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991.
Downy mildew, caused by Pseudoperonospora cubensis (Berk. & Curt.) Rostov, is one of the most economically important foliar diseases in cucumber (Cucumis sativus L.). Cucumber line CS-PMR1, derived from self-pollination of USDA Plant Introduction 197088, has a high level of resistance to downy mildew and is considered to be promising breeding material. In this study, we performed quantitative trait locus (QTL) analysis for downy mildew resistance using 111 recombinant inbred lines (RILs) derived from a cross between CS-PMR1 and the old Japanese cultivar Santou, which exhibits moderate resistance. The resistance of the RILs and their parents was evaluated by diverse methods using different plant organs (cotyledons, true leaves), stages (seedlings and adult plants), and evaluation criteria (lesion expansion and extent of sporulation). The high resistance of CS-PMR1 was associated with many QTLs with relatively small effects, whereas the moderate resistance of Santou was associated with one major QTL and possibly two others with relatively small effects. In all assays, the major QTL at which the Santou allele was associated with increased resistance had the largest effect. This QTL allele from Santou and several of the most effective QTL alleles identified in CS-PMR1 should be highest priority for selection to efficiently breed new cultivars that carry adequate levels of downy mildew resistance. 相似文献
992.
Yuta Inagaki Tetsuya Takatsu Masafumi Kimura Yota Kano Toyomi Takahashi Yoshihiko Kamei Naoto Kobayashi Tatsuaki Maeda 《Fisheries Science》2014,80(4):725-734
To identify the factors that influence the growth rate of flathead flounder Hippoglossoides dubius in Funka Bay, Japan, we studied temporal changes in the growth of this species and its prey from 1989 to 2012. The lowest growth rate of H. dubius was recorded in the 1995 year-class, which had experienced severe hypoxia between 1995 and 1997. However, the highest growth rate was recorded in the 2007 year-class, which had experienced hypoxia between 2009 and 2011. In 2000 and 2001, small-sized flounder (<200 mm TL) from the 1995 year-class were feeding on mysidaceans, whereas those from other year-classes fed on small Ophiura spp. (<9 mm in disc diameter) in 2009, 2011, and 2012. Small Ophiura spp. were more abundant in 2007 and 2011 than in 2002 and 2003. Successful recruitment of Ophiura spp. took place between 2007 and 2011 and cohorts from these years could tolerate hypoxia. This suggests that food availability improved under hypoxic conditions, which led to improved growth in small flounders. Large flounders (≥200 mm TL) from all year-classes fed on prickleback fishes (Stichaeidae) and walleye pollock Gadus chalcogrammus juveniles. The growth of large flounders was enhanced by an increase in the abundance of Stichaeidae fishes and G. chalcogrammus juveniles. 相似文献
993.
Yosuke Tanaka Hiroshi Minami Yuka Ishihi Kazunori Kumon Kentaro Higuchi Takeshi Eba Akefumi Nishi Hideki Nikaido Satoshi Shiozawa 《Fisheries Science》2014,80(6):1205-1214
Fast growth plays an important role in survival processes during the early life stages of both field-captured and hatchery-reared Pacific bluefin tuna Thunnus orientalis. Marked growth variations in hatchery-reared tuna larvae are frequently observed even for the same age and within the same rearing tank after the onset of the piscivory. We hypothesized that these small growth variations in the growth of tuna larvae at the onset of piscivory lead subsequently to large growth variations and tested the hypothesis using three size groups (large, intermediate and small) of hatchery-reared fish by nitrogen stable isotope and otolith analyses. Stable isotope analysis revealed that the large group rapidly utilized prey fish larvae, but the smaller groups depended more on rotifers as the main prey item relative to the large group. The otolith radius from the core to the increment corresponding to the first feeding on yolk-sac larvae was compared among the three size groups. The results revealed that the large group had larger otolith radii than the small and intermediate groups. Our findings suggest that small growth variations apparent during the early larval stage of tuna could induce further large growth variations in the late-larval and juvenile stages through differences in the initial ability to utilize piscivory. 相似文献
994.
Mortality processes of hatchery‐reared Pacific bluefin tuna Thunnus orientalis (Temminck et Schlegel) larvae in relation to their piscivory
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Yosuke Tanaka Kazunori Kumon Yuka Ishihi Takeshi Eba Akefumi Nishi Hideki Nikaido Satoshi Shiozawa 《Aquaculture Research》2018,49(1):11-18
In mass culture of Pacific bluefin tuna Thunnus orientalis, yolk‐sac larvae of other species are fed as a major prey item to tuna larvae from 7 to 8 mm in total length. Marked growth variations in tuna larvae are frequently observed after feeding of yolk‐sac larvae, and this variation in the growth of tuna larvae is subsequently a factor leading to the prevalence of cannibalistic attacks. To elucidate details of the mortality process of hatchery‐reared tuna larvae after the initiation of yolk‐sac larvae feeding, we compared the nutritional and growth histories of the surviving (live) tuna larvae to those of the dead fish, found dead on the bottom of the tank, as direct evidence of their mortality processes. Cause of mortality of tuna larvae 3 and 5 days after the initiation of feeding of yolk‐sac larvae was assessed from nitrogen stable isotope and otolith microstructure analyses. Stable isotope analysis revealed that the live fish rapidly utilized prey fish larvae, but the dead fish had depended more on rotifers relative to the live fish 3 and 5 days after the initiation of feeding of yolk‐sac larvae. The growth histories based on otolith increments were compared between the live and dead tuna larvae and indicated that the live fish showed significantly faster growth histories than dead fish. Our results suggest that fast‐growing larvae at the onset of piscivory could survive in the mass culture tank of Pacific bluefin tuna and were characterized by growth‐selective mortality. 相似文献
995.
