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51.
Agroforestry systems have the potential to increase sequestration of atmospheric carbon dioxide (CO2) as soil organic carbon (SOC) because of the increased rates of organic matter addition and retention. However, few studies have characterized the relative stability of sequestered SOC in soil. We characterized SOC storage in aggregate size and chemical stability classes to estimate the relative stability of SOC pools after the addition of Leucaena-KX2 pruning residues (mulch) from 2006 to 2008 in a shaded coffee agroforestry system in Hawaii. Soil samples were separated by microaggregate isolation, density flotation and dispersion, and acid hydrolysis, resulting in five distinct fractions that differed in relative stability: coarse particulate organic matter (POM), fine POM, microaggregate-protected POM, silt + clay hydrolyzable soil organic matter (SOM), and silt + clay non-hydrolyzable SOM. With mulch addition, the fine POM fraction increased. There was also a shift in the proportion of SOC to more stable silt + clay fractions. In the absence of mulch there was no significant change in SOC fractions. Given that the turnover time of SOC in silt + clay fractions is on the order of decades to centuries, the potential benefits of active shade management and mulching compensate for the loss of C sequestration in tree biomass from pollarding.  相似文献   
52.
Application of a hydrological model to three Canadian watersheds forms part of a verification package to test its accuracy and portability. Agreement of model results with observed data on hydrograph, groundwater flow and snowpack is reasonably good. The model results are consistent with the observed pH and with many of the episodic events that have occurred in these acidified watersheds. Contrasts have also been made on the different calibrated coefficients at several locations in these watersheds. An attempt to relate them to the geology and soil characteristics at the site has led to realistic estimation of the soil contact times.  相似文献   
53.
Mercury concentrations in largemouth bass (Micropterus salmoides) bass were compared among five reservoirs in South Carolina. Three of these reservoirs (Lake Russell, Lake Thurmond, and Lake Marion) are accessible to the public and two (L-Lake and Par Pond) are located on the U.S. Department of Energy's Savannah River Site (SRS), which is closed to public access. Age-adjusted mercury concentrations were significantly higher in SRS bass compared to the offsite reservoirs. In addition, mercury concentrations were significantly higher in bass from Par Pond compared to L-Lake and in bass from Lakes Russell and Thurmond compared to Lake Marion. No mercury concentrations in excess of the U.S. Food and Drug Administration action level (1.0 mg?kg?1) were found in any bass from the public-accessible reservoirs. However, the majority of fish from these reservoirs had mercury concentrations that fall into or exceed the U.S. Environmental Protection Agency consumption category of “no more than one per week". In addition, most fish from these reservoirs had mercury levels in excess of those believed to be detrimental to sensitive avian species.  相似文献   
54.
Effects of 12 years of 4 tillage systems, were studied on soil bulk density, infiltration rate, penetration resistance, structural stability of aggregates, root length density, moisture release characteristics and grain yield. The objective of this study was to assess long-term effects of tillage methods and guided traffic on soil properties and crop response on a heavy-textured poorly drained soil. Tillage systems included: (A) continuous no-till for 12 years; (B) no-till for 10 years followed by plow-till for 2 years; (C) plow-till for 10 years followed by no-till for 2 years; (D) continuous plow-till for 12 years. Wheel tracks had significant effects on soil physical properties. Soil in the traffic zone (TZ) in no-till treatment had higher bulk density and penetration resistance (PR) for the upper 0–30-cm layer than plow-till treatment. The PR for the surface layer in TZ was 25–46% more than in the row zone (RZ). Mean soil bulk density in the TZ of no-till plots was about 12% more than in the RZ (1.53 vs. 1.36 g cm−3). Changeover from no-till to plow-till decreased PR in RZ and TZ by 50–60% while that from plow-till to no-till increased PR by 10–20%. Similar effects were observed in percent aggregation and the mean weight diameter. In no-till treatments both initial and equilibrium infiltration rates were significantly lower in TZ than RZ (27.5 vs. 6.8 cm h−1 initial and 10.0 vs. 1.5 cm h−1 final). There were more macropores (> 2 mm) for the TZ in no-till compared with the plow-till treatments. Fine or micropores were comparatively more numerous in the sub-soil of no-till than plow-till treatments. The median aggregate size (D50) was 6.2, 4.2, 4.8 and 3.5 mm for Treatments A, B, C and D, respectively. Root length density of corn in plow-till plots was significantly more than that in no-till plots for the top 0–20-cm layer. Corn and soybean yields were greater in plow-till than no-till treatments. Grain yields in Treatment D were greater than those in Treatment A by 10% for corn and by 6% for soybean.  相似文献   
55.
