鲜王浆中超氧化物歧化酶的初步研究   总被引：1，自引：0，他引：1  

袁耀东  何薇莉 《中国蜂业》1993,(5):3-5

本工作以一种非酶体系核黄素、四甲基乙二胺光照产生 Oi 氧化羟胺形成亚硝酸盐为原理测定 SOD 活性的新方法对新鲜蜂王浆中超氧化物歧化酶(SOD)的活性进行测定,结果表明,新鲜蜂王浆中存在 SOD,其活性含量大约为0.403ng/g。  相似文献   

小麦矮秆育种中性状间关系的多元分析   总被引：12，自引：0，他引：12  

何中虎  张树榛 《华北农学报》1992,7(1):1-7

以7个株高梯度系列的35个系统及5个亲本品种为材料,采用典范分析和逐步回归分析研究了株高及其构成因子与产量因子、产量生理性状间的关系。结果表明:①株高因子通过产量生理性状影响产量因子,一定的生物学产量是获得高产的基础。②矮秆品种的高化对提高产量、千粒重和收获指数有利,对蛋白质含量的影响不明显;高秆品种矮化能显著提高收获指数,对籽粒的蛋白质含量影响不大。③在主要性状符合育种目标的前提下,选择倒二节间较长、第一和第二叶间距略长、穗颈和穗部较长的类型可能对提高产量有利。  相似文献   

"双链型"草-鹅-稻生态农业模式高产高效配套技术   总被引：11，自引：0，他引：11  

张家宏  王守红  白和盛  毕建花  张永新  唐宝国  孔佩  黄彩斌 《草业科学》2003,20(6):27-29

提出了“双链型”单-鹅-稻生态农业模式，总结了稻田套种牧草的方法和田间管理技术，探讨了种草与养鹅在时间上的相互衔接，以提高牧草的产量和利用率，进行了稻田套种牧草养鹅与种植小麦的比较效益分析。该配套技术的实施，为当前开展农业结构调整开辟了一条新路。  相似文献   

The effect of repeated administration of carbetocin (Depotocin, Spofa) in the first days postpartum on levels of thyroxine, triiodothyronine, 17 beta-estradiol, progesterone and on fertilization]     

E Bekeová  M Krajnicáková  V Hendrichovsky  I Maracek 《Veterinární medicína》1992,37(7):393-404

Application of new procedures in the sphere of the control of sexual functions requires an extension of present knowledge of postparturient endocrinium or endogenic factors comprised in postparturient physiology of sexual activity. According to recent data, oxytocin, besides its uterotonic and luteolytic activity, acts as an ovarian factor in the local intrafollicular regulation of stereidogenesis and as a modulator of uterine secretion of prostaglandines. Based on present knowledge of oxytocin effects, this study was aimed at investigation of the influence of repeated carbetocin (Depotocin inj. Spofa) administration on the dynamics of changes in thyroxine (T4), triiodothyronine (T3), 17 beta-estradiol (E2), progesterone (P4) concentrations and their mutual correlations from the 36th hour till the 51st day after parturition. Simultaneous study of a possible delayed influence of applied carbetocin on conception of ewes after oestrus evocation on day 51 after lambing was carried out. Nineteen ewes of the Slovak Merino breed, lambed in the first decade of February, were assigned to the experimental (n = 9) and to the control group (n = 10). Experimental ewes were subjected to repeated postparturient carbetocin treatment at the dose 0.07 mg per animal. The first dose was applied i. m. in 24 hours, and the second in 72 hours after parturition. On day 51 oestrus was induced in nine ewes of each group by combined treatment with chlorsuperlutin (Agelin, vaginal pessaries, Spofa) and PMSG (500 I.U./animal). On the day of PMSG application ewes were housed together with rams for the period of the next six days. Samples of blood were taken 24 hours before parturition (-1st day), up to 36 h after parturition and on days 4, 7, 14, 17, 21, 25, 34, 42 and 51 after parturition. Concentrations of T4, T3, E2 and P4 were determined by commercial kits RIA-test-T4; RIA-test-T3; RIA-test-ESTRA and RIA-test-PROG (URVJT Kosice). Animal of the control group showed variations of T4 concentrations (Tab. I, Fig. 1) at the level of original values (59.4 +/- 9.69 nmol.l-1) up to the 21st day with the exception of temporary drop on day 4 and rise on day 7, insignificant compared to the -1st day. T4 concentrations of the control group displayed an intermittent increasing trend with the statistically insignificant peak after 36 h and on day 17, compared to the -1st day. After the 21st day controls revealed a sustained moderate increase while the experimental ewes displayed a decline of its concentrations until the 51st day.(ABSTRACT TRUNCATED AT 400 WORDS)  相似文献   

Determination of sulfamethazine and trimethoprim in liquid feed premixes by HPLC and diode array detection, with an analysis of the uncertainty of the analytical results.     

