Herbivory of an invasive slug in a model grassland community can be affected by earthworms and mycorrhizal fungi     

Raphaël Trouvé  Thomas Drapela  Thomas Frank  Franz Hadacek  Johann G. Zaller 《Biology and Fertility of Soils》2014,50(1):13-23

Invasion of non-native species is among the top threats for the biodiversity and functioning of native and agricultural ecosystems worldwide. We investigated whether the herbivory of the slug Arion vulgaris (formerly Arion lusitanicus; Gastropoda), that is listed among the 100 worst alien species in Europe, is affected by soil organisms commonly present in terrestrial ecosystems (i.e. earthworms—Annelida: Lumbricidae and arbuscular mycorrhizal fungi—AMF, Glomerales). We hypothesized that slug herbivory would be affected by soil organisms via altered plant nutrient availability and plant quality. In a greenhouse experiment, we created a simple plant community consisting of a grass, a forb, and a legume species and inoculated these systems with either two earthworm species and/or four AMF taxa. Slugs were introduced after plants were established. Earthworms significantly reduced total slug herbivory in AMF-inoculated plant communities (P?=?0.013). Across plant species, earthworms increased leaf total N and secondary metabolites, AMF decreased leaf thickness. Mycorrhizae induced a shift in slug feeding preference from non-legumes to legumes; the grass was generally avoided by slugs. AMF effects on legume herbivory can partly be explained by the AMF-induced increase in total N and decrease in C/N ratio; earthworm effects are less clear as no worm-induced alterations of legume plant chemistry were observed. The presence of earthworms increased average AMF colonization of plant roots by 140 % (P?<?0.001). Total shoot mass was significantly increased by AMF (P?<?0.001). These data suggest that the feeding behavior of this invasive slug is altered by a belowground control of plant chemical quality and community structure.  相似文献   

燃油温度和喷射压力对葵花籽油与柴油喷雾特性的影响     

何旭  刘海  曾威霖  余红东  Bohl Thomas  田国弘  李向荣  刘福水 《农业工程学报》2014,30(15):75-82

为探究纯植物油作为压燃内燃机替代燃料的雾化问题,利用纹影法对葵花籽纯植物油和普通柴油在不同试验条件下的喷雾特性进行了对比研究,分析了燃油温度和喷射压力对2种燃料喷雾贯穿距、喷雾锥角和空气卷吸作用的影响。研究结果表明:葵花籽油的喷雾贯穿距和喷雾锥角均随着喷射压力和燃油温度的升高而增大,与柴油的变化规律相似。温度从60℃升高到90℃,喷油压力为60 MPa时喷雾贯穿距和喷雾锥角的平均增幅分别为12.4%和9.6%,120 MPa时分别为5%和4.14%;喷油压力从60 MPa升高到120 MPa,温度为60℃时喷雾贯穿距和喷雾锥角的平均增幅分别为39.7%和16.7%,90℃时分别为30.6%和10.9%;当喷射压力、背景压力和燃油温度相同时,葵花籽油比柴油具有更大的贯穿距和更小的喷雾锥角,且喷雾贯穿距平均增幅为22.5%,喷雾锥角平均降幅为60.3%;升高燃油温度和升高喷射压力都会增强雾注的空气卷吸作用,但葵花籽油的空气卷吸作用要明显弱于普通柴油。研究结果可为纯植物油作为内燃机替代燃料的可行性研究提供参考。  相似文献   

Structure determination of 3-O-caffeoyl-epi-gamma-quinide, an orphan bitter lactone in roasted coffee     

Frank O  Blumberg S  Krümpel G  Hofmann T 《Journal of agricultural and food chemistry》2008,56(20):9581-9585

Recent investigations on the bitterness of coffee as well as 5- O-caffeoyl quinic acid roasting mixtures indicated the existence of another, yet unknown, bitter lactone besides the previously identified bitter compounds 5- O-caffeoyl- muco-gamma-quinide, 3- O-caffeoyl-gamma-quinide, 4- O-caffeoyl- muco-gamma-quinide, 5- O-caffeoyl- epi-delta-quinide, and 4- O-caffeoyl-gamma-quinide. In the present study, this orphan bitter lactone was isolated from the reaction products generated by dry heating of 5- O-caffeoylquinic acid model, and its structure was determined as the previously unreported 3- O-caffeoyl- epi-gamma-quinide by means of liquid chromatography-mass spectrometry (LC-MS) and one-/two-dimensional NMR experiments. The occurrence of this bitter lactone, exhibiting a low bitter recognition threshold of 58 micromol/L, in coffee beverages could be confirmed by LC-MS/MS (negative electrospray ionization) operating in the multiple reaction monitoring mode.  相似文献   