Solar tracking has been described in several agronomic and wild species, yet variation within a given species has not been reported. To address that issue, photoperiod leaflet reorientation in response to vectorial light was measured in three alfalfa ( Medicago sativa L.) germplasm pools selected from the cultivar Ladak 65. Dark-leaf germplasm responded to all photosynthetic photon flux densities (PPFD) (100 to 700 μmol/m2/s) with no detectable lag period. Following a 24-h dark treatment only high PPFD (600 to 700μmol/m2/s) elicited reorientation. Pale-leaf germplasm responded to intermediate (300 to 400 μmol/m2/s) and high PPFD in a similar manner; however, the low (100-125μmol/m2/s) PPFD treatment had little effect on reorientation. The 24-h dark treatment decreased reorientation at all PPFD. Small-leaf germplasm responded equally to all PPFD and was not affected by the 24-h dark treatment. The results suggest that available energy levels may influence the reorientation response. Carbonyl cyanide m -chlorophenylhydrazone, an inhibitor of photophosphorylation, and 3-(3,4-dichlorophenyl)-1,1-dimethylurea, an inhibitor of non-cyclic photosynthetic electron transport, reduced reorientation only in the pale-leaf type. These results suggest that stored energy reserves were adequate to maintain leaflet reorientation in the absence of photosynthesis in all but the pale-leaf germplasm. In that germplasm photosynthetically generated energy may be required to support reorientation.  相似文献   
56.
Blood collected in either sodium heparin or disodium edetate vacutainers from febrile goats infected with 4 isolates of Cowdria ruminantium and cryopreserved with 10% dimethyl sulphoxide at -70 degrees C and -196 degrees C was an effective stabilate to initiate heartwater infections in goats. A homogenized pool of whole Amblyomma variegatum ticks in Snyder's buffer, maintained at -196 degrees C, was used to infect a goat with C. ruminantium. Liver and spleen collected from Swiss mice infected with the Kwanyanga isolate of C. ruminantium were homogenized in Snyder's buffer, maintained at -196 degrees C and were used to initiate infections in mice. Fresh blood collected from febrile goats and maintained at 4 degrees C for as long as 72 h was infectious to mice. Neutrophils separated from blood of C. ruminantium infected goats and maintained in modified RPMI medium at 37 degrees C for 68 h were infectious for a goat. Similarly neutrophils from a 2nd infected goat maintained for 96 h at 37 degrees C were infectious for mice.  相似文献   
57.
It is well established that the survival of newborn calves is dependent on their absorbing adequate amounts of maternal immunoglobulin from colostrum. The level of immunity is influenced by the time of feeding after birth and the quantity and quality of the available colostrum. Several factors affect either the quantity or quality of colostrum. The yield of colostrum varies with breed but perhaps more significantly with age, heifers giving much less colostrum than cows. Poor nutrition during winter months causes a marked drop in colostral quantity and often in the early spring a large number of suckler cows give insufficient colostrum to protect their calves from disease. Colostral quality in terms of immunoglobulin concentration is unaffected by yield but varies considerably between individual cows.Much of the present evidence is equivocal and there are many subjects for further research. A detailed study should be carried out, particularly in beef cattle, to establish which breed has the highest yield of colostrum. More research should be directed to establish the minimum plane of nutrition necessary to maintain colostrum quantity and quality.
Kurzfassung Die Tatsache, dass die Lebensfähigkeit neugeborener Kälber von der Aufnahme von genügend mütterlichen Immunoglobinen aus dem Kolostrum abhängt, ist wohlbekannt. Das Immunitätsniveau wird von der Säugezeit nach der Geburt und von der Menge und Qualität des verfügbaren Kolostrums beeinflusst. Mehrere Faktoren wirken entweder auf die Menge oder die Qualität des Kolostrums ein. Die Menge variiert mit der Rasse, aber vielleicht noch wichtiger mit dem Alter, da Färsen viel weniger Kolostrum geben als Kühe. Unzureichende Fütterung während der Wintermonate verursacht einen starken Fall der Kolostralleistung; in den ersten Frühjahrstagen gibt eine grosse Anzahl von Ammenkühen oft nicht genug Kolostrum, um die Widerstandsfähigkeit ihrer Kälber gegen Krankheiten zu sichern. Im Sinne von Immunoglobulinkonzentration wird die Qualität des Kolostrums nicht von seiner Menge beeinflusst, doch variiert sie beträchtlich zwischen einzelnen Kühen.Viele gegenwärtige Resultate sind zweideutig und viele Probleme müssen noch bearbeitet werden. Es bedarf auch einer vertieften Studie, insbesondere bei Fleischrindern, um festzustellen, welche Rasse die höchste Kolostralleistung hat. Man müsste auch Untersuchungen anstellen, um das minimale Fütterungsniveau fur die Erhaltung der Qualität und der Menge des Kolostrums zu ermitteln.