996.
Kentaro Higuchi Yosuke Tanaka Takeshi Eba Akefumi Nishi Kazunori Kumon Hideki Nikaido Satoshi Shiozawa 《Fisheries Science》2013,79(1):111-117
High mortality of hatchery-reared Pacific bluefin tuna (PBT) juveniles is frequently observed in sea cages after transfer from indoor tanks, and is likely due to collisions of the fish with the net wall of the sea cages. Because collisions frequently cause bone injuries due to physical impact, such injuries can be considered an index of obvious collisions. In the present study, bone injuries of dead juveniles were investigated in order to examine the prevalence of net collision as a cause of death among hatchery-reared PBT juveniles in sea cages. Juvenile PBT were reared in sea cages for 90 days after transfer, and dead fish were collected and examined by the clearing and staining method or by dissection to detect injury to the vertebral column and parasphenoid. The prevalence of injuries to the vertebral column and parasphenoid until day 30 after transfer was low (0.0–12.0 %) when the fish were 5.5–15.2 cm in total length (TL). However, it drastically increased to 17.8–78.0 % for fish 21.0–39.2 cm TL from day 31 onward after transfer. These results suggest that the mortalities of juvenile PBT larger than 20 cm TL in cages were induced by net collisions. 相似文献
997.
Mohammad Bannayan Kazuhiko Kobayashi Han-Yong Kim Mark Lieffering Masumi Okada Shu Miura 《Field Crops Research》2008,107(3):276
The publisher regrets that the following error has occurred in the above article: page 239, Table 1, and page 247, Table 5 should be replaced with below Tables in the original printing of the above-mentioned paper. 相似文献
998.
Itagaki S Ochiai A Kobayashi M Sugawara M Hirano T Iseki K 《Journal of agricultural and food chemistry》2008,56(16):6923-6927
In clinical trials, patients usually take many kinds of drugs at the same time. Thus, drug-drug interactions can often directly affect the therapeutic safety and efficacy of many drugs. Oral delivery is the most desirable means of drug administration. Changes in the activity of drug transporters may substantially influence the absorption of administered drugs from the intestine. However, there have been a few studies on food-drug interactions involving transporters. It is important to be aware of the potential of food-drug interactions and to act in order to prevent undesirable and harmful clinical consequences. Coenzyme Q10 (CoQ10) is very widely consumed by humans as a food supplement because of its recognition by the public as an important nutrient in supporting human health. Since intestinal efflux transporter P-glycoprotein (P-gp) is one of the major factors in drug-drug interactions, we focused on this transporter. We report here for the first time that CoQ10, which is widely used as a food supplement, affects the transport activity of P-gp. 相似文献
999.
Mitsuhiro Sugiyama Yosuke Yoshioka Yoshiteru Sakata 《Journal of General Plant Pathology》2009,75(5):381-387
Melon yellow spot virus (MYSV), a member of the genus Tospovirus, is a devastating thrips-transmitted virus of cucurbits in Japan. Recently, we reported that cucumber accessions originating
from South Asia, in particular Southeast Asia, had moderate resistance to MYSV. Here, we investigated the effect of three
temperatures (20°C, 25°C, and 30°C) on symptom expression and viral spread of MYSV in plants of resistant cucumber accessions.
No systemic infection developed in resistant cucumber plants after inoculation with melon isolate MYSV-S at low temperature
(20°C); viral spread of MYSV-S and cucumber isolate MYSV-FuCu05P in inoculated cotyledons was suppressed. In contrast, higher
incubation temperatures (25°C and 30°C) facilitated viral spread in inoculated cotyledons and systemic infection of MYSV-S.
These data suggest that the resistance to MYSV of resistant cucumber accessions is temperature dependent. 相似文献
1000.
Marcelo Andrade Yosuke Abe Kenji S. Nakahara Ichiro Uyeda 《Journal of General Plant Pathology》2009,75(3):241-249
The same mutant allele of eukaryotic initiation factor 4E (eIF4E) that confers resistance to Pea seed-borne mosaic virus (sbm-1) and the white lupine strain of Bean yellow mosaic virus (wlv) also confers resistance to Clover yellow vein virus (ClYVV) in pea. The eIF4E genes from several pea lines were isolated and sequenced. Analysis of the eIF4E amino acid sequences from several resistant
lines revealed that some lines, including PI 378159, have the same sequence as reported for sbm-1 and wlv. When eIF4E from a susceptible pea line was expressed from a ClYVV vector after mechanical inoculation of resistant PI 378159, the virus
caused systemic infection, similar to its effects in susceptible line PI 250438. The resistance to ClYVV in line PI 378159
was characterized through a cross with PI 193835, which reportedly carries cyv-2. Mechanical inoculation of the F1 progeny with ClYVV resulted in no infection, indicating that the resistance gene in PI
378159 is identical to cyv-2 in PI 193835. Furthermore, particle bombardment of pea line PI 193835 with infectious cDNA of ClYVV (pClYVV/C3-S65T) resulted
in the same resistance mode as that described for PI 378159. These results demonstrate that the resistance to ClYVV conferred
by cyv-2 is mediated by eIF4E and that cyv-2 is identical to sbm-1 and wlv. 相似文献