全文获取类型
收费全文 | 423篇 |
免费 | 58篇 |
专业分类
林业 | 19篇 |
农学 | 43篇 |
基础科学 | 5篇 |
85篇 | |
综合类 | 5篇 |
农作物 | 26篇 |
水产渔业 | 40篇 |
畜牧兽医 | 199篇 |
园艺 | 26篇 |
植物保护 | 33篇 |
出版年
2023年 | 4篇 |
2022年 | 12篇 |
2021年 | 20篇 |
2020年 | 29篇 |
2019年 | 29篇 |
2018年 | 29篇 |
2017年 | 23篇 |
2016年 | 28篇 |
2015年 | 26篇 |
2014年 | 25篇 |
2013年 | 31篇 |
2012年 | 35篇 |
2011年 | 30篇 |
2010年 | 24篇 |
2009年 | 22篇 |
2008年 | 18篇 |
2007年 | 23篇 |
2006年 | 17篇 |
2005年 | 17篇 |
2004年 | 10篇 |
2003年 | 10篇 |
2002年 | 5篇 |
2001年 | 2篇 |
2000年 | 5篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1983年 | 1篇 |
排序方式: 共有481条查询结果,搜索用时 15 毫秒
81.
Carlos Alberto Scapim Cleso Antônio Patto Pacheco Antônio Teixeira do Amaral Júnior Rafael Augusto Vieira Ronald José Barth Pinto Thiago Vincenzi Conrado 《Euphytica》2010,174(2):209-218
In plant breeding, correlations between the statistics of stability and adaptability of popcorn cultivars are not yet well
understood. Therefore, the objectives of the present experiment was to investigate the correlations between sdi2 \sigma_{\rm di}^{2} and bi \beta_{\rm i} from Eberhart and Russell, ωi from Wricke,
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} ,
\textS\texti(2) {\text{S}}_{\text{i}}^{(2)} and
\textS\texti(3) {\text{S}}_{\text{i}}^{(3)} from Huehn, Pi from Lin and Binns and the rank-sum from Kang, and indicate the most reliable method for selecting popcorn cultivars. These
statistics were estimated by data of crop yield from 19 Brazilian genotypes under 21 environments and popping expansion under
16 environments. The ωi,
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} ,
\textS\texti(2) {\text{S}}_{\text{i}}^{(2)} ,
\textS\texti(3) {\text{S}}_{\text{i}}^{(3)} and sdi2 \sigma_{\rm di}^{2} were positively and significantly correlated indicating that just one in these five statistics is sufficient for selecting
stable genotypes although they were not correlated with the means of crop yield and popping expansion. The bi \beta_{\rm i} was negatively and significantly correlated with Pi for crop yield indicating that the most adaptable genotypes tend to have the lowest estimates of Pi. Although Pi was not correlated with ωi,
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} ,
\textS\texti(2) {\text{S}}_{\text{i}}^{(2)} ,
\textS\texti(3) {\text{S}}_{\text{i}}^{(3)} , or sdi2 \sigma_{\rm di}^{2} statistics, it displayed positive correlation with the Index 1 (crop yield and popping expansion +
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} rank) and Index 2 (crop yield and popping expansion + Wi) indicating that superior popcorn genotypes are also stable. Finally, both Pi and the rank-sum are useful statistics in breeding programmes where crop yield, popping expansion and stability are essential
traits for selecting genotypes. 相似文献
82.
Diogenes Dezen Franciscus Antonius Maria Rijsewijk Thais Fumaco Teixeira Carine Lidiane Holz Samuel Paulo Cibulski Ana Cláudia Franco Odir Antonio Dellagostin Paulo M. Roehe 《Research in veterinary science》2010,88(3):436-440
Multiply-primed rolling-circle amplification (MPRCA) was used to amplify porcine circovirus type 2 (PCV2) genomes isolated from tissues of pigs with signs of post-weaning multisystemic wasting syndrome (PMWS). Two of the amplified PCV2 genomes were cloned in prokaryotic plasmids and sequenced. Both were nearly identical (1767 nt) except for one silent substitution in the region coding for the capsid protein (ORF2). In addition, they showed high nucleotide sequence similarity with PCV2 isolates from others countries (93–99%). To investigate whether the MPRCA amplified PCV2 genomes could be used to produce infectious virus, the cloned genomes were isolated from the plasmids, recircularized and used for transfection in PK-15 cells. This procedure led to the production of infectious virus to titres up to 105.55 TCID50/mL. It was concluded that MPRCA is a useful tool to amplify PCV2 genomes aiming at sequencing and virus isolation strategies, where particularly useful is the fact that it allows straightforward construction of PCV2 infectious clones from amplified genomes. However, it was less sensitive than PCR for diagnostic purposes. 相似文献
83.
