Detachable Coils for Occlusion of Patent Ductus Arteriosus in 2 Dogs     

Ryou Tanaka  DVM  PhD    Katsuichiro Hoshi  DVM    Yukiko Nagashima  DVM    Yoko Fujii  DVM    Yoshihisa Yamane  DVM  PhD 《Veterinary surgery : VS》2001,30(6):580-584

OBJECTIVE: To describe the use of a detachable coil for transcatheter closure (TCC) of patent ductus arteriosus (PDA) in 2 dogs. STUDY DESIGN: Clinical study. ANIMALS: Two female Pembroke Welsh Corgi dogs with PDA. METHODS: Using fluoroscopic guidance, an 8-mm-diameter coil stent with 5 loops (detachable coils for PDA closure) was inserted via catheterization of the femoral artery. The catheter was passed through the PDA into the pulmonary artery. The coil was withdrawn so that 1.5 loops remained on the pulmonary side of the orifice of the ductus. The rest of the loops were pushed out from the catheter into the ductus. After confirming the correct placement of the coil and the effectiveness of the occlusion, the delivery wire was detached from the coil. RESULTS: Insertion of the coil was easily performed, even without previous experience. Immediate and marked decrease of the cardiac murmur was auscultated. Only slight residual flow was detected by angiography conducted 3 months' postoperatively. The dogs experienced quick and uneventful recovery after coil placement and required minimal postoperative care. Follow-up evaluation of the dogs showed no functional clinical signs of PDA, and no cardiac abnormalities were detected on electrocardiographic, phonocardiographic, and echocardiographic examination. In dog 1, the residual flow had disappeared on the color-flow Doppler echocardiographic examination at 18 months' postoperatively. CONCLUSION: TCC using a detachable coil was easy, safe, and effective in 2 dogs with PDA. The minimal residual shunting observed only by echocardiography seemed hemodynamically insignificant. CLINICAL RELEVANCE: This method can be used as an alternative to traditional surgical methods.  相似文献   

Geographical distribution and seasonality of the prevalence of Leucocytozoon lovati in Japanese rock ptarmigans (Lagopus mutus japonicus) found in the alpine regions of Japan     

Murata K  Tamada A  Ichikawa Y  Hagihara M  Sato Y  Nakamura H  Nakamura M  Sakanakura T  Asakawa M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(2):171-176

In this study, we investigated the geographical distribution and seasonality of Leucocytozoon lovati infection in the Japanese rock ptarmigan (Lagopus mutus japonicus); this bird is one of the special natural monuments of Japan that inhabits the Japanese alpine regions. We examined blood samples from birds captured in the Kubiki, Hida, and Akaishi mountain ranges for three years from 2002 to 2005. Seventy-three blood samples from 42 males, 19 females, and 12 birds of unknown sex were used for this study. The rate of infection with L. lovati was 78.1% in the 73 birds examined. We demonstrated that the L. lovati infection was distributed across wide ranges of ptarmigan populations from the northern to the southern alpine zones. There was no sex bias in the prevalence ratio. The prevalence of L. lovati and the level of parasitization of the blood cells tended to increase from spring through summer; in contrast, a decrease was observed from summer through autumn. Although L. lovati infection was observed in a number of local populations inhabiting three mountainous regions, no infected birds were found in Mt. Johnen-dake and Mt. Maejohnen-dake. It is necessary to continue surveying the relationship between the population dynamics of the ptarmigan and the density of the arthropod vector from the perspective of in situ conservation of this endangered species.  相似文献   

Three-dimensional culture of keratinocytes and the formation of basement membrane for canine footpad substitute   总被引：1，自引：0，他引：1  

Yamazoe K  Miyamoto S  Hikosaka Y  Kitagawa K  Watanabe K  Sakai H  Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):611-617