Resume Le fait que la survie des veaux nouveau-nés dépend de leur pouvoir d'absorber une quantité adéquate d'immoglobulines maternelles par le colostrum, est bien établi. Le niveau d'immunité est influencé par le temps de la tétée après la naissance, et par la quantité ainsi que la qualité du colostrum disponible. La production de colostrum varie, selon la race, mais peut-être l'âge est plus significatif, puisque les génisses donnent beaucoup moins de colostrum que les vaches. Une alimentation de qualité inférieure pendant les mois d'hiver cause une baisse importante de la quantité de colostrum, et souvent, beaucoup de vaches nourrices donnent trop peu de colostrum, aux premiers jours du printemps, pour protéger leurs veaux contre les maladies. La qualité du colostrum n'est pas effectée par le niveau de production, en termes de concentration d'immunoglobulines, mais elle varie considérablement entre les individus.L'évidence actuelle est équivoque, et beaucoup de sujets demandent encore des recherches. Il faudrait une étude approfondie pour établir la race à production de colostrum la plus élevée, surtout chez les bovins de boucherie. Il faudrait aussi des recherches plus poussées pour établir le niveau minimum nutritionnel nécéssaire pour maintenir la quantité et la qualité du colostrum.

Riassunto E'un fatto ben accertato che la sopravvivenza dei vitelli neonati dipende dall'assorbimento di una adeguata quantità di immunoglobuline materne attraverso il colostro. Il livello immunitario è a sua volta influenzato dal momento in cui si ha la poppata dopo la nascita e dalla quantità e qualità del colostro fornito. Svariati fattori influiscono sia sulla quantità che sulla qualità del colostro. La produzione di questo varia infatti a seconda della razza, ma varia forse anche in misura maggiore in rapporto all' età; le giovenche, infatti, producono molto meno colostro delle bovine di età superiore. Un apporto alimentare insufficiente durante i mesi invernali causa una notevole caduta della quantità di colostro e spasso, all'inizio della primavera, un gran numero di bovine in lattazione ne producono in quantità non sufficiente ai fini della protezione dei vitelli contro le malattie. La qualità del colostro in termini di concentrazione di immunoglobuline non viene influenzata dal livello di produzione, ma varia in modo considerevole da bovina a bovina.Gran parte dei dati oggi disponibili è oggetto di dubbio, ed esistono molti campi da sottoporre ad ulteriori indagini. Sarebbe necessario eseguire uno studio dettagliato, specie nelle bovine da carne, per stabilire quale razza ha la più alta produzione di colostro. Altre ricerche andrebbero indirizzate a stabilire il valore minimo alimentare necessario per il mantenimento del livello quanti-qualitativo del colostro.
  相似文献   
58.
59.
The development of Cowdria ruminantium in neutrophils   总被引:4,自引:0,他引:4  
The sequential development of C. ruminantium (Kwanyanga and Kümm isolates) was followed in caprine leukocyte cultures by light microscopy, direct immunofluorescent microscopy (DFA), indirect immunoflourescent microscopy (IFA) and transmission electron microscopy (TEM). During the febrile response, one to several small cocci, large ring forms or rods were observed in neutrophils in blood smears and cytopreparations of neutrophil fractions using Diff Quik stain, Giemsa stain, DFA and TEM. One to several C. ruminantium colonies were seen in up to 35% of neutrophils maintained in vitro for 18 h to 5 days. The organisms were located in neutrophil phagosomes by TEM and were enveloped by two trilamellar unit membranes. Initially, C. ruminantium was tightly enclosed within phagosomes. At 20 h of incubation, organisms were frequently observed undergoing binary fission within enlarged phagosomal vacuoles. At later time periods, neutrophils harboured fully formed colonies (morula) containing numerous organisms. An occasional C. ruminantium-infected macrophage (Kümm isolate), and an occasional infected eosinophil (Kümm and Kwanyanga isolate) were found.  相似文献   
60.
Dermacentor variabilis were infected as nymphs with Anaplasma marginale by allowing the ticks to feed on a single infected donor calf. Two weeks after molting to the adult stage, the ticks were allotted into 1 of 3 groups and were allowed to overwinter at room temperature (25 C) in the laboratory (group 1), cold storage (4.5 C) in the laboratory (group 2), or outdoors in leaf litter (group 3). Persistence of A marginale was assessed by determining density of colonies (number of colonies/0.1 mm2 of gut tissue examined) in tick gut specimens at 3, 5, 7, 9, and 12 months after molting to the adult stage. Colonies of A marginale were found in all groups at every density evaluation period. Highest colony densities were observed uniformly in specimens collected at month 7 (May); densities decreased at month 9 and were lowest at month 12. Statistical analysis indicated that ticks subjected to cold storage and to outdoor conditions had similar colony densities of A marginale; the density curve in these 2 groups indicated significant quadratic effects over time, with peak densities in May. Mean colony density in ticks kept at room temperature fit a different quadratic equation. The morphologic data indicated that A marginale overwinters in Dermacentor variabilis, and that increasing numbers of organisms are found from January to May.  相似文献   
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