Growth and age composition of northern shrimp Pandalus eous estimated by multiple length frequency analysis     

Hiroshi Yamaguchi  Yoko Goto  Noboru Hoshino  Kazushi Miyashita 《Fisheries Science》2014,80(4):665-678

We examined growth of northern shrimp Pandalus eous in the Sea of Japan, off western Hokkaido, to improve estimations of catch-at-age for stock assessment. Multiple length frequency analysis based on length frequency data collected by a scientific research vessel was conducted to examine length-at-age in the shrimp population. Multi-normal distributions estimated using maximum likelihood indicated a good fit to length distributions. AIC values and regression analyses revealed annual growth variation and a decreasing trend in the length at several age classes in the shrimp population. We revised the method for estimating catch-at-age from the age-conversion table (ACT), which is a simple method for age determination, to age–length keys (ALK) calculated from the results of multiple length frequency analysis. Abundant year classes caught successively year after year could be more easily identified from the catch-at-age data computed using ALK than by using ACT. Our results suggested not only that the mean size of commercial landings fluctuated based on changes in age composition but also that a decrease in the length-at-age in the population influenced the consistent size decrease of commercial landings.  相似文献   

Development of a new selective medium for isolation of methicillin-resistant Staphylococcus aureus     

Saito E  Kakita Y  Shimoda H  Shimabukuro S  Saeki K  Shimizu A  Kawano J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1195-1197

A new selective medium containing cephem antibiotics was developed for isolation of methicillin-resistant Staphylococcus aureus (MRSA). MRSA colonies on a medium containing ceftazidime (CAZ) were most easily identifiable and a medium containing cefoperazone (CPZ) was superior in suppressing the growth of other bacteria. With the medium containing a couple of CAZ and CPZ, MRSA and methicillin-resistant coagulase-negative staphylococci (MRCNS) were detected from 2 and 1 of 15 chicken meat samples respectively. The MRSA and MRCNS recovery test showed that the medium was effective for MRSA isolation, suppressing the growth of other bacteria efficiently. These results suggested that the medium containing a couple of CAZ and CPZ was useful for MRSA detection from foods and animals.  相似文献   

Evaluation of miltefosine for the treatment of dogs naturally infected with L. infantum (=L. chagasi) in Brazil     

Andrade HM  Toledo VP  Pinheiro MB  Guimarães TM  Oliveira NC  Castro JA  Silva RN  Amorim AC  Brandão RM  Yoko M  Silva AS  Dumont K  Ribeiro ML  Bartchewsky W  Monte SJ 《Veterinary parasitology》2011,181(2-4):83-90

Dogs naturally infected with Leishmania Infantum (=L. chagasi) were treated with miltefosine using different therapeutic regimens. The animals were evaluated for clinical evolution, biochemical parameters, parasite load (by real-time PCR), cytokine levels and humoral response. After treatment and during the following 24 months, there was progressive clinical improvement and complete recovery in 50% (7/14) of the treated animals. There was a decrease in the smear positivity of the bone marrow after treatment, and there was also a gradual and constant decrease in positive cultures at the end of the follow-up period. However, the PCR detection of parasite DNA remained positive. In general, all animals presented a significant increase in parasite load 6 months after treatment. The IFN-γ levels in all the groups tended to increase during follow-up period, regardless of the miltefosine dose administered. The IL-4 and IL-10 levels of the animals tended to decrease during follow-up, except after 300 days when only IL-10 increased. The serum antibodies identified antigens that ranged from 116 kDa to less than 29 kDa in the Western blot assay. Furthermore, 300 days after treatment, qualitative and quantitative differences in the antigen profiles were observed. Antigens of 97 and 46 kDa were the most intensely recognized. Higher levels of antigen-specific Leishmania IgG were detected before and 300 days after treatment in all groups. Taking together, the improvement in the clinical symptoms was not followed by parasitological clearance, suggesting that treatment with miltefosine is not recommended, especially in endemic areas like Brazil, where children are the major victims and dogs are involved in the maintenance of the parasite cycle.  相似文献   

Effects of electroporation treatment using different concentrations of Cas9 protein with gRNA targeting Myostatin (MSTN) genes on the development and gene editing of porcine zygotes     

Quynh A. Le  Maki Hirata  Nhien T. Nguyen  Koki Takebayashi  Manita Wittayarat  Yoko Sato  Zhao Namula  Masahiro Nii  Fuminori Tanihara  Takeshige Otoi 《Animal Science Journal》2020,91(1)

This study was conducted to investigate the effect of seven concentrations of Cas9 protein (0, 25, 50, 100, 200, 500, and 1,000 ng/µl) on the development and gene editing of porcine embryos. This included the target editing and off‐target effect of embryos developed from zygotes that were edited via electroporation of the Cas9 protein with guide RNA targeting Myostatin genes. We found that the development to blastocysts of electroporated zygotes was not affected by the concentration of Cas9 protein. Although the editing rate, which was defined as the ratio of edited blastocysts to total examined blastocysts, did not differ with Cas9 protein concentration, the editing efficiency, which was defined as the frequency of indel mutations in each edited blastocyst, was significantly decreased in the edited blastocysts from zygotes electroporated with 25 ng/µl of Cas9 protein compared with that of blastocysts from zygotes electroporated with higher Cas9 protein concentrations. Moreover the frequency of indel events at the two possible off‐target sites was not significantly different with different concentrations of Cas9 protein. These results indicate that the concentration of Cas9 protein affects gene editing efficiency in embryos but not the embryonic development, gene editing rate, and non‐specific cleavage of off‐target sites.  相似文献   