A pad equivalent for a dog was prepared as a substitute for the loss of footpad. In addition to the time course of formation on epidermal morphogenesis, we investigated expressions of alpha(6) integrin subunit as adhesive molecule, and laminin and type IV and VII collagens as extracellular matrices of basement membrane components. Epithelium of the pad equivalent was thick enough to be easily confirmed at 5 days at the air-liquid interface, but many creases appeared on it at 7 days, and it shrank at 10 and 14 days. Keratinocytes were increased in 4 to 5 cell layers at 1 day at the air-liquid interface, differentiating into basal cell layer. Granular and corneal cell layers were confirmed until 5 days, and maintained their shape at least until 14 days. Alpha 6 integrin was expressed at almost the same fluorescent intensity as native pad tissue at 1 day at the dermal-epidermal junction. Laminin and type IV collagen were intermittently expressed at 5 and 10 days, respectively, at the dermal-epidermal junction, and at 14 days the fluorescence showed almost the same intensity as native pad tissue. The expression of type VII collagen was discontinuous at 2 days at the dermal-epidermal junction, but remained as it was at 14 days. The present findings suggested that although the formation of anchoring fibrils in basement membrane was incomplete, the pad equivalent in the dog was reconstructed similar to a native pad by epidermal morphogenesis.  相似文献   

Gene expression in individual bovine somatic cell cloned embryos at the 8-cell and blastocyst stages of preimplantation development   总被引：2，自引：0，他引：2  

Amarnath D  Li X  Kato Y  Tsunoda Y 《The Journal of reproduction and development》2007,53(6):1247-1263

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Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus     

Naoyuki AIHARA  Noriyuki HORIUCHI  Nanase HIKICHI  Mariko OCHIAI  Yuko HOSODA  Yoko ISHIKAWA  Yoko SHIMAZAKI  Koji OISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):913-918

Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine strain-administered groups (vaccine groups), and the level of severity was the same as that in the wild-type strain-administered group (wild-type group). However, these chickens retained humoral antibody responses to NDV and were revealed to possess a higher number of bursal follicles than those of the wild-type group. These results indicated that macroscopic evaluation dose not accurately reflect the immunoreactivity and degree of bursal damage in IBDV-administered chickens. We also found non-immunosuppressed chickens in the wild-type group. These non-immunosuppressed chickens retained a significantly higher number of normal follicles and total follicles according to our statistical analysis. Furthermore, a high correlation coefficient between the NDV-HI titer and the number of normal follicles was found in the wild-type group. These results implied that the retained number of normal follicles is important for the immunoreactivity of chickens infected with IBDV.  相似文献   

Evaluation of the transmission risk of foot-and-mouth disease in Japan     

Yoko HAYAMA  Takehisa YAMAMOTO  Sota KOBAYASHI  Norihiko MUROGA  Toshiyuki TSUTSUI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1167-1170

The transmission risk of foot-and-mouth disease (FMD) in Japan was evaluated using a mathematical FMD transmission model. The distance-based transmission rate between farms, which was parameterized using the FMD epidemic data in 2010 in Japan, was used to calculate the local-level reproduction numbers—expected numbers of secondary infections caused by one infected farm—for all cattle and pig farms in the country, which were then visualized as a risk map. The risk map demonstrated the spatial heterogeneity of transmission risk in the country and identified risk areas with higher possibility of disease spread. This result suggests that, particularly in high-risk areas, it is important to prepare for the smooth and efficient implementation of control measures against FMD outbreaks.  相似文献   

Feasibility of radial and circumferential strain analysis using 2D speckle tracking echocardiography in cats     

Hiroshi TAKANO  Tomomi ISOGAI  Takuma AOKI  Yoshito WAKAO  Yoko FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):193-201