Raquel M. Marques António Costa-e-Silva Artur P. Águas Luzia Teixeira Paula G. Ferreira 《Veterinary research communications》2010,34(8):659-668
Rabbit Haemorrhagic Disease (RHD) is a lethal infection caused by calicivirus that kills 90% of the infected adult rabbits
within 3 days. The calicivirus replicates in the liver and causes a fulminant hepatitis. Most studies on the pathology of
RHD have been focused on the fulminant liver disease. This may not be the only mechanism in the pathogenesis of RHD: calicivirus
infection may also induce leukopenia in the infected adult rabbits. We show now by flow cytometry analysis that the calicivirus
induces an early decrease in B and T cells, in both spleen and liver. The depletion of B and T cells was associated with apoptosis
labelled by annexin V. These changes occurred in rabbits before they showed enzymatic evidence of liver damage and persisted
after liver transaminase values were very high. We conclude that depletion of lymphocytes caused by the calicivirus infection
precedes or attends liver damage. The relative contribution of this lymphocyte depletion for the pathogenesis of the fatal
calicivirus infection of rabbits remains to be investigated. 相似文献
84.
Marta Lizandra do Reo Leal Emmanuel Veiga de Camargo Daniel Henrique Ross Marcelo Beltrão Molento Sonia Terezinha dos Anjos Lopes João Batista Teixeira da Rocha 《Veterinary research communications》2010,34(6):549-555
The objective of this study was to evaluate oxidative stress in lambs experimentally infected with Haemonchus contortus and supplemented with selenium and vitamin E. Twenty male Corriedale lambs were divided into four experimental groups with
five animals each: G1 consisted of animals infected and supplemented with 0.2 mg/kg of live weight (LW) sodium selenite by
intramuscular injection (IM); G2 consisted of animals infected with larvae and supplemented with 0.2 mg/kg LW sodium selenite
IM and 2000 IU per animal of Vitamin E IM; G3 consisted of animals infected with larvae and supplemented with 2000 IU per
animal of Vitamin E IM; and G4 consisted of animals infected with larvae. The animals were infected orally with 500 H. contortus larvae (L3) every 48 hours for 20 days. For biochemical analyses and eggs per grams of feces (EPG) evaluation, blood and
feces were both collected at zero (T0), 20 (T1), 40 (T2) and 60 (T3) days. The weight of the animals was also measured at
these times. Lower TBARS values were observed in the supplemented animals compared to the control group. The groups supplemented
with Selenium exhibited blood GSH-Px activity higher than that of non-supplemented animals. Supplementation with selenium
provided greater antioxidant protection against oxidative stress generated from experimental infection of lambs with H. contortus. 相似文献
85.
