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41.
Sixty-eight cases of suppurative, ulcerative endometritis associated with Bovine Herpesvirus-4 (BHV-4) in postparturient dairy cows (62 Holsteins and 6 Jerseys, mean age 4.2 years) were confirmed by a combination of histopathology, fluorescent antibody assays, electron microscopic evaluation of uterus, and polymerase chain reaction (PCR). All cases occurred in the 3- to 28-day postpartum period, and histologic lesions among various cows were consistent when compared with postpartum interval. The endometrial lining epithelium was necrotic and ulcerated from 3 to 7 days postpartum, with only mild inflammation in the lamina propria and submucosa. From 1 to 4 weeks postpartum, the ulcers were confluent to diffuse. Epithelium was replaced by fibrinonecrotic, suppurative mats, resulting in severe bacterial pyometra by day 24. Seroprevalence to BHV-4 in one dairy with a history of 18 mortality cases was 36% (107 of 296). In a random sample of 8 cows from this herd, none had serologic titers in blood sampled 2 weeks prepartum, but 3 of 8 seroconverted with significant titers of 1:8 to 1:16 at 2 weeks postpartum. By 10 weeks postpartum, all 8 cows returned to negative serologic status. Two of 6 cats from the premises also had positive titers. Random serum samples taken from 480 dairy cattle at sale barns indicated 76 (16%) were positive by serum neutralization. Clinical signs, postparturient timing, and histologic lesions were very similar to those previously reported in Belgium with BHV-4. But sequence analysis of PCR products of the glycoprotein B region of 4 separate field isolates of endometriotropic BHV-4 suggests these field isolates were more closely related to the North American nonvirulent strain DN-599 than to the endometriotropic European strain V.  相似文献   
42.
Necropsy records for 102 Attwater's prairie chickens (Tympanuchus cupido attwateri) that had been captive-reared and had died at the San Antonio Zoo over a 4-yr period were evaluated to determine the causes of mortality. Gross necropsy, histopathology, and bacterial culture findings are summarized. Mortality was highest in young birds (<6 mo) and embryos. Husbandry- and gastrointestinal-related diseases were the most common causes of mortality.  相似文献   
43.
It is increasingly important to be able to monitor and maintain the quality of estuaries for overwintering shorebirds. Bird numbers alone are not sufficient to indicate quality nor, as recent research shows, can it be assumed that site quality is being maintained simply because there is enough food to meet the birds’ physiological demands; i.e., the amount of food available per bird in autumn needs to be greater than the amount eaten. But how much greater? We used a simple individuals-based and behaviour-based model to explore the factors that affect the relationship between overwinter mortality and the amount of food available per bird in autumn (the food:bird ratio). The aims were to explore how the natural history characteristics of a shorebird species affected the shape of this relationship, in order to identify characteristics of the system that should be included in any monitoring programme of site quality. In all cases there was a minimum threshold food:bird ratio above which mortality did not vary and below which mortality increased steadily. The amount of food per bird required at the threshold varied from one to six times the amount actually consumed by each bird during winter, depending on the amount of realism included in the model. The minimum threshold ratio was most strongly influenced by the shape of the relationship between intake rate and prey density - the functional response - and, when interference competition occurred, by the distribution of the birds’ main food supply. Simulations with realistic spatial distributions of prey predicted that the minimum required amount of food per bird would be between two and five times the amount actually consumed. The threshold approach could provide a simple method of monitoring estuary quality based on food supply and bird numbers. It also provides a potentially clearly-defined and unambiguous measure of the carrying capacity of a site. If applied to a sufficient number of real estuaries, general guidelines for maintaining estuary quality based on estuary characteristics, food supply and bird species might be established.  相似文献   
44.
Bioavailability of dietary folate might be impaired by the polyglutamate chain to which approximately 70% of dietary folates are bound. This chain must be removed enzymatically in the intestine before folate is absorbed as a monoglutamate. To increase formation of monoglutamate folate in vegetables, the vegetables were subjected to various processing treatments. Treatments included freezing (-18 degrees C, 16 h) and thawing (4 degrees C, 24 h) and hydrostatic high-pressure treatment (200 MPa, 5 min). Both freezing/thawing and high-pressure treatment increased the proportion of folate in the monoglutamate form in leeks, cauliflower, and green beans 2-3-fold. However, loss of total folate after these treatments was >55%. It is concluded that conversion of folate polyglutamate to the monoglutamate form in vegetables is possible by certain processing treatments. Potentially this could lead to vegetables with higher folate bioavailability. However, to prevent folate loss into processing water, processing in a closed system should be applied.  相似文献   
45.
The preservation, age, and stratigraphic relation of an in situ ashfall layer with an underlying desert pavement in Arena Valley, southern Victoria Land, indicate that a cold-desert climate has persisted in Arena Valley during the past 4.3 million years. These data indicate that the present East Antarctic Ice Sheet has endured for this time and that average temperatures during the Pliocene in Arena Valley were no greater than 3 degrees C above present values. One implication is that the collapse of the East Antarctic Ice Sheet due to greenhouse warming is unlikely, even if global atmospheric temperatures rise to levels last experienced during mid-Pliocene times.  相似文献   
46.
REPORTS     
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47.
At least 16 fragments were detected in images of comet C/1999 S4 (LINEAR) taken on 5 August 2000 with the Hubble Space Telescope (HST) and on 6 August with the Very Large Telescope (VLT). Photometric analysis of the fragments indicates that the largest ones have effective spherical diameters of about 100 meters, which implies that the total mass in the observed fragments was about 2 x 10(9) kilograms. The comet's dust tail, which was the most prominent optical feature in August, was produced during a major fragmentation event, whose activity peaked on UT 22.8 +/- 0.2 July 2000. The mass of small particles (diameters less than about 230 micrometers) in the tail was about 4 x 10(8) kilograms, which is comparable to the mass contained in a large fragment and to the total mass lost from water sublimation after 21 July 2000 (about 3 x 10(8) kilograms). HST spectroscopic observations during 5 and 6 July 2000 demonstrate that the nucleus contained little carbon monoxide ice (ratio of carbon monoxide to water is less than or equal to 0.4%), which suggests that this volatile species did not play a role in the fragmentation of C/1999 S4 (LINEAR).  相似文献   
48.
M J West 《Science (New York, N.Y.)》1967,157(796):1584-1585
Interactions among female paper wasps of newly founded colonies suggest that dominance relations assign social (reproductive) roles to siblings in a way advantageous to both dominants and subordinates. In various social animals dominance relations may have been an important prerequisite for the evolution of a division of labor between reproductive and nonreproductive (or less reproductive) adults.  相似文献   
49.
Studies of the spectra of cyclopolysilanes have led to several significant conclusions. These molecules possess both easily ionized Si-Si bonding electrons and low energy delocalized antibonding orbitals, and can therefore serve either as electron donors or acceptors. This model explains the similarity in properties between cyclopolysilanes and aromatic hydrocarbons. Further investigations of cyclopolysilanes seem likely to provide the key to understanding of controversial questions of bonding in metalloid compounds even as studies of their carbon analogs, the cyclic and cage hydrocarbons, have been crucial to present knowledge of organic stereochemistry and reaction mechanisms. The reactions of cyclopolysilanes are not only interesting in themselves, but have opened the way to the synthesis of complex polysilanes and thus to whole new areas of study. Improved methods of synthesis and isolation are needed, but the number and kinds of compounds that can be prepared seem almost limitless. Perhaps a polymetal chemistry comparable in breadth and variety to carbon chemistry is now developing.  相似文献   
50.
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