Antioxidant effectiveness of phenolic apple juice extracts and their gut fermentation products in the human colon carcinoma cell line caco-2     

Bellion P  Hofmann T  Pool-Zobel BL  Will F  Dietrich H  Knaup B  Richling E  Baum M  Eisenbrand G  Janzowski C 《Journal of agricultural and food chemistry》2008,56(15):6310-6317

Apples represent a major dietary source of antioxidative polyphenols. Their metabolic conversion by the gut microflora might generate products that protect the intestine against oxidative damage. We studied the antioxidant effectiveness of supernatants of fermented apple juice extracts (F-AEs, 6 and 24 h fermentation) and of selected phenolic degradation products, identified by HPLC-DAD-ESI-MS. Cell free antioxidant capacity of unfermented apple juice extracts (AEs) was decreased after fermentation by 30-50%. In the human colon carcinoma cell line Caco-2, F-AEs (containing <0.5% of original AE-phenolics) decreased the reactive oxygen species (ROS) level more efficiently than the F-blank (fermented without AE) but were less effective than the respective AEs. Similarly, antioxidant effectiveness of individual degradation products was lower compared to respective AE constituents. Glutathione level was slightly increased and oxidative DNA damage slightly decreased by fermented AE03, rich in quercetin glycosides. In conclusion, F-AEs/degradation products exhibit antioxidant activity in colon cells but to a lesser extent than the respective unfermented AEs/constituents.  相似文献   

The use of lysimeter data for the test of two soil–water balance models: A case study     

Martin Wegehenkel  Yongqian Zhang  Thomas Zenker  Heiko Diestel 《植物养料与土壤学杂志》2008,171(5):762-776

Two soil–water balance models were tested by a comparison of simulated with measured daily rates of actual evapotranspiration, soil water storage, groundwater recharge, and capillary rise. These rates were obtained from twelve weighable lysimeters with three different soils and two different lower boundary conditions for the time period from January 1, 1996 to December 31, 1998. In that period, grass vegetation was grown on all lysimeters. These lysimeters are located in Berlin‐Dahlem, Germany. One model calculated the soil water balance using the Richards equation. The other one used a capacitance approach. Both models used the same modified Penman formula for the estimation of potential evapotranspiration and the same simple empirical vegetation model for the calculation of transpiration, interception, and evaporation. The comparisons of simulated with measured model outputs were analyzed using the modeling‐efficiency index IA and the root mean squared error RMSE. At some lysimeters, the uncalibrated application of both models led to an underestimation of cumulative and annual rates of groundwater recharge and capillary rise, despite a good simulation quality in terms of IA and RMSE. A calibration of soil‐hydraulic and vegetation parameters such as maximum rooting depth resulted in a better fit between simulated and observed cumulative and annual rates of groundwater recharge and capillary rise, but in some cases also decreased the simulation quality of both models in terms of IA and RMSE. The results of this calibration indicated that, in addition to a precise determination of the soil water‐retention functions, vegetation parameters such as rooting depth should also be observed. Without such information, the rooting depth is a calibration parameter. However, in some cases, the uncalibrated application of both models also led to an acceptable fit between measured and simulated model outputs.  相似文献   

Orosensory-directed identification of astringent mouthfeel and bitter-tasting compounds in red wine     

Hufnagel JC  Hofmann T 《Journal of agricultural and food chemistry》2008,56(4):1376-1386

Application of sequential solvent extraction, followed by HPLC combined with the taste dilution analysis, enabled the localization of the most intense velvety astringent, drying, and puckering astringent, as well as bitter-tasting, compounds in red wine, respectively. Isolation of the taste components involving gel adsorption chromatography, ultrafiltration, and synthesis revealed the identification of 26 sensory-active nonvolatiles, among which several hydroxybenzoic acids, hydroxycinnamic acids, flavon-3-ol glycosides, and dihydroflavon-3-ol rhamnosides as well as a structurally undefined polymeric fraction (>5 kDa) were identified as the key astringent components. In contradiction to literature suggestions, flavan-3-ols were found to be not of major importance for astringency and bitter taste, respectively. Surprisingly, a series of hydroxybenzoic acid ethyl esters and hydroxycinnamic acid ethyl esters were identified as bitter compounds in wine. Taste qualities and taste threshold concentrations of the individual wine components were determined by means of a three-alternative forced-choice test and the half-mouth test, respectively.  相似文献   