Renato G Credie Francisco J Teixeira Neto Tatiana H Ferreira Antônio JA Aguiar Fabio C Restitutti José E Corrente 《Veterinary anaesthesia and analgesia》2010,37(3):240-249
ObjectiveTo investigate the effects of methadone on the minimum alveolar concentration of isoflurane (ISOMAC) in dogs.Study designProspective, randomized cross-over experimental study.AnimalsSix adult mongrel dogs, four males and two females, weighing 22.8 ± 6.6 kg.MethodsAnimals were anesthetized with isoflurane and mechanically ventilated on three separate days, at least 1 week apart. Core temperature was maintained between 37.5 and 38.5 °C during ISOMAC determinations. On each study day, ISOMAC was determined using electrical stimulation of the antebrachium (50 V, 50 Hz, 10 mseconds) at 2.5 and 5 hours after intravenous injection of physiological saline (control) or one of two doses of methadone (0.5 or 1.0 mg kg?1).ResultsMean (±SD) ISOMAC in the control treatment was 1.19 ± 0.15% and 1.18 ± 0.15% at 2.5 and 5 hours, respectively. The 1.0 mg kg?1 dose of methadone reduced ISOMAC by 48% (2.5 hours) and by 30% (5 hours), whereas the 0.5 mg kg?1 dose caused smaller reductions in ISOMAC (35% and 15% reductions at 2.5 and 5 hours, respectively). Both doses of methadone decreased heart rate (HR), but the 1.0 mg kg?1 dose was associated with greater negative chronotropic actions (HR 37% lower than control) and mild metabolic acidosis at 2.5 hours. Mean arterial pressure increased in the MET1.0 treatment (13% higher than control) at 2.5 hours.Conclusions and clinical relevanceMethadone reduces ISOMAC in a dose-related fashion and this effect is lessened over time. Although the isoflurane sparing effect of the 0.5 mg kg?1 dose of methadone was smaller in comparison to the 1.0 mg kg?1 dose, the lower dose is recommended for clinical use because it results in less evidence of cardiovascular impairment. 相似文献
86.
A stable Leifsonia xyli subsp. xyli GFP‐tagged strain reveals a new colonization niche in sugarcane tissues 下载免费PDF全文
M. C. Quecine T. M. Silva G. Carvalho S. Saito M. Mondin N. S. Teixeira‐Silva L. E. A. Camargo C. B. Monteiro‐Vitorello 《Plant pathology》2016,65(1):154-162
Ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli (Lxx), is one of the most economically important diseases of sugarcane worldwide. Because knowledge on the interaction of Lxx with its host at the microscopic level is limited, the development of tools to monitor Lxx during the colonization process could shed new light on the processes that control disease development. In this investigation, a transformation protocol was optimized and a mutant Lxx strain engineered that stably expressed the gfp gene in sugarcane tissues. In vitro, the growth of the mutant did not differ from that of the wild type. Also, plants inoculated with both strains showed comparable growth and development when analysed 180 days after inoculation (dai). Fluorescence microscopy of roots, stalks, meristems and leaf tissues of Lxx‐GFP‐inoculated plants was performed at 180 dai. In the leaves, Lxx‐tagged cells were observed within the xylem vessels as has been described before but, in addition, they were found in a new niche within the host tissues, in the mesophyll and in the bundle sheath cells surrounding the vascular system. This finding indicates that Lxx is able to move from the xylem to the parenchyma of the leaf cells. This first report of an Lxx mutant expressing a heterologous gene revealed that colonization of sugarcane by this pathogen is not limited to the xylem vessels as commonly reported. 相似文献
87.
Magda Beretta Vicky Britto Tania Mascarenhas Tavares Sonilda Maria Teixeira da Silva Adelmo Lowe Pletsch 《Journal of Soils and Sediments》2014,14(7):1278-1286
Purpose
The Todos os Santos Bay is the largest bay in Brazil and receives drainage from various watersheds. For more than 450 years, it was the main destination for the domestic and hospital sewage from the city of Salvador, Bahia. With the growing concern regarding the presence of pharmaceutical and personal care products (PPCPs) in the environment, an investigation was undertaken to determine the presence and levels of some commonly used drugs (i.e., atenolol, caffeine, carbamazepine, diazepam, diclofenac, erythromycin, ibuprofen) and personal care products (i.e., galaxolide, tonalide), using sediments as an indicator of their presence in the water column.Material and methods
Surficial sediment samples from 17 stations located in the intertidal zone of the Todos os Santos Bay and infralittoral zone along the north coast of Salvador were tested for the presence of some PPCPs using LC-MS/MS (for drugs) and GC-MS/MS (for fragrances).Results and discussion
The PPCPs examined were present in all sediment samples at levels of parts per billion of dry sediment. The highest concentrations were found for the fragrances galaxolide (52.5 ng g?1) and tonalide (27.9 ng g?1), followed by caffeine (23.4 ng g?1) and pharmaceuticals ibuprofen (14.3 ng g?1), atenolol (9.84 ng g?1), carbamazepine (4.81 ng g?1), erythromycin (2.29 ng g?1), diclofenac (1.06 ng g?1), and diazepam (0.71 ng g?1).Conclusions
Pharmaceuticals were found to be ubiquitous in the sediments of the study areas. The texture of the sediment was an important factor in PPCPs fixation and deposition. The concentrations of all PPCPs had statistically significant positive correlations with the percentage of clay in the sediments. 相似文献88.
