脱脂乳发酵体系中乳酸菌的高效离子交换色谱分析     

张天亮  林娟  刘树滔  饶平凡 《乳业科学与技术》2007,29(3):119-121

应用高效离子交换色谱对脱脂乳发酵体系中的嗜热链球菌和保加利亚乳杆菌进行色谱表征。结果表明，单菌培养过程中保加利亚乳杆菌菌体形态的变化在其相应色谱行为上得到了体现，二者的色谱峰有比较好的稳定性。两种菌的峰面积与菌数之间呈现出良好的线性关系，可决系数R^2分别为0．9957和0．9916。因此本方法的建立，可用于酸奶发酵过程中乳酸菌的分析与表征，有望为乳酸菌混合物的研究提供一种快捷的新方法。  相似文献   

东乡野生稻原产地蜘蛛群落结构动态研究          下载免费PDF全文

袁凤辉  陈连水  范志刚  颜亨梅  饶军 《植物保护》2007,33(6):103-107

调查表明,东乡野生稻原产保护地蜘蛛群落优势科为狼蛛科、跳蛛科、管巢蛛科、球蛛科、肖蛸科,相对丰度分别为0.5010、0.1529、0.0785、0.0657、0.0559、0.0399、0.0392,其丰盛度总和达0.8540,构成了蜘蛛群落的主要成分;巨蟹蛛科为稀有科。优势种为拟水狼蛛、沟渠豹蛛、中华狼蛛、横窝希蛛、黄斑纯蛛、白纹舞蛛、微菱头蛛、柔弱锯螯蛛,其相对丰盛度分别为0.1802、0.1209、0.0833、0.0589、0.0484、0.0445、0.0417、0.0413,丰盛度总和达0.6192,埃比熊蛛等为稀有种。调查还表明,不同时期的物种丰富度变化不明显,但在6～7月间,群落中物种丰富度相对较高,各物种种群发生数量大,分布相对均匀,优势种不突出,群落结构较合理,均匀度和多样性指数较高。  相似文献   

Defective Movement of Viruses in the Family Bromoviridae Is Differentially Complemented in Nicotiana benthamiana Expressing Tobamovirus or Dianthovirus Movement Proteins     

Rao AL  Cooper B  Deom CM 《Phytopathology》1998,88(7):666-672

ABSTRACT Taxonomically distinct tobacco mosaic tobamovirus (TMV), red clover necrotic mosaic dianthovirus (RCNMV), cucumber mosaic cucumovirus (CMV), brome mosaic bromovirus (BMV), and cowpea chlorotic mottle bromovirus (CCMV) exhibit differences in their host range. Each of these viruses encodes a functionally similar nonstructural movement protein (MP) that is essential for cell-to-cell movement of a progeny virus. Despite the lack of significant amino acid identity among the MPs of CMV, TMV, and RCNMV, movement-defective CMV (CMVFnyDeltaMP-DeltaKPN) was able to move locally and systemically in transgenic Nicotiana benthamiana expressing either TMV MP (NB-TMV-MP(+)) or RCNMV MP (NB-RCNMV-MP(+)). These observations contrast with those of previous studies in which transgenic N. tabacum cv. Xanthi plants expressing TMV MP supported only the cell-to-cell movement of CMVFnyDeltaMP-DeltaKPN. To verify whether similar complementation could be observed for movement-defective bromoviruses, NB-TMV-MP(+) and NB-RCNMV-MP(+) plants were inoculated independently with movement-defective variants of BMV (B3DeltaMP) and CCMV (CC3DeltaMP). Neither NB-TMV-MP(+) nor NB-RCNMV-MP(+) was able to rescue the defective cell-to-cell and long-distance movement of B3DeltaMP. In contrast, NB-RCNMV-MP(+) complemented the cell-to-cell, but not the long-distance, movement of CC3DeltaMP. Taken together, these studies suggest that virus movement is a complex process and that, in some cases, the host species plays a major role in determining the long-distance movement function of a virus.  相似文献   

Responses of buffaloes to heavy working load under tropical conditions     

R.C. Upadhyay  M.V.N. Rao 《Livestock Production Science》1985,13(2):199-203

Four male buffaloes of 3 to $\text{3}\text{1}\text{2}$ years were subjected to heavy loads during hot dry conditions for 3 h continuously. Rectal temperature, respiration rate and heart rate increased significantly. The blood pH, P_O2, increased significantly and P_CO2 declined. During work, buffaloes were not able to eliminate heat produced and exhibited such distress symptoms as profuse salivation, frothing, lack of leg co-ordination, excitement and tongue protrusion. The muzzle secretion of buffaloes increased greatly. Even 3 h after discontinuation of work, the physiological reactions had not fully disappeared.  相似文献   