B Cancho Grande  M S García Falcón  M Rodríguez Comesa?a  J Simal Gándara 《Journal of agricultural and food chemistry》2001,49(7):3145-3150

Sulfamethazine (SMZ) and trimethoprim (TMP) are antibacterials used in veterinary practice. This paper describes a method for their determination in veterinary liquid feed premixes that is based on liquid chromatography with diode array detection. Gradient elution with methanol and ammonium acetate achieved excellent separation of the two analytes within 15 min without any interference from the matrix. Absorbance of the column effluent was monitored at 264 nm for SMZ and at 230 nm for TMP. Detailed analyses of the uncertainties of determinations afford estimated expanded uncertainties of, respectively, 0.2 and 0.1 w/v % for typical SMZ and TMP concentrations of 10.7 and 2.1 w/v %, respectively. At the lower end of the calibrated range of the method, the dominant source of uncertainty is the preparation of standards and the construction of the calibration line.  相似文献   

沙棘叶中4，4′-二羟基-2′-甲氧基查耳酮的分离与鉴定     

阮栋梁  陶海荣李和 《沙棘》2004,17(1):24-27

利用硅胶柱和葡聚糖凝胶柱层析，从沙棘叶子中分离并鉴定出黄酮类成分，进一步分离得到4，4′-二羟基-2′-甲氧基查耳酮，并对其结构进行了结构鉴定。  相似文献   

Estradiol-17beta in the blood of boars with disorders of sexual potency     

S Navrátil  P Forejtek 《Veterinární medicína》1986,31(11):681-686

The levels of oestradiol-17 beta (E2) in the blood plasma were evaluated in 15 boars exhibiting signs of sexual potency disorders and in 20 boars with no such disorders before and after i. v. administration of chorion gonadotropin (HCG). No significant difference (P greater than 0.05) was found in the E2 levels in the blood plasma of the boars of the two groups before HCG administration. Two hours after the i. v. injection of 500 I. U. of HCG, an insignificant increase in the basal levels of E2 was recorded, reaching on the average 28.9% in the boars with potency disorders and 38.8% in those with no potency changes. Neither were there any significant differences in the E2 levels determined after HCG treatment between the boars with and without sexual potency disorders. It is inferred from the results that deviations in E2 concentration in the blood obviously do not contribute significantly to disorders in the sexual potency of boars.  相似文献   

江西板栗疫病发生规律的研究   总被引：5，自引：1，他引：4  

游春平  唐祥宁 《江西农业大学学报》1995,17(1):13-16

  相似文献   

应用生物素标记的NDV－cDNA探针检测感染尿囊液中NDV－RNA的初步研究     

陈书琨  何云生  李观娣  钟安清 《畜牧兽医学报》1995,(4)

本文应用聚合酶链反应（ＰＣＲ）技术从构建的新城疫病毒（ＮＤＶ）ｃＤＮＡ文库中扩增含编码Ｆ糖蛋白前体──Ｆｏ酶切位点序列的３５９ｂｐ的Ｆ蛋白基因ｃＤＮＡ片段。将此３５９ｂｐｃＤＮＡ片段经光敏生物素标记后,即成ＮＤＶ－ｃＤＮＡ探针。该探针能特异性地从感染的尿囊液中检测出ＮＤＶ强毒株和疫苗毒株的基因组ＲＮＡ,而不与ＩＢＤｖ－ｄｓＲＮＡ、ＡＩＢｖ－ｓｓＲＮＡ、ＥＤＳ７６－ｄｓＤＮＡ、ＭＤＶ－ｄｓＤＮＡ,ＦＰＶ－ｄｓＲＮＡ及ＡＩＬＶ－ｄｓＤＮＡ发生交叉杂交反应。试验结果表明：尽管该探钎含有编码Ｆｏ蛋白酶切位点序列的碱基顺序,但它还是不能把ＮＤＶ的强、弱毒株区分开。这说明ＮＤＶ强、弱毒株比区域内的碱基存在着相当大的同源性。不过,此探针对ＮＤＶ来说具有特异性,这就为ＮＤＶ的诊断技术开创了基因水平检测的新途径。  相似文献   

Dynamics of chlorinated pesticide residues in long-term storage of canned food     

J Garcár  J Hrusovsky  M Smirják 《Veterinární medicína》1987,32(9):547-555

Meat tins denoted as pork in natural juice, beef with bacon, luncheon meat and liver paté, all produced in a food-processing plant, were stored under definite conditions (temperature 21 degrees C, relative humidity 73%) for three years. Before the long-term storage and then in half-year intervals, the contents of the residues of chlorinated pesticides, including HCB (hexachlorobenzene), gamma-HCH (gamma isomer of hexachlorocyclohexane) and pp'DDE (1,1-dichloro-2,2-bis/p-chlorofenyl/-ethylene), were determined in the above-mentioned final products by the method of gas chromatography. As suggested by the records of the changes in the pesticide residue contents in the meat tins during their long-term storage, the average contents of all chlorinated carbohydrates gradually decrease with the length of storage and the "reduction" of residues varies with the kind of final product and with the pesticides. In HCB the reduction ranged from 18.4 to 34.4%, in gamma-HCH from 36.2 to 80.4% and in pp'DDE from 23.9 to 48.9%.  相似文献