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101.
102.
We determined a suitable amount of fertilizer for konara oak(Quercus serrata) and Japanese zelkova(Zelkova serrata) planted in a harvested pitch pine(Pinus rigida) plantation. Two-year-old bare-root seedlings of konara oak and one-year-old containerized seedlings of Japanese zelkova were planted in April 2011. Three plots were established for each tree species to evaluate each of three fertilization applications. Solid compound fertilizer(N:P:K = 3:4:1) was applied yearly in three amounts(control: no fertilization, F1: 180 kg ha-1, and F2:360 kg ha-1), every May from 2011 to 2013. We measured the root collar diameter and height, and analyzed the compartmental N and P concentrations. Compartmental N concentrations of konara oak and Japanese zelkova were not consistent based on amount of fertilization. However,the compartmental P concentrations of konara oak and Japanese zelkova were significantly different in the order of F2, F1, and control. Although the differences in growth of konara oak appeared after 3 years of fertilization,Japanese zelkova showed differences after only 2 years of fertilization owing to differences in seedling type. Growth of konara oak was affected by fertilization at F1 and F2 in2013. However, growth of Japanese zelkova was affected only at F2. Nutrient demand of Japanese zelkova appeard to be higher than that of konara oak, at least during the early growing period. Results from this study could be practically used in harvested pitch pine plantations to determine appropriate fertilization regimes.  相似文献   
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104.
Two new briarane-type diterpenoids, briarenolides K (1) and L (2), were isolated from an octocoral identified as Briareum sp. The structures of new briaranes 1 and 2 were elucidated by spectroscopic methods. In the in vitro anti-inflammatory effects test, briaranes 1 and 2 were found to significantly inhibit the accumulation of the pro-inflammatory iNOS protein of the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells.  相似文献   
105.
Chen  Yu-Chun  Lin  Chun-Lan  Li  Chih-Ting  Hwang  Deng-Fwu 《Fisheries Science》2015,81(4):787-794
Fisheries Science - The aim of this study was to evaluate the antibacterial properties of oyster, hard clam, and sea urchin shell powders as a result of calcination. After subjection to calcination...  相似文献   
106.
Plasmodiophora brassicae causes clubroot of crucifers. A quantitative PCR (qPCR)‐based protocol was developed to measure P. brassicae DNA in the roots of susceptible, intermediately susceptible, intermediately resistant and resistant Brassica hosts, and the non‐host wheat, at 5, 10, 15, 20 and 42 days post‐inoculation (dpi). The final reaction of each plant genotype was recorded as an index of disease at 42 dpi. Plasmodiophora brassicae DNA showed an increase in susceptible and moderately resistant hosts from 5 to 42 dpi, in contrast to a decrease in a highly resistant host and the non‐host wheat over the same period. Index of disease was significantly positively correlated with the amount of P. brassicae DNA in the roots at 5, 15, 20 and 42 dpi in one experiment, and at 10, 15, 20 and 42 dpi in a repeated experiment. Significant positive correlations also existed between the amounts of P. brassicae DNA in the roots at 42 dpi and those at 5, 10, 15 and 20 dpi in one experiment, and those at 10, 15 and 20 dpi in a repeated experiment. The results generated by the qPCR assay were validated by microscopic examination of roots inoculated with P. brassicae. The qPCR‐based protocol developed in this study allows for the accurate quantification of P. brassicae DNA in host root tissues as early as 5 dpi, and may serve as a useful tool to evaluate pathogen proliferation and development in the roots.  相似文献   
107.
A 3-year-old, neutered male Persian cat with chronic ulcerative facial dermatitis was diagnosed with feline idiopathic facial dermatitis based on signalment, clinical history and diagnostic test results, including dermatohistopathological evaluation. Initial treatment started with 4 weeks of oral antifungal/antibiotic medication for severe secondary infectious dermatitis of Malassezia and bacteria. As the lesions gradually improved, the oral medication was withdrawn, leaving only 0.1% topical FK506 (tacrolimus) ointment for the remaining lesions. Topical treatment was administered just in case any new lesions developed. The patient has been managed effectively with topical tacrolimus and no side-effects were observed during treatment. Feline idiopathic facial dermatitis is known as a difficult dermatosis to manage successfully, but our experience suggests that it may respond to topical tacrolimus.  相似文献   
108.
