antibody against human alpha(1)-antitrypsin, revealed at least two bands, of molecular weight 58 kDa and 55 kDa, in the sera. The 55 kDa band was detected in 44 of 47 serum samples from foals with gastric ulcers, but in only three of 22 serum samples from healthy foals.  相似文献   

Ellagitannins,flavonoids, and other phenolics in red raspberries and their contribution to antioxidant capacity and vasorelaxation properties     

Mullen W  McGinn J  Lean ME  MacLean MR  Gardner P  Duthie GG  Yokota T  Crozier A 《Journal of agricultural and food chemistry》2002,50(18):5191-5196

Analysis of extracts of Glen Ample raspberries (Rubus idaeus L.) by gradient, reverse phase HPLC with diode array and tandem mass spectrometry identified eleven anthocyanins, including cyanidin-3-sophoroside, cyanidin-3-(2(G)-glucosylrutinoside), cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-sophoroside, pelargonidin-3-(2(G)-glucosylrutinoside), and pelargonidin-3-glucoside. Significant quantities of an ellagitannin, sanguiin H-6, with an M(r) of 1870 were detected along with lower levels of a second ellagitannin, lambertianin C, which has an M(r) of 2804. Other phenolic compounds that were detected included trace levels of ellagic acid and its sugar conjugates along with one kaempferol- and four quercetin-based flavonol conjugates. Fractionation by preparative HPLC revealed that sanguiin H-6 was a major contributor to the antioxidant capacity of raspberries together with vitamin C and the anthocyanins. Vasodilation activity was restricted to fractions containing lambertianin C and sanguiin H-6.  相似文献   

Naidid worms (Oligochaeta, Naididae) in paddy soils as affected by the application of legume mulch and/or tillage practice     

Hiroshi Yokota  Nobuhiro Kaneko 《Biology and Fertility of Soils》2002,35(2):122-127

Reproduction, intrinsic rate of natural increase and population density of naidid worms were investigated in submerged paddy fields and the laboratory. No tillage plus legume-mulching increased the population density of naidid worms. Soil treatments with neither tillage nor legume mulch, and tillage practice alone, did not increase the number of worms. Dero dorsalis Ferronnière was dominant in soil of the no-tillage treatment. In laboratory experiments, legume-mulching with the proper amount of dissolved O₂ accelerated asexual reproduction of D. dorsalis through zooid budding. For the legume and aeration treatment, (N_i+1-N_i) N_i^-1 values (where N_i and N_i+1 are the populations at times t=i and t=i+1) were plotted against N_i+1. Utilizing this linear relation, this data fitted the logistic curve (r²=0.885, P<0.05). Based on the linear relation, the intrinsic rate of natural increase (r), carrying capacity (K), and doubling time (T) were calculated as 0.2125 day^-1, 12,666 m^-2, and 3.26 days, respectively. The amounts of legumes applied were highly correlated with the population of D. dorsalis, indicating that the weight of legume is a limiting factor with respect to carrying capacity. A literature review indicated a significant correlation (P<0.01) between intrinsic rate of natural increase and maximum body length of naidids with temperature conversion of the growth rate. Sexually mature worms were rarely found in submerged paddy fields. Sexual reproduction seems to be adopted in response to soil desiccation after paddy field drainage.  相似文献   

Analysis of strigolactones,germination stimulants for striga and orobanche,by high-performance liquid chromatography/tandem mass spectrometry     

Sato D  Awad AA  Chae SH  Yokota T  Sugimoto Y  Takeuchi Y  Yoneyama K 《Journal of agricultural and food chemistry》2003,51(5):1162-1168

A simple and rapid analytical method for strigolactones, germination stimulants for the root parasitic weeds witchweed (Striga spp.) and broomrape (Orobanche spp.), has been developed using high-performance liquid chromatography connected to tandem mass spectrometry (LC/MS/MS). The natural strigolactones (strigol, sorgolactone, orobanchol, and alectrol) were clearly separated and identified by LC/MS/MS. As low as 0.1 pg/microL of strigol and 0.5 pg/microL of sorgolactone could be quantified, whereas 1 pg/microL was needed for the quantification of orobanchol (S/N > 10). Using this method, it was found that red clover produces orobanchol and alectrol but not strigol. The roots of red clover seedlings were found to produce 13, 70, 58, and 65 pg of orobanchol/plant 1, 2, 3, and 4 weeks after germination, respectively.  相似文献