Experimental model for removal of snake venom via hemoperfusion in rats     

Maximilian Estevan Oliveira  Jssica Campanholi  Roberta Lima Cavalcante  Felipe Silveira Moreno  Edson Hideaki Yoshida  Murilo Melo Juste Dini  lvio Franco de Camargo Aranha  Jos Carlos Cogo  Lourdes Dias  Stephen Hyslop  Denise Grotto  Valquíria Miwa Hanai‐Yoshida  Yoko Oshima‐Franco 《Journal of Veterinary Emergency and Critical Care》2020,30(3):286-294

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Temporal changes in clinical and radiographic variables in dogs with preclinical myxomatous mitral valve disease: The EPIC study     

Adrian Boswood  Sonya G. Gordon  Jens Häggström  Martin Vanselow  Gerhard Wess  Rebecca L. Stepien  Mark A. Oyama  Bruce W. Keene  John Bonagura  Kristin A. MacDonald  Mark Patteson  Sarah Smith  Philip R. Fox  Karen Sanderson  Richard Woolley  Viktor Szatmári  Pierre Menaut  Whitney M. Church  M. Lynne O'Sullivan  Jean-Philippe Jaudon  Jan-Gerd Kresken  John Rush  Kirstie A. Barrett  Steven L. Rosenthal  Ashley B. Saunders  Ingrid Ljungvall  Michael Deinert  Eric Bomassi  Amara H. Estrada  Maria J. Fernandez Del Palacio  N. Sydney Moise  Jonathan A. Abbott  Yoko Fujii  Alan Spier  Michael W. Luethy  Roberto A. Santilli  Masami Uechi  Anna Tidholm  Christoph Schummer  Philip Watson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(3):1108-1118

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Aluminum inhibits growth and stability of cortical microtubules in wheat (Triticum aestivum) roots     

Masao Sasaki  Yoko Yamamoto  Hideaki Matsumoto 《Soil Science and Plant Nutrition》2013,59(2):469-472

Aluminum (Al) occurs abundantly in soil and solubilized aluminum ions in acid soil inhibit plant growth, in particular, root growth. Although several toxic effects of Al on plant growth have been reported, the mechanism of Al toxicity remains to be clarified.  相似文献   

Effect of presowing seed treatments on germination and early seedling growth of wheat varieties under saline conditions     

Maqsood Ahmed Kamboh  Yoko Oki  Adachi Tadashi 《Soil Science and Plant Nutrition》2013,59(1):249-255

The effect of presowing wheat seed treatments was investigated for the salt-sensitive variety Blue Silver and relatively salt-tolerant variety PARI-73 at the germination and early seedling stages. Seeds were treated with distilled water (DW) or 10/50 mm KCl, KNO₃ , CaCl₂, Ca(NO₃)₂ and then germinated in DW or 200 mm NaCl. Treatment with calcium (Ca) or potassium (K) did not lead to a significantly higher rate for final germination than the DW treatment. Ca salt treatments significantly improved shoot growth during the early seedling establishment stage in both varieties, especially in the salt-sensitive variety Blue Silver. There were significant differences in the Ca content of seeds after various presowing treatments. There were also significant differences between both varieties in the ion contents after seed treatment. However, these differences appeared to be related to the improvement of shoot growth during the early seedling establishment stage and not to the effectiveness of pres owing seed treatments in increasing germination.  相似文献   

Effect of boiled barley-rice-feeding in hypercholesterolemic and normolipemic subjects     

Sachie Ikegami  Masako Tomita  Setsuko Honda  Momoko Yamaguchi  Reiko Mizukawa  Yoko Suzuki  Keiko Ishii  Saeko Ohsawa  Nanako Kiyooka  Mitsuru Higuchi  Shuhei Kobayashi 《Plant foods for human nutrition (Dordrecht, Netherlands)》1996,49(4):317-328

Barley contains approximately 10% dietary fiber and is easily cooked with rice, the dominant cereal in Japan, to increase the intake of dietary fiber. This research involved three experiments to examine the influence of barley on blood lipids in human subjects. All subjects received a boiled barley-rice (50/50 w/w mix) supplement two times per day in place of rice for 2 or 4 weeks. In the normolipemic subjects, serum lipids were unaffected by the ingestion of barley for 4 weeks. In twenty hypercholesterolemic men aged 41 ± 5 years, the ingestion of barley was associated with a significant fall in serum total cholesterol, LDL-cholesterol, phospholipids and LDL and VLDL-lipoproteins. In seven mildly hypercholesterolemic women aged 56±7 years, a significant improvement of serum lipid profiles was observed. The present study suggests the possibility that the ingestion of barley-rice could lower serum lipids in hypercholesterolemic subjects.  相似文献   

Clonal variation of carbon content in wood of Larix kaempferi (Japanese larch)   总被引：1，自引：0，他引：1  

Eitaro Fukatsu  Yoko Fukuda  Makoto Takahashi  Ryogo Nakada 《Journal of Wood Science》2008,54(3):247-251

Variations in carbon content in wood among 102 clones, selected from almost the entire natural distribution area, were investigated in Larix kaempferi. The average carbon content was 50.50%, 50.94%, and 50.80% in sapwood, heartwood, and whole wood, respectively. The difference in carbon content between clones was significant. The clonal repeatabilities were 0.46, 0.38, and 0.44 in heartwood, sapwood and whole wood, respectively. The coefficients of variation in the clonal mean carbon content were only 0.43%, 0.42%, and 0.41% in heartwood, sapwood, and whole wood, respectively. This small genetic variation and resulting small relative genetic gain of carbon content indicate that the genetic improvement of carbon content by selection has a small effect on the genetic improvement of carbon sequestration capacity by selection in L. kaempferi.  相似文献