Influence of sources and doses of N and K on herbage,oil yield and nutrient uptake of patchouli [Pogostemon cablin (Blanco) Benth.] in semi-arid tropics     

Munnu Singh  R.S. Ganesha Rao 《Industrial Crops and Products》2009,29(1):229-234

Patchouli oil is one of the most important essential oils used in modern perfumery and cosmetic industries. There is hardly any preparation of oriental nature where patchouli oil is not used. It is used mainly because of fixative property as it gives tenacity to other perfumes. Field experiments were conducted at Bangalore, India, to study the influence of sources and doses of N and K on herbage, oil yield, nutrient uptake, nitrogen utilization efficiency and oil content of patchouli [Pogostemon cablin (Blanco) Benth.]. The results revealed that application of 200 kg N/ha and 41.5 kg K/ha produced significant higher patchouli herbage and oil yields compared with controls. Similarly, N and K uptake were also higher at 200 kg N and 41.5 kg K/(ha year) compared with controls. DCD-coated urea produced higher herbage, oil yield and N uptake and utilization efficiency compared with prilled urea. There was no effect of sources of K on the yield of patchouli. The oil content was not influenced by N, K doses or sources applied. N and K depletion were noticed in the soil.  相似文献   

海拨高度与肉鸡腹水征的相关性研究     

饶家荣  李权 《中国家禽》1999,(2)

选择云南省海拔高度76．4m～3200m不同的5个地、县自然发生的肉鸡腹水综合征（ASB）与同品种的健康鸡进行对比研究。结果证实，同品种肉鸡喂同种饲料并在相同的饲养管理条件下，无论在低海拔地区还是高海拔地区发病相同，肉鸡腹水综合征的发生与海拔高低无关。  相似文献   

运用QSAR模式对N—甲基氨基甲酸酯类农药急性毒性的定量预测     

刘兴泉  张子丰 《吉林农业大学学报》1998,20(3):50-53

运用多元回归对２２种Ｎ－甲基氨基酸甲酸酯类农药进行了结构－毒性关系的研究，选入的结构参数ｌｏｇＫｏｗ和分子连接性指数直接通过计算得到，毒性参数诜在量鼠经口ＬＤ６５０。结果表明，ｌｏｇＫｏｗ，ＸＶ和ＸＶ能很好地反映这类化合物的结构特性。  相似文献   

计算法测量电阻应变原理的试验分析     

饶秀勤  吴国桢 《浙江大学学报(农业与生命科学版)》1998,(3)

介绍了计算法测量电阻应变的原理及试验，着重分析了试验结果，验证了测量原理．  相似文献   

Interaction between Eimeria uzura infection and aflatoxicosis in Japanese quail (Coturnix coturnix japonica)     

J R Rao  N N Sharma  P K Iyer  A K Sharma 《Veterinary parasitology》1990,35(3):259-267

The clinical signs and gross lesions caused by Eimeria uzura (10(5) oocysts) in Japanese quail (Coturnix coturnix japonica) exhibited little or no influence in the face of intercurrent dietary aflatoxicosis (1 p.p.m. of aflatoxin B1 from Day 0 to 55). Similarly, no significant differences in the mucosal morphology of the intestine were evident histologically between the two groups of Japanese quail. The nervous signs of ataxia, leg weakness, incoordination of movement, torticollis and terminal opisthotonos were toxin-induced manifestations. In the aflatoxic quail, hypoplastic changes and selective depletion of lymphocytes were more prominent in the bursa of fabricius. Increased relative mean weights of liver, kidney, spleen, crop, proventriculus and gizzard were observed in birds due to aflatoxin sensitivity. The combination of E. uzura infection and aflatoxicosis in Japanese quail may cause significant weight loss, and increased oocyst production and reproductive potential.  相似文献   

Failure of excess leucine to impact live performance and carcass traits in male and female Cobb MV × 500 broilers during a 15- to 32-day grower period     

C.W. Maynard  G.J. Mullenix  C.J. Maynard  J.T. Lee  S.K. Rao  L.D. Butler  J.Z. Hiltz  S.K. Orlowski  M.T. Kidd 《The Journal of Applied Poultry Research》2022,31(2):100242

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