We investigated the effects of hepatic and renal impairment on the pharmacokinetics of enrofloxacin in Sprague-Dawley rats. Experimental hepatic and renal failure were induced by carbon tetrachloride (CCL4) and 5/6 nephrectomy, respectively. After intravenous dosing of enrofloxacin (10 mg/kg), plasma concentrations of enrofloxacin were measured using liquid chromatograph/mass spectrometry. There was no significant effect of hepatic impairment on enrofloxacin pharmacokinetics. However, renal impairment markedly prolonged elimination half life (t1/2λz) of enrofloxacin (P < 0.05), comparing with respective control. Total body clearance (Clb) and volume of distribution at steady state (Vss) were significantly decreased (P < 0.05) by renal impairment. In conclusion, these results suggested that renal impairment could affect the pharmacokinetics of enrofloxacin.  相似文献   
109.
Discovery of Helicobacter (H.) pylori has led to a fundamental change in our understanding of gastric diseases in humans. Previous studies have found various Helicobacter spp. in dogs and cats, and pets have been questioned as a zoonotic carrier. The present study surveyed the Helicobacter infections and investigated the presence of H. felis and H. pylori infections in domestic and feral cats in Korea. Sixty-four domestic cats and 101 feral cats were selected from an animal shelter. Saliva and feces were evaluated by Helicobacter genus-specific polymerase chain reaction (PCR). Genus-specific PCR positive samples were further evaluated for H. felis and H. pylori using specific primer pairs. Thirty-six of 64 (56.3%) samples from domestic cats and 92 of 101 (91.1%) samples from feral cats were PCR positive; the positive rate of feces samples was higher than that of saliva samples in both groups. H. felis and H. pylori species-specific PCR was uniformly negative. The prevalence of Helicobacter spp. in feral cats was approximately two-fold higher than that of domestic cats. The fecal-oral route may be more a common transmission route not only between cats but also in humans.  相似文献   
110.
Fatty acid composition and C18:2:C18:3 of soybean seed and sprout is important for soy-industries to manufacture quality products. Six soybean varieties were analyzed by gas chromatography to determine fatty acid composition and linoleic to linolenic acid ratio (C18:2:C18:3) in seed, sprout, and their structural parts. In the case of whole seed and its structural parts, significant variation in fatty acid composition and C18:2:C18:3 were observed between varieties except palmitic acid (C16:0), while all the parameters were significantly different for seed parts. Significant interactions of variety with seed parts were observed for oleic acid (C18:1), C18:2 and C18:2:C18:3. The highest saturated fatty acid, C18:1 and polyunsaturated fatty acid were recorded in seed coat, cotyledon, and seed axis, respectively. The lowest C18:2:C18:3 was found in seed axis. In the case of sprout study, variety had significant effect on all the parameters observed for whole sprout, cotyledon, root, and except C18:3 for hypocotyl. Culture days had significant effect on C16:0, C18:2 and C18:3 in whole sprouts, while only on C18:3 and C18:2:C18:3 in cotyledon. For hypocotyl, culture days had significant effect on C18:1, C18:2 and C18:2:C18:3, however, C16:0, C18:1, C18:3, and C18:2:C18:3 were significantly different in root. In sprout, days and variety interacted significantly for C16:0, C18:3 and C18:2:C18:3, and C18:1, C18:2, C18:3, and C18:2:C18:3, respectively for whole, cotyledon, and root. The significantly lowest C18:2:C18:3 (1.1) was observed in hypocotyl and root of Hwangkeumkong in 5 days. Knowledge of fatty acid composition and C18:2:C18:3 of seed, sprout, and their parts could be applicable for oil and other soy-food industries to make quality products.  相似文献   
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