The purpose of the present study is to investigate the feasibility of strain analysis using speckle tracking echocardiography (STE) in cats and to evaluate STE variables in cats with hypertrophic cardiomyopathy (HCM). Sixteen clinically healthy cats and 17 cats with HCM were used. Radial and circumferential strain and strain rate variables in healthy cats were measured using STE to assess the feasibility. Comparisons of global strain and strain variables between healthy cats and cats with HCM were performed. Segmental assessments of left ventricle (LV) wall for strain and strain rate variables in cats with HCM were also performed. As a result, technically adequate images were obtained in 97.6% of the segments for STE analysis. Sedation using buprenorphine and acepromazine did not affect any global strain nor strain rate variable. In LV segments of cats with HCM, reduced segmental radial strain and strain rate variables had significantly related with segmental LV hypertrophy. It is concluded that STE analysis using short axis images of LV appeared to be clinically feasible in cats, having the possibility to be useful for detecting myocardial dysfunctions in cats with diseased heart.  相似文献   

Substrate specificity of the nateglinide/H(+) cotransport system for phenolic acids   总被引：2，自引：0，他引：2  

Saito Y  Itagaki S  Otsuka Y  Kobayashi Y  Okumura H  Kobayashi M  Hirano T  Iseki K 《Journal of agricultural and food chemistry》2005,53(15):6100-6104

In clinical, patients usually take many kinds of drugs at the same time. Thus, drug-drug interactions involving transporters can often directly affect the therapeutic safety and efficacy of many important drugs. However, there have been few studies on food-drug interactions involving transporters. Dietary polyphenols have been widely assumed to be beneficial for human health. Polyphenols are found ubiquitously, and they are commercially prepared and used as functional foods. We have reported that ferulic acid, which is one of the most well-known polyphenols and is used as a functional food, affected the transport of nateglinide, an antidiabetic drug, by Caco-2 cells. In this study, we investigated the effects of other polyphenols on the nateglinide/H(+) transport system. We report here that caffeic acid and p-coumaric acid have a different inhibitory manner on the uptake of nateglinide. The results of this study are useful to identify the substrate specificity of the nateglinide/H(+) cotransporter.  相似文献   

Determination of neonicotinoid pesticide residues in vegetables and fruits with solid phase extraction and liquid chromatography mass spectrometry   总被引：9，自引：0，他引：9  

Obana H  Okihashi M  Akutsu K  Kitagawa Y  Hori S 《Journal of agricultural and food chemistry》2003,51(9):2501-2505

A rapid and simple extraction method for the simultaneous analysis of five neonicotinoid insecticides has been developed. Twelve different fruit and vegetable matrixes were extracted with methanol and cleaned up using a graphitized carbon solid phase extraction cartridge loading with a 20% methanol solution. The concentrated eluate after methanol elution was then analyzed for pesticide residues by liquid chromatography/mass spectrometry in the APCI positive mode. The five pesticides including nitenpyram, thiamethoxam, imidacloprid, acetamiprid, and thiacloprid were recovered at 70-95% at spike levels of 0.1 and 1 mg/kg in bell pepper, cucumber, eggplant, grape, grapefruit, Japanese radish, peach, pear, potato, rice, and tomato. Relative standard deviations were less than 10% for all of the recovery tests. The proposed method is fast, easy to perform, and could be utilized for regular monitoring of pesticide residues.  相似文献   

The impact of aluminium on the distribution of cell wall glycoproteins of pea root tip and their Al-binding capacity     

Saule Kenjebaeva  Yoko Yamamoto 《Soil Science and Plant Nutrition》2013,59(3):629-636

Pea (Pisum sativum L.) roots were exposed to various concentrations of Al and cell wall glycoproteins containing salt-extractable proteins, SDS-extractable proteins, covalently bound proteins (CBPs), and extensins were separately extracted. The changes in their levels and the amount of bound Al to glycoproteins upon Al stress were investigated. The content of CBPs, but not of others, increased significantly with the increase of Al levels. Extensins and the salt-extractable proteins, but not the others, exhibited a marked Al-binding capacity in vivo. The amount of Al bound to extensions in vivo and in vitro was highest among the glycoproteins. The high Al-binding capacity of extensins might be caused by the high content of the hydroxy groups in hydroxyproline of extensins and Al-binding capacity to glycoproteins in the root tips may be involved in the Al-binding response in the apoplast.  相似文献