Estimation of genetic variance components and heritabilities for cut-flower yield in gerbera using least squares and maximum likelihood methods     

James Harding  Hongzhan Huang  Thomas Byrne 《Euphytica》1996,88(1):55-60

Summary Genetic variance components and heritability were estimated for cut-flower yield of gerbera in the Davis population using ordinary least squares and maximum likelihood methods. The overall estimate of narrow-sense heritability is 0.33 based on least squares (LS) and 0.31 based on maximum likelihood (ML). The results of the study indicate that (1) ML and LS provide very similar results if sample size is large enough, suggesting both are useful for plant breeding programs; (2) about one third of the variation in gerbera cut-flower yield is additive, implying selection in cut-flower will be successful; and (3) although additive variation gradually decreased, heritability remained near 0.27 suggesting there is still potential variation in the population for further selection.  相似文献   

Classification of Azolla spp., section Azolla     

William J. Zimmerman  Thomas A. Lumpkin  Iwao Watanabe 《Euphytica》1989,43(3):223-232

Summary Azolla accessions (section Azolla) from the germplasm collections of the International Rice Research Institute and Washington State University were fingerprinted and classified by enzyme electrophoresis and leaf trichome morphology. A. filiculoides was enzymatically distinctive and also reliably identified by its prominent one-celled trichomes. Neotropical accessions labelled as A. filiculoides proved to be members of other species. Two groups of isolates were designated A. rubra, but those from Japan were identified as A. filiculoides. The A. rubra of Australia-New Zealand was biochemically unique and possessed less protuberant trichomes than A. filiculoides. A. microphylla, A. mexicana, and A. caroliniana were phenetically similar, but a. microphylla was identifiable from the others in the banding patterns of certain enzymes. A. mexicana and A. caroliniana were closely related enzymatically. The two-celled leaf trichomes of these three species were similar in size and shape.  相似文献   

Micro-scaled high-throughput digestion of plant tissue samples for multi-elemental analysis     

Thomas H Hansen  Kristian H Laursen  Daniel P Persson  Pai Pedas  Søren Husted  Jan K Schjoerring 《Plant methods》2009,5(1):12-11

Background  

Quantitative multi-elemental analysis by inductively coupled plasma (ICP) spectrometry depends on a complete digestion of solid samples. However, fast and thorough sample digestion is a challenging analytical task which constitutes a bottleneck in modern multi-elemental analysis. Additional obstacles may be that sample quantities are limited and elemental concentrations low. In such cases, digestion in small volumes with minimum dilution and contamination is required in order to obtain high accuracy data.  相似文献   

Determination of growth-stage-specific crop coefficients (Kc) of cotton and wheat     

Jonghan Ko  Giovanni Piccinni  Thomas Marek 《Agricultural Water Management》2009,96(12):1691-1697

Development of crop coefficient (Kc), the ratio of crop evapotranspiration (ETc) to reference evapotranspiration (ETo), can enhance ETc estimates in relation to specific crop phenological development. This research was conducted to determine growth-stage-specific Kc and crop water use for cotton (Gossypium hirsutum) and wheat (Triticum aestivum) at the Texas AgriLife Research field at Uvalde, TX, USA from 2005 to 2008. Weighing lysimeters were used to measure crop water use and local weather data were used to determine the reference evapotranspiration (ETo). Seven lysimeters, weighing about 14 Mg, consisted of undisturbed 1.5 m × 2.0 m × 2.2 m deep soil monoliths. Six lysimeters were located in the center of a 1-ha field beneath a linear-move sprinkler system equipped with low energy precision application (LEPA) and a seventh lysimeter was established to measure reference grass ETo. Crop water requirements, Kc determination, and comparison to existing FAO Kc values were determined over a 2-year period on cotton and a 3-year period on wheat. Seasonal total amounts of crop water use ranged from 689 to 830 mm for cotton and from 483 to 505 mm for wheat. The Kc values determined over the growing seasons varied from 0.2 to 1.5 for cotton and 0.1 to 1.7 for wheat. Some of the values corresponded and some did not correspond to those from FAO-56 and from the Texas High Plains and elsewhere in other states. We assume that the development of regionally based and growth-stage-specific Kc helps in irrigation management and provides precise water applications for this region.  相似文献