Batista JS Rodrigues CM García HA Bezerra FS Olinda RG Teixeira MM Soto-Blanco B 《Veterinary research》2011,42(1):63
ABSTRACT: Changes in cerebrospinal fluid (CSF) and anatomical and histopathological central nervous system (CNS) lesions were evaluated, and the presence of Trypanosoma vivax in CNS tissues was investigated through PCR. Twelve adult male goats were divided into three groups (G): G1, infected with T. vivax and evaluated during the acute phase; G2, infected goats evaluated during the chronic phase; and G3, consisting of non-infected goats. Each goat from G1 and G2 was infected with 1.25 × 105 trypomastigotes. Cerebrospinal fluid (CSF) analysis and investigation of T. vivax was performed at the 15th day post-infection (dpi) in G1 goats and on the fifth day after the manifestation of nervous system infection signs in G2 goats. All goats were necropsied, and CNS fragments from G1 and G2 goats were evaluated by PCR for the determination of T. vivax. Hyperthermia, anemia and parasitemia were observed from the fifth dpi for G1 and G2, with the highest parasitemia peak between the seventh and 21st dpi. Nervous system infection signs were observed in three G2 goats between the 30th and 35th dpi. CSF analysis revealed the presence of T. vivax for G2. Meningitis and meningoencephalitis were diagnosed in G2. PCR were positive for T. vivax in all the samples tested. In conclusion, T. vivax may reach the nervous tissue resulting in immune response from the host, which is the cause of progressive clinical and pathological manifestations of the CNS in experimentally infected goats. 相似文献
89.
Galiza GJ Garcia HA Assis AC Oliveira DM Pimentel LA Dantas AF Simões SV Teixeira MM Riet-Correa F 《Veterinary parasitology》2011,182(2-4):359-363
Here, we report an outbreak of Trypanosoma vivax-induced trypanosomosis in Brazilian hair sheep on a farm in Paraíba state, a non-endemic region in northeastern Brazilian. Of 306 total sheep, 240 showed clinical signs and 216 died. Clinical signs included anorexia, lethargy, anemia, rough hair coat, weight loss, submandibular edema, abortion, and in some cases, neurological signs such as head pressing, lateral recumbence, paddling movements and muscle tremors. T. vivax was identified by blood smear analysis and polymerase chain reaction (PCR). At necropsy, animals exhibited watery blood, pale tissue coloring, and the presence of liquid in the peritoneal cavity and pericardial sac. Histologically, nonsuppurative myocarditis and meningoencephalitis with areas of malacia were observed. After treatment, no parasites were detected by blood smear analysis or PCR. Cattle and buffalo that remained in the same pasture were also infected but presented with asymptomatic infections. Epidemiological data suggest that T. vivax was introduced to the farm and the susceptible flock by buffalos that were asymptomatic carriers of the infection; T. vivax was most likely transmitted by Tabanus spp. bites and also iatrogenically. 相似文献
90.
Fornazari F Teixeira CR da Silva RC Leiva M de Almeida SC Langoni H 《Veterinary parasitology》2011,179(1-3):238-241
Considering that Toxoplasma gondii is a parasite of global importance which affects several animal species including humans, the current study aimed to investigate the prevalence of antibodies against T. gondii among 72 white-eared opossums (Didelphis albiventris) from Botucatu Municipality (22°53'S 48°26'W), S?o Paulo State, Brazil. The investigation was carried out from January 2008 to December 2009, when the animals had their blood samples collected and subjected to the modified agglutination test (MAT); 12 specimens had brain samples bioassayed in mice. Seroprevalence was 5.5% (n=4) and bioassays were negative. Older animals had higher prevalence of antibodies against T. gondii. Opossums in closer contact with the urban environment are likely more exposed to T. gondii than animals from the sylvatic environment. 相似文献